Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021127
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 56 2e-08
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 56 2e-08
At3g04940.1 68416.m00536 cysteine synthase, putative / O-acetyls... 28 5.0
At5g14080.1 68418.m01647 pentatricopeptide (PPR) repeat-containi... 28 6.6
At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S), ... 27 8.8
At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative / arg... 27 8.8
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 27 8.8
At4g20730.1 68417.m03013 filament protein-related similar to Cyt... 27 8.8
At1g15530.1 68414.m01868 receptor lectin kinase, putative simila... 27 8.8
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 56.4 bits (130), Expect = 2e-08
Identities = 32/71 (45%), Positives = 42/71 (59%)
Frame = +3
Query: 42 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 221
MS K EKP L G RIKTRKR+ DP F DA+VQ GDL+ K ++S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57
Query: 222 DYRCYGEVIFD 254
++ YG++ F+
Sbjct: 58 NFTRYGDIFFE 68
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +2
Query: 389 KLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTA 508
K++RR +L K E ++ L L+ F+ +R KLA TA
Sbjct: 112 KILRRKPFLIKNLENVTRRFLQSLELFEENERKKLAIFTA 151
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 56.0 bits (129), Expect = 2e-08
Identities = 33/71 (46%), Positives = 42/71 (59%)
Frame = +3
Query: 42 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 221
MS K EKP L G RIKTRKR+ DP F DALVQ GDL+ + L+S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57
Query: 222 DYRCYGEVIFD 254
++ YG++ F+
Sbjct: 58 NFSRYGDIFFE 68
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +2
Query: 389 KLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTA 508
K++RR +L K E ++ L L+ F+ +R KLA TA
Sbjct: 112 KILRRKAFLIKNLENVTRRFLQSLELFEENERKKLAIFTA 151
>At3g04940.1 68416.m00536 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 324
Score = 28.3 bits (60), Expect = 5.0
Identities = 26/102 (25%), Positives = 46/102 (45%), Gaps = 2/102 (1%)
Frame = +1
Query: 382 FRETDASVQVLGKNVRRGNE-KGSGLLERLRSRTAHQAGAHDC-MWIGNGCVPTSVLLVL 555
+R++ V +L V G GSG + +++ G + +G P + L+
Sbjct: 169 WRDSAGEVDILVAGVGTGGTLSGSGRFLKEKNKDFKVYGVEPTESAVISGGKPGTHLIQG 228
Query: 556 VNEHLLKDNLALDFVLEVFAIIKHERGVTSLVAALKKGQLEG 681
+ L+ DNL + + EV + E T+ + ALK+G L G
Sbjct: 229 IGAGLIPDNLDFNVLDEVIQVTSVEAIETAKLLALKEGLLVG 270
>At5g14080.1 68418.m01647 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 634
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = -1
Query: 684 SAFKLSLLERRDERGDAALMLDDREHFQHEVQRQVVLQQMFVHQDQQHGCRH 529
S F L+L G+A+ +L +REH +H V+L+ + ++ + G RH
Sbjct: 542 SEFVLNLCSN-GHSGEASQLLREREHLEHTGAHVVLLKCVADAKEVEIGIRH 592
>At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S),
Arabidopsis thaliana, PIR:B71412
Length = 303
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/42 (40%), Positives = 26/42 (61%)
Frame = -3
Query: 616 SRTLPARSPAPGCPSADVRSPGPAARM*ARIRYRSTCSHARQ 491
SR+L + SP+PG S+ +R PAA+ R ++ S S+ RQ
Sbjct: 18 SRSLASSSPSPGSFSSSMRLWRPAAQRNLRNQW-SNLSNCRQ 58
>At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative /
arginine--tRNA ligase, putative similar to SP|P37880
Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine--tRNA
ligase) (ArgRS) {Cricetulus longicaudatus}; contains
Pfam profiles PF00750: arginyl-tRNA synthetase, PF03485:
arginyl-tRNA synthetase N-terminal domain
Length = 642
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +2
Query: 377 QVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARM 502
QVF K + L++ F+E+ ++ +V L+G DP R A++
Sbjct: 260 QVFYKASKHKFDLDEAFKEKAQQAVVRLQGGDPVYRKAWAKI 301
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +3
Query: 102 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 197
D K YD N R L Q +E AGG + A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269
>At4g20730.1 68417.m03013 filament protein-related similar to
Cytadherence high molecular weight protein 2 (SP:P47460)
[Mycoplasma genitalium]; similar to YEAST NUF1
protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; also
SP|Q9UKX2|MYH2_HUMAN Myosin heavy chain, skeletal
muscle, SP|P31732|OV71_ONCVO Muscle cell intermediate
filament protein SP|P12882|MYH1_HUMAN Myosin heavy
chain, skeletal muscle,. SP|Q17107|AV71_ACAVI Muscle
cell intermediate filament protein
Length = 800
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +1
Query: 388 ETDASVQVLGKNVRRGNEKGSGLLERLRSRTAHQAG 495
+ D +V LG + R EKG G+ + + R+A +AG
Sbjct: 455 DKDETVAELGISRRSREEKGKGVATQSKKRSASEAG 490
>At1g15530.1 68414.m01868 receptor lectin kinase, putative similar
to receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains pfam domains PF00139:
Legume lectins beta domain and PF00069: Protein kinase
domain
Length = 656
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/36 (30%), Positives = 22/36 (61%)
Frame = +1
Query: 532 PTSVLLVLVNEHLLKDNLALDFVLEVFAIIKHERGV 639
P+ +LL+L+ HL+ + A+DF+ F+ + + V
Sbjct: 11 PSPLLLILIVLHLVSSSSAIDFLYNSFSSVTNRTDV 46
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,737,070
Number of Sequences: 28952
Number of extensions: 323617
Number of successful extensions: 1137
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1071
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1137
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -