Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0529
(687 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 159 3e-40
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 133 3e-32
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 54 3e-08
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 30 0.27
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 29 0.63
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 28 1.1
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 28 1.1
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 27 1.9
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 27 3.4
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 4.4
SPBP19A11.04c |mor2|cps12|morphogenesis protein Mor2|Schizosacch... 26 5.9
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 159 bits (386), Expect = 3e-40
Identities = 82/163 (50%), Positives = 111/163 (68%), Gaps = 3/163 (1%)
Frame = +2
Query: 206 GRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV---NPTLSAPLEDVVNSI 376
G+ TLIPGDG+G E AV E+FK A++P++FE + + N + L + + S+
Sbjct: 19 GKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSL 78
Query: 377 AVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDVDCIII 556
NK+ +KGIL TP F G + N+ LR LD+YA++V +K++P K RH +VD II
Sbjct: 79 KRNKVGLKGILFTP-FEKGGHT-SFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAII 136
Query: 557 REQTEGEYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAV 685
RE TEGEYS LEH+SVPGVVE LKIIT KS+RIA+FAFD+A+
Sbjct: 137 RENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFAL 179
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 133 bits (321), Expect = 3e-32
Identities = 69/174 (39%), Positives = 105/174 (60%), Gaps = 2/174 (1%)
Frame = +2
Query: 167 APFGALQPRATKEGRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV--NPT 340
A G + G T+I GDG+GPE+ +V+ +FKAA +P+++E + N T
Sbjct: 31 AAAGTYEGVKNANGNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGT 90
Query: 341 LSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNV 520
+ P +D S+ NK+ +KG LATP G + ++N+ LR L+ANV S+
Sbjct: 91 TTIP-DDAKESVRKNKVALKGPLATP--IGKGHV-SMNLTLRRTFGLFANVRPCVSITGY 146
Query: 521 KCRHQDVDCIIIREQTEGEYSALEHESVPGVVECLKIITAAKSERIAKFAFDYA 682
K + +V+ ++IRE TEGEYS +EHE +PGVV+ +K+IT A SER+ ++AF YA
Sbjct: 147 KTPYDNVNTVLIRENTEGEYSGIEHEVIPGVVQSIKLITRAASERVIRYAFQYA 200
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 53.6 bits (123), Expect = 3e-08
Identities = 53/171 (30%), Positives = 81/171 (47%), Gaps = 13/171 (7%)
Frame = +2
Query: 209 RIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFE-SFFFSEVNP-------TLSAPLEDV 364
RI LIP DG+G E+V A + + + ++P + F F +++ T A E
Sbjct: 6 RIVLGLIPADGIGKEVVPAARRLME--NLPAKHKLKFDFIDLDAGWGTFERTGKALPERT 63
Query: 365 VNSIAVN-KICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDV 541
V + + G + +P G + + LR + LYANV VKSL K + V
Sbjct: 64 VERLKTECNAALFGAVQSPTHKVAGYSSPI-VALRKKMGLYANVRPVKSLDGAK--GKPV 120
Query: 542 DCIIIREQTEGEYSALEH--ESVPG--VVECLKIITAAKSERIAKFAFDYA 682
D +I+RE TE Y E ++ PG V E ++ I+ S +I K AF+ A
Sbjct: 121 DLVIVRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIGKMAFEIA 171
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 30.3 bits (65), Expect = 0.27
Identities = 24/101 (23%), Positives = 47/101 (46%), Gaps = 8/101 (7%)
Frame = +2
Query: 215 KCTLIPGDGVGPELVYAVQEVFKAA-----SIPVDFESFFFSEVN-PTLSAPLED-VVNS 373
K ++PGD +GPE+V + EV K + ++FE + PL D V +
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVKA 64
Query: 374 IAVNKICIKGILATPDFSHTG-ELQTLNMKLRNALDLYANV 493
+ G + P++++ + +KLR ++ ++AN+
Sbjct: 65 CLEADGVLLGAVGGPEWTNPNCRPEQGLLKLRKSMGVWANL 105
>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
Pms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 794
Score = 29.1 bits (62), Expect = 0.63
Identities = 25/83 (30%), Positives = 39/83 (46%)
Frame = +2
Query: 248 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 427
P A+Q++ S P+D + F ++N +LS +D+V S A+ K K I D S
Sbjct: 533 PSKTAALQKLKFFQSRPLDGLNKFSKKINISLSGVQKDIVRSDALLKFSNK-IGVVHDIS 591
Query: 428 HTGELQTLNMKLRNALDLYANVV 496
+ LN+ + A L VV
Sbjct: 592 DENQEDHLNLTVHKADFLRMRVV 614
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 28.3 bits (60), Expect = 1.1
Identities = 16/70 (22%), Positives = 31/70 (44%)
Frame = +2
Query: 296 PVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNAL 475
P F S N L D + ++ +KI I +LAT + + ++ T +L
Sbjct: 245 PASFTSSRNKRSNERLFGMTSDSLQTVTPDKIAILDLLATQSIASSTDMLTAFRELTQDF 304
Query: 476 DLYANVVHVK 505
+YA+ + ++
Sbjct: 305 PIYAHYLSIQ 314
>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 685
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2
Query: 179 LQMVHNIHFSPLLPNLCVH 123
LQ H++H SPL P CVH
Sbjct: 261 LQGTHSVHGSPLKPEDCVH 279
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.5 bits (58), Expect = 1.9
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = -3
Query: 391 NLVNSDRVYDVFEWCTKCWIHFRKEKGFKVHW 296
N VN+ VY +F WI+ R FK+ W
Sbjct: 269 NYVNTVIVYPIFVIFVFVWIYMRHYLNFKIMW 300
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 26.6 bits (56), Expect = 3.4
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -3
Query: 394 TNLVNSDRVYDVFEWCTKCW-IHFRKEKGFKVHWNAG 287
T L++ + ++++F CTK W +H +K+ K++ + G
Sbjct: 355 TPLLSQNDLWNIFFLCTKFWSLHSKKQDILKLYSDLG 391
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 26.2 bits (55), Expect = 4.4
Identities = 16/54 (29%), Positives = 24/54 (44%)
Frame = +2
Query: 323 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLY 484
S++N A LED N +C++ +A S LQ +K R+ L Y
Sbjct: 541 SQLNVFTKASLEDKQKKEKYNLLCVRSCIAIDSISWISSLQ--GVKFRSKLMAY 592
>SPBP19A11.04c |mor2|cps12|morphogenesis protein
Mor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2196
Score = 25.8 bits (54), Expect = 5.9
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = -3
Query: 205 FLCCPRLQGSKWCITYIFLRCYRTCVYTLSNMLRSLHNSSKNIP 74
FL P +Q + Y+ LRCY + + ++++LH SS+ +P
Sbjct: 1000 FLSTPNVQADE---KYLKLRCYFSQLLEKVLLVQNLHPSSEVLP 1040
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,930,474
Number of Sequences: 5004
Number of extensions: 64612
Number of successful extensions: 196
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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