BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0376 (634 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.06c |||tyrosine-tRNA ligase|Schizosaccharomyces pombe|ch... 31 0.18 SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 29 0.74 SPBC336.13c |||mitochondrial inner membrane peptidase complex ca... 28 0.97 SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 28 1.3 SPCC962.05 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 26 5.2 SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 6.9 SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc... 25 6.9 SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ... 25 9.1 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 25 9.1 SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 25 9.1 >SPCC576.06c |||tyrosine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 445 Score = 30.7 bits (66), Expect = 0.18 Identities = 18/57 (31%), Positives = 29/57 (50%) Frame = +1 Query: 220 INSKLNENDSWKPVSAGSVKSLKPIPGGHVYGESEYTFHSSSDINGQKTDKSGGHKI 390 +N ++ +D W ++AG+ + P +VY + SSS GQK KS G+ I Sbjct: 201 VNLQIGGSDQWGNITAGTDLVRRTHPNANVYALTTPLLTSSS---GQKLGKSAGNAI 254 >SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces pombe|chr 3|||Manual Length = 920 Score = 28.7 bits (61), Expect = 0.74 Identities = 20/82 (24%), Positives = 36/82 (43%) Frame = +1 Query: 166 DELFDAASSGDWEKVHELINSKLNENDSWKPVSAGSVKSLKPIPGGHVYGESEYTFHSSS 345 DEL+ +S ++ +IN L + + + +SA S+ + YG T+H Sbjct: 356 DELYLQSSRNSEPEISTIINDSLQQENMDEDISATSIPKSQA-----AYGHGSVTYHEVP 410 Query: 346 DINGQKTDKSGGHKIINDDGKV 411 N T S G+ I + G++ Sbjct: 411 RYN--LTSASVGYSISSQRGRI 430 >SPBC336.13c |||mitochondrial inner membrane peptidase complex catalytic subunit 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 180 Score = 28.3 bits (60), Expect = 0.97 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +1 Query: 280 SLKPIPGGHVYGESEYTFHS 339 SL P+P GHV+ E + FHS Sbjct: 111 SLVPVPEGHVWVEGDEQFHS 130 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 27.9 bits (59), Expect = 1.3 Identities = 16/43 (37%), Positives = 25/43 (58%) Frame = +1 Query: 160 IGDELFDAASSGDWEKVHELINSKLNENDSWKPVSAGSVKSLK 288 IGD F + + H +IN+KL++++ K V SVKS+K Sbjct: 281 IGDVCFSMPRKLNKDGAHRIINAKLSKDEDAKLVE--SVKSIK 321 >SPCC962.05 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 536 Score = 25.8 bits (54), Expect = 5.2 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +1 Query: 199 WEKVHELINSKLNENDSWKP 258 W +V EL+NS+L + W P Sbjct: 288 WGRVQELLNSQLQPAEIWLP 307 >SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1405 Score = 25.4 bits (53), Expect = 6.9 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +2 Query: 296 PEAMCTVNPSTHSIHRQTSTDRRPTKAEV 382 P TVN + IH TS+D + ++ EV Sbjct: 1175 PSQQVTVNNTLQQIHVHTSSDGKSSETEV 1203 >SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mcl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/56 (23%), Positives = 28/56 (50%) Frame = +1 Query: 238 ENDSWKPVSAGSVKSLKPIPGGHVYGESEYTFHSSSDINGQKTDKSGGHKIINDDG 405 END KP+++ K+ ++G + + +D++G ++ ++ IN DG Sbjct: 309 ENDPTKPLTSSKSKNRTSKELDDLFGSDDEQSQNVNDLDGNSANEE--NEFINHDG 362 >SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = +1 Query: 148 PSFPIGDELFDAASSGDWEKVHELINSKLNENDSWK 255 P + + FD + E++ EL+NS L+E + K Sbjct: 544 PKYSSQETAFDTGAPISEEQIEELLNSGLDEQEGEK 579 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 25.0 bits (52), Expect = 9.1 Identities = 9/29 (31%), Positives = 20/29 (68%) Frame = -3 Query: 353 LMSDDEWNVYSDSPYTWPPGIGFKLLTLP 267 ++S ++W++Y+++ P I FKL+ +P Sbjct: 450 VLSPEQWHLYANTVGFPIPSIEFKLVDIP 478 >SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.0 bits (52), Expect = 9.1 Identities = 20/79 (25%), Positives = 35/79 (44%), Gaps = 3/79 (3%) Frame = +2 Query: 194 EIGKKFTN*LTVNSTRTIVGNQCQRAVSKA*SQYPEAMCTVNPSTHS---IHRQTSTDRR 364 E G +F T+ S T +G R VS + ++ + + HS I + R Sbjct: 182 ECGARFPQ--TLKSPLTPIGGDSARTVSASTARTSDKFFPRHTRAHSDFWIPATSKPSRH 239 Query: 365 PTKAEVTKLSTMTEKSTSS 421 + + + L+T+T+ S SS Sbjct: 240 ASHSSIGDLTTITQSSISS 258 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,421,795 Number of Sequences: 5004 Number of extensions: 46110 Number of successful extensions: 157 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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