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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0460
         (759 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g12840.1 68414.m01491 vacuolar ATP synthase subunit C (VATC) ...   168   3e-42
At1g29340.1 68414.m03587 armadillo/beta-catenin repeat family pr...    31   0.83 
At1g14390.1 68414.m01706 leucine-rich repeat transmembrane prote...    31   0.83 
At5g50440.1 68418.m06246 Golgi SNARE protein membrin 12 (MEMB12)...    31   1.1  
At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to SP...    30   1.9  
At5g63720.1 68418.m07998 hypothetical protein                          29   3.4  
At2g18480.1 68415.m02153 mannitol transporter, putative similar ...    29   4.4  
At3g59180.1 68416.m06598 hypothetical protein contains a novel d...    28   5.9  
At2g25450.1 68415.m03048 2-oxoglutarate-dependent dioxygenase, p...    28   5.9  
At4g13540.1 68417.m02111 expressed protein                             28   7.7  

>At1g12840.1 68414.m01491 vacuolar ATP synthase subunit C (VATC) /
           V-ATPase C subunit / vacuolar proton pump C subunit
           (DET3) identical to vacuolar ATP synthase subunit C
           SP:Q9SDS7 from [Arabidopsis thaliana]
          Length = 375

 Score =  168 bits (409), Expect = 3e-42
 Identities = 88/209 (42%), Positives = 127/209 (60%), Gaps = 1/209 (0%)
 Frame = +3

Query: 135 TEYWVISAPGDKTCQQTWDTLNNATKSGNLSVN-YIFPIPDLKVGTLDQLVGLSDDLGKL 311
           + YWV+S P   +    W+ L       +     Y F IP+L+VGTLD L+ L DDL K 
Sbjct: 3   SRYWVVSLPVKDSASSLWNRLQEQISKHSFDTPVYRFNIPNLRVGTLDSLLALGDDLLKS 62

Query: 312 DTFVEGVTRKVAQYLGEVLEDQRDKLHENLMANNSDLPTYLTRFQWDMAKYPIKQSLRNI 491
           ++FVEGV++K+ + + E LE         L  +   + +YLTRF WD AKYP    L+ +
Sbjct: 63  NSFVEGVSQKIRRQIEE-LERISGVESNALTVDGVPVDSYLTRFVWDEAKYPTMSPLKEV 121

Query: 492 ADIISKQVGQIDADLKVKSSAYNALKGNLHNLEKKQTGSLLTRNLADLVKKEHFILDSEY 671
            D I  QV +I+ DLKV+ + YN ++G L+ + +KQ+GSL  R+L++LVK E  I++SE+
Sbjct: 122 VDNIQSQVAKIEDDLKVRVAEYNNIRGQLNAINRKQSGSLAVRDLSNLVKPED-IVESEH 180

Query: 672 LTTLLVIVPKSMFNDWNANYEKITDMIVP 758
           L TLL +VPK    DW A YE +TD +VP
Sbjct: 181 LVTLLAVVPKYSQKDWLACYETLTDYVVP 209


>At1g29340.1 68414.m03587 armadillo/beta-catenin repeat family
           protein / U-box domain-containing protein contains Pfam
           domain, PF00514: Armadillo/beta-catenin-like repeats and
           Pfam, PF04564: U-box domain
          Length = 729

 Score = 31.1 bits (67), Expect = 0.83
 Identities = 23/96 (23%), Positives = 46/96 (47%), Gaps = 3/96 (3%)
 Frame = +3

Query: 282 VGLSDDLGKLDTFVEGVTRKVAQYLGEVLEDQRDKLHENLMA-NNSDLPTYLTRFQWDMA 458
           +GLSDD+ +    ++  +RK   Y+ +  E  R+  +  L    N  +P+ +    + + 
Sbjct: 162 LGLSDDIREQIELLQRQSRKARLYIDKNDESLRESFYSFLDGFENGKIPSSVDLRMFFVE 221

Query: 459 KYPIK--QSLRNIADIISKQVGQIDADLKVKSSAYN 560
           K  I+  +S R+  + + +Q+   D DL+   S  N
Sbjct: 222 KLGIRDSKSCRSEIEFLEEQIVNHDGDLEPTGSVIN 257


>At1g14390.1 68414.m01706 leucine-rich repeat transmembrane protein
           kinase, putative similar to putative receptor-like
           protein kinase GI:2947063 from [Arabidopsis thaliana]
          Length = 747

 Score = 31.1 bits (67), Expect = 0.83
 Identities = 19/58 (32%), Positives = 28/58 (48%)
 Frame = -3

Query: 757 GTIMSVIFS*LAFQSLNIDLGTMTRSVVRYSLSKMKCSFLTRSAKLRVNKLPVCFFSK 584
           G+I   + S  + Q+L++    ++ S+   SL   K   L  S  L   KLP CF SK
Sbjct: 239 GSIPRFLLSLPSLQNLSLAQNLLSGSLPNSSLCNSKLRILDVSRNLLTGKLPSCFSSK 296


>At5g50440.1 68418.m06246 Golgi SNARE protein membrin 12 (MEMB12)
           identical to Membrin 12 (AtMEMB12) (Golgi SNAP receptor
           complex member 2-2) (GI:27805575)(SP:Q9FK28)
           {Arabidopsis thaliana}; similar to Probable 27 kDa Golgi
           SNARE protein (Golgi SNAP receptor complex member 2)
           (SP:Q9SJL6) [Arabidopsis thaliana]
          Length = 219

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 24/86 (27%), Positives = 40/86 (46%)
 Frame = +2

Query: 461 VPHKAESAEYRRHHKQTGRTDRRGSEGQVLRLQRSQRKPTQFREETDRELVDPQLGRSSQ 640
           +P K++   +RR  +Q G      ++     + R+QRK  + +E  D       LGR S 
Sbjct: 65  IPVKSQRDLWRRKSEQVGEEAEYLNQSLEKYMWRNQRKMLEAKERAD------LLGRGSG 118

Query: 641 EGALHLGQRVPDHTPGHRAQVNVQRL 718
           EGA H+ Q   +   G  +  N +R+
Sbjct: 119 EGA-HILQIFDEEAQGMNSVKNSKRM 143


>At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to
           SP|P39904 SAC2 protein {Saccharomyces cerevisiae};
           contains Pfam profile PF04129: Vps52 / Sac2 family
          Length = 707

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 37/159 (23%), Positives = 62/159 (38%), Gaps = 11/159 (6%)
 Frame = +3

Query: 150 ISAPGDKTCQQTWDTLNNATKSGNLSVNYIFPIPDLKVGTLDQLVGLSDDLGKLDTFVEG 329
           I + GDK  +      NN  K    S+       D  V   DQ+      L +++T + G
Sbjct: 65  ILSSGDKLREYAKGVENNLRKVELDSIEDYIKESDNLVSLHDQIRDCDSILSQMETLLSG 124

Query: 330 VTRKVAQYLGEVLEDQRDKLHENL-MANNSDLPTYLTRFQWDMAKYP----------IKQ 476
              ++     ++   Q   +   L + N     + L +F  D+   P          + +
Sbjct: 125 FQEEIGSISSDIKILQEKSMDMGLRLKNRRVAESKLAKFVEDIIVPPKMIDVIVDGEVNE 184

Query: 477 SLRNIADIISKQVGQIDADLKVKSSAYNALKGNLHNLEK 593
                 +I+SK++  ++AD  VKSS   ALK     LEK
Sbjct: 185 EYMKTLEILSKKLKFVEADQAVKSS--KALKDVEPELEK 221


>At5g63720.1 68418.m07998 hypothetical protein 
          Length = 492

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +3

Query: 612 LTRNLADLVKKEHFILDSEYLTTLLVIVPKSMFN 713
           L + L D    EHF+  S  L T L + PK++ +
Sbjct: 42  LLKRLLDFASHEHFVTQSNLLATQLRVFPKTVLH 75


>At2g18480.1 68415.m02153 mannitol transporter, putative similar to
           mannitol transporter [Apium graveolens var. dulce]
           GI:12004316; contains Pfam profile PF00083: major
           facilitator superfamily protein
          Length = 508

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 18/57 (31%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
 Frame = -2

Query: 497 VGDIPQTLLYGVLSHVPLEASQISGEVT-VIGHQVFMELIALIFKYLSKVLGYLPGH 330
           + D    +L G+L+   L  S  +G+ + VIG +  + L A+IF   S ++GY P +
Sbjct: 56  INDTQIEVLAGILNLCALVGSLTAGKTSDVIGRRYTIALSAVIFLVGSVLMGYGPNY 112


>At3g59180.1 68416.m06598 hypothetical protein contains a novel
           domain with similarity to F-box domain;
          Length = 475

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +1

Query: 247 FLTLRWELWISWWGCQMIWAS 309
           FL + W LWI +W C+ I  S
Sbjct: 7   FLQVLWILWIEYWHCKAILLS 27


>At2g25450.1 68415.m03048 2-oxoglutarate-dependent dioxygenase,
           putative similar to 2A6 (GI:599622) and tomato ethylene
           synthesis regulatory protein E8 (SP|P10967)
          Length = 359

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 18/65 (27%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
 Frame = -2

Query: 518 SDLFAYDVGDIPQTLLYGVLSHVPL--EASQISGEVTVIGHQVFMELIALIFKYLSKVLG 345
           +DL+         TL   +   VP   +  ++ GE+ +   +  M+L  L+F+ LS+ LG
Sbjct: 135 ADLYESPAASWRDTLSCVMAPDVPKAQDLPEVCGEIMLEYSKEVMKLAELMFEILSEALG 194

Query: 344 YLPGH 330
             P H
Sbjct: 195 LSPNH 199


>At4g13540.1 68417.m02111 expressed protein
          Length = 210

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 10/35 (28%), Positives = 21/35 (60%)
 Frame = +3

Query: 501 ISKQVGQIDADLKVKSSAYNALKGNLHNLEKKQTG 605
           ++K V ++  ++K +      LKG ++ +EK+Q G
Sbjct: 133 VAKAVQELRKEVKARGETIETLKGRINLMEKQQNG 167


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,658,230
Number of Sequences: 28952
Number of extensions: 350083
Number of successful extensions: 1086
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1052
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1083
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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