Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0677
(663 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 89 2e-18
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 87 7e-18
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 85 4e-17
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 83 2e-16
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 81 9e-16
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 79 3e-15
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 77 1e-14
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 59 3e-09
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 51 8e-07
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 48 7e-06
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 44 1e-04
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 33 0.17
At1g17580.1 68414.m02165 myosin, putative similar to myosin GI:4... 30 1.6
At5g03830.1 68418.m00352 expressed protein hypothetical protein ... 29 2.1
At2g32350.1 68415.m03954 ubiquitin family protein contains INTER... 29 2.1
At1g04160.1 68414.m00406 myosin family protein contains Pfam pro... 29 2.8
At5g43900.1 68418.m05368 myosin heavy chain (MYA2) nearly identi... 29 3.6
At1g22210.1 68414.m02777 trehalose-6-phosphate phosphatase, puta... 29 3.6
At4g09440.1 68417.m01554 hypothetical protein contains Pfam PF04... 28 4.8
At4g39660.1 68417.m05608 alanine--glyoxylate aminotransferase, p... 28 6.4
At3g02500.1 68416.m00238 expressed protein 28 6.4
At5g03000.1 68418.m00245 kelch repeat-containing F-box family pr... 27 8.4
At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 27 8.4
At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50... 27 8.4
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 89.4 bits (212), Expect = 2e-18
Identities = 37/68 (54%), Positives = 48/68 (70%)
Frame = +3
Query: 24 EHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLKK 203
EH WI+I+ VY+VTKFLE+HPGG D LL GKD T FEDVGHS+ ARE++++
Sbjct: 15 EHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQ 74
Query: 204 YKIGTLPP 227
Y +G + P
Sbjct: 75 YYVGEIDP 82
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 87.4 bits (207), Expect = 7e-18
Identities = 37/65 (56%), Positives = 46/65 (70%)
Frame = +3
Query: 27 HKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLKKY 206
H + W++I VYDV+ +++EHPGG D LL VAGKD T FED GHS DAREL++KY
Sbjct: 16 HNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKDARELMEKY 75
Query: 207 KIGTL 221
IG L
Sbjct: 76 FIGEL 80
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 85.0 bits (201), Expect = 4e-17
Identities = 36/64 (56%), Positives = 47/64 (73%)
Frame = +3
Query: 24 EHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLKK 203
+HK + WI+IH VYD++ F++EHPGG + LL V GKD + FEDV HS DA+EL+KK
Sbjct: 12 KHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAKELMKK 71
Query: 204 YKIG 215
Y IG
Sbjct: 72 YCIG 75
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 83.0 bits (196), Expect = 2e-16
Identities = 35/66 (53%), Positives = 45/66 (68%)
Frame = +3
Query: 24 EHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLKK 203
+H K W+II VYDVT F+++HPGG + LL GKD T FEDVGHSD AR+++ K
Sbjct: 15 KHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDMMDK 74
Query: 204 YKIGTL 221
Y IG +
Sbjct: 75 YFIGEI 80
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 80.6 bits (190), Expect = 9e-16
Identities = 33/66 (50%), Positives = 44/66 (66%)
Frame = +3
Query: 24 EHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLKK 203
+H K WI+I VYDVTKFL++HPGG + +L GKD T FEDVGHS A+ +L +
Sbjct: 15 QHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSSTAKAMLDE 74
Query: 204 YKIGTL 221
Y +G +
Sbjct: 75 YYVGDI 80
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 78.6 bits (185), Expect = 3e-15
Identities = 35/71 (49%), Positives = 48/71 (67%)
Frame = +3
Query: 9 IRHEEEHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDAR 188
I +H S WII+H +YD T+FL++HPGG DS+L AG D T+ FE + HSD A+
Sbjct: 550 ISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-HSDKAK 608
Query: 189 ELLKKYKIGTL 221
+LL+ Y+IG L
Sbjct: 609 KLLEDYRIGEL 619
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 77.0 bits (181), Expect = 1e-14
Identities = 33/67 (49%), Positives = 48/67 (71%)
Frame = +3
Query: 21 EEHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLK 200
++H S WII+H +YD T+FL +HPGG+DS+L AG D T+ FE + HSD A+++L+
Sbjct: 551 KKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAI-HSDKAKKMLE 609
Query: 201 KYKIGTL 221
Y+IG L
Sbjct: 610 DYRIGEL 616
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 58.8 bits (136), Expect = 3e-09
Identities = 26/65 (40%), Positives = 39/65 (60%)
Frame = +3
Query: 27 HKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLKKY 206
H WIII + VYD+T ++EEHPGG D++L+ AG D T F H+ ++++ +
Sbjct: 57 HNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTDGFFGPQHATRVFDMIEDF 115
Query: 207 KIGTL 221
IG L
Sbjct: 116 YIGEL 120
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 50.8 bits (116), Expect = 8e-07
Identities = 21/67 (31%), Positives = 38/67 (56%)
Frame = +3
Query: 21 EEHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDARELLK 200
++H+ S+W ++ VY+++ ++ HPGG D L++ G+DGT F + LL+
Sbjct: 135 KKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNVDILLE 194
Query: 201 KYKIGTL 221
K +G L
Sbjct: 195 KCLVGVL 201
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 47.6 bits (108), Expect = 7e-06
Identities = 19/46 (41%), Positives = 30/46 (65%)
Frame = +3
Query: 21 EEHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAF 158
++H +WI I VYDV+ +++ HPGG ++L +AG+D T AF
Sbjct: 16 KKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 43.6 bits (98), Expect = 1e-04
Identities = 17/46 (36%), Positives = 28/46 (60%)
Frame = +3
Query: 21 EEHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAF 158
++H +WI I VY+V+ +++ HPGG +L + G+D T AF
Sbjct: 16 KKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 33.1 bits (72), Expect = 0.17
Identities = 23/58 (39%), Positives = 29/58 (50%)
Frame = -3
Query: 265 NFEQSVMILHLSPGGNVPILYFFNNSRASSLCPTSSKAWVPSFPATSNNESAPPGCSS 92
N ILHLS + IL F S +SSL P+SS + P +S+ SAPP S
Sbjct: 2 NILSQTQILHLS----IAILLFITTS-SSSLSPSSSSPSLSPSPPSSSPSSAPPSSLS 54
>At1g17580.1 68414.m02165 myosin, putative similar to myosin
GI:433663 from (Arabidopsis thaliana)
Length = 1520
Score = 29.9 bits (64), Expect = 1.6
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 18 EEEHKDEKSVWIIIHN-DVYDVTKFLEEHPGGADSLLEVA 134
+EE+ E+ W I D DV + +E+ PGG SLL+ A
Sbjct: 468 QEEYTKEEIAWSYIEFIDNQDVLELIEKKPGGIISLLDEA 507
>At5g03830.1 68418.m00352 expressed protein hypothetical protein
F4I1.32, Arabidopsis thaliana, PIR:T02406
Length = 265
Score = 29.5 bits (63), Expect = 2.1
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -2
Query: 89 KLSHVVNIIMNDYPHALFVFMFFLVPNSC 3
K SH+V ++ ND LFVF L+ N C
Sbjct: 113 KASHLVEVL-NDVQRCLFVFAIMLMENKC 140
>At2g32350.1 68415.m03954 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 233
Score = 29.5 bits (63), Expect = 2.1
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +3
Query: 345 KRNTFYANPIDLKSDYQDVNEYGTILERFEILL 443
KR Y + I+L DY+++NEYG I E EI++
Sbjct: 112 KRMQLYYSGIELADDYRNLNEYG-ITEFSEIVV 143
>At1g04160.1 68414.m00406 myosin family protein contains Pfam
profiles: PF02736 myosin N-terminal SH3-like domain,
PF00063 myosin head (motor domain), PF00612 IQ
calmodulin-binding motif, PF01843: DIL domain
Length = 1500
Score = 29.1 bits (62), Expect = 2.8
Identities = 21/65 (32%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Frame = +3
Query: 18 EEEHKDEKSVWIIIHN-DVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDDAREL 194
+EE+K E+ W I D D+ +E+ PGG +LL D F H A++L
Sbjct: 472 QEEYKKEEINWSYIEFVDNQDILDLIEKKPGGIIALL-----DEACMFPRSTHETFAQKL 526
Query: 195 LKKYK 209
+ YK
Sbjct: 527 YQTYK 531
>At5g43900.1 68418.m05368 myosin heavy chain (MYA2) nearly identical
to PIR|S51824 myosin heavy chain MYA2 [Arabidopsis
thaliana]
Length = 1505
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 18 EEEHKDEKSVWIIIHN-DVYDVTKFLEEHPGGADSLLEVA 134
+EE+K E+ W I D D+ +E+ PGG +LL+ A
Sbjct: 471 QEEYKKEEINWSYIEFVDNQDILDLIEKKPGGIIALLDEA 510
>At1g22210.1 68414.m02777 trehalose-6-phosphate phosphatase,
putative similar to trehalose-6-phosphate phosphatase
(AtTPPB) GI:2944180 from [Arabidopsis thaliana];
contains Pfam profile PF02358: Trehalose-phosphatase
Length = 320
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 5/68 (7%)
Frame = +3
Query: 15 HEEEHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGH-----SD 179
H E+ DE+S WI +H D+ + + G ++ DGT + H +D
Sbjct: 33 HVSENDDERSSWIALHPSALDMFEQIMRDAEG-KQIIMFLDYDGTLSLITEDHDRAYITD 91
Query: 180 DARELLKK 203
+ RE++K+
Sbjct: 92 EMREVVKE 99
>At4g09440.1 68417.m01554 hypothetical protein contains Pfam PF04510
: Family of unknown function (DUF577); common family
comprised of At4g09440, At1g66000, At1g66060
Length = 177
Score = 28.3 bits (60), Expect = 4.8
Identities = 13/47 (27%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = +3
Query: 36 EKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGK--DGTQAFEDVG 170
+ S W++ + T L E P A+S+ E+A K D + ++G
Sbjct: 103 DNSCWVLAFTGAFCATIHLREFPSQAESVKEIANKMIDSVRELVEIG 149
>At4g39660.1 68417.m05608 alanine--glyoxylate aminotransferase,
putative / beta-alanine-pyruvate aminotransferase,
putative / AGT, putative similar to SP|Q64565
Alanine--glyoxylate aminotransferase 2, mitochondrial
precursor (EC 2.6.1.44) (AGT 2) (Beta-alanine-pyruvate
aminotransferase) {Rattus norvegicus}; contains Pfam
profile PF00202: aminotransferase, class III
Length = 476
Score = 27.9 bits (59), Expect = 6.4
Identities = 11/32 (34%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = -3
Query: 565 SVKLITQWYASSPGAATSPPNHPSLTHE-RPY 473
++ L+ +++SP A +PP+ P H+ RPY
Sbjct: 20 AISLLRTDFSTSPSIADAPPHIPPFVHQPRPY 51
>At3g02500.1 68416.m00238 expressed protein
Length = 278
Score = 27.9 bits (59), Expect = 6.4
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +3
Query: 465 FNM*GRSWVRLGWLGGEVAAPGLDAYHCVISFTENHMYVTSSVFTSAHGWQP 620
FN+ R W R G L E + + C+ SF E+H T++V S+ P
Sbjct: 10 FNL--REWARQGHLTREDQSSRRFSASCIRSFREDHKSCTTNVTISSTASSP 59
>At5g03000.1 68418.m00245 kelch repeat-containing F-box family
protein similar to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 354
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +3
Query: 516 VAAPGLDAYHCVISFTENHMYV 581
+A+P L+A I TENH+YV
Sbjct: 74 IASPDLEATRSRIGVTENHLYV 95
>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
thaliana]; identical to cDNA SMT3 protein GI:1707371
Length = 100
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 105 GGADSLLEVAGKDGTQAFEDVGHSDDARELLKKY 206
GGA L+V G+DG + F + S ++L+ Y
Sbjct: 14 GGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAY 47
>At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50)
identical to DNA repair-recombination protein GI:7110148
from [Arabidopsis thaliana]
Length = 1316
Score = 27.5 bits (58), Expect = 8.4
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = -2
Query: 311 DPYEQGSSQRHHCPFQFRTI-CDDFASFTWRQR 216
+P+E+ + Q H CP R+ D+ ASF +QR
Sbjct: 683 EPFEKRARQEHSCPCCERSFTADEEASFIKKQR 715
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,657,335
Number of Sequences: 28952
Number of extensions: 345523
Number of successful extensions: 1207
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1203
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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