BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021018 (815 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g42890.1 68418.m05227 sterol carrier protein 2 (SCP-2) family... 36 0.024 At3g10010.1 68416.m01201 HhH-GPD base excision DNA repair family... 29 3.7 At3g47290.1 68416.m05139 phosphoinositide-specific phospholipase... 28 8.5 >At5g42890.1 68418.m05227 sterol carrier protein 2 (SCP-2) family protein similar to sterol carrier protein-2 [Homo sapiens] GI:432973; contains Pfam profile PF02036: SCP-2 sterol transfer family Length = 123 Score = 36.3 bits (80), Expect = 0.024 Identities = 14/28 (50%), Positives = 21/28 (75%) Frame = +1 Query: 520 SGKLNPQKAFFQGKIKIQGNMGLAMKLT 603 +GK+NPQ AF +G +KI+G++ A K T Sbjct: 86 TGKMNPQMAFIRGAMKIKGSLSAAQKFT 113 Score = 28.7 bits (61), Expect = 4.9 Identities = 20/70 (28%), Positives = 36/70 (51%), Gaps = 3/70 (4%) Frame = +2 Query: 305 MKILEEAMQTDQDNLI-EKVRGIYGFKVRNGPDGAEGY-WVINAKEGK-GKVSYNGSEKP 475 M +++E + TD + EK+ +Y + G E ++++ K+G+ K Y G K Sbjct: 12 MDMMKEHLSTDAGKEVTEKIGLVYQINIAPKKLGFEEVTYIVDLKKGEVTKGKYEGG-KV 70 Query: 476 DVTFTISDED 505 D TF+ D+D Sbjct: 71 DATFSFKDDD 80 >At3g10010.1 68416.m01201 HhH-GPD base excision DNA repair family protein similar to DEMETER protein [Arabidopsis thaliana] GI:21743571; contains Pfam profile PF00730: HhH-GPD superfamily base excision DNA repair protein Length = 1309 Score = 29.1 bits (62), Expect = 3.7 Identities = 17/68 (25%), Positives = 33/68 (48%) Frame = +3 Query: 240 QRDAENVAAVADNPEASRSSNT*RSLKRPCKPTRTT*SRKSAGSTVSRSETVQTAPRVTG 419 Q+D E+ D E++R+ + ++ K+P S++SA ST +S + + Sbjct: 740 QQDPESTIQTQDQQESTRTEDVKKNRKKPTTSKPKKKSKESAKSTQKKSVDWDSLRKEAE 799 Query: 420 SSMRKKAK 443 S RK+ + Sbjct: 800 SGGRKRER 807 >At3g47290.1 68416.m05139 phosphoinositide-specific phospholipase C family protein similar to phosphoinositide-specific phospholipase C [Nicotiana rustica] GI:1771381, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase [Nicotiana rustica] GI:2765140; contains Pfam profiles PF00168: C2 domain, PF00388: Phosphatidylinositol-specific phospholipase C, X domain Length = 531 Score = 27.9 bits (59), Expect = 8.5 Identities = 19/73 (26%), Positives = 30/73 (41%), Gaps = 3/73 (4%) Frame = -2 Query: 211 RQRPRDPSYVAELSGHVA-PHGRRQASRNSESELIR--WWYSXXXXXXXXXXXXISRDNN 41 RQ P DP+Y + +S HV P G Q ++ I+ WY +R Sbjct: 271 RQHPADPNYQSLVSFHVVEPRGMLQNVLTGKANKIQRPGWYETDIISFTQKRFLRTRPQR 330 Query: 40 KYLRMSTERPSKS 2 K L + +P ++ Sbjct: 331 KLLIYAPYKPQRA 343 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,330,331 Number of Sequences: 28952 Number of extensions: 332988 Number of successful extensions: 782 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 767 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 782 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1863090400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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