BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021005 (734 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g28850.1 68418.m03549 calcium-binding EF hand family protein ... 29 2.4 At1g67500.1 68414.m07688 DNA polymerase family B protein similar... 28 7.4 At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 ... 27 9.8 At1g23540.1 68414.m02960 protein kinase family protein contains ... 27 9.8 >At5g28850.1 68418.m03549 calcium-binding EF hand family protein contains Pfam profile: PF00036 EF hand Length = 324 Score = 29.5 bits (63), Expect = 2.4 Identities = 25/93 (26%), Positives = 46/93 (49%), Gaps = 4/93 (4%) Frame = +1 Query: 466 IFTRRYSFLYFKYSQVD----MKKLLGGQIGLEDFIFAHRKGRPKEIEIVKTEDALGLTI 633 +++R YSFL+F Q D +K+LL GLE F+ + + + + E V + I Sbjct: 5 LYSRFYSFLFFFSWQDDFKPLLKELLATHPGLE-FLQSTPEFQERYAETVTYR--IFYYI 61 Query: 634 TDNGAGYAFIKRIKEGSIVXRIPHIEVGDHIEK 732 +G G + +K G+++ + H + + I K Sbjct: 62 NRSGNGRITFRELKRGNLIDAMLHADEEEDINK 94 >At1g67500.1 68414.m07688 DNA polymerase family B protein similar to SP|Q61493 DNA polymerase zeta catalytic subunit (EC 2.7.7.7) {Mus musculus}; contains Pfam profile PF00136: DNA polymerase family B Length = 1890 Score = 27.9 bits (59), Expect = 7.4 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +1 Query: 460 LRIFTRRYSFLYFK-YSQVDMKKLLGGQIGLEDFIFA 567 LR+F + + K Y K++L G++ L+DFIFA Sbjct: 1616 LRLFFEQKNISKVKSYLYRQWKRILSGRVSLQDFIFA 1652 >At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 subunit-related contains similarity to Swiss-Prot:P80387 5'-AMP-activated protein kinase, beta-1 subunit (AMPK beta-1 chain) (AMPKb) (40 kDa subunit) [Sus scrofa] Length = 591 Score = 27.5 bits (58), Expect = 9.8 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +1 Query: 598 IVKTEDALGLTITDNGAGYAFIKRIKEGSIVXRIPHIEVGDHIEK 732 +V E LG+ + G F+ IK+GS + I VGD ++K Sbjct: 78 MVTLEKPLGIRFALSADGKIFVHAIKKGSNAEKARIIMVGDTLKK 122 >At1g23540.1 68414.m02960 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 720 Score = 27.5 bits (58), Expect = 9.8 Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 1/57 (1%) Frame = +2 Query: 320 QST*VDNSNGTQKSQLVFHCQQAHGSPLGLISGFSNVKELYEKIAECYE-FSPEDIL 487 Q + + NS GT H Q+ G+P I G YE++AE + F+ ++IL Sbjct: 321 QQSSMGNSYGTAGGGYPHHQMQSSGTPDSAILGSGQTHFSYEELAEITQGFARKNIL 377 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,614,790 Number of Sequences: 28952 Number of extensions: 293188 Number of successful extensions: 699 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 691 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 699 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1614253080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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