Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0383
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 301 3e-82
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 179 1e-45
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 179 1e-45
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 175 2e-44
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 119 2e-27
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 69 3e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 60 1e-09
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 60 1e-09
At2g47500.1 68415.m05929 kinesin motor protein-related 30 1.2
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 30 1.6
At1g67560.1 68414.m07697 lipoxygenase family protein similar to ... 29 2.9
At1g70890.1 68414.m08179 major latex protein-related / MLP-relat... 29 3.8
At1g70160.1 68414.m08073 expressed protein similar to hypothetic... 29 3.8
At5g17300.1 68418.m02026 myb family transcription factor similar... 28 5.0
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 5.0
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 6.6
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 28 6.6
At5g38500.1 68418.m04654 hypothetical protein contains Pfam prof... 27 8.8
At3g47480.1 68416.m05163 calcium-binding EF hand family protein ... 27 8.8
At3g42790.1 68416.m04474 PHD finger family protein contains PHD-... 27 8.8
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 301 bits (739), Expect = 3e-82
Identities = 147/225 (65%), Positives = 177/225 (78%), Gaps = 3/225 (1%)
Frame = +1
Query: 19 EAIEDVPSGNICGLVGVDQFLVKTGTITTFK--NAHNMKVMKFSVSPVVRVAVEPKNPAD 192
E +EDVP GN +VG+DQF+ K T+T K +AH ++ MKFSVSPVVRVAV+ K +D
Sbjct: 441 ETVEDVPCGNTVAMVGLDQFITKNATLTNEKEVDAHPIRAMKFSVSPVVRVAVQCKVASD 500
Query: 193 LPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDH-ACIPIKKSDP 369
LPKLVEGLKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL++D I KSDP
Sbjct: 501 LPKLVEGLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDP 560
Query: 370 VVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYL 549
VVS+RETV + S + +SKSPNKHNRL+M+A+PM +GL E ID+GR+ PRDD K R++ L
Sbjct: 561 VVSFRETVCDRSTRTVMSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKIL 620
Query: 550 TEKYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQYLNEIKDSVV 684
E++ +D A+KIW FGPE TGPN++VD KGVQYLNEIKDSVV
Sbjct: 621 AEEFGWDKDLAKKIWAFGPETTGPNMVVDMCKGVQYLNEIKDSVV 665
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 179 bits (436), Expect = 1e-45
Identities = 89/227 (39%), Positives = 142/227 (62%), Gaps = 7/227 (3%)
Frame = +1
Query: 25 IEDVPSGNICGLVGVDQFLVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADL 195
+ P G+ + GVD ++KT T+ ++ + + ++F+ PVV+ A EP NP++L
Sbjct: 555 VSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSEL 614
Query: 196 PKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 375
PK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV
Sbjct: 615 PKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVV 674
Query: 376 SYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTE 555
S+ ETV E S C +++PNK N++ M A+P+ GL EDI+ G V+ + K +
Sbjct: 675 SFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRT 734
Query: 556 KYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVV 684
KY++D+ AR IW FGP+ GPNIL+D + + + +KDS+V
Sbjct: 735 KYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIV 781
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 179 bits (436), Expect = 1e-45
Identities = 89/227 (39%), Positives = 142/227 (62%), Gaps = 7/227 (3%)
Frame = +1
Query: 25 IEDVPSGNICGLVGVDQFLVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADL 195
+ P G+ + GVD ++KT T+ ++ + + ++F+ PVV+ A EP NP++L
Sbjct: 555 VSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSEL 614
Query: 196 PKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 375
PK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV
Sbjct: 615 PKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVV 674
Query: 376 SYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTE 555
S+ ETV E S C +++PNK N++ M A+P+ GL EDI+ G V+ + K +
Sbjct: 675 SFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRT 734
Query: 556 KYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVV 684
KY++D+ AR IW FGP+ GPNIL+D + + + +KDS+V
Sbjct: 735 KYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIV 781
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 175 bits (426), Expect = 2e-44
Identities = 89/227 (39%), Positives = 140/227 (61%), Gaps = 7/227 (3%)
Frame = +1
Query: 25 IEDVPSGNICGLVGVDQFLVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADL 195
+ P G+ + GVD ++KT T+ ++ + + +KF+ PVV+ A EP NP++L
Sbjct: 541 VSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALKFNTLPVVKTATEPLNPSEL 600
Query: 196 PKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 375
PK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV
Sbjct: 601 PKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVV 660
Query: 376 SYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTE 555
S+ ETV E S C +++PNK N+L M A+P+ GL EDI+ G V+ + +
Sbjct: 661 SFCETVVESSSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRT 720
Query: 556 KYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVV 684
KY++D+ AR IW FGP+ G NIL+D + + + +KDS+V
Sbjct: 721 KYDWDLLAARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIV 767
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 119 bits (287), Expect = 2e-27
Identities = 52/130 (40%), Positives = 88/130 (67%)
Frame = +1
Query: 25 IEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKL 204
+ +V +GN+ + G+ ++ K+ T+++ +N + M+F VSP +RVA+EP +PAD+ L
Sbjct: 495 VTEVKAGNVVAIRGLGPYISKSATLSSTRNCWPLASMEFQVSPTLRVAIEPSDPADMSAL 554
Query: 205 VEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYR 384
++GL+ L ++DP V+ GEH++A AGE+HLE C+KDL+E A + ++ S P+VSYR
Sbjct: 555 MKGLRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLKERFAKVNLEVSPPLVSYR 614
Query: 385 ETVAEESDQL 414
ET+ + L
Sbjct: 615 ETIEGDGSNL 624
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 68.9 bits (161), Expect = 3e-12
Identities = 43/129 (33%), Positives = 74/129 (57%), Gaps = 2/129 (1%)
Frame = +1
Query: 19 EAIEDVPSGNICGLVGVDQFLVKTG-TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADL 195
E ++ +G+I L G+ + TG T++ +N ++ M F PV++VA+EPK AD+
Sbjct: 451 EDVKVALTGDIIALAGLKDTI--TGETLSDPENPVVLERMDFP-DPVIKVAIEPKTKADI 507
Query: 196 PKLVEGLKRLAKSDPMVQ-CINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPV 372
K+ GL +LA+ DP +EE + ++ G GELHLEI + L+ + + P
Sbjct: 508 DKMATGLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRLKREFK-VEANVGAPQ 566
Query: 373 VSYRETVAE 399
V+YRE++++
Sbjct: 567 VNYRESISK 575
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 60.5 bits (140), Expect = 1e-09
Identities = 44/165 (26%), Positives = 81/165 (49%), Gaps = 5/165 (3%)
Frame = +1
Query: 19 EAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLP 198
E I++ +G I + G++ T T + M M PV+ +AV+P +
Sbjct: 420 EDIQEAHAGQIVAVFGIE--CASGDTFTDGSVKYTMTSMNVP-EPVMSLAVQPVSKDSGG 476
Query: 199 KLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 375
+ + L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V
Sbjct: 477 QFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRV 535
Query: 376 SYRETVAE--ESDQLCLSKS--PNKHNRLFMKAQPMPDGLPEDID 498
++RET+ + E D L +S ++ R+ +P+P G E +
Sbjct: 536 NFRETITQRAEFDYLHKKQSGGAGQYGRVTGYVEPLPPGSKEKFE 580
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 60.5 bits (140), Expect = 1e-09
Identities = 44/165 (26%), Positives = 81/165 (49%), Gaps = 5/165 (3%)
Frame = +1
Query: 19 EAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLP 198
E I++ +G I + G++ T T + M M PV+ +AV+P +
Sbjct: 420 EDIQEAHAGQIVAVFGIE--CASGDTFTDGSVKYTMTSMNVP-EPVMSLAVQPVSKDSGG 476
Query: 199 KLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 375
+ + L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V
Sbjct: 477 QFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRV 535
Query: 376 SYRETVAE--ESDQLCLSKS--PNKHNRLFMKAQPMPDGLPEDID 498
++RET+ + E D L +S ++ R+ +P+P G E +
Sbjct: 536 NFRETITQRAEFDYLHKKQSGGAGQYGRVTGYVEPLPPGSKEKFE 580
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 109 KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGL 216
+N N S+S +VR + K P D+PKL+E L
Sbjct: 218 ENDSNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESL 253
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +1
Query: 400 ESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 495
E + LCL PN+H + + A+ +P LPE +
Sbjct: 44 EKENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At1g67560.1 68414.m07697 lipoxygenase family protein similar to
13-lipoxygenase GB:CAA65269 [Solanum tuberosum],
gi:1654140 [Lycopersicon esculentum]
Length = 917
Score = 29.1 bits (62), Expect = 2.9
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +1
Query: 421 SKSPNKHNRLFMKAQPM-----PDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 573
SK+ N R+ ++QP PDG+ E ++ V+ R D K + Y+YDV
Sbjct: 204 SKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 259
>At1g70890.1 68414.m08179 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:294062] ; contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 158
Score = 28.7 bits (61), Expect = 3.8
Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
Frame = -1
Query: 471 HGLSLHEQTVVLVGRL*ETELVRFLSYG-LTVRHDRVRLLDWNASMVLLKILKTDLKMEF 295
HG LHE VG + + +++ G LTV +++ +D +++ K+L+ DL E+
Sbjct: 44 HGCELHEGDWGKVGSI---VIWKYVHDGKLTVGKNKIEAVDPEKNLITFKVLEGDLMNEY 100
Query: 294 SS 289
S
Sbjct: 101 KS 102
>At1g70160.1 68414.m08073 expressed protein similar to hypothetical
protein GI:4455225 from [Arabidopsis thaliana]
Length = 523
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/81 (25%), Positives = 30/81 (37%)
Frame = +1
Query: 256 NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPN 435
NE+ E IV + E+ LKD + D + T A E + L K
Sbjct: 272 NEKGEEIIVVIPWDEWWELTLKDNSNPQVALLPLHPDIRAKFNNTAAWEYARSMLGKPYG 331
Query: 436 KHNRLFMKAQPMPDGLPEDID 498
HN +F + D P +D
Sbjct: 332 YHNMIFSWIDTLGDNYPPPLD 352
>At5g17300.1 68418.m02026 myb family transcription factor similar to
CCA1 [Arabidopsis thaliana] GI:4090569; contains Pfam
profile PF00249: Myb-like DNA-binding domain
Length = 387
Score = 28.3 bits (60), Expect = 5.0
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -2
Query: 404 DSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPAT 279
D+ + S T GS+ L + + S +RSL +S S PA T
Sbjct: 154 DTQSPTSVLSTVGSEALCSLDSSSPNRSLSPVSSASPPAALT 195
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +1
Query: 253 INEESGEHIVAGAGELHLEICLKDLEEDH 339
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1
Query: 286 GAGELHLEICLKDLEEDHACIPIKK 360
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -1
Query: 390 GLTVRHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 274
G TV+ DRV W +++ +++TD +EF N+
Sbjct: 348 GGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At5g38500.1 68418.m04654 hypothetical protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 411
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +1
Query: 328 EEDHACIPIKKSDPVVSYRETVAEESD--QLCLSKSPNKHNRLFMKAQPMPDGL 483
EE + +K + S ++TV+EE D + LS P K +K Q P+G+
Sbjct: 25 EEQRRRLLAEKREDSKSQKKTVSEEDDSEKRFLSHVPRKKRSSLVKRQQKPNGV 78
>At3g47480.1 68416.m05163 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 183
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/45 (31%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
Frame = +1
Query: 151 PVVRVAVEPKNPADLPKLVEGLKRLAK-SDPMVQCINEESGEHIV 282
P+++ V P N AD+ VE +K + S+ + +C+ EE E+++
Sbjct: 63 PIIKKVVVP-NRADIKTSVEEVKAIIDDSEALYECLIEEGEEYLL 106
>At3g42790.1 68416.m04474 PHD finger family protein contains
PHD-finger domain, INTERPRO:IPR001965
Length = 250
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/62 (25%), Positives = 27/62 (43%)
Frame = +1
Query: 304 LEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGL 483
+E KD + I + V + + E + LCL PN+ + + A+ +P L
Sbjct: 13 VEEVFKDFKGRRTAIVKALTTDVQEFYQQCDPEKENLCLYGLPNEEWEVNLPAEEVPPEL 72
Query: 484 PE 489
PE
Sbjct: 73 PE 74
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.136 0.405
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,964,965
Number of Sequences: 28952
Number of extensions: 352805
Number of successful extensions: 1133
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1093
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1124
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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