BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0488
(502 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 31 0.097
SPAC22E12.10c |etp1|cox15|mitochondrial type I [2Fe-2S] ferredox... 29 0.30
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 26 2.8
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 26 3.7
SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 26 3.7
SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo... 25 4.8
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 25 6.4
SPBC4B4.02c |nca2||mitochondrial protein Nca2 |Schizosaccharomyc... 25 6.4
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 6.4
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 25 8.4
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 31.1 bits (67), Expect = 0.097
Identities = 26/98 (26%), Positives = 45/98 (45%), Gaps = 5/98 (5%)
Frame = +1
Query: 217 NVFHVEHHSRVPEYHLVQLTHHLARRNIPTSHPANSNSADSGKTPHLKTEKVTKGGYKAP 396
+V ++E + V HLV + ++P S PA+ + +TP + T + G P
Sbjct: 183 HVGYLEMYQYVIARHLVAICKQYNLIHLPRSLPADVIESAKSETPAMNTN--IEQGVTQP 240
Query: 397 SLLNDEMF-----VEAKKRIEDVDIMGDPNSTVSVDFK 495
SL+N E + + K + +I D N+ +VD K
Sbjct: 241 SLVNTEQLSHQQSISSTKSSKLDEISADNNAQSAVDNK 278
>SPAC22E12.10c |etp1|cox15|mitochondrial type I [2Fe-2S] ferredoxin
Etp1/ cytochrome oxidase cofactor Cox15,
fusion|Schizosaccharomyces pombe|chr 1|||Manual
Length = 631
Score = 29.5 bits (63), Expect = 0.30
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +1
Query: 181 KIHEHMTIDELRNVFHVEHHSRV 249
K++ HMT+DE +N+F E RV
Sbjct: 161 KLNSHMTVDEFKNIFFWEWFHRV 183
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 26.2 bits (55), Expect = 2.8
Identities = 22/96 (22%), Positives = 38/96 (39%)
Frame = +1
Query: 133 AFVILCSFITTQSLNNKIHEHMTIDELRNVFHVEHHSRVPEYHLVQLTHHLARRNIPTSH 312
A +L I T N + ++D + + V + R+ + H + + L
Sbjct: 449 AVTVLMGIIHTSDRNLALLVGRSLDAQKFIHDVTYDHRLRDSH--REIYQLQGTGYRPFL 506
Query: 313 PANSNSADSGKTPHLKTEKVTKGGYKAPSLLNDEMF 420
AN N++ + K H + E G +PS L D F
Sbjct: 507 RANDNASINNKNHHKELEDNESGTRISPSTLGDTSF 542
>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 25.8 bits (54), Expect = 3.7
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +1
Query: 319 NSNSADSGKTPHLKTEKVT 375
N N DS KTPH + +K+T
Sbjct: 947 NDNLIDSIKTPHTELQKIT 965
>SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein
Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 968
Score = 25.8 bits (54), Expect = 3.7
Identities = 10/26 (38%), Positives = 12/26 (46%)
Frame = +2
Query: 266 FSSHITWPEGIYPLLTQRTRTAPIQE 343
F+ H W YP + TRT P E
Sbjct: 746 FTKHNNWEASTYPSAPKNTRTYPTAE 771
>SPAC4H3.11c |ppc89|mug127|spindle pole body protein
Ppc89|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 25.4 bits (53), Expect = 4.8
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +1
Query: 301 PTSHPANSNSADSGKTPHLKTEKVTKGGYKAPSLLNDEM 417
PT+ P N S TP+L + K T YKA + L +++
Sbjct: 191 PTT-PWRRNGFRSKTTPNLNSGKETPSSYKASARLMEQL 228
>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
Msa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 533
Score = 25.0 bits (52), Expect = 6.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +3
Query: 240 QQSTRVPPGSAHTSLGQKEYTHFSP 314
Q +PPG+ TS+ Q Y +SP
Sbjct: 487 QSMATLPPGAVPTSIPQSYYPIYSP 511
>SPBC4B4.02c |nca2||mitochondrial protein Nca2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 573
Score = 25.0 bits (52), Expect = 6.4
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = -2
Query: 216 PKLIYCHVLVDLVVERLCRDE*AEYHERREPVRRHGRLHDCPRVLI 79
P LI+C+V++ V + ++ ERR+ R+ L R+L+
Sbjct: 458 PSLIFCYVIIRYVKANIFNNDTLSRAERRQRFRQ--SLRAAERILV 501
>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 535
Score = 25.0 bits (52), Expect = 6.4
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 300 THFSPSELEQRRFRKDPPFKNRKSH 374
T F+ S +RR+R PP +N K H
Sbjct: 470 TAFTRSREWRRRYRVAPPAENEKPH 494
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 24.6 bits (51), Expect = 8.4
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = -3
Query: 497 TLKSTLTVLLGSPMISTSSIRFFASTN 417
TLK+TL + +P TSS RFF + N
Sbjct: 1220 TLKTTLLMYGENPDEPTSSARFFNNLN 1246
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,200,765
Number of Sequences: 5004
Number of extensions: 46636
Number of successful extensions: 145
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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