BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021051 (768 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g19020.1 68418.m02260 pentatricopeptide (PPR) repeat-containi... 31 0.64 At1g04700.1 68414.m00467 protein kinase family protein low simil... 31 0.64 At5g01940.1 68418.m00113 eukaryotic translation initiation facto... 31 0.85 At3g08850.1 68416.m01029 transducin family protein / WD-40 repea... 30 1.5 At1g66235.1 68414.m07518 expressed protein ; expression supporte... 29 3.4 At4g12940.1 68417.m02023 expressed protein hypothetical protein ... 29 4.5 At3g06180.1 68416.m00710 expressed protein 29 4.5 At1g08470.1 68414.m00938 strictosidine synthase family protein s... 28 6.0 At1g04590.2 68414.m00453 expressed protein isoform contains GG a... 28 6.0 At1g04590.1 68414.m00452 expressed protein isoform contains GG a... 28 6.0 At5g08540.1 68418.m01016 expressed protein similar to unknown pr... 28 7.9 At4g27010.1 68417.m03885 expressed protein ; expression support... 28 7.9 At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 28 7.9 At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein si... 28 7.9 At1g31630.1 68414.m03884 MADS-box family protein similar to MADS... 28 7.9 >At5g19020.1 68418.m02260 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 939 Score = 31.5 bits (68), Expect = 0.64 Identities = 25/77 (32%), Positives = 34/77 (44%) Frame = +1 Query: 79 DSVSDPPSFLTDFLLLTKSLAHFCFHLYVEPINDIPIIKFLFSPTFSLNVSISMSTHSFQ 258 +S + PPS + + S H C H ++ + +I LFS TF L+ S HS Sbjct: 20 NSTTMPPSSRSAANQNSSSSLHLCKHSPSATLDLLILILVLFSGTFLLSSYFSYLIHSLS 79 Query: 259 QDSSCHPSERKSFGSRL 309 SS PS S S L Sbjct: 80 LLSSHFPSITISLSSLL 96 >At1g04700.1 68414.m00467 protein kinase family protein low similarity to EDR1 [Arabidopsis thaliana] GI:11127925; contains Pfam profile: PF00069 Eukaryotic protein kinase domain Length = 1042 Score = 31.5 bits (68), Expect = 0.64 Identities = 17/42 (40%), Positives = 24/42 (57%) Frame = +1 Query: 202 FSPTFSLNVSISMSTHSFQQDSSCHPSERKSFGSRLAVHCCP 327 FSPTF L S + S H+++ S P+ K +G+ AVH P Sbjct: 289 FSPTFHLRDSPT-SVHTWEHKDSNSPTFMKPYGNTNAVHFMP 329 >At5g01940.1 68418.m00113 eukaryotic translation initiation factor 2B family protein / eIF-2B family protein similar to SP|P41035 Eukaryotic translation initiation factor 2 subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains Pfam profile PF01873: Domain found in IF2B/IF5 Length = 231 Score = 31.1 bits (67), Expect = 0.85 Identities = 20/71 (28%), Positives = 32/71 (45%) Frame = +3 Query: 84 RFRPSFLPYRLSPSDEVLGTFLFSSLCRAYKRHPNHKVPFQPDLFVECINLNEYSQLPTR 263 R R +P +L + F+ LCR R P+H + F ++LN+ +L + Sbjct: 97 RPRTVMMPPQLLAEGTITVCLNFADLCRTMHRKPDHVMKFLLAQMETKVSLNKQQRLEIK 156 Query: 264 LKLSSKRTQIV 296 +SSK Q V Sbjct: 157 GLVSSKDFQAV 167 >At3g08850.1 68416.m01029 transducin family protein / WD-40 repeat family protein similar to WD-repeat protein mip1 (SP:P87141) [Schizosaccharomyces pombe]; contains Pfam PF00400: WD domain, G-beta repeat (5 copies, 1 weak) Length = 1344 Score = 30.3 bits (65), Expect = 1.5 Identities = 15/30 (50%), Positives = 20/30 (66%), Gaps = 2/30 (6%) Frame = -3 Query: 313 PPVGIRTICV--RLDDSLSLVGSCEYSLRL 230 P GI +C+ LDDSL LV SC+ S+R+ Sbjct: 1071 PDKGISKLCLINELDDSLLLVASCDGSVRI 1100 >At1g66235.1 68414.m07518 expressed protein ; expression supported by MPSS Length = 265 Score = 29.1 bits (62), Expect = 3.4 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = +3 Query: 615 CFGTEENGAAFHSISSYRPPLLHQNQHYKSIWTFD 719 C +GA+ I + +L Q++H+KS W FD Sbjct: 63 CENRRSSGASSDDIFNQAKEMLMQDKHFKSGWKFD 97 >At4g12940.1 68417.m02023 expressed protein hypothetical protein - Arabidopsis thaliana,PIR2:T08948; expression supported by MPSS Length = 151 Score = 28.7 bits (61), Expect = 4.5 Identities = 17/54 (31%), Positives = 28/54 (51%) Frame = +3 Query: 54 LLSASIAKRLRFRPSFLPYRLSPSDEVLGTFLFSSLCRAYKRHPNHKVPFQPDL 215 L+ +SIAK+ +P + S+ +F F + ++R N K PF+PDL Sbjct: 17 LIESSIAKQ---KPDLDHVNVDMSEIDYSSF-FKQMIEGWEREANEKYPFRPDL 66 >At3g06180.1 68416.m00710 expressed protein Length = 241 Score = 28.7 bits (61), Expect = 4.5 Identities = 22/70 (31%), Positives = 29/70 (41%) Frame = +1 Query: 73 PKDSVSDPPSFLTDFLLLTKSLAHFCFHLYVEPINDIPIIKFLFSPTFSLNVSISMSTHS 252 P S S S + + S H C H ++ + +I LFS F L+ S HS Sbjct: 20 PSSSASRSASNHSSSSSSSSSSLHLCKHSPSATLDLLILILVLFSGAFLLSSYFSYLFHS 79 Query: 253 FQQDSSCHPS 282 F SS PS Sbjct: 80 FSLLSSHFPS 89 >At1g08470.1 68414.m00938 strictosidine synthase family protein similar to strictosidine synthase [Rauvolfia serpentina][SP|P15324] Length = 390 Score = 28.3 bits (60), Expect = 6.0 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +2 Query: 590 TNLSMVRPLFWNGRKWSGFPFHLIL*TSIATSKPTL 697 T ++ R LFWNG +W+ F + + + KP+L Sbjct: 84 TGVADGRILFWNGTRWTDFAYTSNNRSELCDPKPSL 119 >At1g04590.2 68414.m00453 expressed protein isoform contains GG acceptor splice site at intron 7 Length = 384 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/55 (30%), Positives = 27/55 (49%) Frame = -2 Query: 536 EANRERSYGVGLHTQTSSEDDLYCRKLADIS*SGQKSRQWHHASRLMSGMLSKGR 372 E N+E YG L + E + L + S +K QWH +++ +LSKG+ Sbjct: 157 EDNKEAVYGA-LDAWVAWERNFPIASLKIVIASLEKEHQWHRMVQVIKWILSKGQ 210 >At1g04590.1 68414.m00452 expressed protein isoform contains GG acceptor splice site at intron 7 Length = 381 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/55 (30%), Positives = 27/55 (49%) Frame = -2 Query: 536 EANRERSYGVGLHTQTSSEDDLYCRKLADIS*SGQKSRQWHHASRLMSGMLSKGR 372 E N+E YG L + E + L + S +K QWH +++ +LSKG+ Sbjct: 157 EDNKEAVYGA-LDAWVAWERNFPIASLKIVIASLEKEHQWHRMVQVIKWILSKGQ 210 >At5g08540.1 68418.m01016 expressed protein similar to unknown protein (pir||T27191) Length = 346 Score = 27.9 bits (59), Expect = 7.9 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +3 Query: 144 FLFSSLCRAYKRHPNHKVPFQPDLFVECINLNEYSQL 254 F + R Y R+ ++ PF PDL + I+L + S L Sbjct: 186 FNMEEILRKYIRYALNEKPFNPDLVADLIHLRKASGL 222 >At4g27010.1 68417.m03885 expressed protein ; expression supported by MPSS Length = 2535 Score = 27.9 bits (59), Expect = 7.9 Identities = 9/18 (50%), Positives = 14/18 (77%) Frame = +1 Query: 151 FHLYVEPINDIPIIKFLF 204 F+LY +PIN++P+ F F Sbjct: 1927 FYLYDDPINEVPVFSFNF 1944 >At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4 other kinesin-like proteins of A. thaliana: F02P16.12 (PID:g2191180), katA (D11371), katB (D21137), and katC (D21138); contains non-consensus AT-AC splice sites at intron 10 Length = 1087 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +2 Query: 47 PDFTFGLHCQKTPFPTLLPSLQTFS 121 PD T L +TPFP +P +++FS Sbjct: 848 PDVTQNLPVSRTPFPARVPVVKSFS 872 >At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein similar to lysosomal alpha-mannosidase GI:3522867 from [Homo sapiens] Length = 1019 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = -3 Query: 310 PVGIRTICVRLDDSLSLVGSCEYSLRL 230 P+ IRT+ ++ DD + +VG E RL Sbjct: 993 PMEIRTLLIKFDDQIEMVGDKEQQHRL 1019 >At1g31630.1 68414.m03884 MADS-box family protein similar to MADS-box protein NMH 7 GI:2827300 from [Medicago sativa] Length = 339 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 183 PNHKVPFQPDLFVECINLNEYSQL 254 PNH+ PFQ D F+E + Y Q+ Sbjct: 267 PNHQYPFQDDPFMEMLMEYPYEQV 290 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,408,248 Number of Sequences: 28952 Number of extensions: 420377 Number of successful extensions: 1306 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 1253 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1306 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1721869952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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