BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0537 (599 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3G9.16c |bet5|SPAC688.15|TRAPP complex subunit Bet5 |Schizos... 80 3e-16 SPBC3B9.12 |||TRAPP complex subunit Trs23 |Schizosaccharomyces p... 67 2e-12 SPBC11G11.04 |trs20||TRAPP complex subunit Trs20 |Schizosaccharo... 30 0.30 SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 26 3.7 SPCC569.06 |||S. pombe specific multicopy membrane protein famil... 26 4.8 >SPAC3G9.16c |bet5|SPAC688.15|TRAPP complex subunit Bet5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 79.8 bits (188), Expect = 3e-16 Identities = 48/152 (31%), Positives = 85/152 (55%), Gaps = 17/152 (11%) Frame = +2 Query: 176 MTIYNLYIFDRYGTLLYYGEWNRSKQSGM----------SIEEE-GKLMYGMLFSIKSFV 322 MTIY YI+ R ++ W S ++ M SIE++ KL++G++FS+++ V Sbjct: 1 MTIYAFYIYSRKCECVFAHRWKPSDRNSMETLVSQLEQNSIEDDMEKLIFGVVFSLRNMV 60 Query: 323 SKISPLDPKDGFSHYKTSKYTLHCLETPSGLKFVMNTDNQAQGVRDLLKKIYAEIYVKYM 502 KI+ +D F Y TSKY LH ETP+ L+ + T+ + + +L++IY +YV+++ Sbjct: 61 KKITA--DQDQFMSYTTSKYKLHFYETPTNLRLIFITNPKIDSLTHVLQQIYTTLYVEFV 118 Query: 503 IRNPLCEMGEP------IVSDLFKSKLDLFIK 580 +++PL P I ++F+ LD F++ Sbjct: 119 VKHPLYTHVPPSAEEGGINCEIFRITLDRFVR 150 >SPBC3B9.12 |||TRAPP complex subunit Trs23 |Schizosaccharomyces pombe|chr 2|||Manual Length = 132 Score = 67.3 bits (157), Expect = 2e-12 Identities = 37/132 (28%), Positives = 69/132 (52%) Frame = +2 Query: 182 IYNLYIFDRYGTLLYYGEWNRSKQSGMSIEEEGKLMYGMLFSIKSFVSKISPLDPKDGFS 361 ++ L I +R G+L++ E+ S + E ++ G + + + ++ISPL G Sbjct: 1 MHALIIINRAGSLIFQREFGSSPTA--LTPNEYLVLAGTIHGVHAISTQISPLPGSSGIQ 58 Query: 362 HYKTSKYTLHCLETPSGLKFVMNTDNQAQGVRDLLKKIYAEIYVKYMIRNPLCEMGEPIV 541 + + +H L+T +G+KFV+ T+ + R L+K Y E+Y Y+++NP + PI Sbjct: 59 LLEAGTFNMHILQTHTGMKFVLFTEKKTTNARLQLQKFY-ELYSDYVLKNPFYTLEMPIK 117 Query: 542 SDLFKSKLDLFI 577 LF +L +I Sbjct: 118 CQLFDEQLKRYI 129 >SPBC11G11.04 |trs20||TRAPP complex subunit Trs20 |Schizosaccharomyces pombe|chr 2|||Manual Length = 136 Score = 29.9 bits (64), Expect = 0.30 Identities = 16/56 (28%), Positives = 34/56 (60%), Gaps = 1/56 (1%) Frame = +2 Query: 401 TPSGLKFVMNTDNQ-AQGVRDLLKKIYAEIYVKYMIRNPLCEMGEPIVSDLFKSKL 565 TPS ++F++ NQ A ++ ++++ E+Y+K ++ +P + +PI S F K+ Sbjct: 75 TPSNMRFMLLHQNQSADNIKLFFQELH-ELYIKTLM-SPFYQPNQPIRSQAFDLKV 128 >SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 2344 Score = 26.2 bits (55), Expect = 3.7 Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 1/54 (1%) Frame = -1 Query: 224 TIKYHIDRICINCKLSFFILSLEYTVSRTKKITNFHKIN-IPDYKNNNINIFDL 66 TI Y +DR C+ C I+SL S K +T+ + N + D++ + I ++ Sbjct: 1098 TIDY-LDRNCVTCSDKHVIISLLLVSSWEKFLTSNNYSNYLADFETTHAQIMEM 1150 >SPCC569.06 |||S. pombe specific multicopy membrane protein family 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 478 Score = 25.8 bits (54), Expect = 4.8 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -1 Query: 218 KYHIDRICINCKLSFFILS 162 K H+ ++C+ C SFFIL+ Sbjct: 240 KLHLVQVCLFCWFSFFILN 258 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,461,686 Number of Sequences: 5004 Number of extensions: 52205 Number of successful extensions: 150 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 262236260 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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