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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0376
         (634 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC576.06c |||tyrosine-tRNA ligase|Schizosaccharomyces pombe|ch...    31   0.18 
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc...    29   0.74 
SPBC336.13c |||mitochondrial inner membrane peptidase complex ca...    28   0.97 
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb...    28   1.3  
SPCC962.05 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||...    26   5.2  
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large...    25   6.9  
SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc...    25   6.9  
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ...    25   9.1  
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo...    25   9.1  
SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos...    25   9.1  

>SPCC576.06c |||tyrosine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 445

 Score = 30.7 bits (66), Expect = 0.18
 Identities = 18/57 (31%), Positives = 29/57 (50%)
 Frame = +1

Query: 220 INSKLNENDSWKPVSAGSVKSLKPIPGGHVYGESEYTFHSSSDINGQKTDKSGGHKI 390
           +N ++  +D W  ++AG+    +  P  +VY  +     SSS   GQK  KS G+ I
Sbjct: 201 VNLQIGGSDQWGNITAGTDLVRRTHPNANVYALTTPLLTSSS---GQKLGKSAGNAI 254


>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 920

 Score = 28.7 bits (61), Expect = 0.74
 Identities = 20/82 (24%), Positives = 36/82 (43%)
 Frame = +1

Query: 166 DELFDAASSGDWEKVHELINSKLNENDSWKPVSAGSVKSLKPIPGGHVYGESEYTFHSSS 345
           DEL+  +S     ++  +IN  L + +  + +SA S+   +       YG    T+H   
Sbjct: 356 DELYLQSSRNSEPEISTIINDSLQQENMDEDISATSIPKSQA-----AYGHGSVTYHEVP 410

Query: 346 DINGQKTDKSGGHKIINDDGKV 411
             N   T  S G+ I +  G++
Sbjct: 411 RYN--LTSASVGYSISSQRGRI 430


>SPBC336.13c |||mitochondrial inner membrane peptidase complex
           catalytic subunit 2|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 180

 Score = 28.3 bits (60), Expect = 0.97
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = +1

Query: 280 SLKPIPGGHVYGESEYTFHS 339
           SL P+P GHV+ E +  FHS
Sbjct: 111 SLVPVPEGHVWVEGDEQFHS 130


>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 330

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 16/43 (37%), Positives = 25/43 (58%)
 Frame = +1

Query: 160 IGDELFDAASSGDWEKVHELINSKLNENDSWKPVSAGSVKSLK 288
           IGD  F      + +  H +IN+KL++++  K V   SVKS+K
Sbjct: 281 IGDVCFSMPRKLNKDGAHRIINAKLSKDEDAKLVE--SVKSIK 321


>SPCC962.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 536

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +1

Query: 199 WEKVHELINSKLNENDSWKP 258
           W +V EL+NS+L   + W P
Sbjct: 288 WGRVQELLNSQLQPAEIWLP 307


>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
            subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1405

 Score = 25.4 bits (53), Expect = 6.9
 Identities = 11/29 (37%), Positives = 16/29 (55%)
 Frame = +2

Query: 296  PEAMCTVNPSTHSIHRQTSTDRRPTKAEV 382
            P    TVN +   IH  TS+D + ++ EV
Sbjct: 1175 PSQQVTVNNTLQQIHVHTSSDGKSSETEV 1203


>SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor
           Mcl1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 25.4 bits (53), Expect = 6.9
 Identities = 13/56 (23%), Positives = 28/56 (50%)
 Frame = +1

Query: 238 ENDSWKPVSAGSVKSLKPIPGGHVYGESEYTFHSSSDINGQKTDKSGGHKIINDDG 405
           END  KP+++   K+        ++G  +    + +D++G   ++   ++ IN DG
Sbjct: 309 ENDPTKPLTSSKSKNRTSKELDDLFGSDDEQSQNVNDLDGNSANEE--NEFINHDG 362


>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 695

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = +1

Query: 148 PSFPIGDELFDAASSGDWEKVHELINSKLNENDSWK 255
           P +   +  FD  +    E++ EL+NS L+E +  K
Sbjct: 544 PKYSSQETAFDTGAPISEEQIEELLNSGLDEQEGEK 579


>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 9/29 (31%), Positives = 20/29 (68%)
 Frame = -3

Query: 353 LMSDDEWNVYSDSPYTWPPGIGFKLLTLP 267
           ++S ++W++Y+++     P I FKL+ +P
Sbjct: 450 VLSPEQWHLYANTVGFPIPSIEFKLVDIP 478


>SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 732

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 20/79 (25%), Positives = 35/79 (44%), Gaps = 3/79 (3%)
 Frame = +2

Query: 194 EIGKKFTN*LTVNSTRTIVGNQCQRAVSKA*SQYPEAMCTVNPSTHS---IHRQTSTDRR 364
           E G +F    T+ S  T +G    R VS + ++  +     +   HS   I   +   R 
Sbjct: 182 ECGARFPQ--TLKSPLTPIGGDSARTVSASTARTSDKFFPRHTRAHSDFWIPATSKPSRH 239

Query: 365 PTKAEVTKLSTMTEKSTSS 421
            + + +  L+T+T+ S SS
Sbjct: 240 ASHSSIGDLTTITQSSISS 258


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,421,795
Number of Sequences: 5004
Number of extensions: 46110
Number of successful extensions: 157
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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