Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0400
(723 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 266 1e-71
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 265 2e-71
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 252 1e-67
At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NA... 38 0.009
At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NA... 36 0.027
At5g48550.1 68418.m06003 F-box family protein-related similar to... 29 3.1
At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase, chloro... 27 9.5
At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase, chloro... 27 9.5
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 266 bits (651), Expect = 1e-71
Identities = 123/175 (70%), Positives = 144/175 (82%)
Frame = +3
Query: 30 YKKQFEDAGIWYEHRLIDDMVAYAMKSEGGFVWACKNYDGDVQSDSVAQGYGSLGLMTSV 209
+K +++ AGIWYEHRLIDDMVAYA+KSEGG+VWACKNYDGDVQSD +AQG+GSLGLMTSV
Sbjct: 235 WKSKYDAAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSV 294
Query: 210 LICPDGKTVEAEAAHGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKN 389
L+CPDGKT+EAEAAHGTVTRHFR +Q+G ETSTN IASIFAWTRGL HRAKLD+N L +
Sbjct: 295 LVCPDGKTIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNAKLLD 354
Query: 390 FAETLEKVCIETIESGIMTKDLAICIKGMNNVKRSDYYETFEFMDKLAENLKVAL 554
F E LE C+ T+ESG MTKDLA+ I G + + R Y T EF+D +A LK L
Sbjct: 355 FTEKLEAACVGTVESGKMTKDLALIIHG-SKLSRDTYLNTEEFIDAVAAELKERL 408
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 265 bits (650), Expect = 2e-71
Identities = 122/172 (70%), Positives = 144/172 (83%)
Frame = +3
Query: 30 YKKQFEDAGIWYEHRLIDDMVAYAMKSEGGFVWACKNYDGDVQSDSVAQGYGSLGLMTSV 209
++ ++E AGIWYEHRLIDDMVAYAMKSEGG+VWACKNYDGDVQSD +AQGYGSLG+MTSV
Sbjct: 236 WRSKYEAAGIWYEHRLIDDMVAYAMKSEGGYVWACKNYDGDVQSDFLAQGYGSLGMMTSV 295
Query: 210 LICPDGKTVEAEAAHGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKN 389
L+CPDGKT+EAEAAHGTVTRH+R +Q+G ETSTN IASIFAW+RGL HRAKLD+N AL +
Sbjct: 296 LVCPDGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDSNAALLS 355
Query: 390 FAETLEKVCIETIESGIMTKDLAICIKGMNNVKRSDYYETFEFMDKLAENLK 545
+ E LE C+ T+ESG MTKDLA+ I G V+R Y T EF+D +A LK
Sbjct: 356 YTEKLEAACMGTVESGKMTKDLALLIHGA-KVRRDQYVNTEEFIDAVAWELK 406
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 252 bits (618), Expect = 1e-67
Identities = 117/172 (68%), Positives = 141/172 (81%)
Frame = +3
Query: 30 YKKQFEDAGIWYEHRLIDDMVAYAMKSEGGFVWACKNYDGDVQSDSVAQGYGSLGLMTSV 209
+K++FE+ IWYEHRLIDDMVAYA+KSEGG+VWACKNYDGDVQSD +AQG+GSLGLMTSV
Sbjct: 306 WKQKFEEHSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSV 365
Query: 210 LICPDGKTVEAEAAHGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKN 389
L+ DGKT+E+EAAHGTVTRHFR +Q+G+ETSTN IASIFAWTRGL HRAKLD N+ L +
Sbjct: 366 LLSADGKTLESEAAHGTVTRHFRLHQKGQETSTNSIASIFAWTRGLEHRAKLDKNEKLMD 425
Query: 390 FAETLEKVCIETIESGIMTKDLAICIKGMNNVKRSDYYETFEFMDKLAENLK 545
F + LE C+ T+E+G MTKDLA+ I G V R + T EF+D +A LK
Sbjct: 426 FVKKLESSCVNTVETGKMTKDLALLIHG-PKVSRDLFLNTEEFIDAVASKLK 476
>At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) GB:CAA65502 GI:3021506
[Nicotiana tabacum]
Length = 374
Score = 37.5 bits (83), Expect = 0.009
Identities = 27/69 (39%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
Frame = +3
Query: 57 IWYEHRLIDDMVAYAMKSEGGF-VWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKT 233
I+YE +ID+ +K+ F V N GD+ SD A G LGL S+ I DG
Sbjct: 237 IYYEKVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSMNIGEDGIA 296
Query: 234 VEAEAAHGT 260
+ AEA HG+
Sbjct: 297 L-AEAVHGS 304
>At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
GI:3021506
Length = 374
Score = 35.9 bits (79), Expect = 0.027
Identities = 27/69 (39%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = +3
Query: 57 IWYEHRLIDDMVAYAMKSEGGF-VWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKT 233
I YE +ID+ +K+ F V N GD+ SD A G LGL S I DG
Sbjct: 237 ITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVA 296
Query: 234 VEAEAAHGT 260
+ AEA HG+
Sbjct: 297 L-AEAVHGS 304
>At5g48550.1 68418.m06003 F-box family protein-related similar to
unknown protein (gb AAF19735.1); contains TIGRFAM
TIGR01640 : F-box protein interaction domain
Length = 427
Score = 29.1 bits (62), Expect = 3.1
Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 1/74 (1%)
Frame = +3
Query: 288 QGKETSTNPIASIFAWTRGLLHRAKLDNNDALKNFAET-LEKVCIETIESGIMTKDLAIC 464
+G + STN + + AW GLL R NF T +E + E G++++D I
Sbjct: 276 RGHDWSTNRLHVVGAWKEGLLLRIWDSTVCIYLNFKTTRMEYIGFENEIQGLISRDFIIS 335
Query: 465 IKGMNNVKRSDYYE 506
++ YY+
Sbjct: 336 YTPNYLLRLKTYYD 349
>At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}
Length = 409
Score = 27.5 bits (58), Expect = 9.5
Identities = 28/130 (21%), Positives = 52/130 (40%), Gaps = 1/130 (0%)
Frame = +3
Query: 69 HRLIDDMVAYAMKSEGGF-VWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKTVEAE 245
H +D+ ++ F N GD+ SD + GS+G++ S + G + E
Sbjct: 264 HMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGL-FE 322
Query: 246 AAHGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIET 425
HG+ + NP+A+I + +L + L A A+ +E ++
Sbjct: 323 PIHGSAP------DIAGQDKANPLATIL--SAAMLLKYGLGEEKA----AKRIEDAVVDA 370
Query: 426 IESGIMTKDL 455
+ G T D+
Sbjct: 371 LNKGFRTGDI 380
>At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}; EST gb|F14478 comes from this gene
Length = 404
Score = 27.5 bits (58), Expect = 9.5
Identities = 28/130 (21%), Positives = 52/130 (40%), Gaps = 1/130 (0%)
Frame = +3
Query: 69 HRLIDDMVAYAMKSEGGF-VWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKTVEAE 245
H +D+ ++ F N GD+ SD + GS+G++ S + G + E
Sbjct: 261 HMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGL-FE 319
Query: 246 AAHGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIET 425
HG+ + NP+A+I + +L + L A A+ +E ++
Sbjct: 320 PIHGSAP------DIAGQDKANPLATIL--SAAMLLKYGLGEEKA----AKMIEDAVVDA 367
Query: 426 IESGIMTKDL 455
+ G T D+
Sbjct: 368 LNKGFRTGDI 377
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,572,885
Number of Sequences: 28952
Number of extensions: 292302
Number of successful extensions: 688
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 676
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 688
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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