BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0619 (412 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 34 0.010 SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc... 26 2.6 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 26 2.6 SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 25 4.6 SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 25 6.1 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 25 6.1 >SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe|chr 1|||Manual Length = 727 Score = 33.9 bits (74), Expect = 0.010 Identities = 13/49 (26%), Positives = 28/49 (57%) Frame = +3 Query: 264 DGDGVSTSIHNTPERPTKSERRSSATPPLDSETPEKILDRRLSKDKKSV 410 DG+G+ST + + ++PT+ ++ PP + ++D +S D ++V Sbjct: 411 DGEGISTMANKSNDQPTRRASTHNSGPPAKRKRESLVIDLTISDDDENV 459 >SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 745 Score = 25.8 bits (54), Expect = 2.6 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +3 Query: 120 YESSSGESDDENQNDISDQQLQE 188 YE+S+ ES+DE +D S ++ E Sbjct: 686 YETSTSESEDEETDDTSQEEDNE 708 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 25.8 bits (54), Expect = 2.6 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +3 Query: 285 SIHNTPERPTKSERRSSATPPLDSETPEKILDRRLSK 395 S+ N ER + + ++A SET + L+RRLSK Sbjct: 628 SLANIDERIMRQNQAATANTNTVSETDIEALERRLSK 664 >SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1019 Score = 25.0 bits (52), Expect = 4.6 Identities = 9/25 (36%), Positives = 20/25 (80%) Frame = +3 Query: 138 ESDDENQNDISDQQLQEMIRRKKIE 212 +SDDE+ +++ ++L+E+ R+K +E Sbjct: 796 DSDDEDYSNLRREKLKELRRQKLLE 820 >SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1055 Score = 24.6 bits (51), Expect = 6.1 Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%) Frame = +3 Query: 120 YESSSGESDDENQNDISD-QQLQEMIRRKKIE 212 YE SS + + E ND+ + ++ +E +RRK +E Sbjct: 167 YEKSS-DPETERLNDLREREEFEERLRRKDLE 197 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 24.6 bits (51), Expect = 6.1 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -1 Query: 115 PNPRPEAMAASAFDLPAFMALMRDAF 38 P PR + AA FD+P + L +D F Sbjct: 475 PKPRQQFDAADKFDVPFAVILGQDEF 500 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,401,735 Number of Sequences: 5004 Number of extensions: 22887 Number of successful extensions: 84 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 83 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 84 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 142254980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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