Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021031
(738 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 139 3e-34
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 136 3e-33
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 126 4e-30
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 124 2e-29
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 116 5e-27
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 60 4e-10
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 44 2e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.30
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.91
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 28 1.6
SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12 |Schizos... 26 4.9
SPBC19C2.12 |mrpl51||mitochondrial ribosomal protein subunit L51... 25 8.5
SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.5
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 8.5
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 8.5
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 139 bits (337), Expect = 3e-34
Identities = 66/76 (86%), Positives = 71/76 (93%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 688
DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGTFDVS+LT
Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSLLT 209
Query: 689 IEDGIFEVKSTAGDTH 736
IE+GIFEVK+TAGDTH
Sbjct: 210 IEEGIFEVKATAGDTH 225
Score = 135 bits (327), Expect = 5e-33
Identities = 62/88 (70%), Positives = 72/88 (81%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKXEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 422
P IFDAKRLIGRK +D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPAYF 506
VL KM+ETAEAYLG V +AV+TVPAYF
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYF 148
Score = 117 bits (281), Expect = 2e-27
Identities = 53/61 (86%), Positives = 58/61 (95%)
Frame = +1
Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 253 T 255
T
Sbjct: 64 T 64
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 136 bits (329), Expect = 3e-33
Identities = 65/76 (85%), Positives = 70/76 (92%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 688
DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGTFDVS+LT
Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSLLT 209
Query: 689 IEDGIFEVKSTAGDTH 736
IE+GIFEVK+TAGDTH
Sbjct: 210 IEEGIFEVKATAGDTH 225
Score = 128 bits (308), Expect = 1e-30
Identities = 58/88 (65%), Positives = 70/88 (79%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKXEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 422
P IFDAKRLIGR+ D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPAYF 506
VL KM+E+AEA+LG V +AV+TVPAYF
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYF 148
Score = 117 bits (281), Expect = 2e-27
Identities = 53/61 (86%), Positives = 58/61 (95%)
Frame = +1
Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 253 T 255
T
Sbjct: 64 T 64
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 126 bits (303), Expect = 4e-30
Identities = 60/76 (78%), Positives = 66/76 (86%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 688
DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGTFDVS+L
Sbjct: 154 DSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDVSLLK 213
Query: 689 IEDGIFEVKSTAGDTH 736
I+ G+FEV +TAGDTH
Sbjct: 214 IQGGVFEVLATAGDTH 229
Score = 115 bits (276), Expect = 8e-27
Identities = 53/88 (60%), Positives = 65/88 (73%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKXEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 422
P+ +FDAKRLIGR+ ED Q D+KHWPF+V+ G P I+V Y GE K F +E+S+M
Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPAYF 506
VLTKMKE +EA L K V+ AVITVPAYF
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYF 152
Score = 104 bits (250), Expect = 1e-23
Identities = 47/61 (77%), Positives = 53/61 (86%)
Frame = +1
Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67
Query: 253 T 255
T
Sbjct: 68 T 68
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 124 bits (298), Expect = 2e-29
Identities = 55/76 (72%), Positives = 72/76 (94%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 688
D+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T E++++++DLGGGTFDVS+L+
Sbjct: 183 DAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGTFDVSLLS 241
Query: 689 IEDGIFEVKSTAGDTH 736
I++G+FEV +T+GDTH
Sbjct: 242 IDNGVFEVLATSGDTH 257
Score = 108 bits (259), Expect = 9e-25
Identities = 49/97 (50%), Positives = 70/97 (72%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKXEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 422
P+ IFD KRLIGRK ++ T+ D+K +PF +V+ +P ++V G+ K F PEE+S+M
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPAYFKTLKDKPQK 533
+L+KMK+TAEAYLGK V +AV+TVPAYF + + K
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190
Score = 102 bits (245), Expect = 5e-23
Identities = 45/60 (75%), Positives = 52/60 (86%)
Frame = +1
Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 116 bits (278), Expect = 5e-27
Identities = 54/76 (71%), Positives = 65/76 (85%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 688
DSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGTFD+SIL
Sbjct: 196 DSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGTFDISILE 253
Query: 689 IEDGIFEVKSTAGDTH 736
+ +G+FEV+ST GDTH
Sbjct: 254 LNNGVFEVRSTNGDTH 269
Score = 79.4 bits (187), Expect = 5e-16
Identities = 37/88 (42%), Positives = 54/88 (61%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKXEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSM 422
P+ F KRLIGR+ ++ VQ D+K P+++V + KT+ P ++
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPAYF 506
+L+KM+ETA YLGK V+NAV+TVPAYF
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYF 194
Score = 78.6 bits (185), Expect = 9e-16
Identities = 36/66 (54%), Positives = 47/66 (71%), Gaps = 1/66 (1%)
Frame = +1
Query: 64 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 240
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 241 NPNNTY 258
NP NT+
Sbjct: 109 NPENTF 114
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 59.7 bits (138), Expect = 4e-10
Identities = 33/78 (42%), Positives = 44/78 (56%), Gaps = 4/78 (5%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGGTFDV 676
D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G + V
Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNYSV 213
Query: 677 SILTIEDGIFEVKSTAGD 730
SI+ G F +KST D
Sbjct: 214 SIVEFSRGQFHIKSTVCD 231
Score = 54.0 bits (124), Expect = 2e-08
Identities = 23/65 (35%), Positives = 41/65 (63%)
Frame = +1
Query: 61 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 240
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62
Query: 241 NPNNT 255
N NT
Sbjct: 63 NFRNT 67
Score = 40.7 bits (91), Expect = 2e-04
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
Frame = +3
Query: 267 KRLIGRKXEDATVQ-ADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 443
KRL GR +D ++ + ++ G KV Y E+ F ++ + TK+K
Sbjct: 72 KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131
Query: 444 TAEAYLGKTVQNAVITVPAYFKTLK 518
AEA L +V + VI++PA+F ++
Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQ 156
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 44.4 bits (100), Expect = 2e-05
Identities = 18/60 (30%), Positives = 34/60 (56%)
Frame = +1
Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N +N+
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86
Score = 33.1 bits (72), Expect = 0.042
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +3
Query: 384 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFKTLKDK 524
++K E S L ++ E+AE +LG V V++VP YF + K
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 30.3 bits (65), Expect = 0.30
Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +1
Query: 76 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82
Query: 253 TYSMPNV 273
+S+ NV
Sbjct: 83 AHSIRNV 89
Score = 25.8 bits (54), Expect = 6.4
Identities = 11/45 (24%), Positives = 24/45 (53%)
Frame = +3
Query: 384 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFKTLK 518
+++ + EE+ +M + AE + + + V+TVP +F L+
Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQ 175
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.91
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 68 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 163
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +1
Query: 172 PSYVAFTDTERLIGDAAKNQVAMNPNNTYSMPNVSSDVSXKMLLC 306
P +A T ER++ AAK A+N Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 26.2 bits (55), Expect = 4.9
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +1
Query: 385 KTKPFSPRKSVPWCLRK*RKLPKLISAKLCRMQSSRFP 498
+ K KSVP C + + K IS K+ R+QS+ P
Sbjct: 16 RPKDIKSIKSVPICEKHRASVVKDISRKISRIQSATLP 53
>SPBC19C2.12 |mrpl51||mitochondrial ribosomal protein subunit
L51|Schizosaccharomyces pombe|chr 2|||Manual
Length = 145
Score = 25.4 bits (53), Expect = 8.5
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -3
Query: 292 SXLRPMRRLASNMCCWGSSPPG 227
S RP RR+ + C WG S G
Sbjct: 18 SFTRPCRRIEFSYCNWGGSSKG 39
>SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 600
Score = 25.4 bits (53), Expect = 8.5
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 157 GNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTYS 261
GNR+T ++ L DAAKN +A + TYS
Sbjct: 349 GNRSTTNFSIINSGLYLKKDAAKNLLAKQFDATYS 383
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 25.4 bits (53), Expect = 8.5
Identities = 17/66 (25%), Positives = 31/66 (46%)
Frame = +3
Query: 54 QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 233
+NGK T +R +S + W P++E R G D + V R + + +EP
Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531
Query: 234 GDEPQQ 251
++P++
Sbjct: 532 -EKPEE 536
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.4 bits (53), Expect = 8.5
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -3
Query: 154 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 50
G W SP S + H ++++ +F +RVL C+
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,171,992
Number of Sequences: 5004
Number of extensions: 66406
Number of successful extensions: 264
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 259
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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