BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0387 (684 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g75560.1 68414.m08781 zinc knuckle (CCHC-type) family protein... 40 0.002 At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein... 35 0.044 At5g36240.1 68418.m04372 zinc knuckle (CCHC-type) family protein... 33 0.23 At5g20750.1 68418.m02466 Ulp1 protease family protein contains P... 31 0.71 At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein... 31 0.71 At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein... 31 0.71 At2g37340.3 68415.m04580 splicing factor RSZ33 (RSZ33) nearly id... 31 0.71 At2g37340.2 68415.m04579 splicing factor RSZ33 (RSZ33) nearly id... 31 0.71 At2g37340.1 68415.m04581 splicing factor RSZ33 (RSZ33) nearly id... 31 0.71 At3g02670.1 68416.m00258 proline-rich family protein contains pr... 27 1.7 At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative... 29 2.9 At4g03970.1 68417.m00561 Ulp1 protease family protein contains P... 29 3.8 At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing ... 28 5.0 At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model... 28 5.0 At4g12500.1 68417.m01975 protease inhibitor/seed storage/lipid t... 28 5.0 At1g32190.1 68414.m03959 expressed protein 28 5.0 At1g13530.1 68414.m01586 expressed protein ; expression support... 28 5.0 At5g32613.1 68418.m03881 zinc knuckle (CCHC-type) family protein... 28 6.6 At2g24860.1 68415.m02973 chaperone protein dnaJ-related similar ... 28 6.6 At5g51730.1 68418.m06415 expressed protein 27 8.8 At5g40900.1 68418.m04966 expressed protein similar to GI:2827651... 27 8.8 At4g12490.1 68417.m01974 protease inhibitor/seed storage/lipid t... 27 8.8 At3g59390.2 68416.m06624 expressed protein protein CG15643 - Dro... 27 8.8 At3g59390.1 68416.m06623 expressed protein protein CG15643 - Dro... 27 8.8 At2g18120.1 68415.m02109 lateral root primordium (LRP) protein-r... 27 8.8 >At1g75560.1 68414.m08781 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 257 Score = 39.5 bits (88), Expect = 0.002 Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%) Frame = -2 Query: 509 CFNCCRFGHTKVQCRSKPRCFKCGQ-GHTGDTCNVEEDCVSC 387 C NC GH +C ++ RC+ C + GH C+ E C SC Sbjct: 76 CNNCGLPGHIAAECTAESRCWNCREPGHVASNCSNEGICHSC 117 Score = 35.1 bits (77), Expect = 0.044 Identities = 16/42 (38%), Positives = 18/42 (42%), Gaps = 1/42 (2%) Frame = -2 Query: 509 CFNCCRFGHTKVQCRSKPRCFKCG-QGHTGDTCNVEEDCVSC 387 C NC R GH C + C CG GH C E C +C Sbjct: 57 CNNCKRPGHFARDCSNVSVCNNCGLPGHIAAECTAESRCWNC 98 Score = 32.7 bits (71), Expect = 0.23 Identities = 19/58 (32%), Positives = 25/58 (43%), Gaps = 1/58 (1%) Frame = -2 Query: 509 CFNCCRFGHTKVQCRSKPRCFKC-GQGHTGDTCNVEEDCVSCCLCSGLHFASSKKCPE 339 C NC + GH C + C C GH C + C + C SG H A + CP+ Sbjct: 140 CNNCFKQGHLAADCTNDKACKNCRTSGHIARDCRNDPVC-NICSISG-HVA--RHCPK 193 Score = 31.5 bits (68), Expect = 0.54 Identities = 12/35 (34%), Positives = 18/35 (51%), Gaps = 1/35 (2%) Frame = -2 Query: 512 QCFNCCRFGHTKVQCRSKPRCFKCGQ-GHTGDTCN 411 +C+NC GH C ++ C CG+ GH C+ Sbjct: 94 RCWNCREPGHVASNCSNEGICHSCGKSGHRARDCS 128 >At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 551 Score = 35.1 bits (77), Expect = 0.044 Identities = 18/49 (36%), Positives = 22/49 (44%), Gaps = 6/49 (12%) Frame = -2 Query: 515 IQCFNCCRFGHTKVQC-----RSKPRCFKCGQ-GHTGDTCNVEEDCVSC 387 + C++C GHT C R KP CF CG H C+ DC C Sbjct: 166 VSCYSCGEQGHTSFNCPTPTKRRKP-CFICGSLEHGAKQCSKGHDCYIC 213 Score = 28.7 bits (61), Expect = 3.8 Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 9/43 (20%) Frame = -2 Query: 515 IQCFNCCRFGHTKVQCRSKP--------RCFKCGQ-GHTGDTC 414 +QC+ C FGH C +P C++CGQ GH+G C Sbjct: 261 VQCYICKSFGHL---CCVEPGNSLSWAVSCYRCGQLGHSGLAC 300 >At5g36240.1 68418.m04372 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 254 Score = 32.7 bits (71), Expect = 0.23 Identities = 15/43 (34%), Positives = 21/43 (48%), Gaps = 7/43 (16%) Frame = -2 Query: 518 TIQCFNCCRFGHTKVQC------RSKPRCFKCG-QGHTGDTCN 411 T+ C+ C + GHT + C P CF CG +GH C+ Sbjct: 76 TVSCYRCGQLGHTGLACGRHYDDSVSPSCFICGREGHFEHQCH 118 >At5g20750.1 68418.m02466 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 944 Score = 31.1 bits (67), Expect = 0.71 Identities = 20/71 (28%), Positives = 32/71 (45%) Frame = -2 Query: 278 ASKLHPPISKSFADIVSSLPTKSVPGGNTVLPGSPSSRTISYKKTVFTKPRTPPQHSKGF 99 + +LH S FAD+ + +KS + V P ++S K + F + PP S+ Sbjct: 489 SQQLHLLRSNLFADMEKLVESKSASFKSGVRPSRKVKTSLSKKPSPFVSKKPPPSDSRKR 548 Query: 98 DHVAHESLVRD 66 V +SL D Sbjct: 549 QRVEVQSLSPD 559 >At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein contains Pfam domain PF00098: Zinc knuckle Length = 284 Score = 31.1 bits (67), Expect = 0.71 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%) Frame = -2 Query: 512 QCFNCCRFGHTKVQCRS---KPRCFKCGQ-GHTGDTC 414 +CFNC GH C + K +C++CG+ GH C Sbjct: 100 RCFNCGVDGHWARDCTAGDWKNKCYRCGERGHIERNC 136 >At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein contains Pfam domain PF00098: Zinc knuckle Length = 243 Score = 31.1 bits (67), Expect = 0.71 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%) Frame = -2 Query: 512 QCFNCCRFGHTKVQCRS---KPRCFKCGQ-GHTGDTC 414 +CFNC GH C + K +C++CG+ GH C Sbjct: 59 RCFNCGVDGHWARDCTAGDWKNKCYRCGERGHIERNC 95 >At2g37340.3 68415.m04580 splicing factor RSZ33 (RSZ33) nearly identical to splicing factor RSZ33 [Arabidopsis thaliana] GI:9843663; contains Pfam profiles PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain), PF00098: Zinc knuckle Length = 249 Score = 31.1 bits (67), Expect = 0.71 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%) Frame = -2 Query: 512 QCFNCCRFGHTKVQCRS---KPRCFKCGQ-GHTGDTC 414 +CFNC GH C + K +C++CG+ GH C Sbjct: 59 RCFNCGVDGHWARDCTAGDWKNKCYRCGERGHIERNC 95 >At2g37340.2 68415.m04579 splicing factor RSZ33 (RSZ33) nearly identical to splicing factor RSZ33 [Arabidopsis thaliana] GI:9843663; contains Pfam profiles PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain), PF00098: Zinc knuckle Length = 260 Score = 31.1 bits (67), Expect = 0.71 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%) Frame = -2 Query: 512 QCFNCCRFGHTKVQCRS---KPRCFKCGQ-GHTGDTC 414 +CFNC GH C + K +C++CG+ GH C Sbjct: 70 RCFNCGVDGHWARDCTAGDWKNKCYRCGERGHIERNC 106 >At2g37340.1 68415.m04581 splicing factor RSZ33 (RSZ33) nearly identical to splicing factor RSZ33 [Arabidopsis thaliana] GI:9843663; contains Pfam profiles PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain), PF00098: Zinc knuckle Length = 290 Score = 31.1 bits (67), Expect = 0.71 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%) Frame = -2 Query: 512 QCFNCCRFGHTKVQCRS---KPRCFKCGQ-GHTGDTC 414 +CFNC GH C + K +C++CG+ GH C Sbjct: 100 RCFNCGVDGHWARDCTAGDWKNKCYRCGERGHIERNC 136 >At3g02670.1 68416.m00258 proline-rich family protein contains proline rich extensin domains, INTERPRO:IPR002965 Length = 217 Score = 26.6 bits (56), Expect(2) = 1.7 Identities = 11/19 (57%), Positives = 12/19 (63%) Frame = -2 Query: 221 PTKSVPGGNTVLPGSPSSR 165 P S PGGN +PGSP R Sbjct: 80 PFPSSPGGNPGIPGSPGFR 98 Score = 21.8 bits (44), Expect(2) = 1.7 Identities = 8/19 (42%), Positives = 12/19 (63%) Frame = -2 Query: 257 ISKSFADIVSSLPTKSVPG 201 +S F+ + SS PT +PG Sbjct: 53 VSDPFSSLQSSPPTSPIPG 71 >At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative strong similarity to gi:4467359 Length = 1116 Score = 29.1 bits (62), Expect = 2.9 Identities = 12/47 (25%), Positives = 25/47 (53%) Frame = -3 Query: 310 QWQRIAYPILKRQNYIPPFLNHLLILYPLSQLNLFLVEIQYCLVHHP 170 +W ++Y + + + +L + + PLS + +L +I Y +VH P Sbjct: 48 EWIAVSYLYKHQHSGVRDYLCNRMYTLPLSGIESYLFQICYLMVHKP 94 >At4g03970.1 68417.m00561 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to At5g28170, At1g35110, At1g44880, At3g42530, At4g19320, At5g36020, At3g43010, At2g10350 Length = 1043 Score = 28.7 bits (61), Expect = 3.8 Identities = 15/55 (27%), Positives = 26/55 (47%) Frame = -2 Query: 263 PPISKSFADIVSSLPTKSVPGGNTVLPGSPSSRTISYKKTVFTKPRTPPQHSKGF 99 P +S++ +V+ L +KS G +LP S ++ T+ P T S G+ Sbjct: 804 PTLSEAEVFLVAELLSKSKTGSYELLPSMSKSEFALFRNTLSAAPNTEHLTSSGY 858 >At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing protein low similarity to transcriptional repressor Not4-Np [Homo sapiens] GI:6856207; contains INTERPRO:IPR000504 RNA-binding region RNP-1 (RNA recognition motif) Length = 944 Score = 28.3 bits (60), Expect = 5.0 Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 2/68 (2%) Frame = -2 Query: 365 FASSKKCPEYVRQTDIKVTMAENSLSYIEASKLHPPISKSFADIVSSLPTKSVPGGN-TV 189 F + Y R ++T A N L + + L PP+ +D S+ P VP N T Sbjct: 183 FTKDEIISAYTRSRVQQITGATNILQHHSGNMLPPPLDAYCSDSSSAKPIIKVPSTNATS 242 Query: 188 LPG-SPSS 168 +P SP S Sbjct: 243 VPRYSPPS 250 >At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model reflects the alignment of a full-length cDNA sequence gi:18496702. There are multiple frame shifts in the gene model resulting in a truncated protein. The alternate model includes modifications in exons 14, 17 and 29 to compensate for frame shifts and maximize the protein length. It is not based on EST data. adapter protein SPIKE1 [Arabidopsis thaliana] GI:18496703 Length = 1757 Score = 28.3 bits (60), Expect = 5.0 Identities = 16/49 (32%), Positives = 27/49 (55%), Gaps = 6/49 (12%) Frame = -2 Query: 650 KVMINNTPTWKPSQTVVFTF---DGQV---LPKRVFMCYNSMPVKLYIY 522 ++ ++ TW PS ++FTF D Q P+ V + Y S+P+ YI+ Sbjct: 531 EIKVSLPATWTPSHHLLFTFFHVDLQTKLEAPRPVVVGYASLPLSTYIH 579 >At4g12500.1 68417.m01975 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to pEARLI 1 (Accession No. L43080): an Arabidopsis member of a conserved gene family (PGF95-099), Plant Physiol. 109 (4), 1497 (1995); contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 Length = 177 Score = 28.3 bits (60), Expect = 5.0 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = -2 Query: 227 SLPTKSVPGGNTVLPGSPSSRTISYKKTVFTKPRTP 120 S+P+ SVP + P P+ S T T PRTP Sbjct: 53 SIPSPSVPTPSVPTPSVPTPSVPSPNPTPVTPPRTP 88 >At1g32190.1 68414.m03959 expressed protein Length = 422 Score = 28.3 bits (60), Expect = 5.0 Identities = 16/44 (36%), Positives = 18/44 (40%) Frame = -2 Query: 503 NCCRFGHTKVQCRSKPRCFKCGQGHTGDTCNVEEDCVSCCLCSG 372 +CC C KP+C KC C E C S C CSG Sbjct: 351 SCCCPTFKCSSCFGKPKCPKCSCWKCLK-CPDTECCRSSCCCSG 393 >At1g13530.1 68414.m01586 expressed protein ; expression supported by MPSS Length = 385 Score = 28.3 bits (60), Expect = 5.0 Identities = 12/20 (60%), Positives = 13/20 (65%), Gaps = 2/20 (10%) Frame = -2 Query: 431 HTGDTC--NVEEDCVSCCLC 378 H G+ C EED VSCCLC Sbjct: 105 HAGEACVSAKEEDKVSCCLC 124 >At5g32613.1 68418.m03881 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 457 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = -2 Query: 530 YIYPTIQCFNCCRFGHTKVQCRSKP 456 Y P +C NC R+GH +C SKP Sbjct: 308 YPRPPPKCLNCGRYGHLLSRC-SKP 331 >At2g24860.1 68415.m02973 chaperone protein dnaJ-related similar to Tsi1-interacting protein TSIP1 (GI:4337001) [Nicotiana tabacum] Length = 144 Score = 27.9 bits (59), Expect = 6.6 Identities = 20/59 (33%), Positives = 25/59 (42%), Gaps = 2/59 (3%) Frame = -2 Query: 515 IQCFNCCRFGHTKVQCRSKPRCFKCGQGHTGDT--CNVEEDCVSCCLCSGLHFASSKKC 345 + C +C GH V+C+ C G GD C V SC +CSG AS C Sbjct: 74 VGCSSCNSKGH--VECKW---CAGTGFFILGDNMLCQVPSRNTSCVICSGQGSASCSDC 127 >At5g51730.1 68418.m06415 expressed protein Length = 412 Score = 27.5 bits (58), Expect = 8.8 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = +1 Query: 214 LVGREDTISANDLEMGGCNFD 276 L G ED + N +MGGCN + Sbjct: 246 LQGTEDALKLNGSDMGGCNLE 266 >At5g40900.1 68418.m04966 expressed protein similar to GI:2827651, GI:7527728, GI:4406788,GI:6063544, GI:10764853 from [Arabidopsis thaliana]; expression supported by MPSS Length = 248 Score = 27.5 bits (58), Expect = 8.8 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 3/49 (6%) Frame = -3 Query: 253 LNHLLILYPLSQLNLFLVEIQYCLVHHPHV---QYLIKKQSSLNPELLL 116 LNHL+++ Q ++YCL HPH +Y KK SL P+ L+ Sbjct: 30 LNHLIVVALDDQA------LRYCLRAHPHCYLHRYSRKKSESLKPDGLV 72 >At4g12490.1 68417.m01974 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to pEARLI 1 (Accession No. L43080): an Arabidopsis member of a conserved gene family (PGF95-099), Plant Physiol. 109 (4), 1497 (1995); contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 Length = 182 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = -2 Query: 224 LPTKSVPGGNTVLPGSPSSRTISYKKTVFTKPRTP 120 +PT SVP + P PS S T PRTP Sbjct: 59 VPTPSVPSPSVPTPSVPSPSVPSPNPTPVIPPRTP 93 >At3g59390.2 68416.m06624 expressed protein protein CG15643 - Drosophila melanogaster, EMBL:AE003499 Length = 273 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/31 (45%), Positives = 14/31 (45%), Gaps = 1/31 (3%) Frame = -2 Query: 470 CRSKPRCFKC-GQGHTGDTCNVEEDCVSCCL 381 C K F C G CN E CVSCCL Sbjct: 70 CPWKGERFSCHGCNILSQCCNSYEFCVSCCL 100 >At3g59390.1 68416.m06623 expressed protein protein CG15643 - Drosophila melanogaster, EMBL:AE003499 Length = 273 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/31 (45%), Positives = 14/31 (45%), Gaps = 1/31 (3%) Frame = -2 Query: 470 CRSKPRCFKC-GQGHTGDTCNVEEDCVSCCL 381 C K F C G CN E CVSCCL Sbjct: 70 CPWKGERFSCHGCNILSQCCNSYEFCVSCCL 100 >At2g18120.1 68415.m02109 lateral root primordium (LRP) protein-related similar to lateral root primordium 1 (LRP1) [Arabidopsis thaliana] GI:882341; contains Pfam profile PF05142: Domain of unknown function (DUF702) Length = 222 Score = 27.5 bits (58), Expect = 8.8 Identities = 19/65 (29%), Positives = 27/65 (41%), Gaps = 1/65 (1%) Frame = -2 Query: 452 CFKCG-QGHTGDTCNVEEDCVSCCLCSGLHFASSKKCPEYVRQTDIKVTMAENSLSYIEA 276 C +CG Q G T C +CC +GLH CP +VR T I + ++ Sbjct: 72 CQECGNQAKKGCT---HGRCRTCCKSNGLH------CPTHVRSTWIPIAKRRERQQQLQT 122 Query: 275 SKLHP 261 +P Sbjct: 123 PTSNP 127 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,262,511 Number of Sequences: 28952 Number of extensions: 364610 Number of successful extensions: 1078 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 1008 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1074 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1447936096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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