Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021117
(817 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 103 3e-23
SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 53 6e-08
SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 39 7e-04
SPAC11E3.09 |pyp3||protein-tyrosine phosphatase Pyp3|Schizosacch... 28 1.8
SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 27 2.4
SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac... 27 2.4
SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe... 27 3.2
SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein... 27 3.2
SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomy... 26 5.6
SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|c... 26 7.4
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 25 9.7
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 25 9.7
>SPAC3F10.16c |||GTP binding protein,
HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
Length = 616
Score = 103 bits (247), Expect = 3e-23
Identities = 46/93 (49%), Positives = 65/93 (69%), Gaps = 2/93 (2%)
Frame = +2
Query: 512 VTPYERNLELWKQLWRTLEKSDIVLILLDARDPLLFRCVDLEKYAKE--QKCQSILLLNK 685
VTP+ERNLE+W+QLWR +E+SD+V+ ++DAR+PL FR LE+Y KE ++ LL+NK
Sbjct: 151 VTPFERNLEIWRQLWRVIERSDVVVQIVDARNPLFFRSAHLEQYVKEVGPSKKNFLLVNK 210
Query: 686 ADLTSEYERKCWAEYFNKGNIRISLFFGRQNYE 784
AD+ +E +R W+ YFN+ NI F R E
Sbjct: 211 ADMLTEEQRNYWSSYFNENNIPFLFFSARMAAE 243
Score = 62.9 bits (146), Expect = 5e-11
Identities = 35/88 (39%), Positives = 51/88 (57%), Gaps = 9/88 (10%)
Frame = +3
Query: 258 EFLSTAELAQREFTAEKLNLKYVKSVPSEVAIVTS---------QPDFDEPLTVPRRPQW 410
EFL+TAEL + EF AEK N+ +++ +++ Q + LT+PRRP W
Sbjct: 57 EFLNTAELGEVEFIAEKQNVTVIQNPEQNPFLLSKEEAARSKQKQEKNKDRLTIPRRPHW 116
Query: 411 NPGVTAEEQLNRERETFLDWRRHLNELQ 494
+ TA E ERE+FL+WRR+L +LQ
Sbjct: 117 DQTTTAVELDRMERESFLNWRRNLAQLQ 144
>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 537
Score = 52.8 bits (121), Expect = 6e-08
Identities = 23/69 (33%), Positives = 40/69 (57%), Gaps = 2/69 (2%)
Frame = +2
Query: 539 LWKQLWRTLEKSDIVLILLDARDPLLFRCVDLEKYAKEQKCQS--ILLLNKADLTSEYER 712
+W +L++ ++ SD+++ +LDARDP+ RC +E+Y + + IL+LNK DL
Sbjct: 206 IWNELYKVIDSSDVLIQVLDARDPVGTRCGTVERYLRNEASHKHMILVLNKVDLVPTSVA 265
Query: 713 KCWAEYFNK 739
W + K
Sbjct: 266 AAWVKILAK 274
>SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 470
Score = 39.1 bits (87), Expect = 7e-04
Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 3/65 (4%)
Frame = +2
Query: 545 KQLWRTLEKSDIVLILLDARDPLLFRCVDLEKY---AKEQKCQSILLLNKADLTSEYERK 715
K+ + +E SD++L +LDARDP R D+E+ + ++ + I ++NK DL
Sbjct: 154 KEFKKVVEASDVILYVLDARDPEGTRSKDVERQVLASSAEEKRLIFVINKIDLVPSEVLN 213
Query: 716 CWAEY 730
W Y
Sbjct: 214 KWVTY 218
>SPAC11E3.09 |pyp3||protein-tyrosine phosphatase
Pyp3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 303
Score = 27.9 bits (59), Expect = 1.8
Identities = 9/28 (32%), Positives = 18/28 (64%)
Frame = +2
Query: 542 WKQLWRTLEKSDIVLILLDARDPLLFRC 625
WK +W+++ KS I+++L R+ +C
Sbjct: 107 WKMVWQSVPKSGIIVMLTKLRERHRLKC 134
>SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 910
Score = 27.5 bits (58), Expect = 2.4
Identities = 19/70 (27%), Positives = 32/70 (45%)
Frame = -2
Query: 231 LEIQTSPIISIIHLCSVEHGIVFDMFFVAIFRESIFDEGSPKRLFILLAHYLNLHYKTGR 52
L T+P+ II S +G+++ V E + GSPK L +++ R
Sbjct: 176 LTFDTTPLFPIIKYDSENNGLMYKPMIVLHSNEFLLITGSPKDAIGL---FVDSQGNVTR 232
Query: 51 NTTSFNFSNK 22
+T +F+F K
Sbjct: 233 STLTFSFYPK 242
>SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor
CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 797
Score = 27.5 bits (58), Expect = 2.4
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -2
Query: 615 NKGSLASNNINTISDFSKVLH 553
N+ L NINTI+DFS++ H
Sbjct: 289 NENLLEFRNINTITDFSQISH 309
>SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 27.1 bits (57), Expect = 3.2
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 4/33 (12%)
Frame = -3
Query: 158 CFLLRFL----ENLSLMRALPRDSLFFLPIILT 72
CF LRF+ ++ SL R+LPR L L I+L+
Sbjct: 439 CFALRFILHNYKDFSLPRSLPRPFLLALSILLS 471
>SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 758
Score = 27.1 bits (57), Expect = 3.2
Identities = 12/49 (24%), Positives = 25/49 (51%)
Frame = +2
Query: 566 EKSDIVLILLDARDPLLFRCVDLEKYAKEQKCQSILLLNKADLTSEYER 712
E+ D+V+ +++A + D + A +K +++NK D + ER
Sbjct: 289 EEIDVVVFVVNAENHFTLSATDFLRNASTEKSHIFIIVNKFDNIRDKER 337
>SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 613
Score = 26.2 bits (55), Expect = 5.6
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -2
Query: 609 GSLASNNINTISDFSKV 559
G +A NN +TI+DFSK+
Sbjct: 167 GGIALNNFSTINDFSKL 183
>SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 479
Score = 25.8 bits (54), Expect = 7.4
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +3
Query: 387 TVPRRPQWNPGVTAEEQLNRERETFLDWRRHLNELQAKL 503
+VP R +W+P A + + + T +W RHLN L L
Sbjct: 34 SVPER-KWDP--KAPKHIQEQPWTMQNWWRHLNWLHCML 69
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 25.4 bits (53), Expect = 9.7
Identities = 12/63 (19%), Positives = 26/63 (41%)
Frame = +2
Query: 593 LDARDPLLFRCVDLEKYAKEQKCQSILLLNKADLTSEYERKCWAEYFNKGNIRISLFFGR 772
+D F C+ Y + I+ L+KA+ + + +Y++ I I + F +
Sbjct: 118 MDTFRTYFFNCLKESDYVRNGSSSGIIALSKAETDTYWNAILNHDYYDFRPIAIKILFSK 177
Query: 773 QNY 781
+
Sbjct: 178 SKF 180
>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 987
Score = 25.4 bits (53), Expect = 9.7
Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
Frame = -2
Query: 744 FPLLKYSAQHFLSYSLVRSALFSNNILW--HFCSLAYFSRSTHLNNKGSLASNNINTIS 574
FP L+ SA+ FL + L +NN L+ FC F +T G +N ++ +S
Sbjct: 308 FPRLQQSAEDFLYHLQFFFTLCANNSLYLSRFCYYPSFVPNTPFG--GKWTNNGLSAVS 364
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,372,742
Number of Sequences: 5004
Number of extensions: 71266
Number of successful extensions: 240
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 238
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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