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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0477
         (674 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g25240.1 68415.m03020 serpin, putative / serine protease inhi...    52   4e-07
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi...    46   2e-05
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi...    43   2e-04
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi...    42   3e-04
At1g64030.1 68414.m07252 serpin family protein / serine protease...    40   0.002
At1g64010.1 68414.m07250 serpin, putative / serine protease inhi...    40   0.002
At1g62170.1 68414.m07013 serpin family protein / serine protease...    39   0.004
At2g14540.1 68415.m01628 serpin family protein / serine protease...    38   0.005
At2g35580.1 68415.m04357 serpin family protein / serine protease...    37   0.014
At3g11080.1 68416.m01339 disease resistance family protein conta...    29   2.8  
At2g18860.1 68415.m02198 syntaxin family protein similar to Synt...    29   3.8  
At1g72270.1 68414.m08355 expressed protein                             29   3.8  
At2g40720.1 68415.m05023 pentatricopeptide (PPR) repeat-containi...    28   6.6  
At5g13350.1 68418.m01538 auxin-responsive GH3 family protein sim...    27   8.7  
At4g24640.1 68417.m03528 invertase/pectin methylesterase inhibit...    27   8.7  
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r...    27   8.7  
At1g53360.1 68414.m06049 F-box family protein-related similar to...    27   8.7  
At1g03000.1 68414.m00271 AAA-type ATPase family protein contains...    27   8.7  

>At2g25240.1 68415.m03020 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 324

 Score = 52.0 bits (119), Expect = 4e-07
 Identities = 26/68 (38%), Positives = 39/68 (57%), Gaps = 4/68 (5%)
 Frame = +3

Query: 435 VYVDSVLHNVKITVNEFGTEAVAATAGILA----RSAEQFYADSPFYIFIRNEKTKLVTF 602
           +YV S+LH   I V+E GTEA A + G+++    R    F AD PF   +R +K+ ++ F
Sbjct: 255 LYVSSILHKACIEVDEEGTEAAAVSVGVVSCTSFRRNPDFVADRPFLFTVREDKSGVILF 314

Query: 603 SAVVYDPT 626
              V DP+
Sbjct: 315 MGQVLDPS 322


>At3g45220.1 68416.m04880 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 393

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 24/68 (35%), Positives = 36/68 (52%), Gaps = 3/68 (4%)
 Frame = +3

Query: 435 VYVDSVLHNVKITVNEFGTEAVAATAGILARSA---EQFYADSPFYIFIRNEKTKLVTFS 605
           ++V +V H   I V+E GTEA A +   + +       F AD PF   +R EK+ ++ F 
Sbjct: 325 LFVSNVFHKACIEVDEEGTEAAAVSVASMTKDMLLMGDFVADHPFLFTVREEKSGVILFM 384

Query: 606 AVVYDPTI 629
             V DP+I
Sbjct: 385 GQVLDPSI 392


>At2g26390.1 68415.m03167 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 389

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 22/68 (32%), Positives = 35/68 (51%), Gaps = 4/68 (5%)
 Frame = +3

Query: 435 VYVDSVLHNVKITVNEFGTEAVAATAGILARSA----EQFYADSPFYIFIRNEKTKLVTF 602
           ++V S++H   I V+E GTEA A +  I+          F AD PF   +R + + ++ F
Sbjct: 320 LHVSSIIHKACIEVDEEGTEAAAVSVAIMMPQCLMRNPDFVADHPFLFTVREDNSGVILF 379

Query: 603 SAVVYDPT 626
              V DP+
Sbjct: 380 IGQVLDPS 387


>At1g47710.1 68414.m05302 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 391

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 25/68 (36%), Positives = 33/68 (48%), Gaps = 7/68 (10%)
 Frame = +3

Query: 441 VDSVLHNVKITVNEFGTEAVAATAGI-------LARSAEQFYADSPFYIFIRNEKTKLVT 599
           V ++ H   I VNE GTEA AA+AG+       +      F AD PF + +    T +V 
Sbjct: 322 VSNIFHKACIEVNEEGTEAAAASAGVIKLRGLLMEEDEIDFVADHPFLLVVTENITGVVL 381

Query: 600 FSAVVYDP 623
           F   V DP
Sbjct: 382 FIGQVVDP 389


>At1g64030.1 68414.m07252 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311, serpin [Triticum
           aestivum] GI:871551; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 385

 Score = 39.9 bits (89), Expect = 0.002
 Identities = 26/69 (37%), Positives = 34/69 (49%), Gaps = 9/69 (13%)
 Frame = +3

Query: 447 SVLHNVKITVNEFGTEAVAATA-GILARSAE--------QFYADSPFYIFIRNEKTKLVT 599
           S+ H   + ++E G EA AATA G    S +         F AD PF   IR EKT  V 
Sbjct: 304 SMYHKACVEIDEEGAEAAAATADGDCGCSLDFVEPPKKIDFVADHPFLFLIREEKTGTVL 363

Query: 600 FSAVVYDPT 626
           F   ++DP+
Sbjct: 364 FVGQIFDPS 372


>At1g64010.1 68414.m07250 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 185

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 22/58 (37%), Positives = 30/58 (51%), Gaps = 5/58 (8%)
 Frame = +3

Query: 468 ITVNEFGTEAVAATA-----GILARSAEQFYADSPFYIFIRNEKTKLVTFSAVVYDPT 626
           + ++E G EA+AATA     G        F AD PF   IR +KT  V F   ++DP+
Sbjct: 125 VEIDEEGAEAIAATAVVGGFGCAFVKRIDFVADHPFLFMIREDKTGTVLFVGQIFDPS 182


>At1g62170.1 68414.m07013 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           GI:9937311 from [Cucurbita maxima]; contains Pfam
           profile PF00079: Serpin (serine protease inhibitor)
          Length = 433

 Score = 38.7 bits (86), Expect = 0.004
 Identities = 23/59 (38%), Positives = 30/59 (50%), Gaps = 6/59 (10%)
 Frame = +3

Query: 468 ITVNEFGTEAVAATA------GILARSAEQFYADSPFYIFIRNEKTKLVTFSAVVYDPT 626
           I ++E GTEAV  TA      G        F AD PF   IR E+T  V F+  ++DP+
Sbjct: 374 IEIDEKGTEAVTFTAFRSAYLGCALVKPIDFVADHPFLFLIREEQTGTVLFAGQIFDPS 432


>At2g14540.1 68415.m01628 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 407

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 8/61 (13%)
 Frame = +3

Query: 468 ITVNEFGTEAVAATAGILA--------RSAEQFYADSPFYIFIRNEKTKLVTFSAVVYDP 623
           I ++E GTEA AAT  ++         +    F AD PF   IR +KT  + F+  ++DP
Sbjct: 338 IEIDEEGTEAAAATTVVVVTGSCLWEPKKKIDFVADHPFLFLIREDKTGTLLFAGQIFDP 397

Query: 624 T 626
           +
Sbjct: 398 S 398


>At2g35580.1 68415.m04357 serpin family protein / serine protease
           inhibitor family protein similar to protein zx [Hordeum
           vulgare subsp. vulgare] GI:19071, serpin [Triticum
           aestivum] GI:1885350; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 374

 Score = 36.7 bits (81), Expect = 0.014
 Identities = 24/70 (34%), Positives = 35/70 (50%), Gaps = 6/70 (8%)
 Frame = +3

Query: 435 VYVDSVLHNVKITVNEFGTEAVAATA--GILAR----SAEQFYADSPFYIFIRNEKTKLV 596
           V +  ++H   I V+E G++A AA A  GI  R        F AD PF   ++  ++ LV
Sbjct: 303 VPLSMIMHKSCIEVDEVGSKAAAAAAFRGIGCRRPPPEKHDFVADHPFLFIVKEYRSGLV 362

Query: 597 TFSAVVYDPT 626
            F   V DP+
Sbjct: 363 LFLGQVMDPS 372


>At3g11080.1 68416.m01339 disease resistance family protein contains
           leucine rich-repeat domains Pfam:PF00560,
           INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
           pimpinellifolium] gi|1184077|gb|AAC15780
          Length = 943

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 28/100 (28%), Positives = 47/100 (47%), Gaps = 5/100 (5%)
 Frame = -2

Query: 499 TASVPNSLTVILTLCKTESTYTPGLGKLFNASFNI-----LDCS*PNLDLITTSFSFLSS 335
           T ++PN+++++  L   E++     G L ++ FNI     LD S   L+  T  F  +SS
Sbjct: 306 TGTIPNNISLLSNLMDFEASNNAFTGTLPSSLFNIPPLIRLDLSDNQLN-GTLHFGNISS 364

Query: 334 DESIIKAKLASSGLNIDLIPTAFSALSRLTLLSKSILGNR 215
             ++    + S+   I  IP + S    LTL   S L  +
Sbjct: 365 PSNLQYLIIGSNNF-IGTIPRSLSRFVNLTLFDLSHLNTQ 403


>At2g18860.1 68415.m02198 syntaxin family protein similar to
           Syntaxin 61 (AtSYP61) (Osmotic stess-sensitive mutant 1)
           (Swiss-Prot:Q946Y7) [Arabidopsis thaliana]
          Length = 296

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 12/47 (25%), Positives = 30/47 (63%), Gaps = 1/47 (2%)
 Frame = +3

Query: 228 MDLESNVNLDKALKAVGIKSMF-SPDEANFALMIDSSDDKKENEVVI 365
           +D+E  V   +A K+VG++ ++ SP+  +  +++   D+++E E ++
Sbjct: 183 IDIEERVTPGQAEKSVGLRRIWSSPNFNSLRIIVPGGDNEEEKETLV 229


>At1g72270.1 68414.m08355 expressed protein
          Length = 2777

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = -2

Query: 340  SSDESIIKAKLASSGLNIDLIPTAFSALSRLTLLSKSILGN 218
            ++ ESI+K   +   ++ DL  T+FS L  +  L K+ LGN
Sbjct: 1195 ATPESILKNFASVMAVSWDLYGTSFSFLLSIIFLEKNFLGN 1235


>At2g40720.1 68415.m05023 pentatricopeptide (PPR) repeat-containing
           protein contains INTERPRO:IPR002885 PPR repeats
          Length = 860

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 11/34 (32%), Positives = 20/34 (58%)
 Frame = +3

Query: 78  NDSTTVMYALKPRLPMKLSLFDLMKKLDYNKIDE 179
           +D T V ++     PMK  +F+++ +L  N +DE
Sbjct: 823 SDRTNVFFSGGSSSPMKAEIFNVLNRLKSNMVDE 856


>At5g13350.1 68418.m01538 auxin-responsive GH3 family protein
           similar to auxin-responsive GH3 product [Glycine max]
           GI:18591; contains Pfam profile PF03321: GH3
           auxin-responsive promoter
          Length = 587

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = +3

Query: 540 FYADSPFYIFIRNEKTKLVTFSAVVYDPTI*KQFTTNKLL 659
           FY +SP + F+  +K  L    A  Y+  + K  T  KLL
Sbjct: 415 FYNNSPHFRFVGRQKVVLSLHMANTYEEDLLKAVTNAKLL 454


>At4g24640.1 68417.m03528 invertase/pectin methylesterase inhibitor
           family protein annotation temporarily based on
           supporting cDNA gi|4097527|gb|U63898.1|U63898
          Length = 187

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = +3

Query: 264 LKAVGIKSMFSPDEANFALMIDSSDDKKENEVV 362
           LK+  +K   SPD AN+ +   S   ++ NE+V
Sbjct: 112 LKSANVKLKASPDTANYDVRASSDSMRRVNELV 144


>At3g59820.1 68416.m06675 calcium-binding mitochondrial
           protein-related contains weak similarity to
           Calcium-binding mitochondrial protein Anon-60Da
           (Swiss-Prot:P91927) [Drosophila melanogaster]
          Length = 755

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 18/75 (24%), Positives = 35/75 (46%)
 Frame = +3

Query: 117 LPMKLSLFDLMKKLDYNKIDELINKMNERKAVVRFPKMDLESNVNLDKALKAVGIKSMFS 296
           LP +  + +  +KL+Y ++ E + K  E K      ++      + DKAL+ + I +   
Sbjct: 506 LPSEDPVSERRRKLEYLEMQEELIKEEEEKEEEELTRIKDVKGGDEDKALQEMTIPTASE 565

Query: 297 PDEANFALMIDSSDD 341
             E   A +++  DD
Sbjct: 566 AQEQARARVLEQQDD 580


>At1g53360.1 68414.m06049 F-box family protein-related similar to
           hypothetical protein GI:4589954 from [Arabidopsis
           thaliana]; contains TIGRFAM TIGR01640: F-box protein
           interaction domain
          Length = 343

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 26/78 (33%), Positives = 36/78 (46%), Gaps = 1/78 (1%)
 Frame = +3

Query: 369 SRFGHEQSRMLKDALNSLPNPGVYV-DSVLHNVKITVNEFGTEAVAATAGILARSAEQFY 545
           SR G     +L+DA N   +  + +   ++HN +I V   GT       G +  S    Y
Sbjct: 244 SRNGELVLWVLEDAENHKWSKSICILPPIVHNSRI-VGVTGT-------GEIVFSPYACY 295

Query: 546 ADSPFYIFIRNEKTKLVT 599
             SPFYIF  N +TK  T
Sbjct: 296 MPSPFYIFFYNIQTKTCT 313


>At1g03000.1 68414.m00271 AAA-type ATPase family protein contains
           Pfam domain, PF00004: ATPase, AAA family ('A'TPases
           'A'ssociated with diverse cellular 'A'ctivities)
          Length = 941

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 26/95 (27%), Positives = 42/95 (44%), Gaps = 2/95 (2%)
 Frame = +3

Query: 318 LMIDSSDDKKENEVVIRSRFGHE-QSRMLKDALNSLPNPGVYVDSVLHNVKITVNEFGTE 494
           L+I S++  +     IR  F HE +   L D   S       +  V   + I+ +EF   
Sbjct: 508 LLIASAESTEGISPTIRRCFSHEIRMGSLNDEQRS-EMLSQSLQGVSQFLNISSDEFMKG 566

Query: 495 AVAATAGILARSAEQFYADSPFYIFIRNE-KTKLV 596
            V  T+G L R  +   AD+   ++I  E +TK +
Sbjct: 567 LVGQTSGFLPRDLQALVADAGANLYISQESETKKI 601


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,829,585
Number of Sequences: 28952
Number of extensions: 244056
Number of successful extensions: 753
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 729
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 747
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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