BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021120 (825 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AJ439060-7|CAD27758.1| 849|Anopheles gambiae putative V-ATPase ... 108 2e-25 AJ439060-8|CAD27759.1| 808|Anopheles gambiae putative V-ATPase ... 99 2e-22 >AJ439060-7|CAD27758.1| 849|Anopheles gambiae putative V-ATPase protein. Length = 849 Score = 108 bits (259), Expect = 2e-25 Identities = 48/76 (63%), Positives = 57/76 (75%) Frame = +3 Query: 255 GLMSNDYQGGIFLYVVFAGWAAISVSILVLMEGLSAFLHTLRLHWVEFQSKFYGGEGYLF 434 GL Y+G I LY VF W+ +++ILV+MEGLSAFLHTLRLHWVEF SKFY G GY F Sbjct: 774 GLKQTSYKGAIMLYFVFGAWSLFTLAILVMMEGLSAFLHTLRLHWVEFMSKFYEGLGYGF 833 Query: 435 QPFSFEIILDSAGQAE 482 QPFSF++I+DS E Sbjct: 834 QPFSFKLIIDSDDDLE 849 Score = 98.7 bits (235), Expect = 2e-22 Identities = 43/59 (72%), Positives = 50/59 (84%) Frame = +1 Query: 73 PVPASGHHDEEITEVFIHQAIHTIEFVLGSVSHTASYLRLWALSLAHAQLAEVAWNMLL 249 P P H DE + E+FIHQAIHTIE+VL +VSHTASYLRLWALSLAHA+L+EV WNM+L Sbjct: 713 PKPHDSHDDEPMAEIFIHQAIHTIEYVLSTVSHTASYLRLWALSLAHAELSEVLWNMVL 771 >AJ439060-8|CAD27759.1| 808|Anopheles gambiae putative V-ATPase protein. Length = 808 Score = 98.7 bits (235), Expect = 2e-22 Identities = 43/70 (61%), Positives = 55/70 (78%) Frame = +3 Query: 255 GLMSNDYQGGIFLYVVFAGWAAISVSILVLMEGLSAFLHTLRLHWVEFQSKFYGGEGYLF 434 GL ++ Y G I +++VF W+ +++ ILV MEGLSAFLHTLRLHWVEF SKFY G GY F Sbjct: 734 GLRNDSYVGAIMIWLVFWPWSVLTIGILVGMEGLSAFLHTLRLHWVEFMSKFYEGLGYAF 793 Query: 435 QPFSFEIILD 464 +PFSF+ IL+ Sbjct: 794 KPFSFKAILE 803 Score = 91.5 bits (217), Expect = 3e-20 Identities = 39/56 (69%), Positives = 52/56 (92%), Gaps = 1/56 (1%) Frame = +1 Query: 82 ASGHHDEE-ITEVFIHQAIHTIEFVLGSVSHTASYLRLWALSLAHAQLAEVAWNML 246 +S HHD+E ++E+FIHQAIHTIE++L ++SHTASYLRLWALSLAHA+L+EV +NM+ Sbjct: 675 SSNHHDDEPMSEIFIHQAIHTIEYILSTISHTASYLRLWALSLAHAELSEVLYNMV 730 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 679,970 Number of Sequences: 2352 Number of extensions: 12981 Number of successful extensions: 59 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 57 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 59 length of database: 563,979 effective HSP length: 63 effective length of database: 415,803 effective search space used: 87734433 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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