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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021025
         (730 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe...    97   2e-21
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    29   0.51 
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi...    27   2.1  
SPAPB24D3.06c |||DUF1749 family protein|Schizosaccharomyces pomb...    27   2.7  
SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF...    27   3.6  
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce...    26   6.3  
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    25   8.4  
SPCC338.14 |||adenosine kinase |Schizosaccharomyces pombe|chr 3|...    25   8.4  

>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 526

 Score = 97.1 bits (231), Expect = 2e-21
 Identities = 44/72 (61%), Positives = 58/72 (80%)
 Frame = +3

Query: 510 LVGGSVPVFDEFVLCLGLLNKIISLDEISGVLICEAGCILESLDTYVRERNLIMPLDLGA 689
           LVGGSVPVFDE VL LGL+N+I + DEISGV+  ++G ILE+ D ++ E+  + PLDLGA
Sbjct: 132 LVGGSVPVFDEIVLNLGLMNQIHTFDEISGVITLDSGVILENADNFLAEKGYMFPLDLGA 191

Query: 690 KGTCQIGGNAST 725
           KG+CQ+GG A+T
Sbjct: 192 KGSCQVGGCAAT 203



 Score = 82.6 bits (195), Expect = 5e-17
 Identities = 38/84 (45%), Positives = 57/84 (67%), Gaps = 7/84 (8%)
 Frame = +1

Query: 277 QFGTVQESDINYFKSILSEERVL-------TDENDVLPFNIDWIKNCRGQSKVVLKPRST 435
           ++  + E D+  FKSI+ ++  L       TD  D+  FNIDW+   RG++++ LKP++T
Sbjct: 47  KYAKLSEQDVQVFKSIIGKDGSLIDGLDKSTDPADLDAFNIDWMNKYRGKTQLALKPKTT 106

Query: 436 EEVSKIIKYCNDKHLAVCPQGGNT 507
           ++VS+I+KYCN K LAV PQGGNT
Sbjct: 107 QQVSEILKYCNQKKLAVVPQGGNT 130


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 29.5 bits (63), Expect = 0.51
 Identities = 19/62 (30%), Positives = 33/62 (53%), Gaps = 3/62 (4%)
 Frame = +1

Query: 286  TVQESDINYFKSILSEERVLTDENDV---LPFNIDWIKNCRGQSKVVLKPRSTEEVSKII 456
            T+QE D     S  + E  L D++ +   L  NI+ + N   + K  +K  STE+ S+I+
Sbjct: 1517 TIQELDHEITASKNNYEGKLNDKDSIIRDLSENIEQLNNLLAEEKSAVKRLSTEKESEIL 1576

Query: 457  KY 462
            ++
Sbjct: 1577 QF 1578


>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 865

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = -3

Query: 710 TNLTSTFGP*VQWHY*IALTDICI*TFKDASRFTNENT 597
           T +T+ F P  +WH+     D  +  FK A  F +E+T
Sbjct: 433 TEVTNAFAPNKRWHF-----DTLLRVFKSAGNFVSEST 465


>SPAPB24D3.06c |||DUF1749 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 316

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 16/43 (37%), Positives = 22/43 (51%)
 Frame = -1

Query: 517 PTSQCSRLADKPPNVCHYNT**FSKPPLCFWVLKQLLTALGNF 389
           PT+  S L+   P   H N    S P    W++ QL+TAL +F
Sbjct: 272 PTTSNSSLSGIIPGATH-NVGPKSSPEALKWLINQLITALRSF 313


>SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease
           XPF|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 892

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 12/47 (25%), Positives = 26/47 (55%)
 Frame = +3

Query: 495 RREH*LVGGSVPVFDEFVLCLGLLNKIISLDEISGVLICEAGCILES 635
           +RE   + G +      +L + LL KII  ++I+G+++  A  ++ +
Sbjct: 89  KREKSYLEGGIFAITSRILVMDLLTKIIPTEKITGIVLLHADRVVST 135


>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 747

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 13/46 (28%), Positives = 24/46 (52%)
 Frame = +3

Query: 552 CLGLLNKIISLDEISGVLICEAGCILESLDTYVRERNLIMPLDLGA 689
           CL +LN   +  +IS + I    C + S D ++     I+ L++G+
Sbjct: 643 CLSMLNNENTDIKISSLNIARLLCQMTSSDAFIESAKNILILEMGS 688


>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 18/69 (26%), Positives = 30/69 (43%)
 Frame = -1

Query: 643 VSKLSRMHPASQMRTPDISSKLIILFRRPRQRTNSSKTGTEPPTSQCSRLADKPPNVCHY 464
           V+  S    +++  T  ++S  I     P   +++S T T PPT+  +     PP     
Sbjct: 44  VTTTSFSSASTETSTHSVTSTNITSIVPPPSTSHNSTTTTVPPTTSMNTTTTVPPTT-SL 102

Query: 463 NT**FSKPP 437
           NT   + PP
Sbjct: 103 NTTTTTAPP 111


>SPCC338.14 |||adenosine kinase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 340

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
 Frame = +2

Query: 449 KLSSIVMTNIWRFVRKAGTL-TGG-RLSAGLR*IRSLSWSSEQNNK 580
           KL  +   N+W+FV +A  +  GG  L+     +  L+  + +NNK
Sbjct: 138 KLKDLQQPNVWKFVEEAKVIYVGGFHLTVSPESMLCLAQHANENNK 183


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,003,902
Number of Sequences: 5004
Number of extensions: 63356
Number of successful extensions: 190
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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