Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0541
(683 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53850.2 68418.m06691 haloacid dehalogenase-like hydrolase fa... 182 3e-46
At5g53850.1 68418.m06692 haloacid dehalogenase-like hydrolase fa... 182 3e-46
At5g53850.3 68418.m06693 haloacid dehalogenase-like hydrolase fa... 165 2e-41
At1g06650.2 68414.m00705 2-oxoglutarate-dependent dioxygenase, p... 29 2.9
At1g06650.1 68414.m00704 2-oxoglutarate-dependent dioxygenase, p... 29 2.9
>At5g53850.2 68418.m06691 haloacid dehalogenase-like hydrolase
family protein low similarity to enolase-phosphatase E-1
enzyme [Klebsiella oxytoca] GI:401712; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 507
Score = 182 bits (442), Expect = 3e-46
Identities = 99/206 (48%), Positives = 124/206 (60%), Gaps = 14/206 (6%)
Frame = +1
Query: 106 LIPELCNQFYHLGWVTGTGGGISIKQGDK--------IYIAPSGVQKERMKANDLFVQT- 258
L+ ELC FY GWV+GTGG I++K D I ++PSGVQKERM+ D+++ +
Sbjct: 27 LVTELCRHFYTQGWVSGTGGSITMKVHDASIPKPEQLIVMSPSGVQKERMQPEDMYILSA 86
Query: 259 ---IXXXXXXXXXXXXXXXXSQCTPLFMLAYRMRNAGAVIHTHSPHAVRCTLLYD--KVF 423
I + C PLFM AY MRNAGAVIH+H + T+L K F
Sbjct: 87 NGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMRNAGAVIHSHGMESCLVTMLNPQAKEF 146
Query: 424 EITHQEMIKGIKDTSLGRYLRYDEKLVVPIIENTPFEKDLAGSLEEALKEYPGTSAVLVR 603
ITH EMIKGI+ YDE LVVPIIENT +E +L SL +A++ YP +AVLVR
Sbjct: 147 RITHMEMIKGIQGHGY-----YDE-LVVPIIENTAYENELTDSLTKAIEAYPKATAVLVR 200
Query: 604 RHGVYVWGDTWQQAKTMTECYDYLFE 681
HGVY+WGD+W AKT ECY YLF+
Sbjct: 201 NHGVYIWGDSWIHAKTQAECYHYLFD 226
>At5g53850.1 68418.m06692 haloacid dehalogenase-like hydrolase
family protein low similarity to enolase-phosphatase E-1
enzyme [Klebsiella oxytoca] GI:401712; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 402
Score = 182 bits (442), Expect = 3e-46
Identities = 99/206 (48%), Positives = 124/206 (60%), Gaps = 14/206 (6%)
Frame = +1
Query: 106 LIPELCNQFYHLGWVTGTGGGISIKQGDK--------IYIAPSGVQKERMKANDLFVQT- 258
L+ ELC FY GWV+GTGG I++K D I ++PSGVQKERM+ D+++ +
Sbjct: 27 LVTELCRHFYTQGWVSGTGGSITMKVHDASIPKPEQLIVMSPSGVQKERMQPEDMYILSA 86
Query: 259 ---IXXXXXXXXXXXXXXXXSQCTPLFMLAYRMRNAGAVIHTHSPHAVRCTLLYD--KVF 423
I + C PLFM AY MRNAGAVIH+H + T+L K F
Sbjct: 87 NGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMRNAGAVIHSHGMESCLVTMLNPQAKEF 146
Query: 424 EITHQEMIKGIKDTSLGRYLRYDEKLVVPIIENTPFEKDLAGSLEEALKEYPGTSAVLVR 603
ITH EMIKGI+ YDE LVVPIIENT +E +L SL +A++ YP +AVLVR
Sbjct: 147 RITHMEMIKGIQGHGY-----YDE-LVVPIIENTAYENELTDSLTKAIEAYPKATAVLVR 200
Query: 604 RHGVYVWGDTWQQAKTMTECYDYLFE 681
HGVY+WGD+W AKT ECY YLF+
Sbjct: 201 NHGVYIWGDSWIHAKTQAECYHYLFD 226
>At5g53850.3 68418.m06693 haloacid dehalogenase-like hydrolase
family protein low similarity to enolase-phosphatase E-1
enzyme [Klebsiella oxytoca] GI:401712; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 418
Score = 165 bits (401), Expect = 2e-41
Identities = 99/222 (44%), Positives = 124/222 (55%), Gaps = 30/222 (13%)
Frame = +1
Query: 106 LIPELCNQFYHLGWVTGTGGGISIKQGDK--------IYIAPSGVQKERMKANDLFV--- 252
L+ ELC FY GWV+GTGG I++K D I ++PSGVQKERM+ D+++
Sbjct: 27 LVTELCRHFYTQGWVSGTGGSITMKVHDASIPKPEQLIVMSPSGVQKERMQPEDMYILSA 86
Query: 253 -QTIXXXXXXXXXXXXXXXXSQCTPLFM----------------LAYRMRNAGAVIHTHS 381
+I + C PLFM AY MRNAGAVIH+H
Sbjct: 87 NGSIISTPSPKPYPNKPPKCTDCAPLFMKNRRLSLGKRLRKLGYKAYEMRNAGAVIHSHG 146
Query: 382 PHAVRCTLL--YDKVFEITHQEMIKGIKDTSLGRYLRYDEKLVVPIIENTPFEKDLAGSL 555
+ T+L K F ITH EMIKGI+ YDE LVVPIIENT +E +L SL
Sbjct: 147 MESCLVTMLNPQAKEFRITHMEMIKGIQGHGY-----YDE-LVVPIIENTAYENELTDSL 200
Query: 556 EEALKEYPGTSAVLVRRHGVYVWGDTWQQAKTMTECYDYLFE 681
+A++ YP +AVLVR HGVY+WGD+W AKT ECY YLF+
Sbjct: 201 TKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFD 242
>At1g06650.2 68414.m00705 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967); contains
Pfam profile: PF00671 Iron/Ascorbate oxidoreductase
family
Length = 369
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = -2
Query: 280 ILNLHHQLFGQTDHWLSCVPSELLKVQCKFCLLVLLIYHHPCQSPIL 140
++NL LF L P+ L + C LL+L Y+ PC P L
Sbjct: 188 VMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDL 234
>At1g06650.1 68414.m00704 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967); contains
Pfam profile: PF00671 Iron/Ascorbate oxidoreductase
family
Length = 314
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = -2
Query: 280 ILNLHHQLFGQTDHWLSCVPSELLKVQCKFCLLVLLIYHHPCQSPIL 140
++NL LF L P+ L + C LL+L Y+ PC P L
Sbjct: 188 VMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDL 234
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,030,768
Number of Sequences: 28952
Number of extensions: 284884
Number of successful extensions: 716
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 687
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 705
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -