BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021108 (851 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 99 3e-21 At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 97 2e-20 At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 94 9e-20 At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 94 9e-20 At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 94 9e-20 At5g47850.1 68418.m05912 protein kinase, putative contains simil... 29 5.2 >At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Catharanthus roseus] SWISS-PROT:Q96552 Length = 393 Score = 99.1 bits (236), Expect = 3e-21 Identities = 48/96 (50%), Positives = 64/96 (66%) Frame = +1 Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684 ++PLT VLA KL K+ E+R+NG W RPD KTQVT EY+ GA VP RVHTV++S Q Sbjct: 137 LMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAMVPVRVHTVLISTQ 196 Query: 685 HSEKITLETLRDEIREKVIKEVIPAQILMREL*FTL 792 H E +T + + +++E VIK VIP + L + F L Sbjct: 197 HDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHL 232 Score = 97.9 bits (233), Expect = 7e-21 Identities = 45/82 (54%), Positives = 62/82 (75%), Gaps = 2/82 (2%) Frame = +2 Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433 V++ GEIT+KANVDY+++VR+T + IG+ + G D C V++ ++QQSP+IA GVH + Sbjct: 51 VMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGH 110 Query: 434 --RNDEEVGAGDQGLMFGYATD 493 + EEVGAGDQG MFGYATD Sbjct: 111 LTKKPEEVGAGDQGHMFGYATD 132 Score = 88.2 bits (209), Expect = 6e-18 Identities = 43/70 (61%), Positives = 50/70 (71%), Gaps = 4/70 (5%) Frame = +3 Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCF----CVAKSHP 281 FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT M K++ Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63 Query: 282 KLTWIIKKLC 311 I++K C Sbjct: 64 DYEQIVRKTC 73 Score = 52.0 bits (119), Expect = 5e-07 Identities = 20/29 (68%), Positives = 24/29 (82%) Frame = +3 Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851 LDE+T+ H+NP G F+IGGP DAGLTGR Sbjct: 224 LDEKTIFHLNPSGRFVIGGPHGDAGLTGR 252 >At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 3 (Methionine adenosyltransferase 3, AdoMet synthetase 3) [Lycopersicon esculentum] SWISS-PROT:P43282 Length = 390 Score = 96.7 bits (230), Expect = 2e-20 Identities = 46/88 (52%), Positives = 61/88 (69%) Frame = +1 Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684 ++PLT VLA KL K+ E+R+N W RPD KTQVT EY GGA +P RVHTV++S Q Sbjct: 137 LMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAMIPIRVHTVLISTQ 196 Query: 685 HSEKITLETLRDEIREKVIKEVIPAQIL 768 H E +T + + +++E VIK VIPA+ L Sbjct: 197 HDETVTNDEIAADLKEHVIKPVIPAKYL 224 Score = 92.7 bits (220), Expect = 3e-19 Identities = 42/82 (51%), Positives = 60/82 (73%), Gaps = 2/82 (2%) Frame = +2 Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433 V++ GEIT+ A VDY+K+VR T + IG+ + G D C+V++ ++QQSP+IA GVH + Sbjct: 51 VMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGH 110 Query: 434 --RNDEEVGAGDQGLMFGYATD 493 + E++GAGDQG MFGYATD Sbjct: 111 LTKKPEDIGAGDQGHMFGYATD 132 Score = 87.0 bits (206), Expect = 1e-17 Identities = 39/47 (82%), Positives = 42/47 (89%) Frame = +3 Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGM 254 FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT M Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNM 50 Score = 51.6 bits (118), Expect = 6e-07 Identities = 20/33 (60%), Positives = 24/33 (72%) Frame = +3 Query: 753 PCTNLDERTVIHINPCGLFIIGGPQSDAGLTGR 851 P LD+ T+ H+NP G F+IGGP DAGLTGR Sbjct: 220 PAKYLDDNTIFHLNPSGRFVIGGPHGDAGLTGR 252 >At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2) identical to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Arabidopsis thaliana] SWISS-PROT:P17562 Length = 393 Score = 94.3 bits (224), Expect = 9e-20 Identities = 42/82 (51%), Positives = 60/82 (73%), Gaps = 2/82 (2%) Frame = +2 Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433 V++ GEIT+KA +DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + Sbjct: 51 VMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110 Query: 434 --RNDEEVGAGDQGLMFGYATD 493 + E++GAGDQG MFGYATD Sbjct: 111 FTKRPEDIGAGDQGHMFGYATD 132 Score = 93.1 bits (221), Expect = 2e-19 Identities = 42/88 (47%), Positives = 60/88 (68%) Frame = +1 Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684 ++PL+ VLA K+ ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S Q Sbjct: 137 LMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQ 196 Query: 685 HSEKITLETLRDEIREKVIKEVIPAQIL 768 H E +T + + +++E VIK +IP + L Sbjct: 197 HDETVTNDEIARDLKEHVIKPIIPEKYL 224 Score = 89.4 bits (212), Expect = 3e-18 Identities = 42/65 (64%), Positives = 48/65 (73%) Frame = +3 Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M + K T Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63 Query: 294 IIKKL 308 +K+ Sbjct: 64 DYEKI 68 Score = 50.8 bits (116), Expect = 1e-06 Identities = 19/29 (65%), Positives = 24/29 (82%) Frame = +3 Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851 LD++T+ H+NP G F+IGGP DAGLTGR Sbjct: 224 LDDKTIFHLNPSGRFVIGGPHGDAGLTGR 252 >At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 94.3 bits (224), Expect = 9e-20 Identities = 44/82 (53%), Positives = 60/82 (73%), Gaps = 2/82 (2%) Frame = +2 Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433 V++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + Sbjct: 51 VMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110 Query: 434 --RNDEEVGAGDQGLMFGYATD 493 + EE+GAGDQG MFGYATD Sbjct: 111 FTKCPEEIGAGDQGHMFGYATD 132 Score = 93.5 bits (222), Expect = 2e-19 Identities = 46/96 (47%), Positives = 63/96 (65%) Frame = +1 Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684 ++PL+ VLA KL ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S Q Sbjct: 137 LMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQ 196 Query: 685 HSEKITLETLRDEIREKVIKEVIPAQILMREL*FTL 792 H E +T + + +++E VIK VIP + L + F L Sbjct: 197 HDETVTNDEIARDLKEHVIKPVIPEKYLDEKTIFHL 232 Score = 89.4 bits (212), Expect = 3e-18 Identities = 42/65 (64%), Positives = 48/65 (73%) Frame = +3 Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M + K T Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63 Query: 294 IIKKL 308 +K+ Sbjct: 64 DYEKI 68 Score = 52.0 bits (119), Expect = 5e-07 Identities = 20/29 (68%), Positives = 24/29 (82%) Frame = +3 Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851 LDE+T+ H+NP G F+IGGP DAGLTGR Sbjct: 224 LDEKTIFHLNPSGRFVIGGPHGDAGLTGR 252 >At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 94.3 bits (224), Expect = 9e-20 Identities = 44/82 (53%), Positives = 60/82 (73%), Gaps = 2/82 (2%) Frame = +2 Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433 V++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + Sbjct: 51 VMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110 Query: 434 --RNDEEVGAGDQGLMFGYATD 493 + EE+GAGDQG MFGYATD Sbjct: 111 FTKCPEEIGAGDQGHMFGYATD 132 Score = 93.5 bits (222), Expect = 2e-19 Identities = 46/96 (47%), Positives = 63/96 (65%) Frame = +1 Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684 ++PL+ VLA KL ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S Q Sbjct: 137 LMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQ 196 Query: 685 HSEKITLETLRDEIREKVIKEVIPAQILMREL*FTL 792 H E +T + + +++E VIK VIP + L + F L Sbjct: 197 HDETVTNDEIARDLKEHVIKPVIPEKYLDEKTIFHL 232 Score = 89.4 bits (212), Expect = 3e-18 Identities = 42/65 (64%), Positives = 48/65 (73%) Frame = +3 Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M + K T Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63 Query: 294 IIKKL 308 +K+ Sbjct: 64 DYEKI 68 Score = 52.0 bits (119), Expect = 5e-07 Identities = 20/29 (68%), Positives = 24/29 (82%) Frame = +3 Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851 LDE+T+ H+NP G F+IGGP DAGLTGR Sbjct: 224 LDEKTIFHLNPSGRFVIGGPHGDAGLTGR 252 >At5g47850.1 68418.m05912 protein kinase, putative contains similarity to cytokinin-regulated kinase 1 [Nicotiana tabacum] gi|10998537|gb|AAG25966; contains protein kinase domain, Pfam:PF00069 Length = 751 Score = 28.7 bits (61), Expect = 5.2 Identities = 19/57 (33%), Positives = 26/57 (45%), Gaps = 3/57 (5%) Frame = -1 Query: 824 RSSNYKESTWINVNYSSLIKICAGITSLM---TFSLISSRKVSRVIFSECCSDTTTV 663 R+ N K TW N + L+ C G SL+ +F + S RV S DT T+ Sbjct: 351 RAQNSKNKTWSRRNIAFLVVGCVGTFSLLLVISFLIFKSHCRCRVHDSGRLDDTRTI 407 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,087,888 Number of Sequences: 28952 Number of extensions: 452379 Number of successful extensions: 1277 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 1214 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1272 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1980143200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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