BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0370 (585 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 73 1e-13 At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 71 8e-13 At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 68 4e-12 At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 66 2e-11 At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 57 1e-08 At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 56 2e-08 At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 56 2e-08 At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 55 3e-08 At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 38 0.004 At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 37 0.011 At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 31 0.75 At2g28880.1 68415.m03510 para-aminobenzoate (PABA) synthase fami... 31 0.75 At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) fa... 30 0.99 At1g61300.1 68414.m06909 disease resistance protein (NBS-LRR cla... 27 9.2 At1g22100.1 68414.m02763 hypothetical protein 27 9.2 >At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5 [Arabidopsis thaliana] GI:4240122; strong similarity to Cytochrome B5 SP:P49098 from [Nicotiana tabacum] Length = 140 Score = 72.9 bits (171), Expect = 1e-13 Identities = 36/84 (42%), Positives = 52/84 (61%) Frame = +2 Query: 8 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 187 +T +EV+ + K I+ID VYDV FL+DHPGG EV++ + G DA++ F +VGHS Sbjct: 8 FTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSST 67 Query: 188 AIEWRNTFKVGEIVDEEKLEVKCK 259 A + + VG+I D + VK K Sbjct: 68 AKAMLDEYYVGDI-DTATVPVKAK 90 >At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cytochrome B5 SP:P49098 from [Nicotiana tabacum] Length = 134 Score = 70.5 bits (165), Expect = 8e-13 Identities = 33/73 (45%), Positives = 47/73 (64%) Frame = +2 Query: 8 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 187 +T +EV+ + I+I+ VY+V FLEDHPGG +VL+ + G DA++ F +VGHSE Sbjct: 8 FTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSES 67 Query: 188 AIEWRNTFKVGEI 226 A E + VGEI Sbjct: 68 AREMMEQYYVGEI 80 >At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana] Length = 134 Score = 68.1 bits (159), Expect = 4e-12 Identities = 30/69 (43%), Positives = 44/69 (63%) Frame = +2 Query: 20 EVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEIAIEW 199 EV+ + K +II VYDV F++DHPGG EVL+ + G DA+ F +VGHS+ A + Sbjct: 12 EVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDM 71 Query: 200 RNTFKVGEI 226 + + +GEI Sbjct: 72 MDKYFIGEI 80 >At1g26340.1 68414.m03212 cytochrome b5, putative similar to cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis thaliana] Length = 135 Score = 66.1 bits (154), Expect = 2e-11 Identities = 30/79 (37%), Positives = 49/79 (62%) Frame = +2 Query: 8 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 187 Y+ E A + ++ID VYDV++++++HPGG +VL+ AG DA++ F + GHS+ Sbjct: 8 YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67 Query: 188 AIEWRNTFKVGEIVDEEKL 244 A E + +GE+ DE L Sbjct: 68 ARELMEKYFIGEL-DESSL 85 >At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1) (NR1){Arabidopsis thaliana} Length = 917 Score = 56.8 bits (131), Expect = 1e-08 Identities = 26/74 (35%), Positives = 45/74 (60%) Frame = +2 Query: 8 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 187 Y+ +EV + II+ +YD FL+DHPGGT+ ++ NAG+D +E F + HS+ Sbjct: 548 YSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-HSDK 606 Query: 188 AIEWRNTFKVGEIV 229 A + +++GE++ Sbjct: 607 AKKLLEDYRIGELI 620 >At2g46650.1 68415.m05820 cytochrome b5, putative similar to cytochome b5 GI:2695711 from [Olea europaea] Length = 132 Score = 56.0 bits (129), Expect = 2e-08 Identities = 34/107 (31%), Positives = 60/107 (56%), Gaps = 6/107 (5%) Frame = +2 Query: 56 IIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEIAIEWRNTFKVGEI--- 226 I+I VYD++ F+++HPGG VL+ G DAS F +V HS+ A E + +G++ Sbjct: 21 ILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAKELMKKYCIGDVDQS 80 Query: 227 ---VDEEKLEVKCKQPSAAESAEPLTLGGLLAVYGPPVAMAVLAYLL 358 V ++ + K+ +AAE+ + + G L +Y P+ + +A+ L Sbjct: 81 TVPVTQQYIPPWEKESTAAETTKEES-GKKLLIYLIPLLILGVAFAL 126 >At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein contains InterPro accession IPR001199: Cytochrome b5 Length = 121 Score = 55.6 bits (128), Expect = 2e-08 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +2 Query: 8 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 187 Y+++EVA + III + VYD+ +++E+HPGG + ++D+AG D+++ F H+ Sbjct: 49 YSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTDGFFGPQHATR 107 Query: 188 AIEWRNTFKVGEI 226 + F +GE+ Sbjct: 108 VFDMIEDFYIGEL 120 >At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1) (NR2) {Arabidopsis thaliana} Length = 917 Score = 55.2 bits (127), Expect = 3e-08 Identities = 25/74 (33%), Positives = 45/74 (60%) Frame = +2 Query: 8 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 187 Y+ +EV ++ II+ +YD FL DHPGG++ ++ NAG+D +E F + HS+ Sbjct: 545 YSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAI-HSDK 603 Query: 188 AIEWRNTFKVGEIV 229 A + +++GE++ Sbjct: 604 AKKMLEDYRIGELI 617 >At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative similar to delta-8 sphingolipid desaturase GI:3819708 from [Brassica napus] Length = 449 Score = 38.3 bits (85), Expect = 0.004 Identities = 18/54 (33%), Positives = 31/54 (57%), Gaps = 1/54 (1%) Frame = +2 Query: 5 KYTRAEVAARDNNKQNL-IIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECF 163 +Y +E + N +L I I VYDV+ +++ HPGG +++ AG D ++ F Sbjct: 8 RYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61 >At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) identical to delta-8 sphingolipid desaturase GI:3819710 from [Arabidopsis thaliana]; contains Pfam profile PF00487: Fatty acid desaturase; contains Pfam profile PF00173: Heme/Steroid binding domain Length = 449 Score = 36.7 bits (81), Expect = 0.011 Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = +2 Query: 5 KYTRAEVAARDNNKQNL-IIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECF 163 KY E + N +L I I VY+V+ +++ HPGG V+++ G D ++ F Sbjct: 8 KYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61 >At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803 Length = 211 Score = 30.7 bits (66), Expect = 0.75 Identities = 10/37 (27%), Positives = 23/37 (62%) Frame = +2 Query: 59 IIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHE 169 ++ VY+++ ++ HPGG ++L+ G D + F++ Sbjct: 146 VLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNK 182 >At2g28880.1 68415.m03510 para-aminobenzoate (PABA) synthase family protein similar to PABA synthase from Streptomyces griseus [SP|P32483], Streptomyces pristinaespiralis [gi:1575336]; contains Pfam profiles PF00425: chorismate binding enzyme, PF00117: glutamine amidotransferase class-I, PF04715: Anthranilate synthase component I, N terminal region Length = 919 Score = 30.7 bits (66), Expect = 0.75 Identities = 19/47 (40%), Positives = 27/47 (57%), Gaps = 1/47 (2%) Frame = -1 Query: 258 LHFTSNFSSSTIS-PTLNVLRHSMAISLWPTSWKHSEASEPALSTRT 121 ++F+ +SS +S P+ NVLR S+A L+ WK S S P S T Sbjct: 3 MNFSFCSTSSELSYPSENVLRFSVASRLFSPKWKKSFISLPCRSKTT 49 >At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 4706 Score = 30.3 bits (65), Expect = 0.99 Identities = 16/36 (44%), Positives = 23/36 (63%), Gaps = 2/36 (5%) Frame = -1 Query: 201 RHSMAISL--WPTSWKHSEASEPALSTRTSVPPGWS 100 R+S A+ L WP +W +S P LST+ +PPG+S Sbjct: 4015 RYSEAVLLRDWPLAW----SSVPILSTQRFIPPGFS 4046 >At1g61300.1 68414.m06909 disease resistance protein (NBS-LRR class), putative domain signature NBS-LRR exists, suggestive of a disease resistance protein. Length = 762 Score = 27.1 bits (57), Expect = 9.2 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +2 Query: 167 EVGHSEIAIEWRNTFKVGEIVDEEKLEVKCKQPSAA 274 EV ++A R+ GE+ D + ++VKC +P A Sbjct: 168 EVNKCKVAFTTRDQKVCGEMGDHKPMQVKCLEPEDA 203 >At1g22100.1 68414.m02763 hypothetical protein Length = 457 Score = 27.1 bits (57), Expect = 9.2 Identities = 10/32 (31%), Positives = 21/32 (65%) Frame = +2 Query: 14 RAEVAARDNNKQNLIIIDNVVYDVAAFLEDHP 109 RA+VA+ D N+ +++++D++ +ED P Sbjct: 145 RADVASVDTNRSSVLLMDDLTLFAHGHVEDKP 176 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,620,080 Number of Sequences: 28952 Number of extensions: 193505 Number of successful extensions: 579 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 572 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 578 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1151426952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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