BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NRPG0346 (688 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schi... 29 0.83 SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 29 0.83 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 28 1.1 SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Sc... 28 1.5 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 28 1.5 SPBC902.02c |ctf18|chl12|DNA replication factor C complex subuni... 27 1.9 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 27 1.9 SPBP18G5.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 3.4 SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ... 27 3.4 SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 27 3.4 SPCC16A11.02 |utp13|SPCC63.16|U3 snoRNP-associated protein Utp13... 27 3.4 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 27 3.4 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 27 3.4 SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyc... 26 4.4 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.9 SPAC3A11.09 |sod22||plasma membrane alkali metal cation/H+ antip... 26 5.9 SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 26 5.9 SPCC126.05c |mrpl17||mitochondrial ribosomal protein subunit L17... 25 7.8 SPAPB8E5.09 |||AAA family ATPase Rvb1 |Schizosaccharomyces pombe... 25 7.8 SPAC1002.10c |sgt1||SGT1 family transcriptional regulator Sgt1|S... 25 7.8 SPAC589.08c |dam1||DASH complex subunit Dam1|Schizosaccharomyces... 25 7.8 >SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 28.7 bits (61), Expect = 0.83 Identities = 23/110 (20%), Positives = 52/110 (47%), Gaps = 1/110 (0%) Frame = +2 Query: 149 ASTAASDSEPTSLPSLPQEDKVAEETGDRIGNYSNEVNHVPIGEKNGFRRRSTYPPLSAS 328 + +++ D++ +S S + + +E++ + +E G + S+ S+ Sbjct: 119 SESSSEDNDSSSSSSDSESESSSEDSDSSSSSSDSESESSSEGSDSSSSSSSSESESSSE 178 Query: 329 ILSNFEENFESLIDNLT-DADSLTSSTETEVFKSVGDSDSACSDESGYSE 475 + + +S ++ + D+DS +SS+++E S SDS+ S S SE Sbjct: 179 DNDSSSSSSDSESESSSEDSDSSSSSSDSESESSSEGSDSSSSSSSSESE 228 Score = 26.2 bits (55), Expect = 4.4 Identities = 24/118 (20%), Positives = 55/118 (46%), Gaps = 2/118 (1%) Frame = +2 Query: 149 ASTAASDSEPTSLPSLPQEDKVAEETGDRIGNYSNEVNHVPIGEKNGFRRRSTYPPLSAS 328 + +++ D++ +S S + + +E++ + +E G + S+ S+ Sbjct: 173 SESSSEDNDSSSSSSDSESESSSEDSDSSSSSSDSESESSSEGSDSSSSSSSSESESSSE 232 Query: 329 ILSNFEENFESLIDNLT-DADSLTSSTETEVFKSVGDSDSAC-SDESGYSEFLDGKES 496 + + +S ++ + D+DS +SS+++E S DSDS+ S +S D +S Sbjct: 233 DNDSSSSSSDSESESSSEDSDSSSSSSDSESESSSKDSDSSSNSSDSEDDSSSDSSDS 290 >SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual Length = 304 Score = 28.7 bits (61), Expect = 0.83 Identities = 21/86 (24%), Positives = 36/86 (41%) Frame = +2 Query: 239 GNYSNEVNHVPIGEKNGFRRRSTYPPLSASILSNFEENFESLIDNLTDADSLTSSTETEV 418 G + V G++N ++R Y P + S L FE E L+ D+D+ + + + Sbjct: 142 GRLKTRQDFVLKGQQNNWKR---YNPTTKSYLEEFESQKEKLVYLSADSDNTITELDEDK 198 Query: 419 FKSVGDSDSACSDESGYSEFLDGKES 496 +G A D++ Y K S Sbjct: 199 IYIIG----AIVDKNRYKNLCQNKAS 220 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = -1 Query: 571 CELIICTDFHEIATHDLNHNSVPNNAFLSIQELGVA 464 CE ++CT H + HD + NAFL+I L V+ Sbjct: 1047 CEPLLCTLCHLLVIHDSRTVGLVVNAFLAITPLLVS 1082 >SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 27.9 bits (59), Expect = 1.5 Identities = 15/45 (33%), Positives = 21/45 (46%) Frame = +2 Query: 275 GEKNGFRRRSTYPPLSASILSNFEENFESLIDNLTDADSLTSSTE 409 GEK G RS + SA L + E NF S + D + + S + Sbjct: 464 GEKVGIEYRSFFQKCSAEFLCSNEPNFRSQLTEFFDHNIIESKRD 508 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 27.9 bits (59), Expect = 1.5 Identities = 18/75 (24%), Positives = 35/75 (46%) Frame = +2 Query: 161 ASDSEPTSLPSLPQEDKVAEETGDRIGNYSNEVNHVPIGEKNGFRRRSTYPPLSASILSN 340 A+ EP + +L +E + + + GNY +VN + + E+ R+ Y +S + Sbjct: 345 ANSQEPKLISNLAKEMGIYDTELENYGNYKAKVN-LAVYERLKHRKDGNYVVVSGITPTP 403 Query: 341 FEENFESLIDNLTDA 385 F E +++ L A Sbjct: 404 FGEGKSTVVAGLVQA 418 >SPBC902.02c |ctf18|chl12|DNA replication factor C complex subunit Ctf18|Schizosaccharomyces pombe|chr 2|||Manual Length = 960 Score = 27.5 bits (58), Expect = 1.9 Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 2/61 (3%) Frame = -1 Query: 535 ATHDLNHNSVPNNAFLSIQELGVA*FITAGAVAVTHTLEHFSFGARSQTVRIS--EVINQ 362 A +L H S+PN+ F S Q +G T + TL+ +S + IS E INQ Sbjct: 44 ALENLEHRSIPNDLFHSSQPVGSP---TRNGDDIPSTLDLYSSDNAAIDTDISEDETINQ 100 Query: 361 R 359 R Sbjct: 101 R 101 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 27.5 bits (58), Expect = 1.9 Identities = 20/76 (26%), Positives = 33/76 (43%) Frame = -2 Query: 231 SPVSSATLSSWGNDGREVGSESEAAVLAVSHPEETDPSSLSVTRKLLDNLHSVTFAAAVS 52 S SS++ SS + S S + S + SS S + S +F +S Sbjct: 186 SSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSSFITTMS 245 Query: 51 IISFVSTMLLVPNSCS 4 +F+ST+ + P+S S Sbjct: 246 SSTFISTVTVTPSSSS 261 >SPBP18G5.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 430 Score = 26.6 bits (56), Expect = 3.4 Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 1/70 (1%) Frame = +2 Query: 296 RRSTYPPLSASILSNFEENFESLIDN-LTDADSLTSSTETEVFKSVGDSDSACSDESGYS 472 +RST + + N + N+E+ L DA ++ S E EV K++ DS+ A ES S Sbjct: 153 KRSTNRLSLLAFMRNQQLNYEAYETKLLEDASTIEKSLEKEV-KTIYDSNIASPKES--S 209 Query: 473 EFLDGKESII 502 + +D ++I Sbjct: 210 DAIDADHAMI 219 >SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 26.6 bits (56), Expect = 3.4 Identities = 18/66 (27%), Positives = 33/66 (50%), Gaps = 1/66 (1%) Frame = +2 Query: 236 IGNYSNEVNHVPIGEKNGFRRRSTYPPLSASILSN-FEENFESLIDNLTDADSLTSSTET 412 IGN ++ + I + R R T PP S++ N +N+E L N+ +++ S E+ Sbjct: 231 IGNRDSKDTSISIQVERYPRTRLTKPPTSSAFYENDMSKNYECL--NIENSNVENKSMES 288 Query: 413 EVFKSV 430 + +V Sbjct: 289 DAVSTV 294 >SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 1822 Score = 26.6 bits (56), Expect = 3.4 Identities = 20/63 (31%), Positives = 26/63 (41%), Gaps = 2/63 (3%) Frame = +2 Query: 248 SNEVNHVPIGEKNGFRRRSTYPPLSASILSNFEENFESL-IDNL-TDADSLTSSTETEVF 421 SN + HV R RS PLS +S L ID + T SL+ S F Sbjct: 1110 SNSIRHVSGSTSRSTRTRSLSKPLSPEAVSELMSTEVVLSIDRIFTQTSSLSGSAIVSFF 1169 Query: 422 KSV 430 K++ Sbjct: 1170 KAL 1172 >SPCC16A11.02 |utp13|SPCC63.16|U3 snoRNP-associated protein Utp13 |Schizosaccharomyces pombe|chr 3|||Manual Length = 777 Score = 26.6 bits (56), Expect = 3.4 Identities = 19/59 (32%), Positives = 25/59 (42%), Gaps = 1/59 (1%) Frame = +2 Query: 269 PIGEKNGFRRRSTYPPLSASILSNFEENFESLIDNLTDADSLT-SSTETEVFKSVGDSD 442 PIGEK F + P+ F+ N + L+ LTD T S T +F D D Sbjct: 3 PIGEKKRFELEKSIEPIYTGGPVAFDSNEKILVTALTDRIIGTRSETGERLFSIKKDED 61 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 26.6 bits (56), Expect = 3.4 Identities = 25/101 (24%), Positives = 38/101 (37%), Gaps = 5/101 (4%) Frame = +2 Query: 143 ETASTAASDSEPTSLPSLPQEDKVAEETGDRIGNYSNEVNHVPIGEKNGFRRRSTYPPLS 322 E + D + SL QED + + +G Y HV G R T P Sbjct: 52 ECIVVSMDDKSKKARVSLRQEDIL-----NALGKYEETFRHVDFGPVYAALRNETCPKKI 106 Query: 323 ASILSNFEENFESLIDNL-----TDADSLTSSTETEVFKSV 430 +ILS +N ++ + D +TSST+ +V Sbjct: 107 DAILSEVAKNPADYVERIGGNSNKDTIEITSSTKPHAQNAV 147 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 26.6 bits (56), Expect = 3.4 Identities = 23/88 (26%), Positives = 36/88 (40%), Gaps = 1/88 (1%) Frame = +2 Query: 221 ETGDRIGNYSNEVNHVPIGEKNGFRRRSTYPPLSASILSNFEENFESLIDNLTDADSLTS 400 + G +I YS H + + + T P+S L NF E + L D + Sbjct: 102 KNGKQISQYSGPRKHDALVKYMRKQLLPTVKPISKDTLENFVEKADDLAVVAFFKDQKLN 161 Query: 401 STETEVFKSVGDS-DSACSDESGYSEFL 481 T TEV + + D A SD+ ++ L Sbjct: 162 DTYTEVAEVMKDDFVFAASDDKELAKSL 189 >SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyces pombe|chr 2|||Manual Length = 585 Score = 26.2 bits (55), Expect = 4.4 Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Frame = +2 Query: 197 PQEDKVAEE-TGDRIGNYSNEVNHVPIGEKNGFRR 298 P++ K + G+ + +Y EV VP E+N FRR Sbjct: 26 PEKSKDKDNFNGEEVISYVGEVETVPAKEENVFRR 60 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.8 bits (54), Expect = 5.9 Identities = 20/72 (27%), Positives = 31/72 (43%) Frame = -2 Query: 219 SATLSSWGNDGREVGSESEAAVLAVSHPEETDPSSLSVTRKLLDNLHSVTFAAAVSIISF 40 S+TLSS S S + + + PSS S + + S +F++ VS S Sbjct: 318 SSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSFSSTTSSSKSSSSFSSTVSSSSS 377 Query: 39 VSTMLLVPNSCS 4 S+ L +S S Sbjct: 378 TSSSTLTSSSSS 389 >SPAC3A11.09 |sod22||plasma membrane alkali metal cation/H+ antiporter Sod22|Schizosaccharomyces pombe|chr 1|||Manual Length = 759 Score = 25.8 bits (54), Expect = 5.9 Identities = 7/20 (35%), Positives = 11/20 (55%) Frame = +1 Query: 103 RHGQRRWICFFWMRDCQYRC 162 RH R W+C + +C + C Sbjct: 197 RHAGRDWVCIIILYECTFGC 216 >SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 25.8 bits (54), Expect = 5.9 Identities = 24/100 (24%), Positives = 48/100 (48%), Gaps = 5/100 (5%) Frame = +2 Query: 293 RRRSTYPPLSASILSNFEENFESLIDNLTDADSLTSSTETEVFKSVGDSDSACSDESGYS 472 R +S YP L+ S +S +NF + D L + S + +G ++++ S+ + Sbjct: 508 RLKSLYPKLALSQMSQLVKNFYAFEDPLLQIEK--SLVLNKPIHQIG-AEASSSNNVKST 564 Query: 473 EFLDG-----KESIIGNTIMV*IMCRDFMKIGADYKFTVT 577 E L+G K ++ + + + DF+ + A+Y +T T Sbjct: 565 ELLEGLANARKSALHEIFVKINLKIDDFLGL-AEYDWTTT 603 >SPCC126.05c |mrpl17||mitochondrial ribosomal protein subunit L17|Schizosaccharomyces pombe|chr 3|||Manual Length = 268 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/37 (35%), Positives = 18/37 (48%) Frame = +3 Query: 405 PKLKCSRVWVTATAPAVMNQATPSSWMERKALLGTLL 515 P L+ WV T + N+ +PSSW K +L L Sbjct: 232 PNLQNVYDWVWCTYDELKNKLSPSSWDSVKNILSDRL 268 >SPAPB8E5.09 |||AAA family ATPase Rvb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 25.4 bits (53), Expect = 7.8 Identities = 19/84 (22%), Positives = 36/84 (42%) Frame = +2 Query: 206 DKVAEETGDRIGNYSNEVNHVPIGEKNGFRRRSTYPPLSASILSNFEENFESLIDNLTDA 385 + V EE + +G Y ++HV +G K + + L SI + + + ++ Sbjct: 138 EMVPEEAENPLGGYGKTISHVLLGLKT--HKGTKQLKLDPSIYESLQR------EQVSTG 189 Query: 386 DSLTSSTETEVFKSVGDSDSACSD 457 D + T K VG SD+ ++ Sbjct: 190 DVIYIEANTGAVKRVGRSDAYATE 213 >SPAC1002.10c |sgt1||SGT1 family transcriptional regulator Sgt1|Schizosaccharomyces pombe|chr 1|||Manual Length = 590 Score = 25.4 bits (53), Expect = 7.8 Identities = 24/74 (32%), Positives = 36/74 (48%), Gaps = 3/74 (4%) Frame = +2 Query: 281 KNGFRRRSTYPPLSASILSNFEENFESL--IDNLTDADSLTSSTETEVFKSVGDS-DSAC 451 KN R T+ AS +N E+F + D TD+DSL S + E+F + D Sbjct: 425 KNMASRIETFINDEAS--NNHREDFYGVKNSDTDTDSDSLADS-DDEIFLNRNQGIDEVE 481 Query: 452 SDESGYSEFLDGKE 493 DE+ + + L GK+ Sbjct: 482 FDETKFYDLLKGKD 495 >SPAC589.08c |dam1||DASH complex subunit Dam1|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +2 Query: 290 FRRRSTYPPLSASILSNFEENFESLIDNL 376 FR+R + + IL NF E+F S + L Sbjct: 47 FRKRMNHLSATKQILDNFNESFSSFLYGL 75 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,583,948 Number of Sequences: 5004 Number of extensions: 50576 Number of successful extensions: 211 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 211 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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