BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0628 (627 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 109 3e-25 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 102 5e-23 SPBC15D4.02 |||transcription factor, zf-fungal binuclear cluster... 31 0.10 SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 29 0.42 SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyce... 26 3.9 SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 26 5.1 SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces pomb... 25 6.8 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 109 bits (262), Expect = 3e-25 Identities = 58/141 (41%), Positives = 87/141 (61%), Gaps = 18/141 (12%) Frame = +2 Query: 2 AMSEYDQVLHEDETTNRMQESLKLFDSICNNKWFTDTSIILFLNKKDLFEEKIRRSPLTI 181 +++ YDQ L ED + N+MQE+L L+DSICN+ WF+++++ILFLNK DLF+ K+ SP+ Sbjct: 266 SLAGYDQCLVEDNSGNQMQEALLLWDSICNSSWFSESAMILFLNKLDLFKRKVHISPIQK 325 Query: 182 CFPEYTGAQEYGEAAAYIQAQ------------------FEAKNKSTTKEIYCHMTCATD 307 FP+Y QE G ++Q Q FE+ N+ ++ YCH T ATD Sbjct: 326 HFPDY---QEVGSTPTFVQTQCPLADNAVRSGMYYFYLKFESLNRIASRSCYCHFTTATD 382 Query: 308 TNNIQFVFDAVTDVIIANNLR 370 T+ +Q V +V D I++NNL+ Sbjct: 383 TSLLQRVMVSVQDTIMSNNLQ 403 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 102 bits (244), Expect = 5e-23 Identities = 53/131 (40%), Positives = 79/131 (60%), Gaps = 3/131 (2%) Frame = +2 Query: 2 AMSEYDQVLHEDETT--NRMQESLKLFDSICNNKWFTDTSIILFLNKKDLFEEKIRRSPL 175 ++++YD+ L+E NR+ ES+ LFDSI N++WF +SIILFLNK DLF +K+ P Sbjct: 224 SLNDYDKKLYERAAPERNRLVESISLFDSIINSQWFMHSSIILFLNKFDLFRKKLEHVPF 283 Query: 176 TICFPEYTGAQEYGEAAAYIQAQFEAKNKSTTK-EIYCHMTCATDTNNIQFVFDAVTDVI 352 FP+Y G YI F + + K IY H+T A DT+NI+ VF AV + I Sbjct: 284 QDYFPQYEGKNSVKSITRYILWLFVNPSINRAKHNIYPHITTAVDTSNIKVVFSAVKETI 343 Query: 353 IANNLRGCGLY 385 + ++L+ G++ Sbjct: 344 LQHSLKEAGMF 354 >SPBC15D4.02 |||transcription factor, zf-fungal binuclear cluster type|Schizosaccharomyces pombe|chr 2|||Manual Length = 419 Score = 31.5 bits (68), Expect = 0.10 Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 2/40 (5%) Frame = -2 Query: 329 TQTGCCSCR-WRTSCGSRSPSWWTC-SSLRTAPGCTRPPR 216 T+TGC +CR R C R P + C S R G T PR Sbjct: 42 TKTGCLTCRKRRIKCDERKPICYNCIKSKRQCEGYTHFPR 81 >SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 394 Score = 29.5 bits (63), Expect = 0.42 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = -3 Query: 622 VFRI*FEMTSCSLLESCNKLDTIVSGL 542 V I + CSLL CN+LD + SGL Sbjct: 136 VLHILMKENKCSLLYPCNRLDRLTSGL 162 >SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 26.2 bits (55), Expect = 3.9 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = +1 Query: 292 DVRHRHEQHPVCVRRGDRRDH 354 D +RH+Q +CV DRR H Sbjct: 513 DALNRHKQRNICVGGVDRRQH 533 >SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 25.8 bits (54), Expect = 5.1 Identities = 9/23 (39%), Positives = 12/23 (52%) Frame = +1 Query: 250 SEEQVHHEGDLLPHDVRHRHEQH 318 S++ +HHEG H H H H Sbjct: 290 SDKDLHHEGHHHHHHHHHHHHSH 312 >SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 25.4 bits (53), Expect = 6.8 Identities = 16/55 (29%), Positives = 26/55 (47%) Frame = -1 Query: 381 RPQPRRLLAMITSVTASNTNWMLFVSVAHVMWQ*ISFVVDLFFASNCAWMYAAAS 217 R QP+R A+ VTA N W F V+ + S + + F++ + +A S Sbjct: 7 RLQPQRFAALPNPVTAENKFWRSF--KVPVVAKEYSAITSIHFSAESPYDFAVTS 59 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,371,278 Number of Sequences: 5004 Number of extensions: 47016 Number of successful extensions: 143 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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