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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0560
         (707 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    55   1e-08
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    41   2e-04
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    35   0.010
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    35   0.013
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    34   0.017
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    31   0.21 
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    30   0.37 
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    29   0.86 
SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|...    26   6.1  
SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M...    26   6.1  
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo...    25   8.0  
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc...    25   8.0  
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni...    25   8.0  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 54.8 bits (126), Expect = 1e-08
 Identities = 41/128 (32%), Positives = 65/128 (50%), Gaps = 8/128 (6%)
 Frame = +2

Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 523
           + ++ F   D+DQ G ITS EL   ++ + GQ+ +      MI   D D NG I+  EF 
Sbjct: 13  EFREAFSLFDRDQDGNITSNEL-GVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFL 71

Query: 524 ----DKLYTYVN--QWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSN 685
                K+    N  +    FK +D D +G+I   EL+  LT +G R S E +  + + ++
Sbjct: 72  TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREAD 131

Query: 686 TN-DGKIS 706
           T+ DG I+
Sbjct: 132 TDGDGVIN 139



 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 22/62 (35%), Positives = 35/62 (56%)
 Frame = +2

Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 526
           +V++ F+  DKD +G+IT  EL   L +  G+  S+     MI   D D +G IN +EF 
Sbjct: 86  EVREAFKVFDKDGNGYITVEELTHVLTSL-GERLSQEEVADMIREADTDGDGVINYEEFS 144

Query: 527 KL 532
           ++
Sbjct: 145 RV 146


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 33/120 (27%), Positives = 54/120 (45%), Gaps = 3/120 (2%)
 Frame = +2

Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 526
           ++++ F  +DKD  G I   ++++ L +   Q  SE + N M    +   N    L    
Sbjct: 49  ELKEAFALLDKDGDGNIGREDVKTMLTSLN-QDASEDSINHMFESINPPINLAAFLTAMG 107

Query: 527 KLYTYV---NQWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDG 697
            +   +   N  L  F T+D  QSG I    +  AL+ MG R  P+ +  +  RS T+ G
Sbjct: 108 SMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESIL-RSYTSHG 166


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 35.1 bits (77), Expect = 0.010
 Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 9/98 (9%)
 Frame = +2

Query: 347 QVQQWFRAVDKD-QSGFITSTELRSALVNAQGQTFSETAC--NLMIGMFDKDRNGQINLD 517
           ++QQW++   KD  SG +  +E +   +  Q   F + +     +  +FD D+NG I+  
Sbjct: 26  ELQQWYKGFFKDCPSGHLNKSEFQK--IYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFK 83

Query: 518 EFDKLYTYV------NQWLAVFKTYDTDQSGFIDEGEL 613
           EF    +        ++ +  F+ YD D +G I   E+
Sbjct: 84  EFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEM 121



 Score = 27.9 bits (59), Expect = 1.5
 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 7/58 (12%)
 Frame = +2

Query: 476 GMFDKDRNGQINLDEFDKLYTYVNQW-------LAVFKTYDTDQSGFIDEGELSKALT 628
           G F    +G +N  EF K+Y     +         VF  +D D++G+ID  E   AL+
Sbjct: 33  GFFKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALS 90



 Score = 27.1 bits (57), Expect = 2.6
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +2

Query: 452 ETACNLMIGMFDKDRNGQINLDEF 523
           E   N +  M DK+++GQ+ L+EF
Sbjct: 146 EKRVNKIFNMMDKNKDGQLTLEEF 169


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 34.7 bits (76), Expect = 0.013
 Identities = 17/48 (35%), Positives = 26/48 (54%)
 Frame = +2

Query: 563 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKIS 706
           F+ +D D +G I  GEL   LT +G + S E ++ L K     DG ++
Sbjct: 83  FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVN 130



 Score = 28.3 bits (60), Expect = 1.1
 Identities = 18/54 (33%), Positives = 27/54 (50%)
 Frame = +2

Query: 362 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 523
           F+  DKD +G I   ELR  L +   +  +E    L+ G+  KD  G +N  +F
Sbjct: 83  FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD--GMVNYHDF 134


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 34.3 bits (75), Expect = 0.017
 Identities = 28/103 (27%), Positives = 45/103 (43%), Gaps = 7/103 (6%)
 Frame = +2

Query: 347 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 523
           ++++ F  +D +QSG I   E  S    A     S    + +  + D+D  G ++  EF 
Sbjct: 25  RIRKRFIKIDANQSGSIDRNEFLSIPSVA-----SNPLASRLFSVVDEDGGGDVDFQEFI 79

Query: 524 DKLYTYV------NQWLAVFKTYDTDQSGFIDEGELSKALTQM 634
           + L  +        +    FK YD D+ G+I  GEL   L  M
Sbjct: 80  NSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMM 122



 Score = 31.5 bits (68), Expect = 0.12
 Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 4/66 (6%)
 Frame = +2

Query: 362 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGM----FDKDRNGQINLDEFDK 529
           F+  D D+ G+I++ EL   L    G    E     ++       DKDR+G+I+ +EF  
Sbjct: 99  FKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKD 158

Query: 530 LYTYVN 547
           + +  N
Sbjct: 159 IVSGSN 164


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 30.7 bits (66), Expect = 0.21
 Identities = 14/48 (29%), Positives = 26/48 (54%)
 Frame = +2

Query: 563 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKIS 706
           F  +D    G+ID  +L ++  Q+G   + E + ++   + TN GK+S
Sbjct: 18  FDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTN-GKVS 64


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 29.9 bits (64), Expect = 0.37
 Identities = 15/54 (27%), Positives = 26/54 (48%)
 Frame = +2

Query: 362 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 523
           FR  DKD SG+I + +  +  +   G+  S+    LM+   D   +G  +  +F
Sbjct: 84  FRVFDKDNSGYIETAKF-ADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 28.7 bits (61), Expect = 0.86
 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
 Frame = +2

Query: 563 FKTYDTDQSGFIDEGELSKALTQMGFRF-SPEFINILTKRSNTNDG 697
           FK +D+D+   ID  EL  A+  +GF     E + IL     T  G
Sbjct: 43  FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKG 88


>SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 539

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 20/66 (30%), Positives = 29/66 (43%)
 Frame = +2

Query: 254 GQAAYGGMPPGQLEIGHGPYPSIGVGGTITPQVQQWFRAVDKDQSGFITSTELRSALVNA 433
           G+ A+GG     L+   GP   + V G I    + W   +    SG + +TEL S  V +
Sbjct: 304 GRQAWGGFSREALK-NWGPLCRLAVPGVIMICSEYWAFELVTFASGVLGTTELASMSVLS 362

Query: 434 QGQTFS 451
              T S
Sbjct: 363 TTSTLS 368


>SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1115

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 12/19 (63%), Positives = 13/19 (68%)
 Frame = -2

Query: 493 VFVEHTNHQIASSFGKSLT 437
           V+  H N QI SSFG SLT
Sbjct: 33  VWTGHKNGQIKSSFGPSLT 51


>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 472

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 9/17 (52%), Positives = 11/17 (64%)
 Frame = +2

Query: 224 LQMAYNSGYPGQAAYGG 274
           +Q  Y+ GYPG   YGG
Sbjct: 61  MQNKYSEGYPGARYYGG 77


>SPBC30D10.10c |tor1||phosphatidylinositol kinase
            Tor1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2335

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 8/22 (36%), Positives = 16/22 (72%)
 Frame = -1

Query: 494  GLCRTYQSSDCKQFRKKFDLER 429
            GLC T  ++D + F+++ ++ER
Sbjct: 2002 GLCNTLLTTDSETFKRRLNIER 2023


>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
           Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 452

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 10/34 (29%), Positives = 19/34 (55%)
 Frame = -1

Query: 320 WKGTVHVQFLIGLVAFHRKLPVLDIRNCRPFVVS 219
           W G    + L+GL+     + ++DI++ RP + S
Sbjct: 297 WTGNPKDKELVGLIPLLEFIAIMDIKDVRPVLKS 330


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,991,785
Number of Sequences: 5004
Number of extensions: 62600
Number of successful extensions: 164
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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