BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0560 (707 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 55 1e-08 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 41 2e-04 SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 35 0.010 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 35 0.013 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 34 0.017 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.21 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 30 0.37 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.86 SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|... 26 6.1 SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M... 26 6.1 SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 25 8.0 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 8.0 SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 25 8.0 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 54.8 bits (126), Expect = 1e-08 Identities = 41/128 (32%), Positives = 65/128 (50%), Gaps = 8/128 (6%) Frame = +2 Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 523 + ++ F D+DQ G ITS EL ++ + GQ+ + MI D D NG I+ EF Sbjct: 13 EFREAFSLFDRDQDGNITSNEL-GVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFL 71 Query: 524 ----DKLYTYVN--QWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSN 685 K+ N + FK +D D +G+I EL+ LT +G R S E + + + ++ Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREAD 131 Query: 686 TN-DGKIS 706 T+ DG I+ Sbjct: 132 TDGDGVIN 139 Score = 40.3 bits (90), Expect = 3e-04 Identities = 22/62 (35%), Positives = 35/62 (56%) Frame = +2 Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 526 +V++ F+ DKD +G+IT EL L + G+ S+ MI D D +G IN +EF Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSL-GERLSQEEVADMIREADTDGDGVINYEEFS 144 Query: 527 KL 532 ++ Sbjct: 145 RV 146 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 41.1 bits (92), Expect = 2e-04 Identities = 33/120 (27%), Positives = 54/120 (45%), Gaps = 3/120 (2%) Frame = +2 Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 526 ++++ F +DKD G I ++++ L + Q SE + N M + N L Sbjct: 49 ELKEAFALLDKDGDGNIGREDVKTMLTSLN-QDASEDSINHMFESINPPINLAAFLTAMG 107 Query: 527 KLYTYV---NQWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDG 697 + + N L F T+D QSG I + AL+ MG R P+ + + RS T+ G Sbjct: 108 SMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESIL-RSYTSHG 166 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 35.1 bits (77), Expect = 0.010 Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 9/98 (9%) Frame = +2 Query: 347 QVQQWFRAVDKD-QSGFITSTELRSALVNAQGQTFSETAC--NLMIGMFDKDRNGQINLD 517 ++QQW++ KD SG + +E + + Q F + + + +FD D+NG I+ Sbjct: 26 ELQQWYKGFFKDCPSGHLNKSEFQK--IYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFK 83 Query: 518 EFDKLYTYV------NQWLAVFKTYDTDQSGFIDEGEL 613 EF + ++ + F+ YD D +G I E+ Sbjct: 84 EFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEM 121 Score = 27.9 bits (59), Expect = 1.5 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 7/58 (12%) Frame = +2 Query: 476 GMFDKDRNGQINLDEFDKLYTYVNQW-------LAVFKTYDTDQSGFIDEGELSKALT 628 G F +G +N EF K+Y + VF +D D++G+ID E AL+ Sbjct: 33 GFFKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALS 90 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +2 Query: 452 ETACNLMIGMFDKDRNGQINLDEF 523 E N + M DK+++GQ+ L+EF Sbjct: 146 EKRVNKIFNMMDKNKDGQLTLEEF 169 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 34.7 bits (76), Expect = 0.013 Identities = 17/48 (35%), Positives = 26/48 (54%) Frame = +2 Query: 563 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKIS 706 F+ +D D +G I GEL LT +G + S E ++ L K DG ++ Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVN 130 Score = 28.3 bits (60), Expect = 1.1 Identities = 18/54 (33%), Positives = 27/54 (50%) Frame = +2 Query: 362 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 523 F+ DKD +G I ELR L + + +E L+ G+ KD G +N +F Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD--GMVNYHDF 134 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 34.3 bits (75), Expect = 0.017 Identities = 28/103 (27%), Positives = 45/103 (43%), Gaps = 7/103 (6%) Frame = +2 Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 523 ++++ F +D +QSG I E S A S + + + D+D G ++ EF Sbjct: 25 RIRKRFIKIDANQSGSIDRNEFLSIPSVA-----SNPLASRLFSVVDEDGGGDVDFQEFI 79 Query: 524 DKLYTYV------NQWLAVFKTYDTDQSGFIDEGELSKALTQM 634 + L + + FK YD D+ G+I GEL L M Sbjct: 80 NSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMM 122 Score = 31.5 bits (68), Expect = 0.12 Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 4/66 (6%) Frame = +2 Query: 362 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGM----FDKDRNGQINLDEFDK 529 F+ D D+ G+I++ EL L G E ++ DKDR+G+I+ +EF Sbjct: 99 FKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKD 158 Query: 530 LYTYVN 547 + + N Sbjct: 159 IVSGSN 164 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 30.7 bits (66), Expect = 0.21 Identities = 14/48 (29%), Positives = 26/48 (54%) Frame = +2 Query: 563 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKIS 706 F +D G+ID +L ++ Q+G + E + ++ + TN GK+S Sbjct: 18 FDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTN-GKVS 64 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 29.9 bits (64), Expect = 0.37 Identities = 15/54 (27%), Positives = 26/54 (48%) Frame = +2 Query: 362 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 523 FR DKD SG+I + + + + G+ S+ LM+ D +G + +F Sbjct: 84 FRVFDKDNSGYIETAKF-ADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 28.7 bits (61), Expect = 0.86 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%) Frame = +2 Query: 563 FKTYDTDQSGFIDEGELSKALTQMGFRF-SPEFINILTKRSNTNDG 697 FK +D+D+ ID EL A+ +GF E + IL T G Sbjct: 43 FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKG 88 >SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 539 Score = 25.8 bits (54), Expect = 6.1 Identities = 20/66 (30%), Positives = 29/66 (43%) Frame = +2 Query: 254 GQAAYGGMPPGQLEIGHGPYPSIGVGGTITPQVQQWFRAVDKDQSGFITSTELRSALVNA 433 G+ A+GG L+ GP + V G I + W + SG + +TEL S V + Sbjct: 304 GRQAWGGFSREALK-NWGPLCRLAVPGVIMICSEYWAFELVTFASGVLGTTELASMSVLS 362 Query: 434 QGQTFS 451 T S Sbjct: 363 TTSTLS 368 >SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 1115 Score = 25.8 bits (54), Expect = 6.1 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = -2 Query: 493 VFVEHTNHQIASSFGKSLT 437 V+ H N QI SSFG SLT Sbjct: 33 VWTGHKNGQIKSSFGPSLT 51 >SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 472 Score = 25.4 bits (53), Expect = 8.0 Identities = 9/17 (52%), Positives = 11/17 (64%) Frame = +2 Query: 224 LQMAYNSGYPGQAAYGG 274 +Q Y+ GYPG YGG Sbjct: 61 MQNKYSEGYPGARYYGG 77 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 25.4 bits (53), Expect = 8.0 Identities = 8/22 (36%), Positives = 16/22 (72%) Frame = -1 Query: 494 GLCRTYQSSDCKQFRKKFDLER 429 GLC T ++D + F+++ ++ER Sbjct: 2002 GLCNTLLTTDSETFKRRLNIER 2023 >SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit Tim50 |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.4 bits (53), Expect = 8.0 Identities = 10/34 (29%), Positives = 19/34 (55%) Frame = -1 Query: 320 WKGTVHVQFLIGLVAFHRKLPVLDIRNCRPFVVS 219 W G + L+GL+ + ++DI++ RP + S Sbjct: 297 WTGNPKDKELVGLIPLLEFIAIMDIKDVRPVLKS 330 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,991,785 Number of Sequences: 5004 Number of extensions: 62600 Number of successful extensions: 164 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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