Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0956
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 61 2e-10
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 55 8e-09
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 49 5e-07
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 44 2e-05
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 42 8e-05
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 34 0.022
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 29 0.46
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 29 0.46
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 28 1.4
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 27 3.3
SPAC3F10.02c |trk1|sptrk|potassium ion transporter Trk1|Schizosa... 26 5.7
SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1 |Schizosacc... 26 5.7
SPAC22F8.04 |||triose phosphate transporter |Schizosaccharomyces... 25 7.6
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 25 7.6
SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 25 7.6
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 25 7.6
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 10.0
SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 25 10.0
SPBC18E5.13 ||SPBC23G7.03|translation initiation factor |Schizos... 25 10.0
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 60.9 bits (141), Expect = 2e-10
Identities = 38/167 (22%), Positives = 77/167 (46%), Gaps = 8/167 (4%)
Frame = +1
Query: 199 LTFGEMTQQIVNIASAIKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGATVTFINSAYS 378
L+F ++ + + A A+++LG+ KGD + + +L T A L T+
Sbjct: 97 LSFNDIYDKALRYAGALRKLGLNKGDKFELYAPTSAFWLLTAEACLSQSMTIVTAYDTLG 156
Query: 379 KNEFIHTTKISKPKYIFLSPSAYKEFYVTLKKLNIVQKFFLYGISDDKDVISFRDL---- 546
+ +H+ + S + ++ K LK++ ++ + ++D+ + +++
Sbjct: 157 EEGLLHSLRESGVRGMYTEGHLLKTLVNPLKEIESLEVIIYRNDAKEEDIKTIQEIRPNL 216
Query: 547 -ATKYTHFNDFRP---KDFNGTDSTSIILYSSGTTGLPKGVKLTHRN 675
K+ F P D + I+Y+SG+TGLPKGV L+H+N
Sbjct: 217 KLIKFADFEKMSPPVEPDPPSPEEICCIMYTSGSTGLPKGVILSHKN 263
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 55.2 bits (127), Expect = 8e-09
Identities = 50/173 (28%), Positives = 77/173 (44%), Gaps = 4/173 (2%)
Frame = +1
Query: 169 ALINGASGEKLTFGEMTQQIVNIASAIKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGA 348
AL+ + +L+F E+ I+++ I LGI GD V I N E++ AV A
Sbjct: 18 ALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQRA 77
Query: 349 TVTFINSAYSKNEFIHTTKISKPKYIFL---SPSAYKEFYVTLKKLNI-VQKFFLYGISD 516
+NS Y ++EF K K + + S +A KKL++ V + S
Sbjct: 78 ICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVAANTPAVRAAKKLSVAVAELAWCPKSR 137
Query: 517 DKDVISFRDLATKYTHFNDFRPKDFNGTDSTSIILYSSGTTGLPKGVKLTHRN 675
++ F N +P D ++L++SGTTG PK V LTH+N
Sbjct: 138 LVRIVHFEG-----AKINAPQPLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKN 185
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 49.2 bits (112), Expect = 5e-07
Identities = 45/169 (26%), Positives = 76/169 (44%), Gaps = 13/169 (7%)
Frame = +1
Query: 202 TFGEMTQQIVNIASAIKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGATVTFINSAYSK 381
T+ ++ + +A + + GI +GD+V + + + + + VL GAT + I+ AY
Sbjct: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
Query: 382 NEFIHTTKISKPKYI-------FLSPSA--YKEFYVTLK----KLNIVQKFFLYGISDDK 522
I ++KP+ + LSP+ Y E + LK L + + L G S K
Sbjct: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
Query: 523 DVISFRDLATKYTHFNDFRPKDFNGTDSTSIILYSSGTTGLPKGVKLTH 669
D+ H + G DST + ++SG+ G+PKGVK H
Sbjct: 434 GA---DDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRH 479
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 44.4 bits (100), Expect = 2e-05
Identities = 41/160 (25%), Positives = 71/160 (44%)
Frame = +1
Query: 196 KLTFGEMTQQIVNIASAIKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGATVTFINSAY 375
+LT+ E+ ++ +A +K G G I+A+ + E + +A+L G ++ +
Sbjct: 2751 RLTYSELNERANKLAHNLKSYGFRVGSIIAVYFDKCIEAFISMLAILKAGCCFLALDVS- 2809
Query: 376 SKNEFIHTTKISKPKYIFLSPSAYKEFYVTLKKLNIVQKFFLYGISDDKDVISFRDLATK 555
+ E I + +S E Y L LN + SD + + ++
Sbjct: 2810 APTERIRYIVTDSTAVLVMSTG---ELYTKL--LNASINVTILDASDPGNYSN--NIENP 2862
Query: 556 YTHFNDFRPKDFNGTDSTSIILYSSGTTGLPKGVKLTHRN 675
YT KDF ++ + +LY+SG+TG PKG LTH N
Sbjct: 2863 YT-------KDFEDSN-LAYVLYTSGSTGKPKGCCLTHHN 2894
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/24 (58%), Positives = 20/24 (83%)
Frame = +1
Query: 601 DSTSIILYSSGTTGLPKGVKLTHR 672
DS + +LY+SG+TG PKGV ++HR
Sbjct: 359 DSVAYVLYTSGSTGNPKGVAISHR 382
Score = 26.2 bits (55), Expect = 4.3
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +1
Query: 247 IKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGA 348
+ +GI K D+VA+ + +Y + + VL GA
Sbjct: 1797 LHSIGIKKNDVVAVFIDKSLDYYSLMLGVLRIGA 1830
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 41.9 bits (94), Expect = 8e-05
Identities = 38/169 (22%), Positives = 72/169 (42%), Gaps = 13/169 (7%)
Frame = +1
Query: 190 GEKLTFGEMTQQIVNIASAIKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGATVTFINS 369
G +T+ E+ + A A++ +G+G GD VAI E + +A++ GA + I +
Sbjct: 115 GRYITYRELLASVSQCAGALQSMGVGMGDRVAIYMPMIPETIIAMLAIVRLGAIHSVIFA 174
Query: 370 AYSKNEFIHTTKISKPKYIFLSPSAYK---------EFYVTLKKLNIVQKFFLYGISDD- 519
+S S+ K I + +++ L + ++K ++ S +
Sbjct: 175 GFSAESVADRVNDSECKVIITADESHRGGKRIPLKGVVNKALTECPTIKKVLVFQRSAEP 234
Query: 520 -KDVISFRDL--ATKYTHFNDFRPKDFNGTDSTSIILYSSGTTGLPKGV 657
++ RD+ F + P + +LY+SG+TG PKGV
Sbjct: 235 TASMVEGRDVWWHDIIPKFPRYCPPAVVNPEHPLFLLYTSGSTGKPKGV 283
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 33.9 bits (74), Expect = 0.022
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = +1
Query: 601 DSTSIILYSSGTTGLPKGVKLTHRN 675
D +Y+SG+TG PKGV L HRN
Sbjct: 245 DDICCYMYTSGSTGKPKGVVLLHRN 269
>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 695
Score = 29.5 bits (63), Expect = 0.46
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = -2
Query: 471 LQSYIKLLVSTWRQEDVFGFRYFSCMNEFIFAICTVYESNRSSCTKHSYSCSQILCSVFT 292
L S +K + W + F Y S + +F I T S+R + KHS + ++FT
Sbjct: 325 LSSVLKSTKTKWYDFETFSNSYSSTIIDFPLGIFTTNASSRDNFLKHSSLFQVMNTAIFT 384
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 29.5 bits (63), Expect = 0.46
Identities = 16/62 (25%), Positives = 31/62 (50%)
Frame = -2
Query: 225 LLCHFAKCQFLSASSIDQSHFCFFFRKSLQ*KFSIIHVIRKRKILLSINIPAFCFSFSIL 46
LL + +K ++++ ID++ F FF K ++ S + +++ I CF FS +
Sbjct: 81 LLLYKSKYEYITWDLIDENRFQLFFEKVIEVSLS----LNLSEVVYMIQFITLCFQFSNI 136
Query: 45 HK 40
K
Sbjct: 137 EK 138
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 27.9 bits (59), Expect = 1.4
Identities = 23/83 (27%), Positives = 38/83 (45%), Gaps = 6/83 (7%)
Frame = +1
Query: 22 IRHEETLVQNG-----KRKAKCGYIYGEQNFSFPNHMNYGKFLL-ERLSKEKAKVALING 183
+ E+T++ G K +C I G + PN Y K L ERL+K +A+I
Sbjct: 356 VTKEDTIIMKGAGDHVKVNDRCEQIRGVM--ADPNLTEYEKEKLQERLAKLSGGIAVIKV 413
Query: 184 ASGEKLTFGEMTQQIVNIASAIK 252
++ E +IV+ +A+K
Sbjct: 414 GGSSEVEVNEKKDRIVDALNAVK 436
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 26.6 bits (56), Expect = 3.3
Identities = 21/71 (29%), Positives = 34/71 (47%)
Frame = -2
Query: 252 FNSRSYIYNLLCHFAKCQFLSASSIDQSHFCFFFRKSLQ*KFSIIHVIRKRKILLSINIP 73
F+ S + + HF QF S S I +H C RK +I +++ + L+ ++ P
Sbjct: 264 FSGDSSLKSFSLHFFALQFFSTSLIQYTHIC---RK------CVITILQSYQQLI-VDQP 313
Query: 72 AFCFSFSILHK 40
A FS+L K
Sbjct: 314 ANLLKFSLLSK 324
>SPAC3F10.02c |trk1|sptrk|potassium ion transporter
Trk1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 841
Score = 25.8 bits (54), Expect = 5.7
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 3/42 (7%)
Frame = +1
Query: 397 TTKISKPKYIFLSPSAYKEF---YVTLKKLNIVQKFFLYGIS 513
TTKI +FL+ SA + V L L+I Q+F LYG +
Sbjct: 44 TTKIKYIDALFLASSATTQTGLNSVDLNSLSIWQQFILYGFT 85
>SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 25.8 bits (54), Expect = 5.7
Identities = 12/49 (24%), Positives = 25/49 (51%)
Frame = +1
Query: 433 SPSAYKEFYVTLKKLNIVQKFFLYGISDDKDVISFRDLATKYTHFNDFR 579
+PS + F V +++LN+V LY S + ++D + H + ++
Sbjct: 155 NPSLHDNFSVRIQELNVVDIAMLYNSSRPSLAVLYKD-SKSIVHLSTYK 202
>SPAC22F8.04 |||triose phosphate transporter |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 383
Score = 25.4 bits (53), Expect = 7.6
Identities = 15/48 (31%), Positives = 21/48 (43%)
Frame = -2
Query: 171 SHFCFFFRKSLQ*KFSIIHVIRKRKILLSINIPAFCFSFSILHKCFLV 28
S FF S Q F+I+ I ++ L IN P SF + +V
Sbjct: 85 SRLTIFFAVSSQIVFAILVTILNKQALNIINAPLLMLSFQMAFTSLMV 132
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.4 bits (53), Expect = 7.6
Identities = 20/57 (35%), Positives = 27/57 (47%)
Frame = -2
Query: 369 TVYESNRSSCTKHSYSCSQILCSVFTANSYYIAFPNAKLFNSRSYIYNLLCHFAKCQ 199
T Y+ N S + SCS+ +CS FT Y F + N R IY L F+ C+
Sbjct: 814 TNYDVNEVSDSDSKLSCSKYMCS-FT--FIYSDFTSNITNNYRPRIY-YLAQFSVCE 866
>SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1250
Score = 25.4 bits (53), Expect = 7.6
Identities = 17/46 (36%), Positives = 24/46 (52%)
Frame = -3
Query: 641 PVVPEE*RIIDVLSVPLKSFGLKSLK*VYFVARSLKLMTSLSSDIP 504
P+VPE I+D + L + G+ Y + LKL TS SD+P
Sbjct: 13 PLVPES--IVDAPTQRLYAIGVFVALQAYKIYDLLKLETSSISDVP 56
>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 619
Score = 25.4 bits (53), Expect = 7.6
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 297 KQNRVSDYNYSCALYRSY 350
+QN SDY+ C LY++Y
Sbjct: 529 QQNGYSDYDRCCPLYKTY 546
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.0 bits (52), Expect = 10.0
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +3
Query: 198 IDIWRNDTANCKYSF 242
+DIWRND N K+ +
Sbjct: 1334 LDIWRNDQPNSKFVY 1348
>SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1125
Score = 25.0 bits (52), Expect = 10.0
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +1
Query: 409 SKPKYIFLSPSAYKEFYVTLKKLN 480
S+ K++ LSP K++Y KLN
Sbjct: 1050 SQQKFLSLSPQVSKDYYDVRSKLN 1073
>SPBC18E5.13 ||SPBC23G7.03|translation initiation factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 233
Score = 25.0 bits (52), Expect = 10.0
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 279 YIAFPNAKLFNSRSYIYNLLCHFAKCQ 199
Y+ FP+ KLF + Y+L F KCQ
Sbjct: 4 YLQFPHRKLF--IQFSYSLTSVFRKCQ 28
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,993,981
Number of Sequences: 5004
Number of extensions: 67002
Number of successful extensions: 242
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 239
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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