BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021035
(812 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29B12.11c |||human WW domain binding protein-2 ortholog|Schi... 54 3e-08
SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizos... 29 0.79
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.4
SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy... 27 3.2
SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces po... 27 3.2
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 26 5.5
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 5.5
SPBC30B4.04c |sol1||SWI/SNF complex subunit Sol1|Schizosaccharom... 25 9.7
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 25 9.7
>SPAC29B12.11c |||human WW domain binding protein-2
ortholog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 53.6 bits (123), Expect = 3e-08
Identities = 26/77 (33%), Positives = 36/77 (46%)
Frame = +1
Query: 274 EGRMYLTTHRMIYNSKKNTDAMRSFSFPFIALQDVTVEQPMFSANCIKGKVRAQPNGNFI 453
+G + LT R++Y +K + F P L+D + QP F AN G V PNG
Sbjct: 49 KGLLCLTNQRLVYIAKDTDCDFKDFQSPVANLKDTKLNQPFFGANYYSGTVMPVPNGGIP 108
Query: 454 GEVKFKLTFKSGGAIEY 504
E + KL F GG +
Sbjct: 109 CEAEVKLQFNEGGIFNF 125
>SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 29.1 bits (62), Expect = 0.79
Identities = 23/74 (31%), Positives = 36/74 (48%), Gaps = 7/74 (9%)
Frame = +2
Query: 152 LNTAHADHGVLIHAGECII--LFSDNVSVEFYGNDTQ----SLRGSRRDGCTSLLIE*FT 313
LN A +D G I A + + L S N +EFYG+DT S+ +G TS + +T
Sbjct: 114 LNIAESDTGSSIEAQDSWVYQLNSFNKKIEFYGDDTWLKLFPSAFSKFEGTTSFFVSDYT 173
Query: 314 -IQRRIQMP*DHSV 352
+ + DH++
Sbjct: 174 EVDNNVTRNFDHAL 187
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 1.4
Identities = 18/37 (48%), Positives = 20/37 (54%), Gaps = 4/37 (10%)
Frame = +3
Query: 654 PPYSAFPD--QPPP--NSVFVSNTPPPYPGVTGASYP 752
PP SA QPPP +S VSN P P P + G S P
Sbjct: 365 PPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAP 401
>SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 802
Score = 27.1 bits (57), Expect = 3.2
Identities = 28/101 (27%), Positives = 45/101 (44%), Gaps = 1/101 (0%)
Frame = -3
Query: 756 RSDKKLQSRQGRE-VGYSTQILSLVEVGLEKQNKVAPIHNILVEEVHMLVVVRLATVQSV 580
R +K R+ RE V +L+ +++GLE H + E+ + LAT +
Sbjct: 371 RERRKANQRKQREIVRMQMGMLAPMDIGLE--------HEAMGEDS----LFGLATAEKH 418
Query: 579 GYKEVAKHQLPYDEKQDEQLLTWLSILNSTS*FEGKLEFDL 457
G KE+ LP E DE++ T + + +LE DL
Sbjct: 419 GLKELENGTLPVTESVDEEVSTDNEVEYDSDDERDRLEADL 459
>SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 412
Score = 27.1 bits (57), Expect = 3.2
Identities = 18/69 (26%), Positives = 34/69 (49%), Gaps = 7/69 (10%)
Frame = -3
Query: 690 LVEVGLEKQNKVAPIHNIL--VEEVHMLVVVRLATVQS-----VGYKEVAKHQLPYDEKQ 532
L E+ +E ++AP NI+ + + L L T + + Y ++ + PYD ++
Sbjct: 178 LRELDIELMRRLAPRVNIIPAIAKADSLTAQELQTTKEMINADIEYYKIPVYDFPYDIEE 237
Query: 531 DEQLLTWLS 505
DE+ + LS
Sbjct: 238 DEEAIINLS 246
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 26.2 bits (55), Expect = 5.5
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
Frame = +2
Query: 110 LNVIYNQHNNCIMSLNTAHADHGVLIHAGECI-ILFSDNVS 229
L+ + NQ + S+N ADH L+ A EC IL +D+ S
Sbjct: 1396 LHELVNQFLTGVASINHPIADHVFLLCAQECCRILLTDSKS 1436
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.2 bits (55), Expect = 5.5
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = +3
Query: 654 PPYSAFPDQPPPNSVFVSNTPPPYPGVTGASYP 752
P + P PP + PPP PGV GA P
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPP 776
>SPBC30B4.04c |sol1||SWI/SNF complex subunit
Sol1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 865
Score = 25.4 bits (53), Expect = 9.7
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = +3
Query: 654 PPYSAFPDQPPPNSVFVSNTPPPYPGVTGASY 749
PPY + P +P P +V S++ P +Y
Sbjct: 356 PPYPSAPTRPTPPTVQTSSSAAPVDSAEPVAY 387
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 25.4 bits (53), Expect = 9.7
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -3
Query: 795 GTHKPHHPRKTQGRSDKKLQSRQGREVGYSTQIL 694
GTHKP+H + + K QS + ++ S+QIL
Sbjct: 264 GTHKPNHSENAELKDIK--QSSELSDLSLSSQIL 295
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,197,673
Number of Sequences: 5004
Number of extensions: 66414
Number of successful extensions: 196
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 396433620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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