BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0427 (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC15F9.03c |nxt2|nft2, ntf2, ntf2, nft2, SPAC1B9.01c|nuclear t... 83 5e-17 SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyc... 55 8e-09 SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1||... 27 2.4 SPCC1620.04c |mug55||Cdc20/Fizzy family WD repeat protein|Schizo... 27 3.2 SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 7.5 SPCC970.02 |||mannan endo-1,6-alpha-mannosidase|Schizosaccharomy... 25 7.5 SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 25 7.5 SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 25 9.9 >SPAC15F9.03c |nxt2|nft2, ntf2, ntf2, nft2, SPAC1B9.01c|nuclear transport factor Nxt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 123 Score = 82.6 bits (195), Expect = 5e-17 Identities = 48/120 (40%), Positives = 70/120 (58%), Gaps = 2/120 (1%) Frame = +2 Query: 59 YDAIGKGFVQQYYTLFDDPAQRANLVNMYNVETSFMTFEGVQLQGAVKIMEKLNSLTFQK 238 Y+A+ F Q YY FD + R+ L ++Y E S ++FEG QLQG I+EKL SL FQ+ Sbjct: 4 YNALATQFTQFYYQTFD--SDRSQLSSLYR-EESMLSFEGAQLQGTKAIVEKLVSLPFQR 60 Query: 239 ITRIVTAVDSQPM-FDGGVLINVLGRLKCDEDP-PHLYMQTFVLKPLGDSFYVQHDIFRL 412 + ++ +D+QP G V++ V G L DE+ Y Q F L ++YV +D+FRL Sbjct: 61 VQHRISTLDAQPTGTTGSVIVMVTGELLLDEEQMAQRYSQVFHLVNNNGNYYVLNDLFRL 120 >SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyces pombe|chr 2|||Manual Length = 434 Score = 55.2 bits (127), Expect = 8e-09 Identities = 38/126 (30%), Positives = 61/126 (48%), Gaps = 4/126 (3%) Frame = +2 Query: 62 DAIGKGFVQQYYTLFDDPAQRANLVNMYNVETSFM---TFEGVQL-QGAVKIMEKLNSLT 229 D IG FVQ+YYT + R + Y +++ + E + L G +I K+ L Sbjct: 16 DEIGWMFVQEYYTYLNKEPNRLHC--FYTKKSTLIHGDEGESISLCHGQQEIHNKILDLD 73 Query: 230 FQKITRIVTAVDSQPMFDGGVLINVLGRLKCDEDPPHLYMQTFVLKPLGDSFYVQHDIFR 409 FQ +++ VDS +GG++I VLG + + QTF L + ++V +DIFR Sbjct: 74 FQNCKVLISNVDSLASSNGGIVIQVLGEMSNKGKLSRKFAQTFFLAEQPNGYFVLNDIFR 133 Query: 410 LGIHDI 427 D+ Sbjct: 134 FLREDV 139 >SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 27.1 bits (57), Expect = 2.4 Identities = 12/31 (38%), Positives = 20/31 (64%), Gaps = 1/31 (3%) Frame = -2 Query: 666 FLCNENQILIL-DYPVRSLNRSLS*FHNRLV 577 F C +Q+L+ D V SLN+ ++ FH +L+ Sbjct: 245 FACRSSQLLVSEDNVVSSLNKLINDFHGKLI 275 >SPCC1620.04c |mug55||Cdc20/Fizzy family WD repeat protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 509 Score = 26.6 bits (56), Expect = 3.2 Identities = 17/61 (27%), Positives = 27/61 (44%) Frame = -2 Query: 420 WMPKRKMSC*T*NESPSGFNTNVCMYRCGGSSSHFNLPRTLIKTPPSNIGWESTAVTILV 241 W + K C + SP G N+++ +YR + F++P I GW TI+ Sbjct: 383 WSRRYKEFCYSLGYSPEGTNSSLIVYRWPQLTKVFDIPSAAID------GWGQDLRTIMA 436 Query: 240 I 238 I Sbjct: 437 I 437 >SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 2609 Score = 25.4 bits (53), Expect = 7.5 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = -2 Query: 366 FNTNVCMYRCGGSSSHFNLPRTLIKTPPSNIGWEST 259 F+ C+Y S F+ R L+ PPS I +ST Sbjct: 1267 FSILTCIYNRITSGQGFSYSRLLVYLPPSQIEKKST 1302 >SPCC970.02 |||mannan endo-1,6-alpha-mannosidase|Schizosaccharomyces pombe|chr 3|||Manual Length = 442 Score = 25.4 bits (53), Expect = 7.5 Identities = 15/54 (27%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = +2 Query: 77 GFVQQYYTLFDDPAQRANLVNMYNVETSFMTFE-GVQLQGAVKIMEKLNSLTFQ 235 GF+Q+ YT+FD + + N ++E + T+ G+ + GA + NS ++ Sbjct: 219 GFIQEDYTVFDGSSIKD---NCSSIEITQWTYNIGLYMAGAAYMYNYTNSTVWK 269 >SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 25.4 bits (53), Expect = 7.5 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -1 Query: 538 EKYNVYQMSRILIQQLYVISNFKLSISSCCVCP 440 E + +M +L +LY ISN LSI+ + P Sbjct: 410 EDFTYQRMKTVLDDELYTISNTNLSITDDLLPP 442 >SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +1 Query: 439 KDRHNTKKCLT*NLI 483 KD HNTKKCL LI Sbjct: 259 KDDHNTKKCLAQLLI 273 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,824,356 Number of Sequences: 5004 Number of extensions: 58670 Number of successful extensions: 121 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 116 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 118 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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