BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021114 (695 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|S... 62 1e-10 SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 60 3e-10 SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 59 5e-10 SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharom... 51 2e-07 SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 46 4e-06 SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 46 5e-06 SPBC1604.20c |tea2|klp4|kinesin-like protein Tea2|Schizosaccharo... 40 3e-04 SPBC15D4.01c ||SPBC2D10.21c|kinesin-like protein|Schizosaccharom... 38 0.001 SPBC651.09c |||RNA polymerase II associated Paf1 complex |Schizo... 27 3.4 SPCPB16A4.03c |ade10||IMP cyclohydrolase|Schizosaccharomyces pom... 27 3.4 SPAPB1E7.03 |rpc82||DNA-directed RNA polymerase III complex subu... 27 3.4 SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 5.9 SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Sc... 26 5.9 SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schi... 25 7.8 SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizo... 25 7.8 >SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 784 Score = 61.7 bits (143), Expect = 1e-10 Identities = 33/75 (44%), Positives = 48/75 (64%), Gaps = 1/75 (1%) Frame = +1 Query: 253 QSLLALKECIRAL-GMKGNNHLPFRVSKLTQVLRDSFIGDKSRTCMIAMISPAMASCEHS 429 +SLLAL CI +L + H+P+R SKLT++L+ S +G RTCMI ISP+ + + Sbjct: 318 RSLLALGNCINSLCEPRRRQHVPYRDSKLTRLLKFS-LGGNCRTCMIVCISPSSEHYDET 376 Query: 430 LNTLRYADRVKELGT 474 NTL+Y +R K + T Sbjct: 377 HNTLKYGNRAKNIKT 391 Score = 44.0 bits (99), Expect = 2e-05 Identities = 30/76 (39%), Positives = 39/76 (51%), Gaps = 13/76 (17%) Frame = +2 Query: 65 TSGQTSANSNSSRSHAVFQIVV-------------RSPGMHRVHGKFSLIDLAGNERGAD 205 T T AN+ SSRSHAV QI + S H+V FS IDLAG+ER + Sbjct: 243 TMSPTEANAVSSRSHAVLQIYITQTPKSGEKQEESESQNSHKVRSVFSFIDLAGSERASA 302 Query: 206 TSSANRQTRMESAEIN 253 T + ++ +E A IN Sbjct: 303 TKNRGKRL-VEGANIN 317 >SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharomyces pombe|chr 2|||Manual Length = 883 Score = 60.1 bits (139), Expect = 3e-10 Identities = 34/75 (45%), Positives = 47/75 (62%), Gaps = 1/75 (1%) Frame = +1 Query: 253 QSLLALKECIRAL-GMKGNNHLPFRVSKLTQVLRDSFIGDKSRTCMIAMISPAMASCEHS 429 +SLLAL CI AL H+P+R SKLT++L+ S +G RT MI +SP+ E + Sbjct: 319 KSLLALGNCINALCDPHRRAHVPYRDSKLTRLLKFS-LGGNCRTVMIVCVSPSSVHYEET 377 Query: 430 LNTLRYADRVKELGT 474 NTL+YA+R K + T Sbjct: 378 HNTLKYANRAKNIKT 392 Score = 42.7 bits (96), Expect = 5e-05 Identities = 30/70 (42%), Positives = 42/70 (60%), Gaps = 7/70 (10%) Frame = +2 Query: 65 TSGQTSANSNSSRSHAVFQIVV----RSPGMHRVH--GKFSLIDLAGNERGADTSSANRQ 226 T T AN+ SSRSHAV Q+ + R+ G++ H S+IDLAG+ER T++ R Sbjct: 251 TMSPTEANAASSRSHAVLQVTLIQKPRTAGINEDHTLATLSIIDLAGSERA--TATKLRG 308 Query: 227 TRM-ESAEIN 253 +R+ E A IN Sbjct: 309 SRLFEGANIN 318 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 59.3 bits (137), Expect = 5e-10 Identities = 32/72 (44%), Positives = 47/72 (65%) Frame = +1 Query: 253 QSLLALKECIRALGMKGNNHLPFRVSKLTQVLRDSFIGDKSRTCMIAMISPAMASCEHSL 432 QSLL L I AL K + H+P+R SKLT++L+DS +G K++T MI +S + E ++ Sbjct: 352 QSLLTLGRVINALVEKAH-HIPYRESKLTRLLQDS-LGGKTKTSMIVTVSSTNTNLEETI 409 Query: 433 NTLRYADRVKEL 468 +TL YA R K + Sbjct: 410 STLEYAARAKSI 421 Score = 23.0 bits (47), Expect(2) = 1.5 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +2 Query: 77 TSANSNSSRSHAVFQIVV 130 T N SSRSH++F I + Sbjct: 276 TKCNDLSSRSHSIFTITL 293 Score = 23.0 bits (47), Expect(2) = 1.5 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +2 Query: 161 KFSLIDLAGNERGADTSSANRQTRMESAEIN 253 K ++DLAG+E + + N++ R E+ IN Sbjct: 322 KLHMVDLAGSENIGRSGAENKRAR-ETGMIN 351 >SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 554 Score = 50.8 bits (116), Expect = 2e-07 Identities = 34/110 (30%), Positives = 55/110 (50%) Frame = +1 Query: 139 GHAPRAREVLAHRPGGQRARRRHLLRQPTDQNGKRGDQQSLLALKECIRALGMKGNNHLP 318 G R R L G + + + Q ++ K +SL L I +L +H+P Sbjct: 219 GETRRGRLFLVDLAGSESVGKSGAVGQTLEEAKKIN--RSLSTLGMVINSLTDSKLSHVP 276 Query: 319 FRVSKLTQVLRDSFIGDKSRTCMIAMISPAMASCEHSLNTLRYADRVKEL 468 +R SKLT++L++S +G SRT +I SP + +L+TLR+ R K + Sbjct: 277 YRDSKLTRILKES-LGGNSRTTLIINCSPDSYNATETLSTLRFGHRAKSI 325 Score = 36.3 bits (80), Expect = 0.004 Identities = 26/63 (41%), Positives = 35/63 (55%), Gaps = 4/63 (6%) Frame = +2 Query: 77 TSANSNSSRSHAVFQIVV----RSPGMHRVHGKFSLIDLAGNERGADTSSANRQTRMESA 244 TS N+ SSRSH++F + V G R G+ L+DLAG+E S A QT E+ Sbjct: 194 TSMNAQSSRSHSIFVLEVVQTDTESGETR-RGRLFLVDLAGSE-SVGKSGAVGQTLEEAK 251 Query: 245 EIN 253 +IN Sbjct: 252 KIN 254 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 46.4 bits (105), Expect = 4e-06 Identities = 28/77 (36%), Positives = 45/77 (58%), Gaps = 6/77 (7%) Frame = +1 Query: 253 QSLLALKECIRALGMKGNN------HLPFRVSKLTQVLRDSFIGDKSRTCMIAMISPAMA 414 +SL L + I ALG N+ H+P+R SKLT +L+ S +G ++T M +SP + Sbjct: 746 KSLSCLGDVIHALGNASNSTTKEKSHIPYRNSKLTYLLKYS-LGKGAKTLMFVNVSPLKS 804 Query: 415 SCEHSLNTLRYADRVKE 465 +LN+LR+A +V + Sbjct: 805 QFMDTLNSLRFATKVND 821 Score = 35.9 bits (79), Expect = 0.006 Identities = 23/62 (37%), Positives = 33/62 (53%), Gaps = 3/62 (4%) Frame = +2 Query: 77 TSANSNSSRSHAVFQIVV---RSPGMHRVHGKFSLIDLAGNERGADTSSANRQTRMESAE 247 T AN SSRSH VF + + S G +L+DLAG+ER + + + + R E+ Sbjct: 685 TKANERSSRSHTVFMLYIDGENSRTKQICKGTLNLVDLAGSERLSYSQAVGDRLR-ETQA 743 Query: 248 IN 253 IN Sbjct: 744 IN 745 >SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 46.0 bits (104), Expect = 5e-06 Identities = 30/77 (38%), Positives = 45/77 (58%), Gaps = 3/77 (3%) Frame = +1 Query: 253 QSLLALKECIRALGM-KGNNHLPFRVSKLTQVLRDSFIGDKSRTCMIAMISPAMASCEHS 429 +SL L + I ALG K ++P+R SKLT +L+ S +G S+T M ISP + Sbjct: 736 KSLSCLGDVIHALGSGKEGTYIPYRNSKLTNLLQYS-LGGNSKTLMFVNISPLKQHVPET 794 Query: 430 LNTLRYADRVK--ELGT 474 L +LR+A +V ++GT Sbjct: 795 LCSLRFATKVNNTQIGT 811 Score = 36.3 bits (80), Expect = 0.004 Identities = 23/62 (37%), Positives = 35/62 (56%), Gaps = 3/62 (4%) Frame = +2 Query: 77 TSANSNSSRSHAVFQIVV---RSPGMHRVHGKFSLIDLAGNERGADTSSANRQTRMESAE 247 T+AN +SSRSH+VF + + S +LIDLAG+ER + + S + + E+ Sbjct: 675 TNANEHSSRSHSVFMLHLNGSNSTTGETCRSTLNLIDLAGSERLSSSQSVGERLK-ETQA 733 Query: 248 IN 253 IN Sbjct: 734 IN 735 >SPBC1604.20c |tea2|klp4|kinesin-like protein Tea2|Schizosaccharomyces pombe|chr 2|||Manual Length = 628 Score = 40.3 bits (90), Expect = 3e-04 Identities = 26/62 (41%), Positives = 36/62 (58%), Gaps = 3/62 (4%) Frame = +2 Query: 77 TSANSNSSRSHAVFQI--VVRSPGMHRVH-GKFSLIDLAGNERGADTSSANRQTRMESAE 247 T N+ SSRSHA+ Q+ + +P H SL+DLAG+ER +SA+ + R E A Sbjct: 325 TDFNTYSSRSHAILQVFLIRNNPTAHTSQISSLSLVDLAGSER----ASAHHERRKEGAF 380 Query: 248 IN 253 IN Sbjct: 381 IN 382 Score = 39.5 bits (88), Expect = 4e-04 Identities = 20/55 (36%), Positives = 34/55 (61%) Frame = +1 Query: 304 NNHLPFRVSKLTQVLRDSFIGDKSRTCMIAMISPAMASCEHSLNTLRYADRVKEL 468 + H+P+R SKLT++L+ S G +S+ ++A IS + NTL++A R + L Sbjct: 407 SGHIPYRESKLTRLLQQSLSG-QSQISLLATISIESNHTMETTNTLKFASRAQNL 460 >SPBC15D4.01c ||SPBC2D10.21c|kinesin-like protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 633 Score = 37.9 bits (84), Expect = 0.001 Identities = 25/76 (32%), Positives = 40/76 (52%), Gaps = 4/76 (5%) Frame = +1 Query: 253 QSLLALKECIRALGMK--GNNHL-PFRVSKLTQVL-RDSFIGDKSRTCMIAMISPAMASC 420 +SLL L +C+ AL K G H+ PFR SKLT++L + + M+ I P S Sbjct: 318 RSLLTLGQCLEALRRKHEGKQHIIPFRQSKLTELLFHSGHLSGLAGINMLVNIDP-FGSF 376 Query: 421 EHSLNTLRYADRVKEL 468 + + +RY+ +E+ Sbjct: 377 DENAQVMRYSANAREI 392 >SPBC651.09c |||RNA polymerase II associated Paf1 complex |Schizosaccharomyces pombe|chr 2|||Manual Length = 560 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/50 (22%), Positives = 25/50 (50%) Frame = +3 Query: 519 MLPARDDLAHLRSLNDGDMSAEMYTFHEAIDELQRAEEEVLDNHKAVSDY 668 M +D+L L ++D D+++ H+ +D++ + + +N DY Sbjct: 1 MADFQDELLALAGIDDSDVASNRKRAHDDLDDVLSSSSDEDNNENVGQDY 50 >SPCPB16A4.03c |ade10||IMP cyclohydrolase|Schizosaccharomyces pombe|chr 3|||Manual Length = 585 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +1 Query: 103 LARRVPDRGAVPGHAPRAREVLAHRPGGQ 189 ++R V D PG+ P A E+L + GG+ Sbjct: 325 ISREVSDGVIAPGYEPEALELLKKKKGGK 353 >SPAPB1E7.03 |rpc82||DNA-directed RNA polymerase III complex subunit Rpc82 |Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/41 (31%), Positives = 22/41 (53%) Frame = +3 Query: 528 ARDDLAHLRSLNDGDMSAEMYTFHEAIDELQRAEEEVLDNH 650 A ++L + LN+ D AE + E++DE + +VL H Sbjct: 349 ASNELRVMEKLNELDELAEEDNYEESVDENANRKSKVLAQH 389 >SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 215 Score = 25.8 bits (54), Expect = 5.9 Identities = 8/19 (42%), Positives = 11/19 (57%) Frame = -3 Query: 156 CTRCMPGDRTTIWNTACER 100 C C P +IW TAC++ Sbjct: 80 CRTCQPKQHDSIWCTACQQ 98 >SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/35 (37%), Positives = 16/35 (45%) Frame = -2 Query: 583 SADMSPSLRERRCARSSRAGSMSTSAAETRRAGTG 479 S SPSL + S AG ++ E R GTG Sbjct: 158 STPASPSLESHEFSESREAGLSQSNGFEARSHGTG 192 >SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 25.4 bits (53), Expect = 7.8 Identities = 14/65 (21%), Positives = 32/65 (49%) Frame = +2 Query: 65 TSGQTSANSNSSRSHAVFQIVVRSPGMHRVHGKFSLIDLAGNERGADTSSANRQTRMESA 244 TSG+ SA S+++ + + V+ ++H K SL ++ + ++ ++R T+ Sbjct: 325 TSGEVSAQSSTNSTSSEESTSVKDEDSSKIHDK-SLKRKHEDDESSTSTKSSRTTKTPFT 383 Query: 245 EINSP 259 + P Sbjct: 384 RVGDP 388 >SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizosaccharomyces pombe|chr 2|||Manual Length = 589 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/53 (28%), Positives = 24/53 (45%) Frame = -1 Query: 545 RQVVPRGQHVHVGSGDSPRRDGSMVPSSFTLSAYRSVLRLCSQEAMAGEIMAI 387 R ++ G H +G + R DG +PSS L Y +L + A + A+ Sbjct: 41 RNILLYGSCKHSENGCAFRHDGPFIPSSENLEQYSVKKKLNAASASFQPVRAL 93 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,165,568 Number of Sequences: 5004 Number of extensions: 38886 Number of successful extensions: 162 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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