BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0607 (522 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 77 1e-15 SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 71 8e-14 SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 68 1e-12 SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 42 7e-05 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 30 0.18 SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|... 26 3.0 SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosa... 25 6.8 SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 6.8 >SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 215 Score = 77.4 bits (182), Expect = 1e-15 Identities = 41/107 (38%), Positives = 63/107 (58%), Gaps = 1/107 (0%) Frame = +2 Query: 29 SWFSGSQLRVHLDIQVGEHAVDYANVAQKE-KLTELQLRIRQLLDQVHQITKEQAYQRQR 205 +WFS ++ R+H+D+ + Y +A E + +L R+ L ++ I EQ QR R Sbjct: 112 AWFSSTKTRLHVDLASDDK---YNLIASSEDEAKDLTDRVAALNTRLESILGEQMLQRSR 168 Query: 206 EERFRQTSESTNQRVLWWSLLQTGVLVIIGYWQMRHLKSFFEAKKLV 346 E FR TSES N RV+ W+++Q VL++ WQ+ HL+ FF +KLV Sbjct: 169 EMTFRDTSESANSRVVRWTIVQIVVLLVTCIWQLSHLQRFFVKEKLV 215 >SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schizosaccharomyces pombe|chr 3|||Manual Length = 199 Score = 71.3 bits (167), Expect = 8e-14 Identities = 36/88 (40%), Positives = 52/88 (59%) Frame = +2 Query: 83 HAVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWS 262 H V Y + + L ++RQL D + ++ EQ Y RE R T+ESTN RV WWS Sbjct: 112 HGVIYISDEDLDANNPLLGKVRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWS 171 Query: 263 LLQTGVLVIIGYWQMRHLKSFFEAKKLV 346 +LQT +LV + +Q+ +LK FE K++V Sbjct: 172 ILQTVILVSVCVFQIFYLKRLFEVKRVV 199 >SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 67.7 bits (158), Expect = 1e-12 Identities = 35/97 (36%), Positives = 58/97 (59%) Frame = +2 Query: 56 VHLDIQVGEHAVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSES 235 V L+ VG A DY+++ + L ++ IR+ D + +I + Y + RE RFR T+ES Sbjct: 119 VKLEFTVGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNES 178 Query: 236 TNQRVLWWSLLQTGVLVIIGYWQMRHLKSFFEAKKLV 346 TN+RV ++ L L ++ WQ+ +L+SFF+ K L+ Sbjct: 179 TNERVKNFAYLTFISLFVLVIWQILYLRSFFQRKHLI 215 >SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 210 Score = 41.5 bits (93), Expect = 7e-05 Identities = 21/75 (28%), Positives = 42/75 (56%) Frame = +2 Query: 107 AQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLV 286 A+ K +Q + ++ + +I + Q Y + RE R T +ST R+ W+SL ++ ++V Sbjct: 129 AKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESIMVV 188 Query: 287 IIGYWQMRHLKSFFE 331 + Q+ +K+FF+ Sbjct: 189 ALSALQVFIVKTFFK 203 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 30.3 bits (65), Expect = 0.18 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = +3 Query: 156 WIKYTRSQRSRLTRGKEKSVFDKHQKAQTSECSGGVCYRQ 275 W+ + S+R L + ++++V K ++ Q CS VC R+ Sbjct: 266 WLSLSESERRSLVKVEKEAVLQKMKEQQKYSCSCSVCGRK 305 >SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 785 Score = 26.2 bits (55), Expect = 3.0 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = +1 Query: 229 RKHKPASALVEFATDRCPCHHRLLADETSQEL 324 RKH+ + +V DR C H L E +++L Sbjct: 716 RKHQADACIVRVMKDRKVCEHNQLMAEVTRQL 747 >SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 25.0 bits (52), Expect = 6.8 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Frame = +2 Query: 83 HAVDYANVAQKEKLTELQLRIRQL--LDQVHQITKEQAYQ 196 +AVDY + K+K+ +L+ I +L D V + E Y+ Sbjct: 147 NAVDYIIQSAKDKVQKLEAEIEELSTADDVDDVLLESKYE 186 >SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 25.0 bits (52), Expect = 6.8 Identities = 10/35 (28%), Positives = 20/35 (57%) Frame = +2 Query: 161 QVHQITKEQAYQRQREERFRQTSESTNQRVLWWSL 265 ++ + K++ Q REER+RQ +E + ++ L Sbjct: 535 EISKRRKQRKAQEMREERYRQRAERDTEEQIYSEL 569 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,786,093 Number of Sequences: 5004 Number of extensions: 29454 Number of successful extensions: 111 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 107 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 110 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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