Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0400
(723 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 235 4e-63
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 32 0.095
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 27 2.7
SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyce... 27 3.6
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 3.6
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 26 4.7
SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 25 8.3
SPAC926.02 |||conserved fungal protein|Schizosaccharomyces pombe... 25 8.3
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 235 bits (576), Expect = 4e-63
Identities = 109/172 (63%), Positives = 130/172 (75%)
Frame = +3
Query: 27 EYKKQFEDAGIWYEHRLIDDMVAYAMKSEGGFVWACKNYDGDVQSDSVAQGYGSLGLMTS 206
+YK++FE+ G+WY+HRLIDDMVA A+KS GGFVWACKNYDGDV SD VAQ YGSLGLMTS
Sbjct: 244 DYKQKFEELGLWYQHRLIDDMVAQAIKSNGGFVWACKNYDGDVMSDVVAQAYGSLGLMTS 303
Query: 207 VLICPDGKTVEAEAAHGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALK 386
VLI P+G+T E+EAAHGTV RH+ Y +GK+TSTN IASIFAWTRGL HR +LD N+ L
Sbjct: 304 VLIHPNGRTFESEAAHGTVQRHYMQYLKGKKTSTNSIASIFAWTRGLAHRGRLDGNERLV 363
Query: 387 NFAETLEKVCIETIESGIMTKDLAICIKGMNNVKRSDYYETFEFMDKLAENL 542
FA LE C+ +E GIMTKDL + K N Y +TFEF+D + L
Sbjct: 364 KFANALEHACVRCVEKGIMTKDLYLLSKSPNG-----YVDTFEFLDAVKSEL 410
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 31.9 bits (69), Expect = 0.095
Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = +3
Query: 51 AGIWYEHRLIDDMVAYAMKSEGGF-VWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDG 227
A I + +++D MV + F V N GD+ SD A GSLGL+ S +
Sbjct: 222 ASINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANV--GD 279
Query: 228 KTVEAEAAHGT 260
V +E HG+
Sbjct: 280 NFVMSEPVHGS 290
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 27.1 bits (57), Expect = 2.7
Identities = 17/73 (23%), Positives = 33/73 (45%)
Frame = +3
Query: 252 HGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIETIE 431
H T+ + Y+ G+ T+ + W L HRA + ++ K T+E+ +E I
Sbjct: 561 HLTLLNVYHAYKSGEGTAD------WCWNHFLSHRALISADNVRKQLRRTMERQEVELIS 614
Query: 432 SGIMTKDLAICIK 470
+ K+ + I+
Sbjct: 615 TPFDDKNYYVNIR 627
>SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 132
Score = 26.6 bits (56), Expect = 3.6
Identities = 18/57 (31%), Positives = 24/57 (42%), Gaps = 1/57 (1%)
Frame = +3
Query: 222 DGKTVEAEAAHGTVTR-HFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKN 389
DG T++ E HGT + H F +E NP A F W R + + KN
Sbjct: 56 DG-TLDPEMTHGTKPKEHGEFDTDDEEEEENPDAE-FRWGYSARRRIRKQREEYFKN 110
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 26.6 bits (56), Expect = 3.6
Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = +3
Query: 60 WYEH-RLIDDMVAYAMKSEGGFVWACKNYDGDVQSD---SVAQGYGSLGLMTSVLICPDG 227
W H DD+ + G V+ KNYDGDV++D + + G+ + + P+G
Sbjct: 794 WLGHPSYFDDLTSLDPNLYEGLVFL-KNYDGDVENDMALNFTVVHEEFGVRNVIDLIPNG 852
Query: 228 KTV 236
+
Sbjct: 853 SNI 855
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 26.2 bits (55), Expect = 4.7
Identities = 15/46 (32%), Positives = 20/46 (43%)
Frame = -1
Query: 183 HSPEQHYQIVRLHHNFCRPRQIHPLIS*HKPPYHQLACVHTRYQHP 46
H P +H + H F RP + HKP H+ C H R+ P
Sbjct: 278 HKPWKHEEHCH-HGKFPRPIPHNGTKPDHKPWKHEEHCHHGRFPRP 322
Score = 25.8 bits (54), Expect = 6.3
Identities = 14/46 (30%), Positives = 20/46 (43%)
Frame = -1
Query: 183 HSPEQHYQIVRLHHNFCRPRQIHPLIS*HKPPYHQLACVHTRYQHP 46
H P +H + H F RP + HKP H+ C H ++ P
Sbjct: 361 HKPWKHEEHCH-HGKFLRPVPHNVTKPDHKPWKHEEHCHHGKFPRP 405
>SPAC22A12.11 |dak1||dihydroxyacetone kinase
Dak1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 8.3
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 102 MKSEGGFVWACKNYDGDV 155
++SE G + CKNY GD+
Sbjct: 93 VESEAGTLVICKNYTGDI 110
>SPAC926.02 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 443
Score = 25.4 bits (53), Expect = 8.3
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 3/31 (9%)
Frame = +3
Query: 366 DNNDALKNFAETLEKVCIETIESG---IMTK 449
D DA +FAETL C+ T ES IMT+
Sbjct: 301 DKADAYIDFAETLLDSCVSTEESASIEIMTR 331
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,819,972
Number of Sequences: 5004
Number of extensions: 55973
Number of successful extensions: 133
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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