BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0554 (701 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 1.1 SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 2.0 SPBC713.05 |||WD repeat protein, human MAPK organizer 1 |Schizos... 26 4.5 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 4.5 SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccha... 26 4.5 SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 26 6.0 SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 26 6.0 SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |S... 26 6.0 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 26 6.0 SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 7.9 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 7.9 SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 7.9 SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 7.9 >SPAC1006.07 |||translation initiation factor eIF4A|Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 28.3 bits (60), Expect = 1.1 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +2 Query: 194 AIDLLTNDECRLLLEVEDFFN 256 +I+ +TND+ R++ E+E F+N Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 27.5 bits (58), Expect = 2.0 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +3 Query: 327 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 416 STR + N+VP N L I+C + NK Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103 >SPBC713.05 |||WD repeat protein, human MAPK organizer 1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 297 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +1 Query: 589 LWCCRVNAFNTAAESAGTRDSVAS 660 LW CR N+F+ A +DSV+S Sbjct: 124 LWDCRSNSFSPIQVLADAKDSVSS 147 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.2 bits (55), Expect = 4.5 Identities = 16/62 (25%), Positives = 25/62 (40%) Frame = +2 Query: 458 PSIFENISEVPERVDPQPPAAVLASSPFVTSQPTEELLREFETVYGAVELTHLTPPQSPP 637 P+ S V P P A++ AS+P + S P ++ + T + P Q P Sbjct: 895 PAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHP--PTASGTRVPPVQQPS 952 Query: 638 GP 643 P Sbjct: 953 HP 954 >SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 26.2 bits (55), Expect = 4.5 Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 2/75 (2%) Frame = +3 Query: 300 RSKSITPRVSTRH--HRRS*NQVPPNSLTIFCSS*TVNANKRIFSPTG*RKKLTSLRSSK 473 R + PRV+ + + + N + N+ ++ T+N ++R+ +P+G R L S Sbjct: 529 RGSAFNPRVTRFNVGNEQFSNNIDNNNYNQPYANATMNNSRRLRTPSGERSMRADLSQSP 588 Query: 474 TFLKFLNAWTHNPRQ 518 LN H RQ Sbjct: 589 ASYDSLNVPKHRRRQ 603 >SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 610 Score = 25.8 bits (54), Expect = 6.0 Identities = 13/52 (25%), Positives = 27/52 (51%), Gaps = 1/52 (1%) Frame = +2 Query: 437 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT-EELLREFETV 589 +E + D+ S N S +P+ P V+ SSP + P+ +++ + +T+ Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPSHSDVMGQKDTI 521 >SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 25.8 bits (54), Expect = 6.0 Identities = 13/47 (27%), Positives = 22/47 (46%) Frame = +1 Query: 64 QDKLVHLHKTPTSLDINPSGLLFAFVELNHYNNECESEGVMGCGHRF 204 +D H K I+P LLF F E NH +++ ++ G ++ Sbjct: 345 EDSCFHKIKAAQWHQISPRSLLFQFQEQNHIHHKKIRRKLLAAGWKW 391 >SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 25.8 bits (54), Expect = 6.0 Identities = 9/30 (30%), Positives = 22/30 (73%) Frame = +1 Query: 601 RVNAFNTAAESAGTRDSVASELRSTSTMHS 690 R+N+FN++ ++G+ +VA+E+ + ++ S Sbjct: 207 RLNSFNSSTSTSGSSSNVAAEVSNDASQPS 236 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 25.8 bits (54), Expect = 6.0 Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%) Frame = -1 Query: 611 ALTRQHHKPFRIPAAVLQWAARSQKDLK-LEPLPGVVGPRVQELQK 477 A T H P + P+ +QW +S +DL+ ++ V+ +E K Sbjct: 746 AFTHLGHHPDKTPSEYIQWRFQSGEDLEAMDKASRVISEADEEAMK 791 >SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 731 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = +2 Query: 395 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 511 +D CKQ+ +FS E++ D L SI +S + ++ P Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +2 Query: 212 NDECRLLLEVEDFFNDDC 265 NDECR L + FF+ +C Sbjct: 535 NDECRRLKQCNHFFHREC 552 >SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 95 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = +1 Query: 67 DKLVHLHKTPTSLDINPSGLLFAFVELNH 153 D + H K +S NPS LLF L H Sbjct: 59 DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87 >SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual Length = 304 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%) Frame = +1 Query: 43 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 177 L + ++KLV+L T T LD + ++ A V+ N Y N C+++ Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,672,478 Number of Sequences: 5004 Number of extensions: 51392 Number of successful extensions: 148 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 325165428 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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