BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021129 (714 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 127 1e-30 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 125 7e-30 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 110 2e-25 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 108 9e-25 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 86 4e-18 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 51 1e-07 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 1e-04 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.29 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.87 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 28 1.5 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 28 1.5 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 28 1.5 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 6.1 SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|c... 25 8.1 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 127 bits (307), Expect = 1e-30 Identities = 71/139 (51%), Positives = 81/139 (58%) Frame = +3 Query: 261 IXDAKRLIGRKFEDATVQAVMKHWPFDXCQ*WRQT*VQGSI*G*RQNLFPRGSQFHGAYE 440 I DAKRLIGRKF+D VQ+ MKHWPF + +Q G + P + Sbjct: 65 IFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMK 124 Query: 441 NEGNCRSLSRQNCAECSYHGSRVFNDSHRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 + + FNDS RQATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 125 MRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLD 184 Query: 621 KKGTGERNVLIFDLGGGTF 677 + GE NVLIFDLGGGTF Sbjct: 185 RSNQGESNVLIFDLGGGTF 203 Score = 112 bits (269), Expect = 5e-26 Identities = 51/58 (87%), Positives = 55/58 (94%) Frame = +2 Query: 80 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 Score = 76.6 bits (180), Expect = 3e-15 Identities = 50/123 (40%), Positives = 59/123 (47%) Frame = +1 Query: 346 VSDGGKPKFKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVLASSMTLI 525 +S GKP +V YKGE KTF PEE+SSMVL KM+ETAEAYLG V +AV+TV A Sbjct: 93 ISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQ 152 Query: 526 DKPQKMQVPSQA*TFSESSMNPXXXXXXXXXXXXXXENEMYLSLTSAAVPFDVSILTIED 705 + K P L FDVS+LTIE+ Sbjct: 153 RQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSLLTIEE 212 Query: 706 GIF 714 GIF Sbjct: 213 GIF 215 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 125 bits (301), Expect = 7e-30 Identities = 69/139 (49%), Positives = 81/139 (58%) Frame = +3 Query: 261 IXDAKRLIGRKFEDATVQAVMKHWPFDXCQ*WRQT*VQGSI*G*RQNLFPRGSQFHGAYE 440 I DAKRLIGR+F D VQ+ MKHWPF + + +Q G + P + Sbjct: 65 IFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLK 124 Query: 441 NEGNCRSLSRQNCAECSYHGSRVFNDSHRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 + + + FNDS RQATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 125 MRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLD 184 Query: 621 KKGTGERNVLIFDLGGGTF 677 + E NVLIFDLGGGTF Sbjct: 185 RSNQHETNVLIFDLGGGTF 203 Score = 112 bits (269), Expect = 5e-26 Identities = 51/58 (87%), Positives = 55/58 (94%) Frame = +2 Query: 80 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 Score = 70.5 bits (165), Expect = 2e-13 Identities = 46/123 (37%), Positives = 58/123 (47%) Frame = +1 Query: 346 VSDGGKPKFKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVLASSMTLI 525 + GKP +V +KGE KTF PEE+SSMVL KM+E+AEA+LG V +AV+TV A Sbjct: 93 IEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQ 152 Query: 526 DKPQKMQVPSQA*TFSESSMNPXXXXXXXXXXXXXXENEMYLSLTSAAVPFDVSILTIED 705 + K P L FDVS+LTIE+ Sbjct: 153 RQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSLLTIEE 212 Query: 706 GIF 714 GIF Sbjct: 213 GIF 215 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 110 bits (264), Expect = 2e-25 Identities = 62/139 (44%), Positives = 80/139 (57%) Frame = +3 Query: 261 IXDAKRLIGRKFEDATVQAVMKHWPFDXCQ*WRQT*VQGSI*G*RQNLFPRGSQFHGAYE 440 + DAKRLIGR++ED Q +KHWPF ++ + G ++ + + Sbjct: 69 VFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTK 128 Query: 441 NEGNCRSLSRQNCAECSYHGSRVFNDSHRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 + + + + F+DS R ATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 129 MKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLD 188 Query: 621 KKGTGERNVLIFDLGGGTF 677 K +NVLIFDLGGGTF Sbjct: 189 AKSDKPKNVLIFDLGGGTF 207 Score = 100 bits (239), Expect = 2e-22 Identities = 45/58 (77%), Positives = 51/58 (87%) Frame = +2 Query: 80 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65 Score = 58.0 bits (134), Expect = 1e-09 Identities = 29/54 (53%), Positives = 37/54 (68%) Frame = +1 Query: 346 VSDGGKPKFKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVLA 507 + + G P +V Y GE K F +E+S+MVLTKMKE +EA L K V+ AVITV A Sbjct: 97 IDNNGIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVPA 150 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 108 bits (259), Expect = 9e-25 Identities = 59/139 (42%), Positives = 84/139 (60%) Frame = +3 Query: 261 IXDAKRLIGRKFEDATVQAVMKHWPFDXCQ*WRQT*VQGSI*G*RQNLFPRGSQFHGAYE 440 I D KRLIGRKF++ T+ +K +PF + V+ ++ G ++ P + Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSK 157 Query: 441 NEGNCRSLSRQNCAECSYHGSRVFNDSHRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 + + + FND+ RQATKDAGTI+GLNV+RI+NEPTAAAIAYGLD Sbjct: 158 MKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLD 217 Query: 621 KKGTGERNVLIFDLGGGTF 677 K T E++++++DLGGGTF Sbjct: 218 KTDT-EKHIVVYDLGGGTF 235 Score = 98.3 bits (234), Expect = 1e-21 Identities = 43/57 (75%), Positives = 50/57 (87%) Frame = +2 Query: 83 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNP 94 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 86.2 bits (204), Expect = 4e-18 Identities = 41/56 (73%), Positives = 47/56 (83%) Frame = +3 Query: 510 FNDSHRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTF 677 FNDS RQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGTF Sbjct: 194 FNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGTF 247 Score = 72.9 bits (171), Expect = 4e-14 Identities = 34/62 (54%), Positives = 44/62 (70%), Gaps = 1/62 (1%) Frame = +2 Query: 71 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 247 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 248 NP 253 NP Sbjct: 109 NP 110 Score = 48.4 bits (110), Expect = 1e-06 Identities = 20/37 (54%), Positives = 28/37 (75%) Frame = +1 Query: 397 KTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVLA 507 KT+ P ++ +L+KM+ETA YLGK V+NAV+TV A Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVPA 192 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +3 Query: 273 KRLIGRKFEDATVQAVMKHWPF 338 KRLIGR+F++ VQ +K P+ Sbjct: 118 KRLIGRRFKEPEVQRDIKEVPY 139 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 51.2 bits (117), Expect = 1e-07 Identities = 21/61 (34%), Positives = 39/61 (63%) Frame = +2 Query: 68 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 247 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 248 N 250 N Sbjct: 63 N 63 Score = 44.4 bits (100), Expect = 2e-05 Identities = 24/60 (40%), Positives = 33/60 (55%), Gaps = 4/60 (6%) Frame = +3 Query: 510 FNDSHRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGGTF 677 F D R+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G + Sbjct: 152 FTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNY 211 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/56 (30%), Positives = 31/56 (55%) Frame = +2 Query: 83 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 250 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRN 82 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 30.3 bits (65), Expect = 0.29 Identities = 19/63 (30%), Positives = 27/63 (42%), Gaps = 1/63 (1%) Frame = +2 Query: 83 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTT 259 + ID GT ++ + + G +EI+ R S VAF ER+ G A N P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPAH 84 Query: 260 DXR 268 R Sbjct: 85 SIR 87 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.87 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 75 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 170 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 27.9 bits (59), Expect = 1.5 Identities = 21/80 (26%), Positives = 35/80 (43%) Frame = +1 Query: 61 QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 240 +NGK T +R +S + W P++E R G D + V R + + +EP Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531 Query: 241 GDEPHNRXSMPNGSSDVSSK 300 ++P S+P S+ K Sbjct: 532 -EKPEEIPSVPPLPSNFKDK 550 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -2 Query: 551 GTCIFCGLSMRVIEDARTVITAFCTVLPR*ASAVSFIF 438 G C+FCGL+ D+ T+ C R A+ +S ++ Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -2 Query: 551 GTCIFCGLSMRVIEDARTVITAFCTVLPR*ASAVSFIF 438 G C+FCGL+ D+ T+ C R A+ +S ++ Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.8 bits (54), Expect = 6.1 Identities = 16/53 (30%), Positives = 26/53 (49%) Frame = -2 Query: 161 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 3 G W SP S + H ++++ +F +RVL C+ L K +K+A L Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323 >SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|chr 3|||Manual Length = 479 Score = 25.4 bits (53), Expect = 8.1 Identities = 15/61 (24%), Positives = 27/61 (44%), Gaps = 1/61 (1%) Frame = -1 Query: 552 WYLHLLWLVYESH*RRENRDNCILHSFAEISFGSFLHFRKHHGTDFLGEKGFVF-TLICY 376 WY H+ W++ + RR R + ++++ F+ F H F+F +L C Sbjct: 168 WYAHVGWMIILQNPRRIGRSD-----VSDLNSDPFVMFNHRHFLPIASFMAFIFPSLFCG 222 Query: 375 L 373 L Sbjct: 223 L 223 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,119,022 Number of Sequences: 5004 Number of extensions: 66605 Number of successful extensions: 232 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 202 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 225 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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