Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0416
(652 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 205 2e-53
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 145 2e-35
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 145 2e-35
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 143 1e-34
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 93 1e-19
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 52 4e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 52 4e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 4e-06
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 5e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 5e-04
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 0.88
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 2.7
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 6.2
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 8.2
At3g63180.1 68416.m07097 expressed protein 27 8.2
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 205 bits (501), Expect = 2e-53
Identities = 90/140 (64%), Positives = 114/140 (81%)
Frame = +2
Query: 26 LHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRG 205
LH+DAIHRGGGQ+IPT RR +YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RG
Sbjct: 694 LHSDAIHRGGGQVIPTARRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRG 753
Query: 206 HVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQ 385
HVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQAFPQCVFDHW+++ DP EP
Sbjct: 754 HVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPG 813
Query: 386 SKPYNVVQETRKRKGLKEGL 445
++ +V + RKRKGLKE +
Sbjct: 814 TQASVLVADIRKRKGLKEAM 833
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 145 bits (352), Expect = 2e-35
Identities = 76/147 (51%), Positives = 93/147 (63%), Gaps = 12/147 (8%)
Frame = +2
Query: 35 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 214
+ +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV
Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVT 872
Query: 215 EESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QS 388
+ GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++PGDP + Q
Sbjct: 873 SDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQL 932
Query: 389 KPY----------NVVQETRKRKGLKE 439
+P + +TR+RKG+ E
Sbjct: 933 RPLEPAPIQHLAREFMVKTRRRKGMSE 959
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 145 bits (352), Expect = 2e-35
Identities = 76/147 (51%), Positives = 93/147 (63%), Gaps = 12/147 (8%)
Frame = +2
Query: 35 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 214
+ +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV
Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVT 872
Query: 215 EESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QS 388
+ GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++PGDP + Q
Sbjct: 873 SDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQL 932
Query: 389 KPY----------NVVQETRKRKGLKE 439
+P + +TR+RKG+ E
Sbjct: 933 RPLEPAPIQHLAREFMVKTRRRKGMSE 959
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 143 bits (346), Expect = 1e-34
Identities = 75/147 (51%), Positives = 93/147 (63%), Gaps = 12/147 (8%)
Frame = +2
Query: 35 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 214
+ +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRG+V
Sbjct: 799 EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVT 858
Query: 215 EESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QS 388
+ GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++PGDP + Q
Sbjct: 859 SDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQL 918
Query: 389 KPY----------NVVQETRKRKGLKE 439
+P + +TR+RKG+ E
Sbjct: 919 RPLEPAPIQHLAREFMVKTRRRKGMSE 945
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 93.1 bits (221), Expect = 1e-19
Identities = 41/106 (38%), Positives = 61/106 (57%)
Frame = +2
Query: 56 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 235
GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + +E G
Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEG 912
Query: 236 TPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 373
+ +F V AY+PV+ESFGF +LR T G A V HW++L DP
Sbjct: 913 SSLFTVHAYVPVSESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 51.6 bits (118), Expect = 4e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = +2
Query: 95 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 274
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 275 ESFGFTADLRSNTGGQ 322
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 51.6 bits (118), Expect = 4e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = +2
Query: 95 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 274
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 275 ESFGFTADLRSNTGGQ 322
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 48.4 bits (110), Expect = 4e-06
Identities = 27/86 (31%), Positives = 44/86 (51%)
Frame = +2
Query: 107 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 286
A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741
Query: 287 FTADLRSNTGGQAFPQCVFDHWQVLP 364
+ + LR T G+A + V+P
Sbjct: 742 YVSTLRGMTKGRASYTMQLAKFDVVP 767
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 41.5 bits (93), Expect = 5e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = +2
Query: 116 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 295
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532
Query: 296 DLRSNTGGQAFPQCVFDHWQVLPGD 370
+ + + G A VFD + GD
Sbjct: 533 AILTASRGTAILNTVFDSYGPWAGD 557
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 41.5 bits (93), Expect = 5e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = +2
Query: 116 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 295
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531
Query: 296 DLRSNTGGQAFPQCVFDHWQVLPGD 370
+ + + G A VFD + GD
Sbjct: 532 AILTASRGTAILNTVFDSYGPWAGD 556
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 30.7 bits (66), Expect = 0.88
Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +2
Query: 125 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 301
EP + I P VG + + + RRG E + + +F+ K LP+ E F +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524
Query: 302 RSNTGGQA 325
+S T G A
Sbjct: 525 KSITSGYA 532
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 29.1 bits (62), Expect = 2.7
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -3
Query: 410 PVQRCRACSEVRTGLQG 360
PV RCR C+EV G QG
Sbjct: 62 PVHRCRLCAEVHIGKQG 78
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +2
Query: 338 VFDHWQVLPGDPCEPQSKPYNV 403
V D W PGD EP S+P NV
Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +1
Query: 199 SWSRFRRVPGGRY-TYVHCEGL 261
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 8.2
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +3
Query: 258 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 362
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,444,939
Number of Sequences: 28952
Number of extensions: 313921
Number of successful extensions: 786
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 781
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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