BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0543 (636 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_29189| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7 SB_25692| Best HMM Match : I-set (HMM E-Value=2e-20) 27 9.7 SB_10606| Best HMM Match : I-set (HMM E-Value=0) 27 9.7 >SB_29189| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 725 Score = 27.5 bits (58), Expect = 9.7 Identities = 20/60 (33%), Positives = 28/60 (46%) Frame = -1 Query: 471 EEG*LARGAKSEKTLNIHAI*ITSAT*CSFITVNSVNNPTIKQSNAKPKNQYVHITYKRQ 292 + G L R K T I A+ +S T S++ P + KPKNQ +H YK+Q Sbjct: 253 QNGNLQRNLKHNPTKKIQALKRSSPT--------SLSYPQKDWLSKKPKNQDIHTRYKQQ 304 >SB_25692| Best HMM Match : I-set (HMM E-Value=2e-20) Length = 104 Score = 27.5 bits (58), Expect = 9.7 Identities = 13/27 (48%), Positives = 20/27 (74%), Gaps = 1/27 (3%) Frame = +2 Query: 209 SSDV-KLNCTYQLAFCVNNASSTLNIM 286 SSD+ + CT + AF +NA+ST+N+M Sbjct: 77 SSDIGRYTCTARNAFGKDNANSTVNVM 103 >SB_10606| Best HMM Match : I-set (HMM E-Value=0) Length = 872 Score = 27.5 bits (58), Expect = 9.7 Identities = 13/27 (48%), Positives = 20/27 (74%), Gaps = 1/27 (3%) Frame = +2 Query: 209 SSDV-KLNCTYQLAFCVNNASSTLNIM 286 SSD+ + CT + AF +NA+ST+N+M Sbjct: 735 SSDIGRYTCTARNAFGKDNANSTVNVM 761 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,321,337 Number of Sequences: 59808 Number of extensions: 315827 Number of successful extensions: 525 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 469 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 525 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1596754500 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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