BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0616 (687 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_8108| Best HMM Match : GST_N (HMM E-Value=3.9e-14) 111 4e-25 SB_3922| Best HMM Match : No HMM Matches (HMM E-Value=.) 89 4e-18 SB_48592| Best HMM Match : Galactosyl_T (HMM E-Value=1.5e-26) 49 3e-06 SB_25847| Best HMM Match : Methuselah_N (HMM E-Value=0.48) 32 0.50 SB_9381| Best HMM Match : TAF4 (HMM E-Value=2.9) 32 0.50 SB_26062| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.1 SB_41537| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.1 >SB_8108| Best HMM Match : GST_N (HMM E-Value=3.9e-14) Length = 238 Score = 111 bits (268), Expect = 4e-25 Identities = 58/156 (37%), Positives = 89/156 (57%), Gaps = 4/156 (2%) Frame = +1 Query: 184 MSEKHLQTGDVLPPYSGKLRVFAMRFCPYAERTVLTLNAKNIPYDLVFINLDQKPEW-IF 360 M + HL GD PP KLR+++MRFCPYAER L L AK + Y+ + INL KP+W + Sbjct: 1 MPQSHLSNGDPRPPPGDKLRLYSMRFCPYAERPRLVLAAKGVDYECININLKNKPDWYLA 60 Query: 361 NFSPKGTVPAL*YEPGKALFDSNIINVYLDEKYPEIPLQASDPLRRAQDKILVESFAPAQ 540 +P+G VP + G+ L +S + YLDE +P+ P+ SD + + ++L++ F Sbjct: 61 EPNPRGLVPMIEMPDGRLLPESLLCCEYLDELFPQNPMYPSDAFEKNRQRLLIDRFGKVT 120 Query: 541 SAYYTAA---FNAQALEPSMVETYHKDLRDYKKSLK 639 S++Y + AL+ ET +K+L Y+ LK Sbjct: 121 SSFYQMLMRDMDEDALK-GQTETLNKELSLYENELK 155 >SB_3922| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 236 Score = 88.6 bits (210), Expect = 4e-18 Identities = 49/121 (40%), Positives = 69/121 (57%), Gaps = 3/121 (2%) Frame = +1 Query: 175 SFNMSEKHLQTGDVLPPY-SGKLRVFAMRFCPYAERTVLTLNAKNIPYDLVFINLDQKPE 351 S M H+ G P KLR+++MRFCP+AER L L AK + Y+ V +NL KPE Sbjct: 8 SITMPVTHISKGSSRPAKPQNKLRLYSMRFCPFAERPRLVLAAKGLDYECVNVNLKSKPE 67 Query: 352 WIFNFSP--KGTVPAL*YEPGKALFDSNIINVYLDEKYPEIPLQASDPLRRAQDKILVES 525 W F P +G VP L GK + +S II +L++ Y +IPL DP +++ K+L + Sbjct: 68 W-FQTHPDCEGKVPTLETMDGKLIPESVIICEFLEDYYRKIPLYPCDPYAKSRQKLLAQR 126 Query: 526 F 528 F Sbjct: 127 F 127 >SB_48592| Best HMM Match : Galactosyl_T (HMM E-Value=1.5e-26) Length = 492 Score = 49.2 bits (112), Expect = 3e-06 Identities = 25/87 (28%), Positives = 50/87 (57%), Gaps = 2/87 (2%) Frame = +1 Query: 259 FCPYAERTVLTLNAKNIPYDLVFIN-LDQKPEWIFNFSPKGTVPAL*YEPGKALFDSNII 435 FCP+A+R + + AK ++ + N ++ PEW+ +P G VP + + G A+++S+I Sbjct: 269 FCPFAQRAWIAMLAKKAEFEYIEQNPYNKTPEWL-AINPNGLVPVIVHN-GNAVYESSIC 326 Query: 436 NVYLDEKY-PEIPLQASDPLRRAQDKI 513 ++DE + + + DP +RA ++ Sbjct: 327 IEFIDEAFSTPVTILPKDPYKRAHGRM 353 >SB_25847| Best HMM Match : Methuselah_N (HMM E-Value=0.48) Length = 390 Score = 31.9 bits (69), Expect = 0.50 Identities = 25/80 (31%), Positives = 36/80 (45%), Gaps = 1/80 (1%) Frame = +2 Query: 356 SSTLARRVLCQLCNMNLVRHSLTATL*MFILMKSIQRFLCKHQTLCAELKTK-FSLRALL 532 S L R L CN+NLVRH L +++Q LC +KT+ LR LL Sbjct: 221 SRNLVPRGLLMRCNLNLVRHD--ERLRQLCRQQTLQSSSKILYELCNSVKTEVLRLRGLL 278 Query: 533 QHNQLTIQQHSMHKLSSQAW 592 Q + T Q + ++ + W Sbjct: 279 QDARKTAFQSTDYQRALSIW 298 >SB_9381| Best HMM Match : TAF4 (HMM E-Value=2.9) Length = 210 Score = 31.9 bits (69), Expect = 0.50 Identities = 25/80 (31%), Positives = 36/80 (45%), Gaps = 1/80 (1%) Frame = +2 Query: 356 SSTLARRVLCQLCNMNLVRHSLTATL*MFILMKSIQRFLCKHQTLCAELKTK-FSLRALL 532 S L R L CN+NLVRH L +++Q LC +KT+ LR LL Sbjct: 41 SRNLVPRGLLMRCNLNLVRHD--ERLRQLCRQQTLQSSSKILYELCNSVKTEVLRLRGLL 98 Query: 533 QHNQLTIQQHSMHKLSSQAW 592 Q + T Q + ++ + W Sbjct: 99 QDARKTAFQSTDYQRALSIW 118 >SB_26062| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1535 Score = 27.9 bits (59), Expect = 8.1 Identities = 17/59 (28%), Positives = 29/59 (49%), Gaps = 2/59 (3%) Frame = +1 Query: 472 LQASDPLRRAQDKILVESFAPAQSAYYTAAFNAQ--ALEPSMVETYHKDLRDYKKSLKR 642 +Q D L + Q+K+ E+ A+ Y + LE ++ KDL+ KKSL++ Sbjct: 894 IQMRDDLFKLQEKLKSENVQAARQGYREILGTKRDLKLEKRLLREQKKDLKKLKKSLRK 952 >SB_41537| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 71 Score = 27.9 bits (59), Expect = 8.1 Identities = 15/39 (38%), Positives = 20/39 (51%) Frame = +2 Query: 476 KHQTLCAELKTKFSLRALLQHNQLTIQQHSMHKLSSQAW 592 KH L +LKT F L +L H Q TI S+ ++W Sbjct: 18 KHGILHEDLKTGFMLYDVLAHGQNTILPSSLWIHERESW 56 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,253,250 Number of Sequences: 59808 Number of extensions: 357291 Number of successful extensions: 781 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 750 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 779 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1781448916 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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