BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0644 (571 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 31 0.16 SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 29 0.48 SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+] |Schizosacc... 27 1.5 SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 27 1.5 SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schi... 27 1.9 SPAC1751.02c |rsm19||mitochondrial ribosomal protein subunit S19... 25 5.9 SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo... 25 5.9 SPAC227.08c |yth1||zinc finger protein Yth1|Schizosaccharomyces ... 25 5.9 SPBC21.07c |ppk24||serine/threonine protein kinase Ppk24|Schizos... 25 5.9 SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 25 7.8 SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces p... 25 7.8 SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster ... 25 7.8 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 30.7 bits (66), Expect = 0.16 Identities = 16/55 (29%), Positives = 31/55 (56%), Gaps = 2/55 (3%) Frame = +2 Query: 56 IMASKFKSEKIGIVGSGLIGRSWA--MLFASVGYQVTVYDVVAKQITDAIEDIKY 214 + +S FKS KI IVG+G +G + A +L + + ++ + D+ K+ D+ + Sbjct: 13 VRSSSFKSIKIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNH 67 >SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 466 Score = 29.1 bits (62), Expect = 0.48 Identities = 11/36 (30%), Positives = 22/36 (61%) Frame = +2 Query: 68 KFKSEKIGIVGSGLIGRSWAMLFASVGYQVTVYDVV 175 + + + +GI+G G IG ++L ++G V YD++ Sbjct: 192 EIRGKTLGIIGYGHIGSQLSVLAEAMGLHVVYYDIL 227 >SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+] |Schizosaccharomyces pombe|chr 3|||Manual Length = 431 Score = 27.5 bits (58), Expect = 1.5 Identities = 11/29 (37%), Positives = 19/29 (65%) Frame = +2 Query: 83 KIGIVGSGLIGRSWAMLFASVGYQVTVYD 169 ++GI+G G +GR +A + G++V V D Sbjct: 6 QVGIIGFGDMGRLYAEYISKAGWRVNVCD 34 >SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 27.5 bits (58), Expect = 1.5 Identities = 9/30 (30%), Positives = 20/30 (66%) Frame = +2 Query: 80 EKIGIVGSGLIGRSWAMLFASVGYQVTVYD 169 +++GI+G G IG+S+A +G ++ ++ Sbjct: 160 KRVGIIGMGAIGKSFAQKILPLGCEIVYHN 189 >SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schizosaccharomyces pombe|chr 1|||Manual Length = 511 Score = 27.1 bits (57), Expect = 1.9 Identities = 12/43 (27%), Positives = 26/43 (60%) Frame = +2 Query: 41 VASTVIMASKFKSEKIGIVGSGLIGRSWAMLFASVGYQVTVYD 169 V+ST ++ +K+ ++G G+IG +++ +G +VTV + Sbjct: 205 VSSTGALSLSEVPKKMTVLGGGIIGLEMGSVWSRLGAEVTVVE 247 >SPAC1751.02c |rsm19||mitochondrial ribosomal protein subunit S19|Schizosaccharomyces pombe|chr 1|||Manual Length = 108 Score = 25.4 bits (53), Expect = 5.9 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +2 Query: 278 QCIKGSTDLETAVKGAIFVQECV 346 QCIKG+ ++TAV+ A + V Sbjct: 42 QCIKGNVPIKTAVRSATILPRMV 64 >SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 1616 Score = 25.4 bits (53), Expect = 5.9 Identities = 8/28 (28%), Positives = 21/28 (75%) Frame = +2 Query: 332 VQECVPENLDLKKKVFQNLDSVVDDNTI 415 ++ +P + L KK+F+N ++VV+++++ Sbjct: 1294 IRASLPTLICLSKKIFENAENVVENHSL 1321 >SPAC227.08c |yth1||zinc finger protein Yth1|Schizosaccharomyces pombe|chr 1|||Manual Length = 170 Score = 25.4 bits (53), Expect = 5.9 Identities = 9/14 (64%), Positives = 9/14 (64%) Frame = +1 Query: 121 MGDVVCLSWLSGYC 162 MG VVC WL G C Sbjct: 49 MGSVVCKHWLRGLC 62 >SPBC21.07c |ppk24||serine/threonine protein kinase Ppk24|Schizosaccharomyces pombe|chr 2|||Manual Length = 461 Score = 25.4 bits (53), Expect = 5.9 Identities = 19/70 (27%), Positives = 33/70 (47%) Frame = +3 Query: 264 LANNFNV*KGLRIWKPQLKELYLFRNVFQRILTLKKRCSRISTA*LTITRYYQVQQVLLC 443 L + FN G + + + LF+ + +++LTL+ +C + L Y Q Q++ Sbjct: 197 LTSVFNKSAGFCLVQEYCPQGDLFKQIEEKVLTLEDKCCYLKQI-LQAVAYLQSQRIA-- 253 Query: 444 HHYSLKD*NI 473 H LK NI Sbjct: 254 -HRDLKPENI 262 >SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 25.0 bits (52), Expect = 7.8 Identities = 11/40 (27%), Positives = 20/40 (50%), Gaps = 1/40 (2%) Frame = +2 Query: 56 IMASKFKSEKIGIVGSGLIGRSWAMLF-ASVGYQVTVYDV 172 ++ + +G++G+G IG + A F G V YD+ Sbjct: 137 LLGCDIHGKTVGVIGTGKIGSNVAKCFKMGFGCDVLAYDI 176 >SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces pombe|chr 1|||Manual Length = 1272 Score = 25.0 bits (52), Expect = 7.8 Identities = 10/26 (38%), Positives = 15/26 (57%) Frame = +2 Query: 335 QECVPENLDLKKKVFQNLDSVVDDNT 412 + C NLD+ K NLDS++ D + Sbjct: 222 KHCTSWNLDMSKSNSLNLDSIIVDTS 247 >SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 782 Score = 25.0 bits (52), Expect = 7.8 Identities = 19/89 (21%), Positives = 40/89 (44%), Gaps = 2/89 (2%) Frame = +2 Query: 95 VGSGLIGRSWAMLFASVGYQVTVYDVVAKQITDAIEDIK--YQLHTLENDGLLRGELKAS 268 + +G+I ++A+ F + Y +T Y+ + + I E +K + + D L++ + K Sbjct: 561 LNAGIIQAAYALCFPNTDYALTAYNDL-QYICRIFEHLKGGFMFSNKDYDFLIQLKSKVF 619 Query: 269 EQFQCIKGSTDLETAVKGAIFVQECVPEN 355 +FQ E + F+ VP + Sbjct: 620 NRFQLSSQGLTPEDLTEDVPFLHFNVPSS 648 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,468,774 Number of Sequences: 5004 Number of extensions: 51346 Number of successful extensions: 142 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 242064240 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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