BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0548 (668 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 31 0.11 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 30 0.26 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 29 0.61 SPBC336.07 |sfc3||transcription factor TFIIIC complex subunit Sf... 29 0.61 SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizos... 28 1.1 SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.4 SPAC25B8.11 |||transcription factor|Schizosaccharomyces pombe|ch... 27 2.4 SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit ... 27 3.2 SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces ... 27 3.2 SPAC21E11.07 ||SPAC2C4.01|glycine cleavage T-protein|Schizosacch... 27 3.2 SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomy... 26 4.3 SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 26 4.3 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 26 5.6 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 26 5.6 SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce... 25 7.5 SPBC428.02c |eca39|SPBC582.12c|branched chain amino acid aminotr... 25 7.5 SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 25 7.5 SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 9.9 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 31.5 bits (68), Expect = 0.11 Identities = 37/151 (24%), Positives = 63/151 (41%), Gaps = 3/151 (1%) Frame = +2 Query: 98 LKSQLEGFNTSCLRDVDTNEKIVLPSAEDVAKEKQHNAL--LQGVELFQPSSLRKTETIE 271 L S++ N+ L+ D EK + +E E++ N+L Q +E SSL+ +T+E Sbjct: 135 LNSEISNQNSLILQKKDELEKSIQRCSE---LEEKINSLESAQSIEQEVISSLKDDKTVE 191 Query: 272 -KNILPNAIDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLN 448 KN +P +T+ G+ S + K + + K IEA E + Sbjct: 192 TKNDVPEVSRPSTDTI------GVSSALSKKKKKRNRKNQKKKSTKQNIEATTENDALSE 245 Query: 449 GIENFDPTKLKHTETCEKNPLPTKDVIEQEK 541 I D K+ +E + D E+E+ Sbjct: 246 SISTPDIQKVAQSEDVDAEQDTVADSKEEER 276 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 30.3 bits (65), Expect = 0.26 Identities = 16/55 (29%), Positives = 20/55 (36%) Frame = +1 Query: 358 PAEAHRDSGEEPASGQRCYRSGEGKEQIPERHRELRSH*AEAHGNVREEPAPHKG 522 P H + GE + GE P +H EL H H E+ HKG Sbjct: 313 PPPMHHEPGEHMPPPPMHHEPGEHMPPPPFKHHELEEHEGPEHHRGPEDKEHHKG 367 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 29.1 bits (62), Expect = 0.61 Identities = 14/43 (32%), Positives = 22/43 (51%) Frame = +2 Query: 206 NALLQGVELFQPSSLRKTETIEKNILPNAIDVATEKTQKSLFD 334 + L EL Q +SLR + I N ++D+ + +K LFD Sbjct: 1766 SGLADDSELVQTASLRAAKMIVNNYATKSVDLLLPELEKGLFD 1808 >SPBC336.07 |sfc3||transcription factor TFIIIC complex subunit Sfc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 1339 Score = 29.1 bits (62), Expect = 0.61 Identities = 24/103 (23%), Positives = 48/103 (46%), Gaps = 6/103 (5%) Frame = +2 Query: 257 TETIEKNILPNAI-DVATEKTQKSLFDGIEKFDSSQLK-HTETQEKNPLPDKDAIEAEKE 430 T IEK P + DV + +++ K +++K H E+ E P+ ++ + EKE Sbjct: 763 TPEIEKQSTPEILKDVDVDFLKRNTSLSRRKSMPAEIKRHKESSETKPVDKEEVKKNEKE 822 Query: 431 KNKFL----NGIENFDPTKLKHTETCEKNPLPTKDVIEQEKSA 547 K+ + +E+ P H T +K+P+ + +++ A Sbjct: 823 KDDPMRLAQQLLESLAPDFALHENTQQKSPVEKPKKLRKDRYA 865 >SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 28.3 bits (60), Expect = 1.1 Identities = 19/84 (22%), Positives = 41/84 (48%), Gaps = 2/84 (2%) Frame = +2 Query: 224 VELFQPSSLR-KTETIEKNILPNAIDVATEKTQKSLFDGIEKFDSSQLKHTETQEK-NPL 397 ++ FQ + L + E ++K ++ + E ++ LF ++ +++ T+E NP+ Sbjct: 402 IQSFQNAILEGEREDVDKKVVEFLNPLVLELQERLLFVVQTAIETDIQRYSPTEEDLNPI 461 Query: 398 PDKDAIEAEKEKNKFLNGIENFDP 469 D ++ + EK + N E DP Sbjct: 462 ADDKSLLFDLEKLRLNNDEEKLDP 485 >SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 27.1 bits (57), Expect = 2.4 Identities = 23/53 (43%), Positives = 27/53 (50%) Frame = -3 Query: 231 SSTPCSKALCCFSFATSSADGSTIFSLVSTSRRQLVLKPSS*LFRSVATLGRS 73 SS+ S L SF TS A S I SLVS+S +Q +S S ATL S Sbjct: 58 SSSSSSSPLSSSSF-TSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSS 109 >SPAC25B8.11 |||transcription factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 654 Score = 27.1 bits (57), Expect = 2.4 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +2 Query: 392 PLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPL 511 P D D I+ +E F NG+ +FD +K+ T K+P+ Sbjct: 159 PTCDSD-IDLIREYFSFENGVCSFDNMTVKYVSTSSKSPV 197 >SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit Bdp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 507 Score = 26.6 bits (56), Expect = 3.2 Identities = 16/44 (36%), Positives = 22/44 (50%) Frame = +2 Query: 416 EAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTKDVIEQEKSA 547 E EKE K ENF+ + + E E+ L +EQEK+A Sbjct: 445 EMEKELQKIR---ENFEEERRRAIEVAEQRQLIVNHELEQEKNA 485 >SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 528 Score = 26.6 bits (56), Expect = 3.2 Identities = 17/44 (38%), Positives = 22/44 (50%) Frame = -3 Query: 195 SFATSSADGSTIFSLVSTSRRQLVLKPSS*LFRSVATLGRSFRE 64 SF SSA S+ +SRR V+ S FR+VA + RE Sbjct: 15 SFTNSSAQQLFSKSIAHSSRRNFVISSSCTKFRNVAIQRNAKRE 58 >SPAC21E11.07 ||SPAC2C4.01|glycine cleavage T-protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 325 Score = 26.6 bits (56), Expect = 3.2 Identities = 16/49 (32%), Positives = 28/49 (57%), Gaps = 2/49 (4%) Frame = +1 Query: 514 HKGRH*AREISLNHYFITVTRKCI-SLVSPYFNIDVSQ-IDLRRPLQVL 654 HKG + +E+++ Y+ VTRK I + P + + SQ I+ PL ++ Sbjct: 215 HKGCYLGQELTVRTYYTGVTRKRIFPFIIPNYEDNPSQVIEPSAPLSIV 263 >SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 274 Score = 26.2 bits (55), Expect = 4.3 Identities = 28/118 (23%), Positives = 51/118 (43%), Gaps = 2/118 (1%) Frame = +2 Query: 83 KVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVAKEKQHNALLQGVELFQPSSLRKTE 262 K D+K+ L+GFN L D EKI+ + E+ + + +S K Sbjct: 82 KFEGDIKNCLDGFNE--LNDPIVQEKILRAFEQGHVDEEDEERCRK---MASDASEEKDR 136 Query: 263 TIEKNILPNAIDVAT-EKTQKS-LFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKE 430 IE+ L + + + +KS +EK KH ++++P P + +E ++E Sbjct: 137 KIEEGELTSEEEKEPIQDLRKSHKRKSVEKSSVPNKKHKAERKRSPSPKIEILEDDEE 194 >SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 26.2 bits (55), Expect = 4.3 Identities = 22/85 (25%), Positives = 38/85 (44%) Frame = +2 Query: 11 SSSKIY*FTMACSVSDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVA 190 S SK+ +A S + ++ D P+ +T S L+ F+T + + + VLPS D Sbjct: 26 SPSKVCRDNIALSEHNVITVLDTPQRSTQCDSLLKSFSTPLV----SGSEDVLPSPRDAL 81 Query: 191 KEKQHNALLQGVELFQPSSLRKTET 265 ++ + L SS + T T Sbjct: 82 NITNKKSVTDNLLLSLTSSNQSTNT 106 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 25.8 bits (54), Expect = 5.6 Identities = 16/67 (23%), Positives = 28/67 (41%) Frame = +2 Query: 341 EKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTK 520 + FD +K +EK L + AE EK++ N +E+ + + N K Sbjct: 74 KSFDEELVKFHNLEEKLQLTETKCRNAESEKSRVENELEDLTSSLFEEANRMVAN--ARK 131 Query: 521 DVIEQEK 541 + + EK Sbjct: 132 ETVASEK 138 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 25.8 bits (54), Expect = 5.6 Identities = 22/127 (17%), Positives = 51/127 (40%), Gaps = 2/127 (1%) Frame = +2 Query: 149 TNEKIVLPSAEDVAKEKQHNALLQGVELFQPSSLRKTETIEKNILPNAIDVATEKTQKSL 328 T E+ + E++ + Q L +E + ++ E +++ ++ + + L Sbjct: 869 TTERAIALDKEEILRRTQER--LANIEDSFSETKQQNENLQRESA--SLKQINNELESEL 924 Query: 329 FDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCE--K 502 + K ++ + E +EK L +KD ++ + E K + E C+ + Sbjct: 925 LEKTSKVETLLSEQNELKEKLSLEEKDLLDTKGELESLRENNATVLSEKAEFNEQCKSLQ 984 Query: 503 NPLPTKD 523 + TKD Sbjct: 985 ETIVTKD 991 >SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 25.4 bits (53), Expect = 7.5 Identities = 10/27 (37%), Positives = 16/27 (59%) Frame = +2 Query: 425 KEKNKFLNGIENFDPTKLKHTETCEKN 505 K + K L G ++ DP L+ + CE+N Sbjct: 307 KMQEKELEGSDSIDPILLEKIDVCEEN 333 >SPBC428.02c |eca39|SPBC582.12c|branched chain amino acid aminotransferase Eca39|Schizosaccharomyces pombe|chr 2|||Manual Length = 380 Score = 25.4 bits (53), Expect = 7.5 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +2 Query: 317 QKSLFDGIEKFDSSQLKHTETQEKNPLPD 403 Q + G + DSS +K T +E PLP+ Sbjct: 3 QTAALHGPKPMDSSHIKVTNVKELKPLPE 31 >SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pombe|chr 1|||Manual Length = 1325 Score = 25.4 bits (53), Expect = 7.5 Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = +2 Query: 281 LPNAIDV-ATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPD 403 +PN + + +T QKSL D E D++ LK + + LPD Sbjct: 75 VPNYLIIGSTLDLQKSLIDADENVDANVLKDSASIRHVSLPD 116 >SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 398 Score = 25.0 bits (52), Expect = 9.9 Identities = 21/71 (29%), Positives = 37/71 (52%) Frame = +2 Query: 62 PSLKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVAKEKQHNALLQGVELFQP 241 P K L K+ +L+S L+ SCL+D T+ K ++ +E ++ + L + + F Sbjct: 14 PGNKCLAKL-NELESILKDAKKSCLKDPTTSMKELVACSEKT--QQVFDDLKRTEKKFHT 70 Query: 242 SSLRKTETIEK 274 S R +T+EK Sbjct: 71 SLNRFGKTLEK 81 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,646,716 Number of Sequences: 5004 Number of extensions: 55023 Number of successful extensions: 216 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 216 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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