BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021118 (724 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 139 4e-34 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 91 1e-19 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 82 7e-17 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 66 4e-12 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 61 1e-10 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 49 8e-07 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 48 1e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 46 4e-06 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 27 2.7 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 4.7 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 26 6.3 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 139 bits (336), Expect = 4e-34 Identities = 63/72 (87%), Positives = 65/72 (90%) Frame = -1 Query: 724 KSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANT 545 KSYELPDGQVITIGNERFR PEALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N Sbjct: 238 KSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNV 297 Query: 544 VLSGGTTMYPGI 509 V+SGGTTMYPGI Sbjct: 298 VMSGGTTMYPGI 309 Score = 129 bits (312), Expect = 3e-31 Identities = 59/69 (85%), Positives = 63/69 (91%) Frame = -3 Query: 518 PWNPDRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSIXASLSTFQQMWISKQEYDESG 339 P DRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSI ASLSTFQQMWISKQEYDESG Sbjct: 307 PGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 366 Query: 338 PSIVHRKCF 312 P IV+RKCF Sbjct: 367 PGIVYRKCF 375 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 91.1 bits (216), Expect = 1e-19 Identities = 39/74 (52%), Positives = 54/74 (72%) Frame = -1 Query: 724 KSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANT 545 ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E +++I DVDIRK LY Sbjct: 238 RNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAI 297 Query: 544 VLSGGTTMYPGIPT 503 VLSGG++MY G+P+ Sbjct: 298 VLSGGSSMYAGLPS 311 Score = 39.9 bits (89), Expect = 4e-04 Identities = 17/42 (40%), Positives = 29/42 (69%), Gaps = 1/42 (2%) Frame = -3 Query: 461 KIKIIAPPERKYSVWIGGSIXAS-LSTFQQMWISKQEYDESG 339 K+KI P R+++V+IGG++ A ++ MW+SK E++E G Sbjct: 337 KVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 82.2 bits (194), Expect = 7e-17 Identities = 35/70 (50%), Positives = 47/70 (67%) Frame = -1 Query: 721 SYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTV 542 +Y+LPD Q IT+G E F PE LF P +G EA G+H + SI+ D+D+R LY+N V Sbjct: 244 TYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIV 303 Query: 541 LSGGTTMYPG 512 LSGG+T+ G Sbjct: 304 LSGGSTLLRG 313 Score = 56.8 bits (131), Expect = 3e-09 Identities = 25/65 (38%), Positives = 45/65 (69%) Frame = -3 Query: 506 DRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSIXASLSTFQQMWISKQEYDESGPSIV 327 +R E+ A++ ++KI A PER ++ W+GGSI ASLSTF+++ I+ +EY ++ +++ Sbjct: 316 ERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVI 374 Query: 326 HRKCF 312 R+ F Sbjct: 375 FRRRF 379 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 66.5 bits (155), Expect = 4e-12 Identities = 33/81 (40%), Positives = 44/81 (54%), Gaps = 9/81 (11%) Frame = -1 Query: 724 KSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC---------GIHETTYNSIMKCDVD 572 K +E PDG G ERFR E LF PSF + G+HE Y SI+ CD + Sbjct: 279 KPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSE 338 Query: 571 IRKDLYANTVLSGGTTMYPGI 509 +R L N V++GGT++ PG+ Sbjct: 339 LRSPLLNNIVVTGGTSLIPGL 359 Score = 52.8 bits (121), Expect = 5e-08 Identities = 27/63 (42%), Positives = 36/63 (57%), Gaps = 2/63 (3%) Frame = -3 Query: 521 VPWNPDRMQKEITALAP-STMKIKIIAPPER-KYSVWIGGSIXASLSTFQQMWISKQEYD 348 +P +R+Q E+ LA S + + +VW GGSI ASL FQ +W+SKQEYD Sbjct: 356 IPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEYD 415 Query: 347 ESG 339 E G Sbjct: 416 EVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 61.3 bits (142), Expect = 1e-10 Identities = 30/69 (43%), Positives = 47/69 (68%), Gaps = 6/69 (8%) Frame = -3 Query: 503 RMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSIXASLSTFQQMWISKQEYDESGP- 336 R+Q E++ L P + ++KI A ER Y+ W+GGSI +SL TF Q+WIS+QEY+E G Sbjct: 365 RLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSD 423 Query: 335 --SIVHRKC 315 +++ ++C Sbjct: 424 RLALIEKRC 432 Score = 37.1 bits (82), Expect(2) = 1e-06 Identities = 16/36 (44%), Positives = 22/36 (61%) Frame = -1 Query: 619 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPG 512 GI + N I +CDVDIR L N ++ GGT++ G Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQG 361 Score = 30.7 bits (66), Expect(2) = 1e-06 Identities = 12/25 (48%), Positives = 16/25 (64%) Frame = -1 Query: 718 YELPDGQVITIGNERFRCPEALFQP 644 +E PDG + G ER++ PE LF P Sbjct: 264 FEFPDGSRMMFGAERYQIPEHLFVP 288 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 48.8 bits (111), Expect = 8e-07 Identities = 24/63 (38%), Positives = 32/63 (50%) Frame = -1 Query: 700 QVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTM 521 QV+ + NERF PE LF PS + + GI E S+ +I+ L N V GG Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331 Query: 520 YPG 512 +PG Sbjct: 332 FPG 334 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 48.0 bits (109), Expect = 1e-06 Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 1/60 (1%) Frame = -1 Query: 694 ITIGNERFRCPEALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMY 518 I +G ERF PE F P + + E N + +D+RK LY N VLSGG+T++ Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLF 339 Score = 35.5 bits (78), Expect = 0.008 Identities = 15/46 (32%), Positives = 25/46 (54%) Frame = -3 Query: 458 IKIIAPPERKYSVWIGGSIXASLSTFQQMWISKQEYDESGPSIVHR 321 + +I+ ++ +VW GGS+ A F +K +Y+E G SI R Sbjct: 374 VNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 46.4 bits (105), Expect = 4e-06 Identities = 20/64 (31%), Positives = 38/64 (59%) Frame = -1 Query: 679 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIPTV 500 E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ T Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 499 CKRK 488 KR+ Sbjct: 654 IKRE 657 Score = 34.3 bits (75), Expect = 0.018 Identities = 15/65 (23%), Positives = 30/65 (46%) Frame = -3 Query: 521 VPWNPDRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSIXASLSTFQQMWISKQEYDES 342 +P R+++E+T++ P I + W G S + F+ ++++EY E Sbjct: 647 LPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEK 706 Query: 341 GPSIV 327 GP + Sbjct: 707 GPEYI 711 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.1 bits (57), Expect = 2.7 Identities = 12/37 (32%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = -3 Query: 419 WIGGSIXASLSTFQQM---WISKQEYDESGPSIVHRK 318 ++GGSI A S + + +++ +EY + GP+ +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -1 Query: 427 TPYGSVDRSXPPSLPSNRCGSRNRSTTSLAPP 332 TP + PPS+PS+R R S ++ APP Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.8 bits (54), Expect = 6.3 Identities = 12/64 (18%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Frame = -3 Query: 500 MQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSIXASLSTFQQMWISKQEYDESGPSI 330 +++ I + + I +I PP + ++ W G I + ++WI ++ G + Sbjct: 549 LEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIRIVSELWIKNSDWKMLGSRV 608 Query: 329 VHRK 318 + K Sbjct: 609 LQYK 612 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,761,038 Number of Sequences: 5004 Number of extensions: 56494 Number of successful extensions: 177 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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