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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021108
         (851 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati...    99   3e-21
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati...    97   2e-20
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2...    94   9e-20
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1...    94   9e-20
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1...    94   9e-20
At5g47850.1 68418.m05912 protein kinase, putative contains simil...    29   5.2  

>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 2 (Methionine
           adenosyltransferase 2, AdoMet synthetase 2)
           [Catharanthus roseus] SWISS-PROT:Q96552
          Length = 393

 Score = 99.1 bits (236), Expect = 3e-21
 Identities = 48/96 (50%), Positives = 64/96 (66%)
 Frame = +1

Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684
           ++PLT VLA KL  K+ E+R+NG   W RPD KTQVT EY+   GA VP RVHTV++S Q
Sbjct: 137 LMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAMVPVRVHTVLISTQ 196

Query: 685 HSEKITLETLRDEIREKVIKEVIPAQILMREL*FTL 792
           H E +T + +  +++E VIK VIP + L  +  F L
Sbjct: 197 HDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHL 232



 Score = 97.9 bits (233), Expect = 7e-21
 Identities = 45/82 (54%), Positives = 62/82 (75%), Gaps = 2/82 (2%)
 Frame = +2

Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
           V++ GEIT+KANVDY+++VR+T + IG+  +  G D   C V++ ++QQSP+IA GVH +
Sbjct: 51  VMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGH 110

Query: 434 --RNDEEVGAGDQGLMFGYATD 493
             +  EEVGAGDQG MFGYATD
Sbjct: 111 LTKKPEEVGAGDQGHMFGYATD 132



 Score = 88.2 bits (209), Expect = 6e-18
 Identities = 43/70 (61%), Positives = 50/70 (71%), Gaps = 4/70 (5%)
 Frame = +3

Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCF----CVAKSHP 281
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT M         K++ 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63

Query: 282 KLTWIIKKLC 311
               I++K C
Sbjct: 64  DYEQIVRKTC 73



 Score = 52.0 bits (119), Expect = 5e-07
 Identities = 20/29 (68%), Positives = 24/29 (82%)
 Frame = +3

Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851
           LDE+T+ H+NP G F+IGGP  DAGLTGR
Sbjct: 224 LDEKTIFHLNPSGRFVIGGPHGDAGLTGR 252


>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 3 (Methionine
           adenosyltransferase 3, AdoMet synthetase 3)
           [Lycopersicon esculentum] SWISS-PROT:P43282
          Length = 390

 Score = 96.7 bits (230), Expect = 2e-20
 Identities = 46/88 (52%), Positives = 61/88 (69%)
 Frame = +1

Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684
           ++PLT VLA KL  K+ E+R+N    W RPD KTQVT EY   GGA +P RVHTV++S Q
Sbjct: 137 LMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAMIPIRVHTVLISTQ 196

Query: 685 HSEKITLETLRDEIREKVIKEVIPAQIL 768
           H E +T + +  +++E VIK VIPA+ L
Sbjct: 197 HDETVTNDEIAADLKEHVIKPVIPAKYL 224



 Score = 92.7 bits (220), Expect = 3e-19
 Identities = 42/82 (51%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
 Frame = +2

Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
           V++ GEIT+ A VDY+K+VR T + IG+  +  G D   C+V++ ++QQSP+IA GVH +
Sbjct: 51  VMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGH 110

Query: 434 --RNDEEVGAGDQGLMFGYATD 493
             +  E++GAGDQG MFGYATD
Sbjct: 111 LTKKPEDIGAGDQGHMFGYATD 132



 Score = 87.0 bits (206), Expect = 1e-17
 Identities = 39/47 (82%), Positives = 42/47 (89%)
 Frame = +3

Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGM 254
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT M
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNM 50



 Score = 51.6 bits (118), Expect = 6e-07
 Identities = 20/33 (60%), Positives = 24/33 (72%)
 Frame = +3

Query: 753 PCTNLDERTVIHINPCGLFIIGGPQSDAGLTGR 851
           P   LD+ T+ H+NP G F+IGGP  DAGLTGR
Sbjct: 220 PAKYLDDNTIFHLNPSGRFVIGGPHGDAGLTGR 252


>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
           identical to S-adenosylmethionine synthetase 2
           (Methionine adenosyltransferase 2, AdoMet synthetase 2)
           [Arabidopsis thaliana] SWISS-PROT:P17562
          Length = 393

 Score = 94.3 bits (224), Expect = 9e-20
 Identities = 42/82 (51%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
 Frame = +2

Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
           V++ GEIT+KA +DY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +
Sbjct: 51  VMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110

Query: 434 --RNDEEVGAGDQGLMFGYATD 493
             +  E++GAGDQG MFGYATD
Sbjct: 111 FTKRPEDIGAGDQGHMFGYATD 132



 Score = 93.1 bits (221), Expect = 2e-19
 Identities = 42/88 (47%), Positives = 60/88 (68%)
 Frame = +1

Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684
           ++PL+ VLA K+  ++ E+R+NG   W RPD KTQVT EY    GA VP RVHTV++S Q
Sbjct: 137 LMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQ 196

Query: 685 HSEKITLETLRDEIREKVIKEVIPAQIL 768
           H E +T + +  +++E VIK +IP + L
Sbjct: 197 HDETVTNDEIARDLKEHVIKPIIPEKYL 224



 Score = 89.4 bits (212), Expect = 3e-18
 Identities = 42/65 (64%), Positives = 48/65 (73%)
 Frame = +3

Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M     +   K T 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63

Query: 294 IIKKL 308
             +K+
Sbjct: 64  DYEKI 68



 Score = 50.8 bits (116), Expect = 1e-06
 Identities = 19/29 (65%), Positives = 24/29 (82%)
 Frame = +3

Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851
           LD++T+ H+NP G F+IGGP  DAGLTGR
Sbjct: 224 LDDKTIFHLNPSGRFVIGGPHGDAGLTGR 252


>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score = 94.3 bits (224), Expect = 9e-20
 Identities = 44/82 (53%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
 Frame = +2

Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
           V++ GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +
Sbjct: 51  VMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110

Query: 434 --RNDEEVGAGDQGLMFGYATD 493
             +  EE+GAGDQG MFGYATD
Sbjct: 111 FTKCPEEIGAGDQGHMFGYATD 132



 Score = 93.5 bits (222), Expect = 2e-19
 Identities = 46/96 (47%), Positives = 63/96 (65%)
 Frame = +1

Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684
           ++PL+ VLA KL  ++ E+R+NG   W RPD KTQVT EY    GA VP RVHTV++S Q
Sbjct: 137 LMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQ 196

Query: 685 HSEKITLETLRDEIREKVIKEVIPAQILMREL*FTL 792
           H E +T + +  +++E VIK VIP + L  +  F L
Sbjct: 197 HDETVTNDEIARDLKEHVIKPVIPEKYLDEKTIFHL 232



 Score = 89.4 bits (212), Expect = 3e-18
 Identities = 42/65 (64%), Positives = 48/65 (73%)
 Frame = +3

Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M     +   K T 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63

Query: 294 IIKKL 308
             +K+
Sbjct: 64  DYEKI 68



 Score = 52.0 bits (119), Expect = 5e-07
 Identities = 20/29 (68%), Positives = 24/29 (82%)
 Frame = +3

Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851
           LDE+T+ H+NP G F+IGGP  DAGLTGR
Sbjct: 224 LDEKTIFHLNPSGRFVIGGPHGDAGLTGR 252


>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score = 94.3 bits (224), Expect = 9e-20
 Identities = 44/82 (53%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
 Frame = +2

Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
           V++ GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +
Sbjct: 51  VMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110

Query: 434 --RNDEEVGAGDQGLMFGYATD 493
             +  EE+GAGDQG MFGYATD
Sbjct: 111 FTKCPEEIGAGDQGHMFGYATD 132



 Score = 93.5 bits (222), Expect = 2e-19
 Identities = 46/96 (47%), Positives = 63/96 (65%)
 Frame = +1

Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQ 684
           ++PL+ VLA KL  ++ E+R+NG   W RPD KTQVT EY    GA VP RVHTV++S Q
Sbjct: 137 LMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQ 196

Query: 685 HSEKITLETLRDEIREKVIKEVIPAQILMREL*FTL 792
           H E +T + +  +++E VIK VIP + L  +  F L
Sbjct: 197 HDETVTNDEIARDLKEHVIKPVIPEKYLDEKTIFHL 232



 Score = 89.4 bits (212), Expect = 3e-18
 Identities = 42/65 (64%), Positives = 48/65 (73%)
 Frame = +3

Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M     +   K T 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63

Query: 294 IIKKL 308
             +K+
Sbjct: 64  DYEKI 68



 Score = 52.0 bits (119), Expect = 5e-07
 Identities = 20/29 (68%), Positives = 24/29 (82%)
 Frame = +3

Query: 765 LDERTVIHINPCGLFIIGGPQSDAGLTGR 851
           LDE+T+ H+NP G F+IGGP  DAGLTGR
Sbjct: 224 LDEKTIFHLNPSGRFVIGGPHGDAGLTGR 252


>At5g47850.1 68418.m05912 protein kinase, putative contains
           similarity to cytokinin-regulated kinase 1 [Nicotiana
           tabacum] gi|10998537|gb|AAG25966; contains protein
           kinase domain, Pfam:PF00069
          Length = 751

 Score = 28.7 bits (61), Expect = 5.2
 Identities = 19/57 (33%), Positives = 26/57 (45%), Gaps = 3/57 (5%)
 Frame = -1

Query: 824 RSSNYKESTWINVNYSSLIKICAGITSLM---TFSLISSRKVSRVIFSECCSDTTTV 663
           R+ N K  TW   N + L+  C G  SL+   +F +  S    RV  S    DT T+
Sbjct: 351 RAQNSKNKTWSRRNIAFLVVGCVGTFSLLLVISFLIFKSHCRCRVHDSGRLDDTRTI 407


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,087,888
Number of Sequences: 28952
Number of extensions: 452379
Number of successful extensions: 1277
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1272
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1980143200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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