Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0927
(715 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase |Schizosacchar... 130 2e-31
SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c... 110 2e-25
SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyce... 56 5e-09
SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 39 6e-04
SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 31 0.16
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 28 1.5
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 28 1.5
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 27 2.7
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 27 3.5
SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch... 26 4.7
SPAC222.14c |||GTP binding protein Sey1 |Schizosaccharomyces pom... 26 6.1
SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po... 26 6.1
SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|ch... 25 8.1
>SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 390
Score = 130 bits (314), Expect = 2e-31
Identities = 73/192 (38%), Positives = 110/192 (57%), Gaps = 2/192 (1%)
Frame = +2
Query: 143 TIAIHAGQEPEKWNSAAVVTPIVTSTTFKQPAPAEHTG-FEYGRSGNPTRNTLEECLAAL 319
T +H +++++++V PI S TFKQP EH G F+Y RSGNPTR+ L+ LA L
Sbjct: 11 TELVHVEGNEDQYHASSV--PIYQSATFKQPC-LEHMGKFDYTRSGNPTRSVLQVHLAKL 67
Query: 320 DGGKYGLTFASXXXXXXXXXXXXNHGDHIVSSDDVYGGTNRLLRQVIARLGINTTFTDFT 499
K+ ++ H+V+ D+YGG++RLL + G D +
Sbjct: 68 MKAKHAFVTSNGMSALDMILRCCKSNSHVVAGHDLYGGSDRLLSFNQRQYGFKVDNVDTS 127
Query: 500 NIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIVKIARSRG-DIIVVVDNTFLTPYLQR 676
++ + A++ +T ++ IE+PTNP + + DI AIVKI RS+ D ++V+DNT L+P L
Sbjct: 128 DLAAFEAALRPDTNLVLIESPTNPRISICDIRAIVKITRSKAKDALLVMDNTMLSPVLCN 187
Query: 677 PLDFGADIVMYS 712
PLDFG DIV S
Sbjct: 188 PLDFGYDIVYES 199
>SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 429
Score = 110 bits (264), Expect = 2e-25
Identities = 70/202 (34%), Positives = 108/202 (53%), Gaps = 10/202 (4%)
Frame = +2
Query: 137 FATIAIHAGQEPEKWNSAAVVTPIVTSTTFK--------QPAPAEHTGFEYGRSGNPTRN 292
F T+ +HAGQEP+ S+ V PI +T++ + + G+ Y R NPT +
Sbjct: 8 FETLQLHAGQEPDAATSSRAV-PIYATTSYVFRDCDHGGRLFGLQEPGYIYSRMMNPTAD 66
Query: 293 TLEECLAALDGGKYGL-TFASXXXXXXXXXXXXNHGDHIVSSDDVYGGTNRLLRQVIARL 469
E+ +AAL+ G + T + GD+IVS+ +YGGT L + + RL
Sbjct: 67 VFEKRIAALEHGAAAIATSSGTSALFMALTTLAKAGDNIVSTSYLYGGTYNLFKVTLPRL 126
Query: 470 GINTTFTDFTNIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIVKIARSRGDIIVVVDN 649
GI T F + + + + ENTK +++E+ NP+ V D E I ++A + G + ++VDN
Sbjct: 127 GITTKFVNGDDPNDLAAQIDENTKAVYVESIGNPMYNVPDFERIAEVAHAAG-VPLMVDN 185
Query: 650 TFLTP-YLQRPLDFGADIVMYS 712
TF YL RP+D GADIV +S
Sbjct: 186 TFGGGGYLVRPIDHGADIVTHS 207
>SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 398
Score = 56.0 bits (129), Expect = 5e-09
Identities = 35/96 (36%), Positives = 56/96 (58%)
Frame = +2
Query: 425 YGGTNRLLRQVIARLGINTTFTDFTNIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIV 604
Y GT +++ ++ G+ T+F I+ +A+ E +IW+ETP NPL DI
Sbjct: 121 YSGTIQIIARINRLTGLETSF-----IDGKCDAIGEGD-VIWLETPLNPLGIAFDIPFYK 174
Query: 605 KIARSRGDIIVVVDNTFLTPYLQRPLDFGADIVMYS 712
++A+ +G I+VVD+TF P +Q L GAD V++S
Sbjct: 175 ELAKKKG-AILVVDSTFAPPPIQDALVLGADYVVHS 209
>SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 421
Score = 39.1 bits (87), Expect = 6e-04
Identities = 21/54 (38%), Positives = 34/54 (62%), Gaps = 2/54 (3%)
Frame = +2
Query: 500 NIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIVKIAR--SRGDIIVVVDNTF 655
++ K++NA+ E TKMI I TP NPL K+ E + +IA + +++VV D +
Sbjct: 166 DMNKLRNAITEKTKMIVINTPHNPLGKIFSEEELNEIADLVLKHNLLVVSDEVY 219
>SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 610
Score = 31.1 bits (67), Expect = 0.16
Identities = 18/41 (43%), Positives = 23/41 (56%)
Frame = +2
Query: 530 ENTKMIWIETPTNPLLKVVDIEAIVKIARSRGDIIVVVDNT 652
E ++ E P+NPLL D+ I K+A S D VVVD T
Sbjct: 366 EKVMALFCEFPSNPLLNSPDLVRIRKLADSY-DFAVVVDET 405
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 27.9 bits (59), Expect = 1.5
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2
Query: 462 AITCLNSLFVPPYTSSED 409
A TC N L +PPYTS +D
Sbjct: 732 AHTCFNRLDLPPYTSKKD 749
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +2
Query: 479 TTFTDFTNIEKVKNAMQENTKMIWIETPTNPLLKVV 586
T + D N EK + +NT+ ++T NPLL ++
Sbjct: 22 TIYIDLLNYEKQLRNLNKNTREDSLQTNLNPLLSLI 57
>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1367
Score = 27.1 bits (57), Expect = 2.7
Identities = 10/34 (29%), Positives = 18/34 (52%)
Frame = -2
Query: 531 SCMAFFTFSIFVKSVNVVLIPNLAITCLNSLFVP 430
+C F + + V ++ + L+P + CL LF P
Sbjct: 1277 TCQTFGFWCVTVLTIALCLLPRFSYICLQKLFYP 1310
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 462 AITCLNSLFVPPYTSSED 409
A TC N L +PPY S E+
Sbjct: 636 AHTCFNRLDIPPYNSKEE 653
>SPAC4F10.18 |||WD repeat protein, human NUP37
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +2
Query: 419 DVYGGTNRLLRQVIARLGINTT 484
DVY NRL QVIA +G + T
Sbjct: 144 DVYSADNRLAEQVIASVGDDCT 165
>SPAC222.14c |||GTP binding protein Sey1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 762
Score = 25.8 bits (54), Expect = 6.1
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +2
Query: 473 INTTF-TDFTNIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIVKIARSRGD 628
+N F T F+ ++ K Q+ TK IW+ N + V+D+E R RG+
Sbjct: 56 LNNLFGTSFSVMDASKR--QQTTKGIWLSKANNSPILVMDVEG--TDGRERGE 104
>SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1564
Score = 25.8 bits (54), Expect = 6.1
Identities = 16/51 (31%), Positives = 22/51 (43%)
Frame = +2
Query: 194 VVTPIVTSTTFKQPAPAEHTGFEYGRSGNPTRNTLEECLAALDGGKYGLTF 346
V + ++ TTF Q P+ +E N T N L E +L YG F
Sbjct: 479 VSSELLHMTTFTQSLPSGFKAYEIIPEPNVTGNALIELQESLHLDSYGFFF 529
>SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 333
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +3
Query: 162 AKNRKNGIQQPWLHPLSHPLHSNSQH 239
+KN +N Q P +P H + SQH
Sbjct: 16 SKNSQNASQPPETYPFYHEVRQMSQH 41
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,039,341
Number of Sequences: 5004
Number of extensions: 66196
Number of successful extensions: 227
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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