BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021034 (844 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 130 3e-31 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 69 1e-12 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 57 3e-09 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 55 1e-08 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 55 1e-08 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 48 2e-06 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 47 3e-06 SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 38 0.002 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 33 0.051 SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 27 3.3 SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 4.4 SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizos... 26 7.7 SPBC1703.03c |||armadillo repeat protein, unknown biological rol... 26 7.7 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 130 bits (313), Expect = 3e-31 Identities = 56/84 (66%), Positives = 76/84 (90%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDRLGA 182 PGH+DFT+EVERALRVLDGA+LVLC+V GVQSQT+TV+RQM+RYNVP ++F+NK+DR+GA Sbjct: 153 PGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDRMGA 212 Query: 183 NPERVLKQMRSKLKHNAAFIHLPI 254 +P +V++Q+ +KLK AA + +PI Sbjct: 213 DPWKVIQQINTKLKIPAAAVQIPI 236 Score = 109 bits (263), Expect = 4e-25 Identities = 60/111 (54%), Positives = 75/111 (67%) Frame = +1 Query: 511 FTPVLLGTALKNKGIQPLLDAVLTYLPHPGEVENTALLNESKDGEGQTIVLDPTRDNKKP 690 FTPVL+G+AL N G+Q +LDAV YLP+P EVEN AL + D E + + L P+ ++KP Sbjct: 324 FTPVLMGSALSNVGVQSVLDAVCDYLPNPSEVENIAL--NAADSE-KPVSLVPS--SEKP 378 Query: 691 FVGLAFKLEVSQFGQLTYLRCYPGC*REVTIYSTPGLTKKVKISRLVRMHS 843 V LAFKLE +FGQLTYLR Y G + TKK+K+SRLVRMHS Sbjct: 379 LVALAFKLEEGRFGQLTYLRIYQGTLKRGNYIYNVNSTKKIKVSRLVRMHS 429 Score = 65.7 bits (153), Expect = 8e-12 Identities = 30/84 (35%), Positives = 55/84 (65%), Gaps = 1/84 (1%) Frame = +2 Query: 260 EKECQGILDLVEEKAIYFDGEYGEHVRLDE-IPQDRRTEVKERRHELIEHLSNVDETLGE 436 E + +G++DL++ +AIY G GE + + + +P++ KE+R LIE L+++DE + + Sbjct: 239 EDKLEGVVDLIQMRAIYNRGSKGEKIEISQQVPENLIELAKEKRSALIEKLADLDEEIAD 298 Query: 437 LFLEEKTPTVNDLKQALRRSTLKR 508 +++ E+ PT L A+RR+TL R Sbjct: 299 IYVMEEDPTPEQLMGAIRRTTLAR 322 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 68.5 bits (160), Expect = 1e-12 Identities = 29/73 (39%), Positives = 49/73 (67%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDRLGA 182 PGH DFT EVER++ VLDGA+ ++ GV++QT V +Q + +P + F+NK+DR+G+ Sbjct: 101 PGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQATKRGIPKVIFVNKMDRVGS 160 Query: 183 NPERVLKQMRSKL 221 + ++ + + L Sbjct: 161 SLGSTIRSIYTNL 173 Score = 49.6 bits (113), Expect = 5e-07 Identities = 37/112 (33%), Positives = 53/112 (47%), Gaps = 2/112 (1%) Frame = +1 Query: 499 IKTTFTPVLLGTALKNKGIQPLLDAVLTYLPHPGEVENTALLNESKDGEGQTIV-LDPTR 675 I PVL G++LKN +QP++DA++ YLP P E N SK+ I+ LD Sbjct: 281 ISGNVVPVLCGSSLKNIAVQPIMDAIIDYLPSPVEFYEK---NASKETSSDKIISLD--- 334 Query: 676 DNKKPFVGLAFK-LEVSQFGQLTYLRCYPGC*REVTIYSTPGLTKKVKISRL 828 K+P + FK + + G LTY+R G + P K + RL Sbjct: 335 --KRPLLAKIFKVIHHASRGILTYVRVNEGTLSRGMMMFNPRTKKSERAIRL 384 Score = 41.5 bits (93), Expect = 1e-04 Identities = 25/71 (35%), Positives = 40/71 (56%), Gaps = 6/71 (8%) Frame = +2 Query: 260 EKECQGILDLVEEKAIYFD------GEYGEHVRLDEIPQDRRTEVKERRHELIEHLSNVD 421 E++ GILD++++K I +D G G HV+ IP+ E R+ L+ L +VD Sbjct: 192 ERKFLGILDILQQKMILWDTSDNKLGTDGTHVQELPIPESHMERFIEARNALVMSLCDVD 251 Query: 422 ETLGELFLEEK 454 ETL + +LE + Sbjct: 252 ETLCDEYLENE 262 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 57.2 bits (132), Expect = 3e-09 Identities = 29/58 (50%), Positives = 39/58 (67%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDRL 176 PGHVDF+ EV A R+ DGA +++ +V GV SQT+TV RQ + + INK+DRL Sbjct: 102 PGHVDFSSEVSSASRLCDGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDRL 159 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 55.2 bits (127), Expect = 1e-08 Identities = 28/57 (49%), Positives = 38/57 (66%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDR 173 PGHVDF+ EV ALRV DGA++V+ ++ GV QT TV RQ + + +NK+DR Sbjct: 106 PGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDR 162 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 55.2 bits (127), Expect = 1e-08 Identities = 28/57 (49%), Positives = 38/57 (66%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDR 173 PGHVDF+ EV ALRV DGA++V+ ++ GV QT TV RQ + + +NK+DR Sbjct: 106 PGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDR 162 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 48.0 bits (109), Expect = 2e-06 Identities = 25/70 (35%), Positives = 41/70 (58%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDRLGA 182 PGHVDF EV +L +G IL++ + G+Q+QTL+ N+ + +NK+D A Sbjct: 132 PGHVDFRAEVMHSLAACEGCILLVDASQGIQAQTLSNFYMAFSQNLVIIPVLNKVDLPTA 191 Query: 183 NPERVLKQMR 212 + +R L Q++ Sbjct: 192 DVDRTLIQVQ 201 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 47.2 bits (107), Expect = 3e-06 Identities = 21/58 (36%), Positives = 34/58 (58%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDRL 176 PGHVDF EV + + DG +LV+ + GV T + + +++P + +NK+DRL Sbjct: 217 PGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKVDRL 274 >SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1079 Score = 37.9 bits (84), Expect = 0.002 Identities = 20/58 (34%), Positives = 32/58 (55%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQTLTVNRQMKRYNVPCLAFINKLDRL 176 PGH FT R + + AILV+ + G++ QT+ R ++ P + +NK+DRL Sbjct: 557 PGHESFTNLRSRGTSLCNIAILVIDIMHGLEPQTIESIRLLRDQKTPFVVALNKVDRL 614 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 33.1 bits (72), Expect = 0.051 Identities = 24/77 (31%), Positives = 39/77 (50%), Gaps = 4/77 (5%) Frame = +3 Query: 3 PGHVDFTVEVERALRVLDGAILVLCSVGGVQSQT---LTVNRQMKRYNVPCLAFINKLDR 173 PGH D+ + +DGAI+V+ + G QT L + RQ+ + + +INK+D Sbjct: 124 PGHADYIKNMITGAATMDGAIIVVSATDGQMPQTREHLLLARQVGVKQI--VVYINKVDM 181 Query: 174 LGANP-ERVLKQMRSKL 221 + + E V +MR L Sbjct: 182 VEPDMIELVEMEMRELL 198 >SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 27.1 bits (57), Expect = 3.3 Identities = 13/46 (28%), Positives = 25/46 (54%) Frame = +3 Query: 87 GVQSQTLTVNRQMKRYNVPCLAFINKLDRLGANPERVLKQMRSKLK 224 G + V+ Q K+YN+ F+ KL + G N + + ++M+ L+ Sbjct: 212 GKAKEQKIVSEQSKKYNMFEEGFLAKLHKPGGNTQLIPERMKEHLQ 257 >SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces pombe|chr 1|||Manual Length = 949 Score = 26.6 bits (56), Expect = 4.4 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = -1 Query: 88 PPTEHNTNIAPSKTLNALSTSTVKS 14 PPTE + ++APS + +A ST + KS Sbjct: 15 PPTESSAHLAPSFSDSATSTLSFKS 39 >SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 197 Score = 25.8 bits (54), Expect = 7.7 Identities = 20/59 (33%), Positives = 25/59 (42%) Frame = -3 Query: 677 SLVGSNTMVWPSPSLLSFNKAVFSTSPGCGRYVSTASSKG*IPLFFRAVPRSTGVNVVL 501 SLV S T+ SPS +S ++ S S VST+S F V ST L Sbjct: 129 SLVSSTTLTSSSPSAVSSTTSIPSISSTISSSVSTSSFTSLSSSGFSTVLSSTNTTSAL 187 >SPBC1703.03c |||armadillo repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 664 Score = 25.8 bits (54), Expect = 7.7 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = -1 Query: 214 ERICFSTLSGLAPNLSSLLIN 152 +R FS L +A N+SSLL+N Sbjct: 151 DRSTFSCLCAIAENISSLLVN 171 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,653,931 Number of Sequences: 5004 Number of extensions: 79277 Number of successful extensions: 245 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 231 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 242 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 416455520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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