BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0414 (647 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g42010.1 68418.m05114 WD-40 repeat family protein contains Pf... 31 0.66 At5g23730.1 68418.m02782 transducin family protein / WD-40 repea... 29 3.5 At4g08470.1 68417.m01398 mitogen-activated protein kinase, putat... 29 3.5 At1g29940.1 68414.m03658 DNA-directed RNA polymerase family prot... 29 3.5 At3g48860.2 68416.m05337 expressed protein 28 4.7 At3g48860.1 68416.m05336 expressed protein 28 4.7 At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 tr... 28 4.7 At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 tr... 28 4.7 At1g01520.1 68414.m00068 myb family transcription factor similar... 28 4.7 >At5g42010.1 68418.m05114 WD-40 repeat family protein contains Pfam PF00400: WD domain, G-beta repeat; similar to WD-repeat protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo sapiens] Length = 709 Score = 31.1 bits (67), Expect = 0.66 Identities = 22/60 (36%), Positives = 29/60 (48%) Frame = +1 Query: 136 SRGSSHVSKKQASVVAPHSFRWSEENRGRNTSKQV*SLATEASAGHPSHAASTIESSTPR 315 S GSS S AS+ SFR S +NR +N Q+ L + S+ S A ES + R Sbjct: 116 SSGSSVSSFSTASLAEAFSFRGSFQNRAKNIDDQI-LLTRDYSSNSSSIAEGLSESDSSR 174 >At5g23730.1 68418.m02782 transducin family protein / WD-40 repeat family protein contains similarity to photomorphogenesis repressor protein; contains Pfam PF00400: WD domain, G-beta repeat (5 copies) Length = 243 Score = 28.7 bits (61), Expect = 3.5 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +1 Query: 322 SVRNRVFFYSRR*NKSIVINGSMPFCQNTG 411 S NRVF Y RR K + ++G P N+G Sbjct: 175 SENNRVFVYDRRWGKPVWVDGFEPVGMNSG 204 >At4g08470.1 68417.m01398 mitogen-activated protein kinase, putative similar to mitogen-activated protein kinase [Arabidopsis thaliana] gi|1255448|dbj|BAA09057; contains Pfam PF00069: Protein kinase domain Length = 560 Score = 28.7 bits (61), Expect = 3.5 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = -1 Query: 326 TERTRGVELSIVEAAWEGCPAEASVAKD*TCFEVLRPRFSS 204 TE TRG+ + + + A E CP + A + ++ P ++S Sbjct: 88 TEETRGMFVQLGDTAHEACPFATNEADSSSTVSIISPSYAS 128 >At1g29940.1 68414.m03658 DNA-directed RNA polymerase family protein similar to SP|P22138 DNA-directed RNA polymerase I 135 kDa polypeptide (EC 2.7.7.6) (RNA polymerase I subunit 2) {Saccharomyces cerevisiae}; contains Pfam profiles PF04563; RNA polymerase beta subunit, PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2 domain 3, PF00562: RNA polymerase Rpb2 domain 6 Length = 1114 Score = 28.7 bits (61), Expect = 3.5 Identities = 14/30 (46%), Positives = 17/30 (56%) Frame = +1 Query: 427 KNMPRCIHNYVRISLKTNREKTSASLDLRQ 516 KN PR I + LKT KT + LDL+Q Sbjct: 364 KNPPRSIGTSIETLLKTGALKTQSGLDLQQ 393 >At3g48860.2 68416.m05337 expressed protein Length = 577 Score = 28.3 bits (60), Expect = 4.7 Identities = 16/36 (44%), Positives = 22/36 (61%), Gaps = 1/36 (2%) Frame = +1 Query: 217 GRNTSKQV*SLATEASAGHPSHAA-STIESSTPRVL 321 GRN ++QV S ASAG PS +A ST + P ++ Sbjct: 134 GRNFAEQVPSSVRSASAGRPSMSARSTTPTPIPNLM 169 >At3g48860.1 68416.m05336 expressed protein Length = 494 Score = 28.3 bits (60), Expect = 4.7 Identities = 16/36 (44%), Positives = 22/36 (61%), Gaps = 1/36 (2%) Frame = +1 Query: 217 GRNTSKQV*SLATEASAGHPSHAA-STIESSTPRVL 321 GRN ++QV S ASAG PS +A ST + P ++ Sbjct: 134 GRNFAEQVPSSVRSASAGRPSMSARSTTPTPIPNLM 169 >At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 transmembrane domains; similar to DNA double-strand break repair rad50 ATPase. (Swiss-Prot:O33600) [Sulfolobus acidocaldarius] Length = 440 Score = 28.3 bits (60), Expect = 4.7 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +1 Query: 88 TIKTHTFGYKQQYAAKSRGSSHVSKKQASVVAPHSFRWSE 207 T+KTH + Q + K++ + H SK S V PH ++ E Sbjct: 272 TVKTHVEPHVQTLSTKAKEAYHASK---SAVTPHIVKFQE 308 >At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 transmembrane domains; similar to DNA double-strand break repair rad50 ATPase. (Swiss-Prot:O33600) [Sulfolobus acidocaldarius] Length = 440 Score = 28.3 bits (60), Expect = 4.7 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +1 Query: 88 TIKTHTFGYKQQYAAKSRGSSHVSKKQASVVAPHSFRWSE 207 T+KTH + Q + K++ + H SK S V PH ++ E Sbjct: 272 TVKTHVEPHVQTLSTKAKEAYHASK---SAVTPHIVKFQE 308 >At1g01520.1 68414.m00068 myb family transcription factor similar to myb-related protein GI:2505876 from [Arabidopsis thaliana] Length = 287 Score = 28.3 bits (60), Expect = 4.7 Identities = 13/31 (41%), Positives = 19/31 (61%), Gaps = 4/31 (12%) Frame = -2 Query: 523 PKTDAN----RVTPKFSLDSSSTIYVHSYVY 443 PK AN + PKF+L SS+ ++ H Y+Y Sbjct: 123 PKRKANHPYPQKAPKFTLSSSNALFQHDYLY 153 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,662,924 Number of Sequences: 28952 Number of extensions: 276170 Number of successful extensions: 725 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 702 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 725 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1344285648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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