Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0875
(638 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 141 9e-35
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 140 2e-34
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 28 0.99
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch... 28 0.99
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 25 7.0
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 7.0
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 25 9.2
SPBC337.16 |cho1||phosphatidyl-N-methylethanolamine N-methyltran... 25 9.2
SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 25 9.2
SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyc... 25 9.2
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 25 9.2
SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces ... 25 9.2
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 141 bits (341), Expect = 9e-35
Identities = 73/151 (48%), Positives = 98/151 (64%), Gaps = 2/151 (1%)
Frame = +3
Query: 12 GPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFG 191
GPL++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+SAFG LD +FG
Sbjct: 206 GPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFG 265
Query: 192 SWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNK 365
S ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV RA +K NPL N
Sbjct: 266 STTEAAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKA 325
Query: 366 AMLKLNPYAAVLKRKAILELRRRKNLKALAD 458
+ +LNPYA KA ++L K KA +
Sbjct: 326 VLARLNPYAKAY--KANVKLNTGKTPKAAGE 354
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 140 bits (339), Expect = 2e-34
Identities = 72/151 (47%), Positives = 98/151 (64%), Gaps = 2/151 (1%)
Frame = +3
Query: 12 GPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFG 191
GPL++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+SAFG LD +FG
Sbjct: 206 GPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFG 265
Query: 192 SWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNK 365
S ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV RA +K NPL N
Sbjct: 266 STTEVAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKA 325
Query: 366 AMLKLNPYAAVLKRKAILELRRRKNLKALAD 458
+ +LNPYA KA +++ K KA +
Sbjct: 326 VLSRLNPYAKAY--KANVKINSEKTPKAAGE 354
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 28.3 bits (60), Expect = 0.99
Identities = 27/109 (24%), Positives = 46/109 (42%)
Frame = +3
Query: 210 KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 389
KQK + + Q K LT+ S E + VL +R T+K LTN+ ++K +P
Sbjct: 730 KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPT 787
Query: 390 AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRERRRK 536
+ ++ + + L ++ L+KRN K + RK
Sbjct: 788 PQITVKQNTTKSSSAPRMGMLEQLKQE--YLTKRNFESKLKSSAVSSRK 834
>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 28.3 bits (60), Expect = 0.99
Identities = 19/90 (21%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
Frame = +3
Query: 276 KSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKA-- 449
+S + + +K+ IR R+L+P ++ + N Y+ + + ++ R R
Sbjct: 659 RSRSVTPINNINHKKYIRKDRELSPRGRERSSNR-NSYSDLSRSSSLSRGRSRSYTPEGR 717
Query: 450 LADAEKSGLKLSKRNPAMKAEKLRERRRKN 539
L ++E G + +PA + + R+R R++
Sbjct: 718 LIESEDKGYRSRSSSPASRKYRSRQRYRRS 747
>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 500
Score = 25.4 bits (53), Expect = 7.0
Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 1/93 (1%)
Frame = +3
Query: 258 DLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAM-LKLNPYAAVLKRKAILELRRR 434
++T LK DE+RK+ N + L + +++ + L+ + K + +EL
Sbjct: 366 EVTETLKGDELRKISLQVNVKFSEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMEL--- 422
Query: 435 KNLKALADAEKSGLKLSKRNPAMKAEKLRERRR 533
N LA+ E LKL + + R+RRR
Sbjct: 423 -NKDYLAEEEAKNLKLQEDLKKGIVRQPRKRRR 454
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 25.4 bits (53), Expect = 7.0
Identities = 17/54 (31%), Positives = 32/54 (59%)
Frame = -1
Query: 392 RVRIEFQHRLVVSERVQFACSTDHAFVGSTEDLPDLIRLEKTCEVSVGHLRLGQ 231
+V +EF+ RL + +RV+ AF+GS E + L+ + +T + ++ L LG+
Sbjct: 207 QVAVEFRKRLNIGDRVKDGLLYKDAFLGS-EAVDVLMHIVRTTDRNLA-LLLGR 258
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit
Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 25.0 bits (52), Expect = 9.2
Identities = 20/70 (28%), Positives = 36/70 (51%)
Frame = +3
Query: 324 IRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAM 503
I+ +K+N L + L+ ++ L+ K LR+ L+ + D +K KLSK M
Sbjct: 865 IKLMKKVNILESRLLSNPLHNFSE-LEEKYAEYLRKLALLEEVKDLKK---KLSKARSIM 920
Query: 504 KAEKLRERRR 533
+ ++L R+R
Sbjct: 921 QLDELNSRKR 930
>SPBC337.16 |cho1||phosphatidyl-N-methylethanolamine
N-methyltransferase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 221
Score = 25.0 bits (52), Expect = 9.2
Identities = 16/58 (27%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = -2
Query: 502 IAGFRLD-SFNPLFSASARAFKFFLLRSSKIAFLFSTAA*GLSFSIALLLVSGFNLRV 332
++GF + + NP+++ S F LR K+A L T + IAL F ++
Sbjct: 151 VSGFPFNVNDNPMYNGSTLCFLSTALRYGKVAGLLLTLEVFFVYRIALKFEEPFTAKI 208
>SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 497
Score = 25.0 bits (52), Expect = 9.2
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +1
Query: 139 VSSSGLSPHSAGLTPYSGHGRHHQNK 216
VS S HS P+S GRH++ K
Sbjct: 120 VSRSSSIGHSGSTAPWSSVGRHNRKK 145
>SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 430
Score = 25.0 bits (52), Expect = 9.2
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = +1
Query: 193 HGRHHQNKRRTSTCPNRRW 249
HG H S CPN RW
Sbjct: 378 HGGHTNRISEFSWCPNERW 396
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.0 bits (52), Expect = 9.2
Identities = 24/96 (25%), Positives = 41/96 (42%), Gaps = 5/96 (5%)
Frame = +3
Query: 198 KTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLK 377
KTPS +K+ P P + N D L ++ V + P+ + TR + P ++ +
Sbjct: 586 KTPSPEKQKVLSPPPIITNFDKETLASNEAHEAVPQKPS--APQVTRLMAPQDSSSVVTP 643
Query: 378 -----LNPYAAVLKRKAILELRRRKNLKALADAEKS 470
L+P AV K ++ + A AD E +
Sbjct: 644 SPTSLLDPARAVRKVIDGIDPPKEAGAGATADVESA 679
>SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 607
Score = 25.0 bits (52), Expect = 9.2
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +3
Query: 171 RLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLN 347
RL+ +W ++ +PQ K + + LK DEIR+ V+ + +LN
Sbjct: 259 RLESAKTNWVYAKGERFAVAVPQSKQVSFATKKELKKDEIRRSYSYGGSSVVFGSDELN 317
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,110,805
Number of Sequences: 5004
Number of extensions: 38499
Number of successful extensions: 136
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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