BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021051 (768 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 29 0.97 SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imida... 29 0.97 SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 27 3.0 SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 27 3.0 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.8 SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 25 9.0 SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5 |Schizosacch... 25 9.0 >SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosaccharomyces pombe|chr 3|||Manual Length = 1213 Score = 28.7 bits (61), Expect = 0.97 Identities = 16/57 (28%), Positives = 27/57 (47%) Frame = +1 Query: 505 PTPYDLSRFASSHLKPLQIHVCNDYSAFDQSQHGEAVVLERKKMERLSIPSHLIDLH 675 P +D + SS+ + IH N++ S+H E L K +S+P ++LH Sbjct: 192 PEKWDTKKVKSSNRNFVTIHELNEHLKNVNSKHSEIDDLLPDKRSIVSLPPSTLNLH 248 >SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imidazole acetol phosphate transaminase His3|Schizosaccharomyces pombe|chr 2|||Manual Length = 384 Score = 28.7 bits (61), Expect = 0.97 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +1 Query: 172 INDIPIIKFLFSPTFSLNVSISMSTHS 252 IND+ ++K L P F+LNV T S Sbjct: 124 INDVEVVKVLLEPDFNLNVDAICETLS 150 >SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 27.1 bits (57), Expect = 3.0 Identities = 14/53 (26%), Positives = 24/53 (45%) Frame = -3 Query: 610 PHHAEIGQTQNSHCRHEFVMVSDGTKQTERDRMELASIHKQVRRTIFTVENSL 452 P+H ++GQ S E + +Q E+ + EL + +F VEN + Sbjct: 446 PYHLQVGQAWTSESERE---LEKKKEQVEKKQEELMQTRIVLEEQVFLVENMI 495 >SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 982 Score = 27.1 bits (57), Expect = 3.0 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = -1 Query: 165 DIEMKTEMCQGLRQKEKVCKEGRRVGNGVFWQWRPKVK 52 ++E + QG Q+ K KEG + + QWR VK Sbjct: 905 ELEAEDSGAQGDTQEVKSKKEGMAIVRDIMRQWRSNVK 942 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 25.8 bits (54), Expect = 6.8 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +1 Query: 85 VSDPPSFLTDFLLLTKSLAHFCFHL 159 + D P+ L F LL+K ++HF F L Sbjct: 309 IVDQPANLLKFSLLSKKVSHFLFTL 333 >SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase Ade3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 25.4 bits (53), Expect = 9.0 Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 6/68 (8%) Frame = -3 Query: 661 DEMEWKAAPFSSVPK---QRPHHAEI---GQTQNSHCRHEFVMVSDGTKQTERDRMELAS 500 DE+++ ++S P + P E+ GQ + HCRH+ + +D T E+ S Sbjct: 188 DEIDYLVECYTSEPSLKSREPTDVELFMFGQVNSEHCRHK-IFNADWTIDGEKKDY---S 243 Query: 499 IHKQVRRT 476 + K +R T Sbjct: 244 LFKMIRNT 251 >SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 834 Score = 25.4 bits (53), Expect = 9.0 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +3 Query: 111 RLSPSDEVLGTFLFSSLCRAYKRHPNHKVPFQPDLF 218 RL D + S +C +R P + + F PDLF Sbjct: 180 RLDDPDTSVVNAAVSVICELARRAPKNYLEFAPDLF 215 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,466,700 Number of Sequences: 5004 Number of extensions: 77357 Number of successful extensions: 260 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 248 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 260 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 369323696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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