BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021022
(810 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10220.1 68416.m01223 tubulin folding cofactor B identical to... 64 1e-10
At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferlin... 43 3e-04
At5g52280.1 68418.m06488 protein transport protein-related low s... 33 0.22
At4g24700.1 68417.m03535 expressed protein 32 0.52
At3g22080.1 68416.m02786 meprin and TRAF homology domain-contain... 31 1.2
At3g52640.2 68416.m05800 nicastrin-related contains weak similar... 29 3.7
At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 29 3.7
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 28 6.4
At2g23270.1 68415.m02779 expressed protein ; similar to GP|24648... 28 8.4
>At3g10220.1 68416.m01223 tubulin folding cofactor B identical to
tubulin folding cofactor B GI:20514259 from [Arabidopsis
thaliana]; identical to cDNA tubulin folding cofactor B
GI:20514258
Length = 243
Score = 63.7 bits (148), Expect = 1e-10
Identities = 33/84 (39%), Positives = 47/84 (55%), Gaps = 3/84 (3%)
Frame = +3
Query: 462 VSANWTTKVFRPQRDITEDTDSFI-IGERVWVG-GTKPGQIAYIGETQ-FAPGEWAGIVL 632
VS N + + + ED + I +G+R V G K G + Y+G + PG W GI
Sbjct: 136 VSQNPVAAEAKTKENYMEDLCANIKVGDRCQVEPGEKRGMVKYVGRAESLGPGYWVGIQY 195
Query: 633 DEPIGKNDGSVAGVRYFQCPEKRG 704
DEP+GK+DG V G R+F+CP +G
Sbjct: 196 DEPLGKHDGMVKGTRFFECPRLQG 219
>At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferling
(PFI) almost identical to tubulin folding cofactor E
(Pfifferling; PFI) GI:20514267 from [Arabidopsis
thaliana]; identical to cDNA tubulin folding cofactor E,
GI:20514266
Length = 531
Score = 42.7 bits (96), Expect = 3e-04
Identities = 23/57 (40%), Positives = 34/57 (59%), Gaps = 3/57 (5%)
Frame = +3
Query: 522 DSFIIGERVWV--GGTKPGQIAYIGETQFAPGEWAGIVLDEP-IGKNDGSVAGVRYF 683
+SFIIG+RV + G + Y+G+ + G W G+ D+ GK++GSV GV YF
Sbjct: 8 ESFIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYF 64
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 33.1 bits (72), Expect = 0.22
Identities = 21/84 (25%), Positives = 45/84 (53%), Gaps = 4/84 (4%)
Frame = +2
Query: 254 REMIDKIQANVEEVKKKHSAILSA---PQSDENGSSTDTLRKLSDDLSR-KHLSASSTFS 421
R+ + ++ ++ +++ + IL A +S ENG + L KLSD+L+ K+ ++S
Sbjct: 756 RKQVSNLKVDIRRKEEEMTKILDARMEARSQENGHKEENLSKLSDELAYCKNKNSSMERE 815
Query: 422 ATSMDALWEKHARRLSELDYEGLQ 493
M+ + + + R +E++ E Q
Sbjct: 816 LKEMEERYSEISLRFAEVEGERQQ 839
>At4g24700.1 68417.m03535 expressed protein
Length = 143
Score = 31.9 bits (69), Expect = 0.52
Identities = 25/79 (31%), Positives = 35/79 (44%)
Frame = +2
Query: 248 RNREMIDKIQANVEEVKKKHSAILSAPQSDENGSSTDTLRKLSDDLSRKHLSASSTFSAT 427
+ RE D+ + EE KK+ + +A Q+ + S T L D RKH S
Sbjct: 3 KKREAKDENEEEEEEKKKRLELMKAAAQAWLSHSQTSKHTVLEFDAQRKHAFVKGKASRF 62
Query: 428 SMDALWEKHARRLSELDYE 484
+AL KH S LD+E
Sbjct: 63 KTEALSAKH--HPSFLDWE 79
>At3g22080.1 68416.m02786 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 595
Score = 30.7 bits (66), Expect = 1.2
Identities = 12/49 (24%), Positives = 27/49 (55%), Gaps = 2/49 (4%)
Frame = +3
Query: 411 VRSALLRWMLFGRNTRVVSA--NWTTKVFRPQRDITEDTDSFIIGERVW 551
V +L +W + N +++ +W+ K F+ ++ ++D F++G R W
Sbjct: 116 VAPSLTKWEVVSFNQKILDPKFSWSLKKFKELKEELYNSDKFLVGGRQW 164
>At3g52640.2 68416.m05800 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 705
Score = 29.1 bits (62), Expect = 3.7
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +3
Query: 582 YIGETQFAPGEWAGIVLDEPIGK 650
YI T PG +AG++L EP K
Sbjct: 535 YISPTNTCPGNYAGVILGEPSSK 557
>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 676
Score = 29.1 bits (62), Expect = 3.7
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +3
Query: 582 YIGETQFAPGEWAGIVLDEPIGK 650
YI T PG +AG++L EP K
Sbjct: 506 YISPTNTCPGNYAGVILGEPSSK 528
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 28.3 bits (60), Expect = 6.4
Identities = 18/65 (27%), Positives = 31/65 (47%)
Frame = +2
Query: 308 SAILSAPQSDENGSSTDTLRKLSDDLSRKHLSASSTFSATSMDALWEKHARRLSELDYEG 487
S L + + SS+ T + S R SASS+ S + +A+ +++ EL +G
Sbjct: 40 SPALRCASAASSSSSSATTAETSKPSGRNRRSASSSNSTSDREAIRSIRLKKVEELRGQG 99
Query: 488 LQTTA 502
L+ A
Sbjct: 100 LEPYA 104
>At2g23270.1 68415.m02779 expressed protein ; similar to
GP|2464853|gnl|PID|e353149|Z99707
Length = 86
Score = 27.9 bits (59), Expect = 8.4
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +1
Query: 379 RPI*KTPISQQYVQRYFDGCSLGETRASSQRTG 477
RP+ T + V ++FDG SLG + S +G
Sbjct: 26 RPLGLTKAEENLVAKFFDGLSLGAIKESGPSSG 58
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,115,523
Number of Sequences: 28952
Number of extensions: 327778
Number of successful extensions: 1082
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1034
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1078
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1843581600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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