BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021022 (810 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g10220.1 68416.m01223 tubulin folding cofactor B identical to... 64 1e-10 At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferlin... 43 3e-04 At5g52280.1 68418.m06488 protein transport protein-related low s... 33 0.22 At4g24700.1 68417.m03535 expressed protein 32 0.52 At3g22080.1 68416.m02786 meprin and TRAF homology domain-contain... 31 1.2 At3g52640.2 68416.m05800 nicastrin-related contains weak similar... 29 3.7 At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 29 3.7 At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 28 6.4 At2g23270.1 68415.m02779 expressed protein ; similar to GP|24648... 28 8.4 >At3g10220.1 68416.m01223 tubulin folding cofactor B identical to tubulin folding cofactor B GI:20514259 from [Arabidopsis thaliana]; identical to cDNA tubulin folding cofactor B GI:20514258 Length = 243 Score = 63.7 bits (148), Expect = 1e-10 Identities = 33/84 (39%), Positives = 47/84 (55%), Gaps = 3/84 (3%) Frame = +3 Query: 462 VSANWTTKVFRPQRDITEDTDSFI-IGERVWVG-GTKPGQIAYIGETQ-FAPGEWAGIVL 632 VS N + + + ED + I +G+R V G K G + Y+G + PG W GI Sbjct: 136 VSQNPVAAEAKTKENYMEDLCANIKVGDRCQVEPGEKRGMVKYVGRAESLGPGYWVGIQY 195 Query: 633 DEPIGKNDGSVAGVRYFQCPEKRG 704 DEP+GK+DG V G R+F+CP +G Sbjct: 196 DEPLGKHDGMVKGTRFFECPRLQG 219 >At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferling (PFI) almost identical to tubulin folding cofactor E (Pfifferling; PFI) GI:20514267 from [Arabidopsis thaliana]; identical to cDNA tubulin folding cofactor E, GI:20514266 Length = 531 Score = 42.7 bits (96), Expect = 3e-04 Identities = 23/57 (40%), Positives = 34/57 (59%), Gaps = 3/57 (5%) Frame = +3 Query: 522 DSFIIGERVWV--GGTKPGQIAYIGETQFAPGEWAGIVLDEP-IGKNDGSVAGVRYF 683 +SFIIG+RV + G + Y+G+ + G W G+ D+ GK++GSV GV YF Sbjct: 8 ESFIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYF 64 >At5g52280.1 68418.m06488 protein transport protein-related low similarity to SP|P25386 Intracellular protein transport protein USO1 {Saccharomyces cerevisiae} Length = 853 Score = 33.1 bits (72), Expect = 0.22 Identities = 21/84 (25%), Positives = 45/84 (53%), Gaps = 4/84 (4%) Frame = +2 Query: 254 REMIDKIQANVEEVKKKHSAILSA---PQSDENGSSTDTLRKLSDDLSR-KHLSASSTFS 421 R+ + ++ ++ +++ + IL A +S ENG + L KLSD+L+ K+ ++S Sbjct: 756 RKQVSNLKVDIRRKEEEMTKILDARMEARSQENGHKEENLSKLSDELAYCKNKNSSMERE 815 Query: 422 ATSMDALWEKHARRLSELDYEGLQ 493 M+ + + + R +E++ E Q Sbjct: 816 LKEMEERYSEISLRFAEVEGERQQ 839 >At4g24700.1 68417.m03535 expressed protein Length = 143 Score = 31.9 bits (69), Expect = 0.52 Identities = 25/79 (31%), Positives = 35/79 (44%) Frame = +2 Query: 248 RNREMIDKIQANVEEVKKKHSAILSAPQSDENGSSTDTLRKLSDDLSRKHLSASSTFSAT 427 + RE D+ + EE KK+ + +A Q+ + S T L D RKH S Sbjct: 3 KKREAKDENEEEEEEKKKRLELMKAAAQAWLSHSQTSKHTVLEFDAQRKHAFVKGKASRF 62 Query: 428 SMDALWEKHARRLSELDYE 484 +AL KH S LD+E Sbjct: 63 KTEALSAKH--HPSFLDWE 79 >At3g22080.1 68416.m02786 meprin and TRAF homology domain-containing protein / MATH domain-containing protein weak similarity to ubiquitin-specific protease 12 [Arabidopsis thaliana] GI:11993471; contains Pfam profile PF00917: MATH domain Length = 595 Score = 30.7 bits (66), Expect = 1.2 Identities = 12/49 (24%), Positives = 27/49 (55%), Gaps = 2/49 (4%) Frame = +3 Query: 411 VRSALLRWMLFGRNTRVVSA--NWTTKVFRPQRDITEDTDSFIIGERVW 551 V +L +W + N +++ +W+ K F+ ++ ++D F++G R W Sbjct: 116 VAPSLTKWEVVSFNQKILDPKFSWSLKKFKELKEELYNSDKFLVGGRQW 164 >At3g52640.2 68416.m05800 nicastrin-related contains weak similarity to Nicastrin precursor (Swiss-Prot:Q92542) [Homo sapiens] Length = 705 Score = 29.1 bits (62), Expect = 3.7 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = +3 Query: 582 YIGETQFAPGEWAGIVLDEPIGK 650 YI T PG +AG++L EP K Sbjct: 535 YISPTNTCPGNYAGVILGEPSSK 557 >At3g52640.1 68416.m05799 nicastrin-related contains weak similarity to Nicastrin precursor (Swiss-Prot:Q92542) [Homo sapiens] Length = 676 Score = 29.1 bits (62), Expect = 3.7 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = +3 Query: 582 YIGETQFAPGEWAGIVLDEPIGK 650 YI T PG +AG++L EP K Sbjct: 506 YISPTNTCPGNYAGVILGEPSSK 528 >At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) family protein similar to SP|Q9RHV9 Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus stearothermophilus}; contains Pfam profile: PF00152 tRNA synthetases class II (D, K and N) Length = 602 Score = 28.3 bits (60), Expect = 6.4 Identities = 18/65 (27%), Positives = 31/65 (47%) Frame = +2 Query: 308 SAILSAPQSDENGSSTDTLRKLSDDLSRKHLSASSTFSATSMDALWEKHARRLSELDYEG 487 S L + + SS+ T + S R SASS+ S + +A+ +++ EL +G Sbjct: 40 SPALRCASAASSSSSSATTAETSKPSGRNRRSASSSNSTSDREAIRSIRLKKVEELRGQG 99 Query: 488 LQTTA 502 L+ A Sbjct: 100 LEPYA 104 >At2g23270.1 68415.m02779 expressed protein ; similar to GP|2464853|gnl|PID|e353149|Z99707 Length = 86 Score = 27.9 bits (59), Expect = 8.4 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +1 Query: 379 RPI*KTPISQQYVQRYFDGCSLGETRASSQRTG 477 RP+ T + V ++FDG SLG + S +G Sbjct: 26 RPLGLTKAEENLVAKFFDGLSLGAIKESGPSSG 58 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,115,523 Number of Sequences: 28952 Number of extensions: 327778 Number of successful extensions: 1082 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 1034 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1078 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1843581600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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