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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0666
         (654 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g05140.1 68418.m00545 transcription elongation factor-related...    46   2e-05
At3g10820.1 68416.m01303 transcription elongation factor-related...    42   3e-04
At5g25520.2 68418.m03037 transcription elongation factor-related...    33   0.17 
At5g25520.1 68418.m03036 transcription elongation factor-related...    33   0.17 
At2g38560.1 68415.m04737 transcription factor S-II (TFIIS) domai...    33   0.22 
At5g11430.1 68418.m01335 transcription elongation factor-related...    32   0.38 
At2g25640.1 68415.m03073 transcription elongation factor-related...    31   0.51 

>At5g05140.1 68418.m00545 transcription elongation factor-related
           low similarity to transcription elongation factor
           TFIIS.h [Mus musculus] GI:3288547, elongin A [Mus
           musculus] GI:6009624
          Length = 436

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 24/51 (47%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
 Frame = +3

Query: 162 MAINLDVLTKTRIGMTVNALRKSSKDDEVISLCKTLIKNWKKFLST-PNTT 311
           M++N+D+L  T IG  VN LRK S  D++  L KTLI  WK+ +    NTT
Sbjct: 169 MSLNVDILKSTEIGKAVNGLRKHS-SDKIRQLAKTLIAEWKELVDQWVNTT 218


>At3g10820.1 68416.m01303 transcription elongation factor-related
           low similarity to transcription elongation factor
           TFIIS.h [Mus musculus] GI:3288547
          Length = 416

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 26/81 (32%), Positives = 45/81 (55%)
 Frame = +3

Query: 51  MSVEEDVMKIQKKLTKMTSEDGTGQXXXXXXXXXXQTMAINLDVLTKTRIGMTVNALRKS 230
           +SV+E VM+I+  L+   ++D              ++M++++D+L  T IG  VN LR+ 
Sbjct: 105 VSVDE-VMRIRDILS---NKDDEKDSVLLESLRKLESMSMSVDILKDTEIGKAVNGLRRH 160

Query: 231 SKDDEVISLCKTLIKNWKKFL 293
           S D ++  L KTL   WK+ +
Sbjct: 161 SSD-KISKLAKTLFAEWKRLV 180


>At5g25520.2 68418.m03037 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 997

 Score = 33.1 bits (72), Expect = 0.17
 Identities = 17/57 (29%), Positives = 26/57 (45%)
 Frame = +3

Query: 480 ETSNACGSPXXXXXXXXXCIYAEFKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNG 650
           ET  +   P          ++  F   + +YK + RS + NLKD  NP LR +  +G
Sbjct: 352 ETQKSFEDPELLASKIELELFKLFGGVNKKYKEKGRSLLFNLKDKNNPELRESVMSG 408


>At5g25520.1 68418.m03036 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 735

 Score = 33.1 bits (72), Expect = 0.17
 Identities = 17/57 (29%), Positives = 26/57 (45%)
 Frame = +3

Query: 480 ETSNACGSPXXXXXXXXXCIYAEFKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNG 650
           ET  +   P          ++  F   + +YK + RS + NLKD  NP LR +  +G
Sbjct: 352 ETQKSFEDPELLASKIELELFKLFGGVNKKYKEKGRSLLFNLKDKNNPELRESVMSG 408


>At2g38560.1 68415.m04737 transcription factor S-II (TFIIS)
           domain-containing protein similar to SP|P49373
           Transcription elongation factor S-II (TFIIS)
           {Schizosaccharomyces pombe}; contains Pfam profile
           PF01096: Transcription factor S-II (TFIIS)
          Length = 378

 Score = 32.7 bits (71), Expect = 0.22
 Identities = 26/94 (27%), Positives = 36/94 (38%), Gaps = 7/94 (7%)
 Frame = +3

Query: 390 PASFPPQSNTTDAVRLKCRELLTQAL-KAAGETSNA------CGSPXXXXXXXXXCIYAE 548
           P          D VR K RELL +AL + AGE  +          P          ++ +
Sbjct: 197 PPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYERESVNASDPLRVAVSVESLMFEK 256

Query: 549 FKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNG 650
              +    K + RS + NL+D  NP LR     G
Sbjct: 257 LGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTG 290


>At5g11430.1 68418.m01335 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 871

 Score = 31.9 bits (69), Expect = 0.38
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = +3

Query: 537 IYAEFKNTDMRYKNRVRSRVANLKDPKNPTLR 632
           +Y  F   + +Y+ R RS + NLKD  NP LR
Sbjct: 270 LYKLFGGVNKKYRERGRSLLFNLKDKNNPELR 301


>At2g25640.1 68415.m03073 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 643

 Score = 31.5 bits (68), Expect = 0.51
 Identities = 18/72 (25%), Positives = 28/72 (38%)
 Frame = +3

Query: 435 LKCRELLTQALKAAGETSNACGSPXXXXXXXXXCIYAEFKNTDMRYKNRVRSRVANLKDP 614
           + C+  +  A+   G        P          ++  F   + +YK + RS + NLKD 
Sbjct: 242 VNCQNEMIGAMANDGSLEKLLLDPQVLAFEIETELFKLFGGVNKKYKEKGRSLLFNLKDK 301

Query: 615 KNPTLRTNFFNG 650
            NP LR     G
Sbjct: 302 SNPKLREKVMYG 313


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,515,089
Number of Sequences: 28952
Number of extensions: 158722
Number of successful extensions: 419
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 418
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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