BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0440 (718 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 239 3e-64 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 113 2e-26 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 77 2e-15 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 71 2e-13 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 67 2e-12 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 60 3e-10 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 38 0.002 SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 34 0.018 SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac... 32 0.071 SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 32 0.094 SPBC336.08 |spc24||spindle pole body protein Spc24|Schizosacchar... 29 0.88 SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 1.2 SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 28 1.2 SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 28 1.2 SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|c... 28 1.5 SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccha... 27 2.0 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 27 2.7 SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo... 27 2.7 SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 26 4.7 SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 8.2 SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 25 8.2 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 8.2 SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizos... 25 8.2 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 239 bits (585), Expect = 3e-64 Identities = 113/151 (74%), Positives = 134/151 (88%) Frame = +1 Query: 265 ESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEA 444 E DLY ++K L+R LE L++QE +IK++ ++LK+E + AQEEVKRIQSVPLVIGQFLEA Sbjct: 5 EEIDLYNRWKALERQLEMLDLQEGFIKEDCKSLKRELIRAQEEVKRIQSVPLVIGQFLEA 64 Query: 445 VDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQ 624 +DQNT IVGSTTGSNY VRILST+DRELLKPSASVAL +HSNALVD+LPPEADSSISML+ Sbjct: 65 IDQNTAIVGSTTGSNYVVRILSTLDRELLKPSASVALQRHSNALVDILPPEADSSISMLR 124 Query: 625 ADEKPDVQYSDXGGMDTQKQEIREAVELPLT 717 DE+PDV Y+D GG+D QKQE+REAVELPLT Sbjct: 125 PDERPDVSYADVGGLDVQKQEVREAVELPLT 155 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 113 bits (272), Expect = 2e-26 Identities = 57/144 (39%), Positives = 95/144 (65%), Gaps = 3/144 (2%) Frame = +1 Query: 295 KLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSV---PLVIGQFLEAVDQNTGI 465 K+QR+ + L ++EEY+++++R LK + QEE R+ + P+ +G E +D + I Sbjct: 71 KMQRIHDHLLMEEEYVQNQER-LKPQDERTQEERNRVDEIRGTPMSVGTLEEIIDDDHAI 129 Query: 466 VGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDV 645 V ST G YYV I+S +D+++L+P SV LH + ++V +L + D I++++ D+ P Sbjct: 130 V-STAGPEYYVSIMSFVDKDMLEPGCSVLLHHKAMSIVGLLLDDTDPMINVMKLDKAPTE 188 Query: 646 QYSDXGGMDTQKQEIREAVELPLT 717 Y+D GG+++Q QEI+EAVELPLT Sbjct: 189 SYADIGGLESQIQEIKEAVELPLT 212 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 77.4 bits (182), Expect = 2e-15 Identities = 40/150 (26%), Positives = 80/150 (53%) Frame = +1 Query: 265 ESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEA 444 E + KYK + + + ++ R+L K+Y ++++K +QSV +IG+ L+ Sbjct: 3 ERDTALEKYKSYLLQHREWDSKLKDLRFGNRDLVKKYDKTEDDIKSLQSVGQIIGEVLKQ 62 Query: 445 VDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQ 624 +D IV +++G Y V + +D+ L V+L + ++ +LP E D + + Sbjct: 63 LDSERFIVKASSGPRYVVGCRNNVDQSHLVQGVRVSLDMTTLTIMRILPREVDPLVYNMS 122 Query: 625 ADEKPDVQYSDXGGMDTQKQEIREAVELPL 714 ++ D+ ++ GG++ Q +E+RE +ELPL Sbjct: 123 IEDPGDISFAGVGGLNEQIRELREVIELPL 152 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 70.9 bits (166), Expect = 2e-13 Identities = 38/124 (30%), Positives = 67/124 (54%), Gaps = 1/124 (0%) Frame = +1 Query: 346 DEQRN-LKKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDR 522 + QRN L +EE++ +Q +G+ ++ + +N +V Y V I ID Sbjct: 42 EAQRNGLNARVRLLREEIQLLQEPGSYVGEVIKTMGKNKVLVKVHPEGKYVVDISPDIDI 101 Query: 523 ELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDXGGMDTQKQEIREAV 702 + +KP+ VAL S L+ +LP + D +S++ ++ PD Y GG++ Q +EI+E + Sbjct: 102 KEIKPNIRVALRNDSYQLIKILPNKVDPLVSLMMVEKIPDSTYEMVGGLEKQIKEIKEVI 161 Query: 703 ELPL 714 ELP+ Sbjct: 162 ELPV 165 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 67.3 bits (157), Expect = 2e-12 Identities = 38/169 (22%), Positives = 88/169 (52%), Gaps = 21/169 (12%) Frame = +1 Query: 271 EDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEAVD 450 ++L ++ + L+ ++ ++ + + + E+ + ++ QE++ + +P ++G +E +D Sbjct: 37 DELNSRTRLLENDIKVMKSEFQRLTHEKSTMLEKIKENQEKISNNKMLPYLVGNVVEILD 96 Query: 451 -------------QNT--------GIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHS 567 QN+ ++ ++T ++ ++ ++ E L P + ++K S Sbjct: 97 MQPDEVDVQESANQNSEATRVGKSAVIKTSTRQTIFLPLIGLVEPEELHPGDLIGVNKDS 156 Query: 568 NALVDVLPPEADSSISMLQADEKPDVQYSDXGGMDTQKQEIREAVELPL 714 ++D LP E DS + ++ DEKP +YSD GG+ Q +E+ EA+ LP+ Sbjct: 157 YLIIDKLPSEYDSRVKAMEVDEKPTERYSDIGGLSKQIEELFEAIVLPM 205 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 60.1 bits (139), Expect = 3e-10 Identities = 28/92 (30%), Positives = 50/92 (54%) Frame = +1 Query: 439 EAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISM 618 ++ ++N ++ + + V + + ++ V ++ A+ LPP+ D S++M Sbjct: 108 QSAEKNAYVINLKQIAKFVVSLGERVSPTDIEEGMRVGCDRNKYAIQLPLPPKIDPSVTM 167 Query: 619 LQADEKPDVQYSDXGGMDTQKQEIREAVELPL 714 +Q +EKPDV Y D GG Q + +RE VELPL Sbjct: 168 MQVEEKPDVTYGDVGGCKEQIERLREVVELPL 199 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 37.5 bits (83), Expect = 0.002 Identities = 15/29 (51%), Positives = 22/29 (75%) Frame = +1 Query: 631 EKPDVQYSDXGGMDTQKQEIREAVELPLT 717 E P+V +SD GG + KQ+++E+VE PLT Sbjct: 541 ESPNVHWSDIGGQEEVKQKLKESVEWPLT 569 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +1 Query: 643 VQYSDXGGMDTQKQEIREAVELP 711 V +S GG+ Q +IR+ VELP Sbjct: 276 VTFSSIGGLQAQIAQIRDIVELP 298 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 34.3 bits (75), Expect = 0.018 Identities = 12/28 (42%), Positives = 22/28 (78%) Frame = +1 Query: 631 EKPDVQYSDXGGMDTQKQEIREAVELPL 714 E P+V++ D GG++ K+E+RE V++P+ Sbjct: 490 EVPNVRWEDIGGLEEVKRELRETVQMPV 517 Score = 30.7 bits (66), Expect = 0.22 Identities = 14/25 (56%), Positives = 16/25 (64%) Frame = +1 Query: 640 DVQYSDXGGMDTQKQEIREAVELPL 714 +V Y D GG Q +IRE VELPL Sbjct: 220 EVGYDDIGGCRRQMAQIRELVELPL 244 >SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosaccharomyces pombe|chr 2|||Manual Length = 506 Score = 32.3 bits (70), Expect = 0.071 Identities = 14/52 (26%), Positives = 28/52 (53%), Gaps = 1/52 (1%) Frame = -2 Query: 477 GAANYACILINCLK-KLSNDKRNRLYALYFFLCMQIFFLQVSLFIFNVLFLY 325 G Y C+++ + ++N +N+ A FF C+ + F Q++L+ +F Y Sbjct: 182 GHFQYNCVMLGLVMYAIANLLKNQYVAATFFFCLALTFKQMALYFAPPIFFY 233 >SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 31.9 bits (69), Expect = 0.094 Identities = 14/50 (28%), Positives = 29/50 (58%) Frame = +1 Query: 565 SNALVDVLPPEADSSISMLQADEKPDVQYSDXGGMDTQKQEIREAVELPL 714 +N +D + S+++ EKP+V++ D G++ K+ ++E V LP+ Sbjct: 100 ANEALDSDAKKLRSALTSAILVEKPNVRWDDIAGLENAKEALKETVLLPI 149 >SPBC336.08 |spc24||spindle pole body protein Spc24|Schizosaccharomyces pombe|chr 2|||Manual Length = 198 Score = 28.7 bits (61), Expect = 0.88 Identities = 21/55 (38%), Positives = 26/55 (47%), Gaps = 2/55 (3%) Frame = +1 Query: 247 QSFDELESEDLYTKYKKLQRMLEFLEVQEEYIKD--EQRNLKKEYLHAQEEVKRI 405 QSF E E E TK+K L R LE E +K E +E L ++E RI Sbjct: 36 QSFREKELEGSQTKFKVLSRKLEISTQAMESLKQTAESSQHAEEILSREKEKFRI 90 >SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 608 Score = 28.3 bits (60), Expect = 1.2 Identities = 20/62 (32%), Positives = 33/62 (53%), Gaps = 3/62 (4%) Frame = +1 Query: 532 KPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQY-SDXGGMDTQKQE--IREAV 702 KPS++V+ S+A DV+ + S+I+ D K DV + + D Q +E I+E Sbjct: 244 KPSSNVSRGTPSSAHSDVIEISSSSAIARTSGDRKDDVLFDTSTQRRDNQTREMFIQEQA 303 Query: 703 EL 708 +L Sbjct: 304 QL 305 >SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 682 Score = 28.3 bits (60), Expect = 1.2 Identities = 12/27 (44%), Positives = 20/27 (74%) Frame = -2 Query: 408 LYALYFFLCMQIFFLQVSLFIFNVLFL 328 +++L+FFLCM+ FF +++ F LFL Sbjct: 360 IFSLFFFLCMRNFFPGFTIY-FPALFL 385 >SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 948 Score = 28.3 bits (60), Expect = 1.2 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = +1 Query: 637 PDVQYSDXGGMDTQKQEIREAVELPL 714 P V + D GG++ K +R+ ++LPL Sbjct: 650 PKVNWDDIGGLEEAKTVLRDTLQLPL 675 >SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 581 Score = 27.9 bits (59), Expect = 1.5 Identities = 19/66 (28%), Positives = 31/66 (46%) Frame = +1 Query: 493 YVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDXGGMD 672 +V L T + +A++ K D L + D + +Q+D KPDV+ SD MD Sbjct: 227 FVLFLFTFEETTFDRAAAID-RKQIQISNDQLTVKEDFDLKDMQSDIKPDVEKSD--AMD 283 Query: 673 TQKQEI 690 K ++ Sbjct: 284 DSKMKV 289 >SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 483 Score = 27.5 bits (58), Expect = 2.0 Identities = 11/33 (33%), Positives = 15/33 (45%) Frame = -3 Query: 398 FTSSCACKYSFFKFRCSSLMYSSCTSKNSNILW 300 F C S +F C ++ TSK N+LW Sbjct: 313 FPEPSFCSASKIRFNCDETYAATITSKYPNVLW 345 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 27.1 bits (57), Expect = 2.7 Identities = 20/69 (28%), Positives = 33/69 (47%) Frame = +1 Query: 196 EKDDQVTDSKGLAFAGPQSFDELESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEY 375 EK+ + K L A + E+E L + Q+ LE +E K E++ LK+E Sbjct: 593 EKEKKRDKKKQLKLAKEEERQRREAERLAEQ--AAQKALEAKRQEEARKKREEQRLKREQ 650 Query: 376 LHAQEEVKR 402 Q+E++R Sbjct: 651 EKKQQELER 659 >SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 27.1 bits (57), Expect = 2.7 Identities = 33/154 (21%), Positives = 68/154 (44%), Gaps = 3/154 (1%) Frame = +1 Query: 190 LPEKDDQVTDSKGLAFAGPQSFDELESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKK 369 L +K + +TD+ +S +E S+++ TK + + + +K E+ L + Sbjct: 317 LQDKLELLTDAYNREKRRARSLEERMSKEMLTKLGESNVDDGMAASRYDTVKREKERLSE 376 Query: 370 EYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASV 549 QE+ + IQSV + +D+ + I+ + +L+ + +LK Sbjct: 377 HLKSLQEQYEHIQSVYKNV-----LLDRESYIMRLGNKISENNELLN--ENRVLKEKLQT 429 Query: 550 ALHKHSNALVDVLPPEADSS---ISMLQADEKPD 642 L K + + + A++S +SM +ADE+ D Sbjct: 430 YLDKKESNVTSKIKSTAENSSKPLSMNEADERKD 463 >SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 347 Score = 26.2 bits (55), Expect = 4.7 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +1 Query: 364 KKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGI 465 K ++ A E+ + VPL +F + VDQN G+ Sbjct: 51 KSKHSEAVVEILKSAKVPLKNKEFSDLVDQNIGV 84 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 25.4 bits (53), Expect = 8.2 Identities = 10/21 (47%), Positives = 16/21 (76%) Frame = +1 Query: 394 VKRIQSVPLVIGQFLEAVDQN 456 +K+ +SVP + GQF+E V+ N Sbjct: 193 LKKSKSVPRLRGQFMEPVEHN 213 >SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 25.4 bits (53), Expect = 8.2 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +1 Query: 271 EDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQS 411 E+ + QR +E QEE IK+E+ ++E +EEV +S Sbjct: 333 EEAVKSLPETQRTTSRIETQEEEIKEEEMEGEEE--EEEEEVPNYES 377 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.4 bits (53), Expect = 8.2 Identities = 22/70 (31%), Positives = 30/70 (42%) Frame = +1 Query: 448 DQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQA 627 D+NT + SN + I ELL PS A+ +SN D+ P SS + A Sbjct: 14 DENTDALFKLHTSNRKSANMFGIKSELLNPSELSAVGSYSN---DICPNRQSSSST--AA 68 Query: 628 DEKPDVQYSD 657 D P S+ Sbjct: 69 DTSPSTNASN 78 >SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.4 bits (53), Expect = 8.2 Identities = 16/58 (27%), Positives = 27/58 (46%) Frame = +1 Query: 388 EEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHK 561 EE Q P +GQ+ + + Q I Y++RI+ ++LK S + +HK Sbjct: 212 EENLDFQESPHYLGQYKKLLSQAMDIF-----KPYFIRIIKQTTDQVLKDSKKMDVHK 264 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,610,557 Number of Sequences: 5004 Number of extensions: 49696 Number of successful extensions: 183 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 182 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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