Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021136
(805 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NA... 117 8e-27
At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NA... 116 1e-26
At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NA... 113 2e-25
At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NA... 109 3e-24
At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NA... 107 1e-23
At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NA... 103 1e-22
At1g32480.1 68414.m04008 isocitrate/isopropylmalate dehydrogenas... 50 2e-06
At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase, chloro... 34 0.13
At1g80560.1 68414.m09445 3-isopropylmalate dehydrogenase, chloro... 33 0.17
At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase, chloro... 32 0.39
At1g14820.3 68414.m01773 SEC14 cytosolic factor family protein /... 30 1.6
At1g14820.2 68414.m01772 SEC14 cytosolic factor family protein /... 30 1.6
At1g14820.1 68414.m01771 SEC14 cytosolic factor family protein /... 30 1.6
At4g23740.1 68417.m03415 leucine-rich repeat transmembrane prote... 30 2.1
At3g01030.1 68416.m00004 zinc finger (C2H2 type) family protein ... 30 2.1
At5g16280.1 68418.m01901 expressed protein 29 4.8
At4g14940.1 68417.m02294 copper amine oxidase, putative highly s... 28 8.3
>At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 368
Score = 117 bits (282), Expect = 8e-27
Identities = 77/185 (41%), Positives = 105/185 (56%), Gaps = 7/185 (3%)
Frame = +2
Query: 269 TLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIK 448
TLIPGDG+GP + AV++V +A PV FE + EV + E+V+ S+ NK+C+K
Sbjct: 42 TLIPGDGIGPLVTGAVEQVMEAMHAPVHFERY---EVLGNMRKVPEEVIESVKRNKVCLK 98
Query: 449 GILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHHH-------QR 607
G LATP G + +LNM+ L ++ ++ + PG H +
Sbjct: 99 GGLATPV---GGGVSSLNMQLRKE----LDIFASLV--NCINVPGLVTRHENVDIVVIRE 149
Query: 608 TDRREYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIMKLG 787
EYS LEHE VPGVVE LK+IT SERIA++AF+YA + ++ KANIMKL
Sbjct: 150 NTEGEYSGLEHEVVPGVVESLKVITKFCSERIARYAFEYAYLNNRKKVTAVHKANIMKLA 209
Query: 788 DGLFL 802
DGLFL
Sbjct: 210 DGLFL 214
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +3
Query: 552 SLPNVKCRHQDVDCIIIREQTE 617
++P + RH++VD ++IRE TE
Sbjct: 131 NVPGLVTRHENVDIVVIRENTE 152
>At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 367
Score = 116 bits (280), Expect = 1e-26
Identities = 76/180 (42%), Positives = 102/180 (56%), Gaps = 2/180 (1%)
Frame = +2
Query: 269 TLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIK 448
TLIPGDGVGP + AVQ+V +A PV FE F EV+ + + E ++ SI NK+C+K
Sbjct: 41 TLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPF---EVHGDMKSLPEGLLESIKKNKVCLK 97
Query: 449 GILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEV--IAQREVQAPGRGLHHHQRTDRRE 622
G L TP L +N++ + +L + +A R + + E
Sbjct: 98 GGLKTPVGGGVSSLN-VNLRKELDLFASLVNCFNLPGLASRHENVD---IVVIRENTEGE 153
Query: 623 YSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIMKLGDGLFL 802
Y+ LEHE VPGVVE LK+IT SERIAK+AF+YA + ++ KANIMKL DGLFL
Sbjct: 154 YAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFL 213
Score = 30.7 bits (66), Expect = 1.2
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +3
Query: 552 SLPNVKCRHQDVDCIIIREQTE 617
+LP + RH++VD ++IRE TE
Sbjct: 130 NLPGLASRHENVDIVVIRENTE 151
>At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NAD+
isocitrate dehydrogenase subunit 1 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 367
Score = 113 bits (271), Expect = 2e-25
Identities = 69/178 (38%), Positives = 99/178 (55%)
Frame = +2
Query: 269 TLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIK 448
TLIPGDG+GP + AV++V +A P+ FE + +V+ +S +V+ SI NK+C+K
Sbjct: 41 TLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKY---DVHGEMSRVPPEVMESIRKNKVCLK 97
Query: 449 GILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHHHQRTDRREYS 628
G L TP L K + + + ++ + + + + EY+
Sbjct: 98 GGLKTPVGGGVSSLNVQLRKELDLFASLVNCFN--LPGLPTRHENVDIVVIRENTEGEYA 155
Query: 629 ALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIMKLGDGLFL 802
LEHE VPGVVE LK+IT SERIAK+AF+YA + ++ KANIMKL DGLFL
Sbjct: 156 GLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFL 213
Score = 30.7 bits (66), Expect = 1.2
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +3
Query: 552 SLPNVKCRHQDVDCIIIREQTE 617
+LP + RH++VD ++IRE TE
Sbjct: 130 NLPGLPTRHENVDIVVIRENTE 151
>At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) GB:CAA65502 GI:3021506
[Nicotiana tabacum]
Length = 374
Score = 109 bits (261), Expect = 3e-24
Identities = 71/184 (38%), Positives = 102/184 (55%), Gaps = 2/184 (1%)
Frame = +2
Query: 260 IKCTLIPGDGVGPELVYAVQEVFKAASIPVDF-ESFFFSEVNPTLSAPLE-DVVNSIAVN 433
IK TL PGDG+GPE+ +V++VF AA + +D+ E F +EV+P ++ L D + S+ N
Sbjct: 44 IKATLFPGDGIGPEIAESVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKN 103
Query: 434 KICIKGILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHHHQRTD 613
K+ +KG +ATP L K + + YS + + + L +
Sbjct: 104 KVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYS--LPGYKTRYDDVDLITIRENT 161
Query: 614 RREYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIMKLGDG 793
EYS LEH+ V GVVE LKIIT S R+A++AF YA G ++ KANIM+ DG
Sbjct: 162 EGEYSGLEHQVVKGVVESLKIITRKASMRVAEYAFLYAKTHGRKKVSAIHKANIMQKTDG 221
Query: 794 LFLR 805
LFL+
Sbjct: 222 LFLQ 225
>At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 363
Score = 107 bits (256), Expect = 1e-23
Identities = 74/180 (41%), Positives = 99/180 (55%), Gaps = 2/180 (1%)
Frame = +2
Query: 269 TLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIK 448
TLIPGDGVGP + AVQ+V +A PV FE F EV+ + + E ++ SI NK+C+K
Sbjct: 41 TLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPF---EVHGDMKSLPEGLLESIKKNKVCLK 97
Query: 449 GILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEV--IAQREVQAPGRGLHHHQRTDRRE 622
G L TP L +N++ + +L + +A R + + E
Sbjct: 98 GGLKTPVGGGVSSLN-VNLRKELDLFASLVNCFNLPGLASRHENVD---IVVIRENTEGE 153
Query: 623 YSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIMKLGDGLFL 802
Y+ LEHE VPGVVE LK SERIAK+AF+YA + ++ KANIMKL DGLFL
Sbjct: 154 YAGLEHEVVPGVVESLKFC----SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFL 209
Score = 30.7 bits (66), Expect = 1.2
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +3
Query: 552 SLPNVKCRHQDVDCIIIREQTE 617
+LP + RH++VD ++IRE TE
Sbjct: 130 NLPGLASRHENVDIVVIRENTE 151
>At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
GI:3021506
Length = 374
Score = 103 bits (247), Expect = 1e-22
Identities = 66/184 (35%), Positives = 99/184 (53%), Gaps = 2/184 (1%)
Frame = +2
Query: 260 IKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFF-SEVNPTLSAPLE-DVVNSIAVN 433
I TL PGDG+GPE+ +V++VF A +P+++E + +E++P + L + + S+ N
Sbjct: 44 ITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRN 103
Query: 434 KICIKGILATPDFSHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHHHQRTD 613
K+ +KG +ATP L K + + YS + + + L +
Sbjct: 104 KVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYS--LPGYKTRYDDVDLITIRENT 161
Query: 614 RREYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIMKLGDG 793
EYS LEH+ V GVVE LKIIT S R+A++AF YA G + KANIM+ DG
Sbjct: 162 EGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDG 221
Query: 794 LFLR 805
LFL+
Sbjct: 222 LFLK 225
>At1g32480.1 68414.m04008 isocitrate/isopropylmalate dehydrogenase
family protein similar to NAD+ dependent isocitrate
dehydrogenase subunit 2 [Arabidopsis thaliana]
GI:1766048; contains Pfam profile PF00180 dehydrogenase,
isocitrate/isopropylmalate family
Length = 294
Score = 50.0 bits (114), Expect = 2e-06
Identities = 41/140 (29%), Positives = 67/140 (47%), Gaps = 1/140 (0%)
Frame = +2
Query: 311 AVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGEL 490
AV +V A PV FE++ N ++ +VV+SI NK+C+ G + G
Sbjct: 15 AVHQVMDAMQAPVYFETYIIKGKN--MNHLTWEVVDSIRKNKVCLNGRVNNSLCG--GAR 70
Query: 491 QTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHHHQRTDRREYSALEHESVPGVVECL 670
+ L++ + + F L ++ + EY+ EHE VPGV+E
Sbjct: 71 KELDLFASLVDCFNLNGQPSRHENVDIVVI-------RENTEGEYAGREHEVVPGVIESF 123
Query: 671 KI-ITAAKSERIAKFAFDYA 727
++ +T S+RIAK+AF+YA
Sbjct: 124 QVTMTKFWSDRIAKYAFEYA 143
>At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}; EST gb|F14478 comes from this gene
Length = 404
Score = 33.9 bits (74), Expect = 0.13
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +2
Query: 257 RIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV 379
R TL+PGDG+GPE++ + V + A E F F E+
Sbjct: 43 RYNITLLPGDGIGPEVISVAKNVLQKAGFLQGLE-FDFQEM 82
>At1g80560.1 68414.m09445 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to
3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR GB:P29102
SP|P29102 from [Brassica napus]
Length = 405
Score = 33.5 bits (73), Expect = 0.17
Identities = 18/41 (43%), Positives = 24/41 (58%)
Frame = +2
Query: 215 CTIWSPATEGNKGSRIKCTLIPGDGVGPELVYAVQEVFKAA 337
C + SP G K R TL+PGDG+GPE+V + V + A
Sbjct: 33 CAVASP---GKK--RYTITLLPGDGIGPEVVSIAKNVLQQA 68
>At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}
Length = 409
Score = 32.3 bits (70), Expect = 0.39
Identities = 21/58 (36%), Positives = 30/58 (51%)
Frame = +2
Query: 233 ATEGNKGSRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLE 406
A+ G K R L+PGDG+GPE++ + V + A E F F E+ P A L+
Sbjct: 40 ASPGKK--RYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLE-FDFKEM-PVGGAALD 93
>At1g14820.3 68414.m01773 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 252
Score = 30.3 bits (65), Expect = 1.6
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +2
Query: 302 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 451
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 35 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 84
>At1g14820.2 68414.m01772 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 239
Score = 30.3 bits (65), Expect = 1.6
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +2
Query: 302 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 451
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 22 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 71
>At1g14820.1 68414.m01771 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 239
Score = 30.3 bits (65), Expect = 1.6
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +2
Query: 302 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 451
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 22 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 71
>At4g23740.1 68417.m03415 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase -
Arabidopsis thaliana RKL1, PID:g4008006
Length = 638
Score = 29.9 bits (64), Expect = 2.1
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +2
Query: 191 NNREKCMLCTIWSPATEGNKGSRIKCTLIPGDGVGPEL 304
N E +C IW+ T GSRI +PG G+ ++
Sbjct: 47 NWNETSQVCNIWTGVTCNQDGSRIIAVRLPGVGLNGQI 84
>At3g01030.1 68416.m00004 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 353
Score = 29.9 bits (64), Expect = 2.1
Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
Frame = +1
Query: 394 CTTRRRRK--LYRC*QDLYQGYLSHS*LLSHWRATDPKHEVRNALDLYANVVQ 546
CT RRR K L RC ++ +G+L L +H + T + R +L ++VQ
Sbjct: 67 CTRRRRNKKILIRC-KECGKGFLYEKCLFNHLQVTHSEESTRRSLFCRFSIVQ 118
>At5g16280.1 68418.m01901 expressed protein
Length = 1265
Score = 28.7 bits (61), Expect = 4.8
Identities = 24/103 (23%), Positives = 45/103 (43%)
Frame = +2
Query: 473 SHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQAPGRGLHHHQRTDRREYSALEHESVP 652
+ GE ++KS P I +++++ E + ++ + R S +HE+
Sbjct: 97 TQAGEKDFQDLKSDPPQI------TDILSNPESEIAPSWFRYYNKELIRTLSFSDHEAFD 150
Query: 653 GVVECLKIITAAKSERIAKFAFDYAVKDGPQEGDRRPKANIMK 781
V CL ++++ E I KF + P +G P NI+K
Sbjct: 151 HPVACLLVVSSKDEEPIKKFVDLFNSNRLPTDGTMDP--NILK 191
>At4g14940.1 68417.m02294 copper amine oxidase, putative highly
similar to copper amine oxidase [Arabidopsis thaliana]
gi|2654118|gb|AAB87690
Length = 650
Score = 27.9 bits (59), Expect = 8.3
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 455 LATPDFSHTGELQTLNMKSVMPWIFTLTWYSEVIAQREVQA 577
L P F + ++LN+ V FT+ WY E +RE++A
Sbjct: 134 LTYPPFKKSILDRSLNISEVSCIPFTVGWYGETTTRRELKA 174
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,001,804
Number of Sequences: 28952
Number of extensions: 396186
Number of successful extensions: 1011
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 999
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1824072800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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