BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0355 (445 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4B4.06 |vps25||ESCRT II complex subunit Vps25|Schizosaccharo... 62 5e-11 SPBC1198.06c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 26 3.0 SPCC970.02 |||mannan endo-1,6-alpha-mannosidase|Schizosaccharomy... 26 3.0 SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 25 5.2 SPCC290.02 |rpc34||DNA-directed RNA polymerase III complex subun... 25 5.2 SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 25 6.9 SPAC607.04 |||inositol polyphosphate kinase |Schizosaccharomyces... 25 6.9 SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 24 9.1 SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|c... 24 9.1 >SPBC4B4.06 |vps25||ESCRT II complex subunit Vps25|Schizosaccharomyces pombe|chr 2|||Manual Length = 175 Score = 61.7 bits (143), Expect = 5e-11 Identities = 32/73 (43%), Positives = 43/73 (58%) Frame = +2 Query: 29 KNVWEVYWHSLDEWGNMIYNWACNNGFNNSVCTLFELREGDNTADQEFHNLDMNVLVKAL 208 K+V+ VYW S+ EWGNMI W + G S+CT +EL+E +E LD +L K L Sbjct: 93 KDVFWVYWRSISEWGNMILKWLSDMGREGSICTFYELQE----QYKEVDCLDEVLLHKVL 148 Query: 209 KSLEAKGRCELME 247 + L KG ELM+ Sbjct: 149 ELLMKKGNIELMK 161 >SPBC1198.06c |||mannan endo-1,6-alpha-mannosidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 25.8 bits (54), Expect = 3.0 Identities = 10/32 (31%), Positives = 20/32 (62%) Frame = +2 Query: 65 EWGNMIYNWACNNGFNNSVCTLFELREGDNTA 160 +W N I++W + GF N+ T + + +G +T+ Sbjct: 201 DWANKIWDWTTDVGFVNT--TTYAVYDGADTS 230 >SPCC970.02 |||mannan endo-1,6-alpha-mannosidase|Schizosaccharomyces pombe|chr 3|||Manual Length = 442 Score = 25.8 bits (54), Expect = 3.0 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = +2 Query: 65 EWGNMIYNWACNNGFNNSVCTLFE 136 EW N+ Y+W+ GF T+F+ Sbjct: 206 EWANVAYDWSQRIGFIQEDYTVFD 229 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 25.0 bits (52), Expect = 5.2 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = -3 Query: 98 YMPSCKSYFPTHPASANIPPT 36 YMPS SY PT P+ + P+ Sbjct: 1571 YMPSSPSYSPTSPSYSPTSPS 1591 >SPCC290.02 |rpc34||DNA-directed RNA polymerase III complex subunit Rpc34|Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 25.0 bits (52), Expect = 5.2 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +2 Query: 176 NLDMNVLVKALKSLEAKGRCELMEFDDNQGVKFF*MNILRTRTELQHG 319 NL ++V+ + LKSLE+K + ++ N K + + L TEL G Sbjct: 112 NLHVSVVDRCLKSLESKNLVKSIKSVKNPTRKIYMLYDLVPSTELTGG 159 >SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1375 Score = 24.6 bits (51), Expect = 6.9 Identities = 11/42 (26%), Positives = 22/42 (52%) Frame = -3 Query: 347 KSPSYDLCWIHAAVPFSSLICSFKRTLHPGCHQIP*VRIDLW 222 KSP D+CW V + ++ + + + ++IP R++ W Sbjct: 413 KSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIP--RVNCW 452 >SPAC607.04 |||inositol polyphosphate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 268 Score = 24.6 bits (51), Expect = 6.9 Identities = 14/40 (35%), Positives = 21/40 (52%), Gaps = 1/40 (2%) Frame = -2 Query: 312 CSSVLVLNMFI*KNFTPWL-SSNSMSSHRPLASSDFNALT 196 C+ + L +I KNF W+ SS + P+A S +LT Sbjct: 40 CNDNITLYNWIPKNFGEWMPSSRDIEGINPIAESVAFSLT 79 >SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 24.2 bits (50), Expect = 9.1 Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 2/33 (6%) Frame = +2 Query: 23 KSKNVWEVYWHSLDEWGNMIYNW--ACNNGFNN 115 K+KNV Y+ LDEW ++ N+ A +G NN Sbjct: 304 KNKNV---YYSRLDEWLHITLNYGLALRSGSNN 333 >SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 801 Score = 24.2 bits (50), Expect = 9.1 Identities = 10/40 (25%), Positives = 17/40 (42%) Frame = +2 Query: 20 DKSKNVWEVYWHSLDEWGNMIYNWACNNGFNNSVCTLFEL 139 D +K+ + W + D +Y + C N V FE+ Sbjct: 95 DVNKDSITIMWDNPDFQDGTLYEFTCENCLQEGVAYTFEM 134 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,925,060 Number of Sequences: 5004 Number of extensions: 38439 Number of successful extensions: 125 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 101 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 125 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 162176800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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