BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0525 (624 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_37033| Best HMM Match : Annexin (HMM E-Value=0) 137 6e-33 SB_47771| Best HMM Match : Annexin (HMM E-Value=0) 93 6e-22 SB_26806| Best HMM Match : Annexin (HMM E-Value=0) 94 1e-19 SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19) 91 5e-19 SB_7681| Best HMM Match : Annexin (HMM E-Value=0) 66 2e-11 SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25) 57 1e-08 SB_32965| Best HMM Match : Kazal_1 (HMM E-Value=3.4e-19) 30 1.8 SB_56182| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.4 SB_34906| Best HMM Match : Cadherin (HMM E-Value=0) 28 7.1 >SB_37033| Best HMM Match : Annexin (HMM E-Value=0) Length = 287 Score = 137 bits (332), Expect = 6e-33 Identities = 64/126 (50%), Positives = 90/126 (71%) Frame = +3 Query: 195 GTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISDIKSETSGKFEDLLIALLTPLPKF 374 G D K+++ +L R+N QR RI+LE+KT++G+DLI D+KSE G FED +IAL+TP ++ Sbjct: 101 GCDNKALMYLLCSRTNSQRQRISLEYKTMFGRDLIKDLKSEVGGYFEDTVIALMTPPAEY 160 Query: 375 YAKELHEAMVGIGTDEGVLIEVMCTMSNYEIHSIEQAYTAIYGKILEDDIRGDTSGNFNR 554 A L +A+ G+GTDE VLIEV+ T +N EI +I AY ++ + LE DI GDTSG F + Sbjct: 161 DATLLRKAIKGLGTDEAVLIEVLTTRTNDEIIAIRNAYNTLFSRDLEKDIAGDTSGKFKK 220 Query: 555 LMTSLC 572 + SLC Sbjct: 221 FLISLC 226 Score = 72.1 bits (169), Expect = 3e-13 Identities = 35/71 (49%), Positives = 49/71 (69%) Frame = +3 Query: 159 DAGVLRKAMKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISDIKSETSGKFED 338 DA +LRKA+KG GTDE +I+VLT R+N++ + I + TL+ +DL DI +TSGKF+ Sbjct: 161 DATLLRKAIKGLGTDEAVLIEVLTTRTNDEIIAIRNAYNTLFSRDLEKDIAGDTSGKFKK 220 Query: 339 LLIALLTPLPK 371 LI+L L K Sbjct: 221 FLISLCNALYK 231 >SB_47771| Best HMM Match : Annexin (HMM E-Value=0) Length = 529 Score = 93.1 bits (221), Expect(2) = 6e-22 Identities = 48/93 (51%), Positives = 63/93 (67%) Frame = +3 Query: 123 TVFPVNPFNPREDAGVLRKAMKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLIS 302 TV F+ + A L+KAMKGFG+DE++II+VL + EQRL IA FK Y KDL++ Sbjct: 295 TVVAAENFDAGDCAEKLKKAMKGFGSDEEAIIEVLQSCNLEQRLAIAQSFKQQYDKDLVN 354 Query: 303 DIKSETSGKFEDLLIALLTPLPKFYAKELHEAM 401 ++KSE SGKFED ++ALL P + A LH AM Sbjct: 355 ELKSELSGKFEDAIVALLLPPEQLDANALHGAM 387 Score = 54.8 bits (126), Expect = 5e-08 Identities = 32/103 (31%), Positives = 59/103 (57%), Gaps = 2/103 (1%) Frame = +3 Query: 156 EDAGVLRKAMKGFGTDEKSIIQVLT-KRSNEQRLR-IALEFKTLYGKDLISDIKSETSGK 329 +D L+KA++ +D+K+ +V+ S+E +L+ ++ E+ Y KDL+ + + + Sbjct: 47 DDITKLQKAIEK--SDKKTATEVIVCNASDEGKLKKLSDEYNKRYEKDLVEQLDGKVADD 104 Query: 330 FEDLLIALLTPLPKFYAKELHEAMVGIGTDEGVLIEVMCTMSN 458 + + +LL P + + L+ AM G+GT E LIE +CT SN Sbjct: 105 VHEAVTSLLLPATQADVRSLYYAMKGLGTKEAQLIETLCTRSN 147 Score = 40.3 bits (90), Expect = 0.001 Identities = 20/64 (31%), Positives = 39/64 (60%) Frame = +3 Query: 378 AKELHEAMVGIGTDEGVLIEVMCTMSNYEIHSIEQAYTAIYGKILEDDIRGDTSGNFNRL 557 A++L +AM G G+DE +IEV+ + + + +I Q++ Y K L ++++ + SG F Sbjct: 308 AEKLKKAMKGFGSDEEAIIEVLQSCNLEQRLAIAQSFKQQYDKDLVNELKSELSGKFEDA 367 Query: 558 MTSL 569 + +L Sbjct: 368 IVAL 371 Score = 33.9 bits (74), Expect = 0.11 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%) Frame = +3 Query: 135 VNPFNPREDAGVLRKA-MKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISDIK 311 VN EDA L +A K +GTDE ++LT RS+ Q + ++ + D++ I Sbjct: 398 VNEDQAYEDAKNLYEAGEKKWGTDESVFTKILTCRSDLQLRALYQAYQHVAKCDILETID 457 Query: 312 SETSGKFEDLLIAL 353 E +G + D + A+ Sbjct: 458 DELTGDYHDAVKAI 471 Score = 32.3 bits (70), Expect = 0.33 Identities = 18/36 (50%), Positives = 20/36 (55%) Frame = +3 Query: 159 DAGVLRKAMKGFGTDEKSIIQVLTKRSNEQRLRIAL 266 D L AMKG GT E +I+ L RSN LR AL Sbjct: 120 DVRSLYYAMKGLGTKEAQLIETLCTRSNTGDLRDAL 155 Score = 28.7 bits (61), Expect(2) = 6e-22 Identities = 17/57 (29%), Positives = 32/57 (56%), Gaps = 5/57 (8%) Frame = +3 Query: 378 AKELHEA-MVGIGTDEGVLIEVMCTMSNYEIHSIEQAYTAIYG----KILEDDIRGD 533 AK L+EA GTDE V +++ S+ ++ ++ QAY + + ++D++ GD Sbjct: 407 AKNLYEAGEKKWGTDESVFTKILTCRSDLQLRALYQAYQHVAKCDILETIDDELTGD 463 >SB_26806| Best HMM Match : Annexin (HMM E-Value=0) Length = 829 Score = 93.9 bits (223), Expect = 1e-19 Identities = 52/153 (33%), Positives = 88/153 (57%), Gaps = 2/153 (1%) Frame = +3 Query: 156 EDAGVLRKAMKGFGTDEKSIIQVLTKR-SNEQRLR-IALEFKTLYGKDLISDIKSETSGK 329 +D L+KA+ +D+K+ ++ S+E +L+ + E+ Y KDL+ + + + Sbjct: 444 DDITKLQKAIDK--SDKKTTTAIIVSNASDEGKLKKLNDEYNKRYKKDLVEQLDGKVAID 501 Query: 330 FEDLLIALLTPLPKFYAKELHEAMVGIGTDEGVLIEVMCTMSNYEIHSIEQAYTAIYGKI 509 + + +LL P K + L+ AM G+GT E LIE++CT SN EI I+Q Y YG+ Sbjct: 502 VHEAVTSLLLPATKADVRSLYYAMKGLGTKEAQLIEILCTRSNAEIQEIKQEYNKKYGRS 561 Query: 510 LEDDIRGDTSGNFNRLMTSLCVGNRSEDFTVDQ 608 L DD++ +TSG+F L+ +L G+R E V++ Sbjct: 562 LTDDMKSETSGDFENLLETLLKGSRDESTDVNK 594 Score = 70.9 bits (166), Expect = 8e-13 Identities = 53/169 (31%), Positives = 81/169 (47%), Gaps = 14/169 (8%) Frame = +3 Query: 153 REDAGVLRKAMKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISDIKSETSGKF 332 + D L AMKG GT E +I++L RSN + I E+ YG+ L D+KSETSG F Sbjct: 515 KADVRSLYYAMKGLGTKEAQLIEILCTRSNAEIQEIKQEYNKKYGRSLTDDMKSETSGDF 574 Query: 333 EDLLIALL-------TPLPKFYAKELHEAMV--GI---GTDEGVLIEVMCTMSNYEIHSI 476 E+LL LL T + K + A+V G+ GTDE I ++ S+ + ++ Sbjct: 575 ENLLETLLKGSRDESTDVNKELVTKDAAALVSAGVARWGTDEDQFITILSQRSSAHVQAV 634 Query: 477 EQAYTAIYGKI-LEDDIRGDTSGNF-NRLMTSLCVGNRSEDFTVDQNRA 617 Y + G + L D +R + G+ + L+ + G D+ A Sbjct: 635 LAEYKTLSGGVSLADTVRDEMKGDLRDALLVLISYGQDPSQAAADKLNA 683 Score = 39.9 bits (89), Expect = 0.002 Identities = 17/31 (54%), Positives = 23/31 (74%) Frame = +3 Query: 267 EFKTLYGKDLISDIKSETSGKFEDLLIALLT 359 E++ YG L D+K ETSG FEDLL+AL++ Sbjct: 94 EYQKKYGCSLEDDVKEETSGDFEDLLVALMS 124 Score = 38.3 bits (85), Expect = 0.005 Identities = 35/115 (30%), Positives = 55/115 (47%), Gaps = 2/115 (1%) Frame = +3 Query: 231 KRSNEQRLRIALEFKTLYGKDLISDIKSETSGKFEDLLIALLTPLPKFYAKELHEAMVG- 407 K+ E ++ A++ K L K L D K + D L L ++LHEA+ Sbjct: 19 KKIKELVMKAAVDKKKL--KKLGDDYKKQYKQDLTDALDGKLE-------EDLHEAVAAM 69 Query: 408 -IGTDEGVLIEVMCTMSNYEIHSIEQAYTAIYGKILEDDIRGDTSGNFNRLMTSL 569 + +E + M N I SI++ Y YG LEDD++ +TSG+F L+ +L Sbjct: 70 LLPGNEADARTLYFAMKN--IDSIKEEYQKKYGCSLEDDVKEETSGDFEDLLVAL 122 Score = 31.5 bits (68), Expect = 0.57 Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 1/51 (1%) Frame = +3 Query: 156 EDAGVLRKA-MKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISD 305 +DA L A + +GT+E I +LT+RS+ + E++TL G ++D Sbjct: 772 QDAAALVSAGVARWGTEEDQFITILTQRSSAHVQAVLAEYRTLSGGVTLAD 822 >SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19) Length = 146 Score = 91.5 bits (217), Expect = 5e-19 Identities = 42/87 (48%), Positives = 59/87 (67%) Frame = +3 Query: 198 TDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISDIKSETSGKFEDLLIALLTPLPKFY 377 T+E +I +L RSN QR+ I +KT+YGKDL++D+KSE SG E+ L+A++ P + Sbjct: 2 TNEAELIGILANRSNAQRVEIRKRYKTMYGKDLMNDLKSELSGNLEECLLAMMEPSVLYD 61 Query: 378 AKELHEAMVGIGTDEGVLIEVMCTMSN 458 AK L M G GTDE LI+++CT SN Sbjct: 62 AKCLRRGMRGAGTDEETLIDILCTRSN 88 Score = 37.1 bits (82), Expect = 0.012 Identities = 16/52 (30%), Positives = 29/52 (55%) Frame = +3 Query: 414 TDEGVLIEVMCTMSNYEIHSIEQAYTAIYGKILEDDIRGDTSGNFNRLMTSL 569 T+E LI ++ SN + I + Y +YGK L +D++ + SGN + ++ Sbjct: 2 TNEAELIGILANRSNAQRVEIRKRYKTMYGKDLMNDLKSELSGNLEECLLAM 53 Score = 36.7 bits (81), Expect = 0.015 Identities = 15/29 (51%), Positives = 22/29 (75%) Frame = +3 Query: 159 DAGVLRKAMKGFGTDEKSIIQVLTKRSNE 245 DA LR+ M+G GTDE+++I +L RSN+ Sbjct: 61 DAKCLRRGMRGAGTDEETLIDILCTRSNQ 89 >SB_7681| Best HMM Match : Annexin (HMM E-Value=0) Length = 426 Score = 66.5 bits (155), Expect = 2e-11 Identities = 46/153 (30%), Positives = 78/153 (50%), Gaps = 1/153 (0%) Frame = +3 Query: 114 SRPTVFPVNPFNPREDAGVLRKA-MKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGK 290 +R T V+ ++A L KA K +GTDE VL RS Q E+ + + Sbjct: 2 NRDTNMTVDMAKATKEAQDLYKAGEKKWGTDESRFNVVLASRSFPQLQATFNEYIKISQR 61 Query: 291 DLISDIKSETSGKFEDLLIALLTPLPKFYAKELHEAMVGIGTDEGVLIEVMCTMSNYEIH 470 D+++ I E SG + L P +++A L ++M G GTD+ +LI V+ + S ++ Sbjct: 62 DIMNSIDREMSGDLKAGLQCARNPA-EYFADRLWKSMKGAGTDDSLLIRVVVSRSEVDLV 120 Query: 471 SIEQAYTAIYGKILEDDIRGDTSGNFNRLMTSL 569 I+ ++ Y K + I GD SG++ +L+ +L Sbjct: 121 EIKASFLQKYHKTVYKMIEGDCSGDYKKLLMAL 153 Score = 47.6 bits (108), Expect = 8e-06 Identities = 28/73 (38%), Positives = 41/73 (56%), Gaps = 1/73 (1%) Frame = +3 Query: 147 NPRED-AGVLRKAMKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISDIKSETS 323 NP E A L K+MKG GTD+ +I+V+ RS + I F Y K + I+ + S Sbjct: 84 NPAEYFADRLWKSMKGAGTDDSLLIRVVVSRSEVDLVEIKASFLQKYHKTVYKMIEGDCS 143 Query: 324 GKFEDLLIALLTP 362 G ++ LL+AL +P Sbjct: 144 GDYKKLLMALPSP 156 >SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25) Length = 803 Score = 57.2 bits (132), Expect = 1e-08 Identities = 31/106 (29%), Positives = 62/106 (58%), Gaps = 4/106 (3%) Frame = +3 Query: 153 REDAGVLRKAMKGFGTD--EKSIIQVLTKRSNEQRL--RIALEFKTLYGKDLISDIKSET 320 +EDA L +A++G ++ + +II +L + +E+ R+ ++K + +D++ ++ Sbjct: 347 KEDADELHQAIQGMVSEGNKDAIIDILARNCDERSKLDRLKDKYKDQHDQDILEALQPHL 406 Query: 321 SGKFEDLLIALLTPLPKFYAKELHEAMVGIGTDEGVLIEVMCTMSN 458 +D + + + F AK LH+A+ G+GTDE +LIE++CT N Sbjct: 407 GDDVKDPVESFFSSSSAFDAKCLHDAIKGLGTDEDLLIEIICTRKN 452 Score = 54.0 bits (124), Expect = 9e-08 Identities = 30/87 (34%), Positives = 49/87 (56%), Gaps = 1/87 (1%) Frame = +3 Query: 201 DEKSIIQVLTKRSNEQRL-RIALEFKTLYGKDLISDIKSETSGKFEDLLIALLTPLPKFY 377 D++ I+ +L + S + ++ ++ ++K LY KDL ++KS + L +L Sbjct: 21 DKERIMGILGRTSEKDKMNKLEDKYKDLYKKDLNEELKSVLDEEIMKALESLAEEAAMKD 80 Query: 378 AKELHEAMVGIGTDEGVLIEVMCTMSN 458 AK L+EA G GTDE LI ++CT SN Sbjct: 81 AKTLYEATKGFGTDEDALISILCTRSN 107 Score = 39.5 bits (88), Expect = 0.002 Identities = 32/128 (25%), Positives = 62/128 (48%), Gaps = 3/128 (2%) Frame = +3 Query: 153 REDAGVLRKA-MKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTLYGKDLISDIKSETSGK 329 ++DA L +A + +GTDE + I+V T+RS Q + E+K L ++ I E G Sbjct: 123 KKDAKDLMEAGVASWGTDEGAFIRVFTQRSFTQISAMCAEYKKLTKVEIEESIDKEMDGD 182 Query: 330 FEDLLIAL--LTPLPKFYAKELHEAMVGIGTDEGVLIEVMCTMSNYEIHSIEQAYTAIYG 503 + + L ++ P + + +A V G G + +++ M+ +++ + +Y Sbjct: 183 MQKAFLTLVKISRDPTGFQGDKLKAAVKDG-HLGAVAKILLPMNKAKLNEVSASYKKDTK 241 Query: 504 KILEDDIR 527 L DDI+ Sbjct: 242 HELADDIQ 249 Score = 36.7 bits (81), Expect = 0.015 Identities = 16/30 (53%), Positives = 22/30 (73%) Frame = +3 Query: 156 EDAGVLRKAMKGFGTDEKSIIQVLTKRSNE 245 +DA L +A KGFGTDE ++I +L RSN+ Sbjct: 79 KDAKTLYEATKGFGTDEDALISILCTRSNK 108 Score = 32.7 bits (71), Expect = 0.25 Identities = 15/41 (36%), Positives = 23/41 (56%) Frame = +3 Query: 159 DAGVLRKAMKGFGTDEKSIIQVLTKRSNEQRLRIALEFKTL 281 DA L A+KG GTDE +I+++ R N + I++ L Sbjct: 425 DAKCLHDAIKGLGTDEDLLIEIICTRKNTVSILISIVLSPL 465 >SB_32965| Best HMM Match : Kazal_1 (HMM E-Value=3.4e-19) Length = 69 Score = 29.9 bits (64), Expect = 1.8 Identities = 14/40 (35%), Positives = 23/40 (57%) Frame = -3 Query: 220 WIIDFSSVPKPFMALRRTPASSRGLNGFTGKTVGLDC*LG 101 ++ DF ++P+ +R PA + + G GKT G +C LG Sbjct: 11 FLFDFKALPQVDKCVRPCPAINDPVCGTDGKTYGNECMLG 50 >SB_56182| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 525 Score = 28.3 bits (60), Expect = 5.4 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = +3 Query: 84 EGKIMFPNQQSRPTVFPVN 140 EGKI FPN++ R V P N Sbjct: 419 EGKIQFPNEEFREIVLPCN 437 >SB_34906| Best HMM Match : Cadherin (HMM E-Value=0) Length = 3922 Score = 27.9 bits (59), Expect = 7.1 Identities = 24/83 (28%), Positives = 40/83 (48%), Gaps = 5/83 (6%) Frame = +3 Query: 51 LISCFISKDIGEGKIMFPNQQSRPTVFPVNPFNPREDAGVLRKAMKGFGTDEKS-----I 215 +I I++D + I ++ R +V FN + GVL A K T KS I Sbjct: 1406 MIGAVIARDQDQDDI----EKVRYSVVSGTEFNVNTETGVLTVA-KDLSTGAKSEYSMEI 1460 Query: 216 IQVLTKRSNEQRLRIALEFKTLY 284 + +SN+ R+R+ +E+K +Y Sbjct: 1461 RATDSTKSNDARVRVNVEYKNMY 1483 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,056,762 Number of Sequences: 59808 Number of extensions: 388740 Number of successful extensions: 1273 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 1203 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1272 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1548368000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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